SSGCID: Target Page

MypaA.01188.a

Putative uncharacterized protein

CENTER ID:	MypaA.01188.a
ORGANISM:	Mycobacterium paratuberculosis ATCC BAA-968 / K-10
ASSOCIATED DISEASE:	Johne's disease
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

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Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MypaA.01188.a.A1.GE29557	Full length( MypaA.01188.a )		1	281

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MypaA.01188.a.A1.PS00731	Full length( MypaA.01188.a )		1	281

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|262316.17.peg.3322
RefSeq:	NP_962069.1
UniProt:	Q73V79

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSLLTITKLT DSVGAEVTGL DPAALAHDDS VGEAVLDALE DNGVLVFRGL YLDPAAQVAF CGRLGEVDHS SDGHHPVPGI YPITLDKSKN ASAAYLKATF DWHIDGCTPL GDECPQKATV LSAVRVAERG GETEFANSYA AYDALTDDEK RRFGALRVVH SLEASQRRVY PDPSPELMAR WRSRRTHEHP LVWTHRSGRK SLVLGASADY VVGMDVDEGR ALLEELLQRA TVPERVYRHG WSIGDTVIWD NRGVLHRAAP YDPDSSREML RTTVRGDEPI Q

NT Sequence

ATGAGTCTGC TCACCATCAC CAAACTCACC GACTCGGTCG GCGCCGAAGT CACCGGCCTG GATCCGGCCG CCCTGGCACA CGACGACTCG GTGGGTGAGG CGGTGCTGGA TGCCTTGGAA GACAACGGGG TTCTGGTCTT CCGCGGCTTG TATCTCGACC CGGCGGCGCA GGTCGCCTTC TGCGGCCGCC TCGGCGAGGT CGATCACTCC TCCGACGGCC ACCACCCGGT GCCCGGCATC TACCCGATCA CGCTGGACAA GTCCAAGAAC GCCTCCGCGG CCTACCTGAA GGCGACCTTC GACTGGCACA TCGACGGCTG CACACCGCTG GGCGACGAGT GTCCGCAGAA GGCCACCGTG CTGTCGGCGG TCCGGGTCGC CGAGCGGGGC GGCGAAACCG AATTCGCGAA TTCCTACGCT GCGTACGACG CACTGACCGA CGACGAGAAA CGGCGGTTCG GCGCATTGCG GGTGGTGCAC TCGCTGGAGG CCTCCCAGCG CCGGGTCTAT CCCGATCCGT CACCGGAACT CATGGCGCGC TGGCGGTCTC GGCGCACGCA TGAACACCCG CTGGTGTGGA CGCATCGCAG CGGGCGCAAA TCGCTGGTGC TGGGTGCCTC CGCGGATTAC GTCGTGGGCA TGGACGTCGA CGAGGGACGC GCCCTGCTCG AGGAACTGCT GCAGCGCGCC ACGGTGCCCG AACGGGTCTA CCGCCACGGC TGGTCGATCG GCGACACCGT CATCTGGGAC AACCGTGGGG TGCTGCACCG GGCGGCGCCC TACGACCCGG ACTCGTCGCG AGAGATGCTG CGCACCACCG TGCGCGGTGA CGAGCCGATC CAA

Details for MypaA.01188.a.A1.GE29557

HARVESTED ON:	6/15/2010
SEQUENCED ON:	6/27/2010
EXPECTED MW:	33kDa
OBSERVED MW:	33kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Validated AA Sequence

MGTLEAQTQG PGSMSLLTIT KLTDSVGAEV TGLDPAALAH DDSVGEAVLD ALEDNGVLVF RGLYLDPAAQ VAFCGRLGEV DHSSDGHHPV PGIYPITLDK SKNASAAYLK ATFDWHIDGC TPLGDECPQK ATVLSAVRVA ERGGETEFAN SYAAYDALTD DEKRRFGALR VVHSLEASQR RVYPDPSPEL MARWRSRRTH EHPLVWTHRS GRKSLVLGAS ADYVVGMDVD EGRALLEELL QRATVPERVY RHGWSIGDTV IWDNRGVLHR AAPYDPDSSR EMLRTTVRGD EPIQ

Validated NT Sequence

ttattattgg atcggctcgt caccgcgcac ggtggtgcgc agcatctctc gcgacgagtc cgggtcgtag ggcgccgccc ggtgcagcac cccacggttg tcccagatga cggtgtcgcc gatcgaccag ccgtggcggt agacccgttc gggcaccgtg gcgcgctgca gcagttcctc gagcagggcg cgtccctcgt cgacgtccat gcccacgacg taatccgcgg aggcacccag caccagcgat ttgcgcccgc tgcgatgcgt ccacaccagc gggtgttcat gcgtgcgccg agaccgccag cgcgccatga gttccggtga cggatcggga tagacccggc gctgggaggc ctccagcgag tgcaccaccc gcaatgcgcc gaaccgccgt ttctcgtcgt cggtcagtgc gtcgtacgca gcgtaggaat tcgcgaattc ggtttcgccg ccccgctcgg cgacccggac cgccgacagc acggtggcct tctgcggaca ctcgtcgccc agcggtgtgc agccgtcgat gtgccagtcg aaggtcgcct tcaggtaggc cgcggaggcg ttcttggact tgtccagcgt gatcgggtag atgccgggca ccgggtggtg gccgtcggag gagtgatcga cctcgccgag gcggccgcag aaggcgacct gcgccgccgg gtcgagatac aagccgcgga agaccagaac cccgttgtct tccaaggcat ccagcaccgc ctcacccacc gagtcgtcgt gtgccagggc ggccggatcc aggccggtga cttcggcgcc gaccgagtcg gtgagtttgg tgatggtgag cagactcatc gaaccaggac cctgggtctg agcttccagg gtacccatat gatggtgatg gtgat

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMSLLTITKL TDSVGAEVTG LDPAALAHDD SVGEAVLDAL EDNGVLVFRG LYLDPAAQVA FCGRLGEVDH SSDGHHPVPG IYPITLDKSK NASAAYLKAT FDWHIDGCTP LGDECPQKAT VLSAVRVAER GGETEFANSY AAYDALTDDE KRRFGALRVV HSLEASQRRV YPDPSPELMA RWRSRRTHEH PLVWTHRSGR KSLVLGASAD YVVGMDVDEG RALLEELLQR ATVPERVYRH GWSIGDTVIW DNRGVLHRAA PYDPDSSREM LRTTVRGDEP IQ

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagtctgctc accatcacca aactcaccga ctcggtcggc gccgaagtca ccggcctgga tccggccgcc ctggcacacg acgactcggt gggtgaggcg gtgctggatg ccttggaaga caacggggtt ctggtcttcc gcggcttgta tctcgacccg gcggcgcagg tcgccttctg cggccgcctc ggcgaggtcg atcactcctc cgacggccac cacccggtgc ccggcatcta cccgatcacg ctggacaagt ccaagaacgc ctccgcggcc tacctgaagg cgaccttcga ctggcacatc gacggctgca caccgctggg cgacgagtgt ccgcagaagg ccaccgtgct gtcggcggtc cgggtcgccg agcggggcgg cgaaaccgaa ttcgcgaatt cctacgctgc gtacgacgca ctgaccgacg acgagaaacg gcggttcggc gcattgcggg tggtgcactc gctggaggcc tcccagcgcc gggtctatcc cgatccgtca ccggaactca tggcgcgctg gcggtctcgg cgcacgcatg aacacccgct ggtgtggacg catcgcagcg ggcgcaaatc gctggtgctg ggtgcctccg cggattacgt cgtgggcatg gacgtcgacg agggacgcgc cctgctcgag gaactgctgc agcgcgccac ggtgcccgaa cgggtctacc gccacggctg gtcgatcggc gacaccgtca tctgggacaa ccgtggggtg ctgcaccggg cggcgcccta cgacccggac tcgtcgcgag agatgctgcg caccaccgtg cgcggtgacg agccgatcca aaaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa

Details for MypaA.01188.a.A1.PS00731

PURIFICATION DATe:	8/24/2010
CONCENTRATION:	29.8mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	2
VIAL VOLUME:	data unavailable
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAGTCTGCTC ACCATCACCA AACTCACCGA CTCGGTCGGC GCCGAAGTCA CCGGCCTGGA TCCGGCCGCC CTGGCACACG ACGACTCGGT GGGTGAGGCG GTGCTGGATG CCTTGGAAGA CAACGGGGTT CTGGTCTTCC GCGGCTTGTA TCTCGACCCG GCGGCGCAGG TCGCCTTCTG CGGCCGCCTC GGCGAGGTCG ATCACTCCTC CGACGGCCAC CACCCGGTGC CCGGCATCTA CCCGATCACG CTGGACAAGT CCAAGAACGC CTCCGCGGCC TACCTGAAGG CGACCTTCGA CTGGCACATC GACGGCTGCA CACCGCTGGG CGACGAGTGT CCGCAGAAGG CCACCGTGCT GTCGGCGGTC CGGGTCGCCG AGCGGGGCGG CGAAACCGAA TTCGCGAATT CCTACGCTGC GTACGACGCA CTGACCGACG ACGAGAAACG GCGGTTCGGC GCATTGCGGG TGGTGCACTC GCTGGAGGCC TCCCAGCGCC GGGTCTATCC CGATCCGTCA CCGGAACTCA TGGCGCGCTG GCGGTCTCGG CGCACGCATG AACACCCGCT GGTGTGGACG CATCGCAGCG GGCGCAAATC GCTGGTGCTG GGTGCCTCCG CGGATTACGT CGTGGGCATG GACGTCGACG AGGGACGCGC CCTGCTCGAG GAACTGCTGC AGCGCGCCAC GGTGCCCGAA CGGGTCTACC GCCACGGCTG GTCGATCGGC GACACCGTCA TCTGGGACAA CCGTGGGGTG CTGCACCGGG CGGCGCCCTA CGACCCGGAC TCGTCGCGAG AGATGCTGCG CACCACCGTG CGCGGTGACG AGCCGATCCA AAAACAGCAC GAACAAGTTC TGCAGCCAAG CTTCTCGAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAA