MysmA.01188.a
Alpha-ketoglutarate-dependent taurine dioxygenase
| CENTER ID: | MysmA.01188.a |
| ORGANISM: | Mycobacterium smegmatis ATCC 700084 / mc(2)155 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIC |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MysmA.01188.a.A1.GE28885 | Full length( MysmA.01188.a ) | 1 | 299 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MysmA.01188.a.A1.PS00650 | Full length( MysmA.01188.a ) | 1 | 299 |
Structures
4J5I
DEPOSITED: 2/8/2013
DETERMINATION: XRay
CLONE: MysmA.01188.a.A1.GE28885
PROTEIN: MysmA.01188.a.A1.PS00650
Publications by SSGCID
Increasing the structural coverage of tuberculosis drug targets.
Abendroth J, Abramov A, Armour B, Barrett LK, Baugh L, Begley DW, Buchko GW, Choi R, Clifton MC, Dieterich SH, Dranow DM, Edwards TE, Fairman JW, Ferrell M, Fox D, Gardberg AS, Hewitt SN, Lorimer D, Lyons-Abbott S, Mundt E, Muruthi MM, Myers J, Myler PJ, Napuli AJ, Phan I, Sekar A, Serbzhinskiy D, Stacy R, Staker BL, Stewart LJ, Taylor BM, Thompkins K, Tran N, Van Voorhis WC, Zhang Y
Tuberculosis (Edinb) - 2014
volume 95, issue 2, pages 142-8
PMID: 25613812; PMCID: PMC4361283
PDB:
3GVC,
3GVG,
3GWC,
3H7F,
3H81,
3HE2,
3HWI,
3HWK,
3HZG,
3ICO,
3KHP,
3LLS,
3MOY,
3MPZ,
3MYB,
3NDN,
3NDO,
3NF4,
3NG3,
3NJD,
3NWO,
3O0M,
3O38,
3OC6,
3OC7,
3OEC,
3OI9,
3OKS,
3OL3,
3OL4,
3OME,
3P0T,
3P2Y,
3P4I,
3P4T,
3P5M,
3P85,
3PE8,
3PGX,
3PK0,
3PPI,
3PXX,
3PZY,
3Q1T,
3Q8N,
3QBP,
3QDF,
3QH4,
3QHA,
3QIV,
3QK8,
3QKA,
3QLJ,
3QMJ,
3QRE,
3QUA,
3QUV,
3QXI,
3QXZ,
3QYR,
3R0O,
3R1I,
3R1J,
3R20,
3R2N,
3R4T,
3R6H,
3R6O,
3R7K,
3R8C,
3R9P,
3R9Q,
3R9R,
3R9S,
3R9T,
3RD5,
3RD7,
3RD8,
3RFQ,
3RIH,
3RR2,
3RR6,
3RRP,
3RRV,
3RSI,
3RV2,
3S55,
3S82,
3SBX,
3SF6,
3SLL,
3SVK,
3SVT,
3SWO,
3SWT,
3SWX,
3SX2,
3T3W,
3T7C,
3TAV,
3TCR,
3TDE,
3TJR,
3TL3,
3TLF,
3TRR,
3TSC,
3TX2,
3TZQ,
3TZU,
3U0A,
3U0B,
3UCX,
3UVE,
4DI1,
4DIE,
4DQ8,
4DXL,
4ED4,
4EGE,
4EGF,
4EMD,
4EO9,
4EYE,
4F3W,
4F47,
4FFC,
4GK6,
4HDT,
4HR3,
4I1Y,
4IJN,
4IV6,
4IZ9,
4J5I,
4KAM,
4LGV,
4O2D,
5EJ2
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| BV-BRC: | fig|246196.19.peg.3809 |
| RefSeq: | YP_888161.1 |
| UniProt: | A0QZ23 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MQVTVTKLGA HIGARIDGVR VGGDLSPATV SAINAALLEH KVIFFSGQDH LDDAGQLEFA ELLGTPTVAH PTLAEGAEQL LPIDSRYDKA NSWHTDVTFV DRIPKASLLR AVTLPSYGGT TAWASTEAAY QQLPAPLRTL ADNLWAVHTN RFDYADSAIS AEQRGYRQRF ESDYYEVEHP VVRVHPETGE RVLLLGHFVK SFVGLKDTES AALFRLFQDR ITRLENTVRW SWKPGDLAIW DNRATQHYAV ADYDDQYRRL NRVTLAGDIP VDVYGERSRV IAGDASSYSP VDSPVALAS
NT Sequence
GTGCAGGTCA CCGTGACCAA GCTCGGGGCC CACATCGGCG CCAGGATCGA CGGCGTGCGC GTCGGCGGCG ACCTGTCCCC CGCCACCGTG AGCGCCATCA ACGCGGCCCT CCTCGAGCAC AAGGTGATCT TCTTCTCCGG CCAGGACCAT CTCGACGATG CCGGACAGCT GGAGTTCGCC GAGCTGCTGG GTACGCCCAC CGTCGCCCAT CCGACGCTCG CCGAGGGCGC CGAGCAGTTG CTGCCGATCG ACTCGCGCTA CGACAAGGCC AACAGCTGGC ACACCGATGT CACGTTCGTC GACCGTATCC CGAAGGCGTC GCTGCTGCGC GCGGTGACGC TGCCCAGCTA CGGCGGCACC ACCGCGTGGG CCTCGACGGA AGCGGCCTAC CAGCAGCTGC CCGCGCCGCT GCGCACGCTG GCCGACAACC TGTGGGCCGT GCACACCAAC CGGTTCGACT ACGCCGACAG CGCGATCTCC GCGGAACAGC GTGGCTACCG TCAGCGGTTC GAATCCGACT ATTACGAGGT CGAGCACCCG GTGGTGCGGG TGCACCCCGA GACCGGTGAG CGGGTCCTGC TCCTCGGCCA TTTCGTCAAG TCGTTCGTCG GACTCAAGGA CACCGAGTCC GCGGCTTTGT TCCGGCTGTT CCAGGACCGG ATCACGCGGC TTGAGAACAC CGTACGGTGG AGCTGGAAGC CCGGTGACCT GGCGATCTGG GACAACCGCG CCACGCAGCA CTACGCGGTG GCCGACTACG ACGACCAGTA CCGCAGGCTC AACCGCGTCA CGCTGGCCGG TGACATCCCG GTCGACGTGT ACGGCGAACG CAGCCGCGTC ATCGCCGGTG ACGCGTCGAG TTACTCCCCG GTGGACAGCC CGGTCGCCCT GGCGAGC
Details for MysmA.01188.a.A1.GE28885
| HARVESTED ON: | 3/23/2010 |
| SEQUENCED ON: | 4/13/2010 |
| EXPECTED MW: | 35kDa |
| OBSERVED MW: | 36kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVQVTVTKL GAHIGARIDG VRVGGDLSPA TVSAINAALL EHKVIFFSGQ DHLDDAGQLE FAELLGTPTV AHPTLAEGAE QLLPIDSRYD KANSWHTDVT FVDRIPKASL LRAVTLPSYG GTTAWASTEA AYQQLPAPLR TLADNLWAVH TNRFDYADSA ISAEQRGYRQ RFESDYYEVE HPVVRVHPET GERVLLLGHF VKSFVGLKDT ESAALFRLFQ DRITRLENTV RWSWKPGDLA IWDNRATQHY AVADYDDQYR RLNRVTLAGD IPVDVYGERS RVIAGDASSY SPVDSPVALA S
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggtgc aggtcaccgt gaccaagctc ggggcccaca tcggcgccag gatcgacggc gtgcgcgtcg gcggcgacct gtcccccgcc accgtgagcg ccatcaacgc ggccctcctc gagcacaagg tgatcttctt ctccggccag gaccatctcg acgatgccgg acagctggag ttcgccgagc tgctgggtac gcccaccgtc gcccatccga cgctcgccga gggcgccgag cagttgctgc cgatcgactc gcgctacgac aaggccaaca gctggcacac cgatgtcacg ttcgtcgacc gtatcccgaa ggcgtcgctg ctgcgcgcgg tgacgctgcc cagctacggc ggcaccaccg cgtgggcctc gacggaagcg gcctaccagc agctgcccgc gccgctgcgc acgctggccg acaacctgtg ggccgtgcac accaaccggt tcgactacgc cgacagcgcg atctccgcgg aacagcgtgg ctaccgtcag cggttcgaat ccgactatta cgaggtcgag cacccggtgg tgcgggtgca ccccgagacc ggtgagcggg tcctgctcct cggccatttc gtcaagtcgt tcgtcggact caaggacacc gagtccgcgg ctttgttccg gctgttccag gaccggatca cgcggcttga gaacaccgta cggtggagct ggaagcccgg tgacctggcg atctgggaca accgcgccac gcagcactac gcggtggccg actacgacga ccagtaccgc aggctcaacc gcgtcacgct ggccggtgac atcccggtcg acgtgtacgg cgaacgcagc cgcgtcatcg ccggtgacgc gtcgagttac tccccggtgg acagcccggt cgccctggcg agctaataa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVQVTVTKL GAHIGARIDG VRVGGDLSPA TVSAINAALL EHKVIFFSGQ DHLDDAGQLE FAELLGTPTV AHPTLAEGAE QLLPIDSRYD KANSWHTDVT FVDRIPKASL LRAVTLPSYG GTTAWASTEA AYQQLPAPLR TLADNLWAVH TNRFDYADSA ISAEQRGYRQ RFESDYYEVE HPVVRVHPET GERVLLLGHF VKSFVGLKDT ESAALFRLFQ DRITRLENTV RWSWKPGDLA IWDNRATQHY AVADYDDQYR RLNRVTLAGD IPVDVYGERS RVIAGDASSY SPVDSPVALA S
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgcaggtc accgtgacca agctcggggc ccacatcggc gccaggatcg acggcgtgcg cgtcggcggc gacctgtccc ccgccaccgt gagcgccatc aacgcggccc tcctcgagca caaggtgatc ttcttctccg gccaggacca tctcgacgat gccggacagc tggagttcgc cgagctgctg ggtacgccca ccgtcgccca tccgacgctc gccgagggcg ccgagcagtt gctgccgatc gactcgcgct acgacaaggc caacagctgg cacaccgatg tcacgttcgt cgaccgtatc ccgaaggcgt cgctgctgcg cgcggtgacg ctgcccagct acggcggcac caccgcgtgg gcctcgacgg aagcggccta ccagcagctg cccgcgccgc tgcgcacgct ggccgacaac ctgtgggccg tgcacaccaa ccggttcgac tacgccgaca gcgcgatctc cgcggaacag cgtggctacc gtcagcggtt cgaatccgac tattacgagg tcgagcaccc ggtggtgcgg gtgcaccccg agaccggtga gcgggtcctg ctcctcggcc atttcgtcaa gtcgttcgtc ggactcaagg acaccgagtc cgcggctttg ttccggctgt tccaggaccg gatcacgcgg cttgagaaca ccgtacggtg gagctggaag cccggtgacc tggcgatctg ggacaaccgc gccacgcagc actacgcggt ggccgactac gacgaccagt accgcaggct caaccgcgtc acgctggccg gtgacatccc ggtcgacgtg tacggcgaac gcagccgcgt catcgccggt gacgcgtcga gttactcccc ggtggacagc ccggtcgccc tggcgagcaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa
Details for MysmA.01188.a.A1.PS00650
| PURIFICATION DATe: | 6/23/2010 |
| CONCENTRATION: | 190mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 3 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVQVTVTKL GAHIGARIDG VRVGGDLSPA TVSAINAALL EHKVIFFSGQ DHLDDAGQLE FAELLGTPTV AHPTLAEGAE QLLPIDSRYD KANSWHTDVT FVDRIPKASL LRAVTLPSYG GTTAWASTEA AYQQLPAPLR TLADNLWAVH TNRFDYADSA ISAEQRGYRQ RFESDYYEVE HPVVRVHPET GERVLLLGHF VKSFVGLKDT ESAALFRLFQ DRITRLENTV RWSWKPGDLA IWDNRATQHY AVADYDDQYR RLNRVTLAGD IPVDVYGERS RVIAGDASSY SPVDSPVALA S
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggtgc aggtcaccgt gaccaagctc ggggcccaca tcggcgccag gatcgacggc gtgcgcgtcg gcggcgacct gtcccccgcc accgtgagcg ccatcaacgc ggccctcctc gagcacaagg tgatcttctt ctccggccag gaccatctcg acgatgccgg acagctggag ttcgccgagc tgctgggtac gcccaccgtc gcccatccga cgctcgccga gggcgccgag cagttgctgc cgatcgactc gcgctacgac aaggccaaca gctggcacac cgatgtcacg ttcgtcgacc gtatcccgaa ggcgtcgctg ctgcgcgcgg tgacgctgcc cagctacggc ggcaccaccg cgtgggcctc gacggaagcg gcctaccagc agctgcccgc gccgctgcgc acgctggccg acaacctgtg ggccgtgcac accaaccggt tcgactacgc cgacagcgcg atctccgcgg aacagcgtgg ctaccgtcag cggttcgaat ccgactatta cgaggtcgag cacccggtgg tgcgggtgca ccccgagacc ggtgagcggg tcctgctcct cggccatttc gtcaagtcgt tcgtcggact caaggacacc gagtccgcgg ctttgttccg gctgttccag gaccggatca cgcggcttga gaacaccgta cggtggagct ggaagcccgg tgacctggcg atctgggaca accgcgccac gcagcactac gcggtggccg actacgacga ccagtaccgc aggctcaacc gcgtcacgct ggccggtgac atcccggtcg acgtgtacgg cgaacgcagc cgcgtcatcg ccggtgacgc gtcgagttac tccccggtgg acagcccggt cgccctggcg agctaataa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVQVTVTKL GAHIGARIDG VRVGGDLSPA TVSAINAALL EHKVIFFSGQ DHLDDAGQLE FAELLGTPTV AHPTLAEGAE QLLPIDSRYD KANSWHTDVT FVDRIPKASL LRAVTLPSYG GTTAWASTEA AYQQLPAPLR TLADNLWAVH TNRFDYADSA ISAEQRGYRQ RFESDYYEVE HPVVRVHPET GERVLLLGHF VKSFVGLKDT ESAALFRLFQ DRITRLENTV RWSWKPGDLA IWDNRATQHY AVADYDDQYR RLNRVTLAGD IPVDVYGERS RVIAGDASSY SPVDSPVALA S
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGCAGGTC ACCGTGACCA AGCTCGGGGC CCACATCGGC GCCAGGATCG ACGGCGTGCG CGTCGGCGGC GACCTGTCCC CCGCCACCGT GAGCGCCATC AACGCGGCCC TCCTCGAGCA CAAGGTGATC TTCTTCTCCG GCCAGGACCA TCTCGACGAT GCCGGACAGC TGGAGTTCGC CGAGCTGCTG GGTACGCCCA CCGTCGCCCA TCCGACGCTC GCCGAGGGCG CCGAGCAGTT GCTGCCGATC GACTCGCGCT ACGACAAGGC CAACAGCTGG CACACCGATG TCACGTTCGT CGACCGTATC CCGAAGGCGT CGCTGCTGCG CGCGGTGACG CTGCCCAGCT ACGGCGGCAC CACCGCGTGG GCCTCGACGG AAGCGGCCTA CCAGCAGCTG CCCGCGCCGC TGCGCACGCT GGCCGACAAC CTGTGGGCCG TGCACACCAA CCGGTTCGAC TACGCCGACA GCGCGATCTC CGCGGAACAG CGTGGCTACC GTCAGCGGTT CGAATCCGAC TATTACGAGG TCGAGCACCC GGTGGTGCGG GTGCACCCCG AGACCGGTGA GCGGGTCCTG CTCCTCGGCC ATTTCGTCAA GTCGTTCGTC GGACTCAAGG ACACCGAGTC CGCGGCTTTG TTCCGGCTGT TCCAGGACCG GATCACGCGG CTTGAGAACA CCGTACGGTG GAGCTGGAAG CCCGGTGACC TGGCGATCTG GGACAACCGC GCCACGCAGC ACTACGCGGT GGCCGACTAC GACGACCAGT ACCGCAGGCT CAACCGCGTC ACGCTGGCCG GTGACATCCC GGTCGACGTG TACGGCGAAC GCAGCCGCGT CATCGCCGGT GACGCGTCGA GTTACTCCCC GGTGGACAGC CCGGTCGCCC TGGCGAGCAA ACAGCACGAA CAAGTTCTGC AGCCAAGCTT CTCGAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA