MytuD.01113.a

PROBABLE CYSTEINE SYNTHASE B CYSM (CSASE B) (O-acetylserine sulfhydrylase B) (O-acetylserine (Thiol)-lyase B)

CENTER ID: MytuD.01113.a
ORGANISM: Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE: Tuberculosis
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MytuD.01113.a.A1.GE25938 Full length( MytuD.01113.a ) 1 323
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MytuD.01113.a.A1.PW26051 Full length( MytuD.01113.a ) 1 323

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|83332.12.peg.1497
RefSeq: NP_215852.1
TubercuList: Rv1336
MTB Network Portal: Rv1336
UniProt: P9WP53

Orthologues for Rv1336

Species % Identity % Coverage Clones Proteins Structures
smegmatis 88 100 1 1 0
abscessus 87 100 1 1 0
thermoresistibile 85 100 1 1 0
marinum 78 95 1 1 0
ulcerans 77 95 1 0 0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTRYDSLLQA LGNTPLVGLQ RLSPRWDDGR DGPHVRLWAK LEDRNPTGSI KDRPAVRMIE QAEADGLLRP GATILEPTSG NTGISLAMAA RLKGYRLICV MPENTSVERR QLLELYGAQI IFSAAEGGSN TAVATAKELA ATNPSWVMLY QYGNPANTDS HYCGTGPELL ADLPEITHFV AGLGTTGTLM GTGRFLREHV ANVKIVAAEP RYGEGVYALR NMDEGFVPEL YDPEILTARY SVGAVDAVRR TRELVHTEGI FAGISTGAVL HAALGVGAGA LAAGERADIA LVVADAGWKY LSTGAYAGSL DDAETALEGQ LWA
NT Sequence
ATGACACGAT ACGACTCGCT GTTGCAGGCC TTGGGCAACA CGCCGCTGGT TGGCCTGCAG CGATTGTCGC CACGCTGGGA TGACGGGCGA GACGGACCGC ACGTGCGGCT GTGGGCCAAG CTCGAGGACC GCAATCCGAC CGGGTCGATC AAGGACCGCC CGGCTGTGCG GATGATCGAG CAGGCCGAGG CCGACGGGTT GTTGCGGCCG GGCGCCACCA TCCTGGAGCC CACCAGCGGA AACACCGGCA TTTCGCTGGC GATGGCGGCC CGGTTGAAGG GGTACCGATT GATCTGCGTG ATGCCGGAGA ACACATCGGT TGAACGGCGG CAGCTGCTCG AGCTCTACGG CGCGCAGATT ATCTTCTCGG CGGCCGAAGG CGGGTCCAAC ACTGCGGTGG CCACCGCCAA AGAGCTGGCC GCGACCAACC CGTCATGGGT GATGCTGTAC CAGTACGGCA ATCCCGCCAA CACCGACTCG CACTACTGCG GCACCGGCCC CGAGCTGCTG GCCGACCTGC CCGAAATCAC GCACTTCGTC GCCGGCCTAG GCACCACGGG CACGCTGATG GGCACTGGCC GTTTCCTGCG CGAGCACGTT GCCAACGTCA AGATCGTGGC GGCCGAACCC CGCTACGGTG AGGGGGTATA CGCCCTGCGC AACATGGACG AAGGCTTTGT GCCCGAGCTG TATGACCCGG AAATACTGAC CGCGCGATAT TCTGTCGGCG CGGTGGACGC AGTGCGCCGC ACCCGCGAGT TGGTGCACAC CGAAGGCATC TTTGCGGGCA TCTCAACCGG CGCGGTGCTA CACGCCGCAC TCGGAGTCGG GGCCGGCGCC CTGGCGGCCG GCGAGCGGGC CGACATTGCG TTGGTGGTCG CCGACGCCGG GTGGAAGTAT CTGTCCACCG GCGCCTACGC CGGTAGCCTG GATGACGCCG AGACCGCTCT GGAAGGGCAA CTATGGGCA
Details for MytuD.01113.a.A1.GE25938
HARVESTED ON: 1/9/2009
SEQUENCED ON: 7/28/2010
EXPECTED MW: 36kDa
OBSERVED MW: 37kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTRYDSLLQ ALGNTPLVGL QRLSPRWDDG RDGPHVRLWA KLEDRNPTGS IKDRPAVRMI EQAEADGLLR PGATILEPTS GNTGISLAMA ARLKGYRLIC VMPENTSVER RQLLELYGAQ IIFSAAEGGS NTAVATAKEL AATNPSWVML YQYGNPANTD SHYCGTGPEL LADLPEITHF VAGLGTTGTL MGTGRFLREH VANVKIVAAE PRYGEGVYAL RNMDEGFVPE LYDPEILTAR YSVGAVDAVR RTRELVHTEG IFAGISTGAV LHAALGVGAG ALAAGERADI ALVVADAGWK YLSTGAYAGS LDDAETALEG QLWA
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgacac gatacgactc gctgttgcag gccttgggca acacgccgct ggttggcctg cagcgattgt cgccacgctg ggatgacggg cgagacggac cgcacgtgcg gctgtgggcc aagctcgagg accgcaatcc gaccgggtcg atcaaggacc gcccggctgt gcggatgatc gagcaggccg aggccgacgg gttgttgcgg ccgggcgcca ccatcctgga gcccaccagc ggaaacaccg gcatttcgct ggcgatggcg gcccggttga aggggtaccg attgatctgc gtgatgccgg agaacacatc ggttgaacgg cggcagctgc tcgagctcta cggcgcgcag attatcttct cggcggccga aggcgggtcc aacactgcgg tggccaccgc caaagagctg gccgcgacca acccgtcatg ggtgatgctg taccagtacg gcaatcccgc caacaccgac tcgcactact gcggcaccgg ccccgagctg ctggccgacc tgcccgaaat cacgcacttc gtcgccggcc taggcaccac gggcacgctg atgggcactg gccgtttcct gcgcgagcac gttgccaacg tcaagatcgt ggcggccgaa ccccgctacg gtgagggggt atacgccctg cgcaacatgg acgaaggctt tgtgcccgag ctgtatgacc cggaaatact gaccgcgcga tattctgtcg gcgcggtgga cgcagtgcgc cgcacccgcg agttggtgca caccgaaggc atctttgcgg gcatctcaac cggcgcggtg ctacacgccg cactcggagt cggggccggc gccctggcgg ccggcgagcg ggccgacatt gcgttggtgg tcgccgacgc cgggtggaag tatctgtcca ccggcgccta cgccggtagc ctggatgacg ccgagaccgc tctggaaggg caactatggg cataataa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTRYDSLLQ ALGNTPLVGL QRLSPRWDDG RDGPHVRLWA KLEDRNPTGS IKDRPAVRMI EQAEADGLLR PGATILEPTS GNTGISLAMA ARLKGYRLIC VMPENTSVER RQLLELYGAQ IIFSAAEGGS NTAVATAKEL AATNPSWVML YQYGNPANTD SHYCGTGPEL LADLPEITHF VAGLGTTGTL MGTGRFLREH VANVKIVAAE PRYGEGVYAL RNMDEGFVPE LYDPEILTAR YSVGAVDAVR RTRELVHTEG IFAGISTGAV LHAALGVGAG ALAAGERADI ALVVADAGWK YLSTGAYAGS LDDAETALEG QLWA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gacacgatac gactcgctgt tgcaggcctt gggcaacacg ccgctggttg gcctgcagcg attgtcgcca cgctgggatg acgggcgaga cggaccgcac gtgcggctgt gggccaagct cgaggaccgc aatccgaccg ggtcgatcaa ggaccgcccg gctgtgcgga tgatcgagca ggccgaggcc gacgggttgt tgcggccggg cgccaccatc ctggagccca ccagcggaaa caccggcatt tcgctggcga tggcggcccg gttgaagggg taccgattga tctgcgtgat gccggagaac acatcggttg aacggcggca gctgctcgag ctctacggcg cgcagattat cttctcggcg gccgaaggcg ggtccaacac tgcggtggcc accgccaaag agctggccgc gaccaacccg tcatgggtga tgctgtacca gtacggcaat cccgccaaca ccgactcgca ctactgcggc accggccccg agctgctggc cgacctgccc gaaatcacgc acttcgtcgc cggcctaggc accacgggca cgctgatggg cactggccgt ttcctgcgcg agcacgttgc caacgtcaag atcgtggcgg ccgaaccccg ctacggtgag ggggtatacg ccctgcgcaa catggacgaa ggctttgtgc ccgagctgta tgacccggaa atactgaccg cgcgatattc tgtcggcgcg gtggacgcag tgcgccgcac ccgcgagttg gtgcacaccg aaggcatctt tgcgggcatc tcaaccggcg cggtgctaca cgccgcactc ggagtcgggg ccggcgccct ggcggccggc gagcgggccg acattgcgtt ggtggtcgcc gacgccgggt ggaagtatct gtccaccggc gcctacgccg gtagcctgga tgacgccgag accgctctgg aagggcaact atgggcaaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa
Details for MytuD.01113.a.A1.PW26051
PURIFICATION DATe: 2/17/2009
CONCENTRATION: 30.38mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 16
VIAL VOLUME: 100µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTRYDSLLQ ALGNTPLVGL QRLSPRWDDG RDGPHVRLWA KLEDRNPTGS IKDRPAVRMI EQAEADGLLR PGATILEPTS GNTGISLAMA ARLKGYRLIC VMPENTSVER RQLLELYGAQ IIFSAAEGGS NTAVATAKEL AATNPSWVML YQYGNPANTD SHYCGTGPEL LADLPEITHF VAGLGTTGTL MGTGRFLREH VANVKIVAAE PRYGEGVYAL RNMDEGFVPE LYDPEILTAR YSVGAVDAVR RTRELVHTEG IFAGISTGAV LHAALGVGAG ALAAGERADI ALVVADAGWK YLSTGAYAGS LDDAETALEG QLWA
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgacac gatacgactc gctgttgcag gccttgggca acacgccgct ggttggcctg cagcgattgt cgccacgctg ggatgacggg cgagacggac cgcacgtgcg gctgtgggcc aagctcgagg accgcaatcc gaccgggtcg atcaaggacc gcccggctgt gcggatgatc gagcaggccg aggccgacgg gttgttgcgg ccgggcgcca ccatcctgga gcccaccagc ggaaacaccg gcatttcgct ggcgatggcg gcccggttga aggggtaccg attgatctgc gtgatgccgg agaacacatc ggttgaacgg cggcagctgc tcgagctcta cggcgcgcag attatcttct cggcggccga aggcgggtcc aacactgcgg tggccaccgc caaagagctg gccgcgacca acccgtcatg ggtgatgctg taccagtacg gcaatcccgc caacaccgac tcgcactact gcggcaccgg ccccgagctg ctggccgacc tgcccgaaat cacgcacttc gtcgccggcc taggcaccac gggcacgctg atgggcactg gccgtttcct gcgcgagcac gttgccaacg tcaagatcgt ggcggccgaa ccccgctacg gtgagggggt atacgccctg cgcaacatgg acgaaggctt tgtgcccgag ctgtatgacc cggaaatact gaccgcgcga tattctgtcg gcgcggtgga cgcagtgcgc cgcacccgcg agttggtgca caccgaaggc atctttgcgg gcatctcaac cggcgcggtg ctacacgccg cactcggagt cggggccggc gccctggcgg ccggcgagcg ggccgacatt gcgttggtgg tcgccgacgc cgggtggaag tatctgtcca ccggcgccta cgccggtagc ctggatgacg ccgagaccgc tctggaaggg caactatggg cataataa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTRYDSLLQ ALGNTPLVGL QRLSPRWDDG RDGPHVRLWA KLEDRNPTGS IKDRPAVRMI EQAEADGLLR PGATILEPTS GNTGISLAMA ARLKGYRLIC VMPENTSVER RQLLELYGAQ IIFSAAEGGS NTAVATAKEL AATNPSWVML YQYGNPANTD SHYCGTGPEL LADLPEITHF VAGLGTTGTL MGTGRFLREH VANVKIVAAE PRYGEGVYAL RNMDEGFVPE LYDPEILTAR YSVGAVDAVR RTRELVHTEG IFAGISTGAV LHAALGVGAG ALAAGERADI ALVVADAGWK YLSTGAYAGS LDDAETALEG QLWA
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GACACGATAC GACTCGCTGT TGCAGGCCTT GGGCAACACG CCGCTGGTTG GCCTGCAGCG ATTGTCGCCA CGCTGGGATG ACGGGCGAGA CGGACCGCAC GTGCGGCTGT GGGCCAAGCT CGAGGACCGC AATCCGACCG GGTCGATCAA GGACCGCCCG GCTGTGCGGA TGATCGAGCA GGCCGAGGCC GACGGGTTGT TGCGGCCGGG CGCCACCATC CTGGAGCCCA CCAGCGGAAA CACCGGCATT TCGCTGGCGA TGGCGGCCCG GTTGAAGGGG TACCGATTGA TCTGCGTGAT GCCGGAGAAC ACATCGGTTG AACGGCGGCA GCTGCTCGAG CTCTACGGCG CGCAGATTAT CTTCTCGGCG GCCGAAGGCG GGTCCAACAC TGCGGTGGCC ACCGCCAAAG AGCTGGCCGC GACCAACCCG TCATGGGTGA TGCTGTACCA GTACGGCAAT CCCGCCAACA CCGACTCGCA CTACTGCGGC ACCGGCCCCG AGCTGCTGGC CGACCTGCCC GAAATCACGC ACTTCGTCGC CGGCCTAGGC ACCACGGGCA CGCTGATGGG CACTGGCCGT TTCCTGCGCG AGCACGTTGC CAACGTCAAG ATCGTGGCGG CCGAACCCCG CTACGGTGAG GGGGTATACG CCCTGCGCAA CATGGACGAA GGCTTTGTGC CCGAGCTGTA TGACCCGGAA ATACTGACCG CGCGATATTC TGTCGGCGCG GTGGACGCAG TGCGCCGCAC CCGCGAGTTG GTGCACACCG AAGGCATCTT TGCGGGCATC TCAACCGGCG CGGTGCTACA CGCCGCACTC GGAGTCGGGG CCGGCGCCCT GGCGGCCGGC GAGCGGGCCG ACATTGCGTT GGTGGTCGCC GACGCCGGGT GGAAGTATCT GTCCACCGGC GCCTACGCCG GTAGCCTGGA TGACGCCGAG ACCGCTCTGG AAGGGCAACT ATGGGCAAAA CAGCACGAAC AAGTTCTGCA GCCAAGCTTC TCGAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAA