MytuD.01188.a

PROBABLE DIOXYGENASE

CENTER ID: MytuD.01188.a
ORGANISM: Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE: Tuberculosis
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:
IIIC

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Proteins

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
MytuD.01188.a.A1.PS00170b Full length( MytuD.01188.a ) 1 295
External Resources
RESOURCE REFERENCE ID
OrthoMCL: OG5_129515
PATRIC ID: fig|83332.12.peg.3803
RefSeq: NP_217923.1
TubercuList: Rv3406
MTB Network Portal: Rv3406
UniProt: P9WKZ1
Orthologues for Rv3406
Species % Identity % Coverage Clones Proteins Structures
marinum 84 100 1 1 1
avium 80 99 0 1 1
smegmatis 63 96 1 1 1
abscessus 54 99 1 1 1
abscessus 53 98 1 1 0
avium 33 93 1 1 0
paratuberculosis 33 93 1 1 0
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTDLITVKKL GSRIGAQIDG VRLGGDLDPA AVNEIRAALL AHKVVFFRGQ HQLDDAEQLA FAGLLGTPIG HPAAIALADD APIITPINSE FGKANRWHTD VTFAANYPAA SVLRAVSLPS YGGSTLWANT AAAYAELPEP LKCLTENLWA LHTNRYDYVT TKPLTAAQRA FRQVFEKPDF RTEHPVVRVH PETGERTLLA GDFVRSFVGL DSHESRVLFE VLQRRITMPE NTIRWNWAPG DVAIWDNRAT QHRAIDDYDD QHRLMHRVTL MGDVPVDVYG QASRVISGAP MEIAG
NT Sequence
ATGACAGATC TGATTACCGT GAAGAAGCTG GGCAGCCGTA TCGGCGCCCA AATCGACGGG GTGCGCCTCG GAGGCGATCT GGACCCCGCC GCAGTCAACG AGATTCGCGC GGCACTACTG GCCCACAAGG TGGTCTTCTT CCGCGGTCAG CACCAACTCG ATGACGCCGA GCAGCTGGCG TTTGCCGGGT TACTGGGCAC CCCGATCGGC CACCCGGCCG CGATCGCCCT CGCCGACGAT GCACCGATCA TCACGCCGAT CAACTCCGAG TTCGGCAAGG CGAACCGCTG GCACACCGAC GTCACGTTCG CCGCCAACTA TCCGGCCGCC TCGGTACTGC GCGCGGTCTC CCTGCCCAGC TATGGCGGGT CGACGTTGTG GGCCAACACC GCCGCGGCCT ACGCGGAGCT GCCCGAGCCG CTCAAGTGCC TCACCGAAAA CCTGTGGGCG CTGCACACCA ACCGCTATGA CTACGTCACG ACCAAACCGC TGACCGCGGC GCAGCGGGCC TTCCGTCAGG TGTTCGAGAA GCCGGACTTC CGCACCGAGC ATCCCGTGGT GCGGGTACAC CCGGAGACCG GTGAGCGCAC GCTGCTAGCG GGCGACTTCG TGCGCAGCTT CGTCGGGTTG GACAGCCACG AATCAAGGGT GTTATTCGAA GTGCTGCAAC GGCGAATCAC CATGCCCGAA AACACCATCC GCTGGAACTG GGCGCCGGGC GACGTAGCCA TCTGGGACAA CCGGGCCACC CAACACCGGG CGATCGACGA CTACGACGAC CAGCACCGGC TGATGCACCG GGTCACCTTG ATGGGCGACG TGCCCGTCGA CGTGTACGGG CAGGCTAGCC GGGTGATCAG CGGGGCGCCG ATGGAGATCG CTGGC
Details for MytuD.01188.a.A1.PS00170b
PURIFICATION DATe: 4/2/2009
CONCENTRATION: 56.13mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 15
VIAL VOLUME: 50µl
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTDLITVKK LGSRIGAQID GVRLGGDLDP AAVNEIRAAL LAHKVVFFRG QHQLDDAEQL AFAGLLGTPI GHPAAIALAD DAPIITPINS EFGKANRWHT DVTFAANYPA ASVLRAVSLP SYGGSTLWAN TAAAYAELPE PLKCLTENLW ALHTNRYDYV TTKPLTAAQR AFRQVFEKPD FRTEHPVVRV HPETGERTLL AGDFVRSFVG LDSHESRVLF EVLQRRITMP ENTIRWNWAP GDVAIWDNRA TQHRAIDDYD DQHRLMHRVT LMGDVPVDVY GQASRVISGA PMEIAG
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GACAGATCTG ATTACCGTGA AGAAGCTGGG CAGCCGTATC GGCGCCCAAA TCGACGGGGT GCGCCTCGGA GGCGATCTGG ACCCCGCCGC AGTCAACGAG ATTCGCGCGG CACTACTGGC CCACAAGGTG GTCTTCTTCC GCGGTCAGCA CCAACTCGAT GACGCCGAGC AGCTGGCGTT TGCCGGGTTA CTGGGCACCC CGATCGGCCA CCCGGCCGCG ATCGCCCTCG CCGACGATGC ACCGATCATC ACGCCGATCA ACTCCGAGTT CGGCAAGGCG AACCGCTGGC ACACCGACGT CACGTTCGCC GCCAACTATC CGGCCGCCTC GGTACTGCGC GCGGTCTCCC TGCCCAGCTA TGGCGGGTCG ACGTTGTGGG CCAACACCGC CGCGGCCTAC GCGGAGCTGC CCGAGCCGCT CAAGTGCCTC ACCGAAAACC TGTGGGCGCT GCACACCAAC CGCTATGACT ACGTCACGAC CAAACCGCTG ACCGCGGCGC AGCGGGCCTT CCGTCAGGTG TTCGAGAAGC CGGACTTCCG CACCGAGCAT CCCGTGGTGC GGGTACACCC GGAGACCGGT GAGCGCACGC TGCTAGCGGG CGACTTCGTG CGCAGCTTCG TCGGGTTGGA CAGCCACGAA TCAAGGGTGT TATTCGAAGT GCTGCAACGG CGAATCACCA TGCCCGAAAA CACCATCCGC TGGAACTGGG CGCCGGGCGA CGTAGCCATC TGGGACAACC GGGCCACCCA ACACCGGGCG ATCGACGACT ACGACGACCA GCACCGGCTG ATGCACCGGG TCACCTTGAT GGGCGACGTG CCCGTCGACG TGTACGGGCA GGCTAGCCGG GTGATCAGCG GGGCGCCGAT GGAGATCGCT GGCAAACAGC ACGAACAAGT TCTGCAGCCA AGCTTCTCGA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAA