SSGCID: Target Page

MyabA.01113.a

Cysteine synthase

CENTER ID:	MyabA.01113.a
ORGANISM:	Mycobacterium abscessus ATCC 19977 / DSM 44196
ASSOCIATED DISEASE:	Skin abscess
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyabA.01113.a.A1.GE30371	Full length( MyabA.01113.a )		1	324

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyabA.01113.a.A1.PW30256	Full length( MyabA.01113.a )		1	324

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|36809.5.peg.1517
RefSeq:	YP_001702220.1
UniProt:	B1MLY9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTRYDSLLQA LGNTPLVGLQ RLSPRWDSGG DGSPHVRLWA KLEDRNPTGS IKDRPALRMI EQAEQDGLLE RGATILEPTS GNTGISMAMA AQLKGYKMIC VMPENTSIER RQLLELYGAQ IIFSPAEGGS NTAVATAKEL AAQNPSWVML YQYGNPANAD AHYRGTGPEI LADLPEITHF VAGLGTTGTL MGTGRYLREN VPGVQIVAAE PRYGEGVYAL RNIDEGFVPE LYDPEVLTTR FSVGSFDAVR RTRELVQVEG IFAGISTGAI LHAALGMAAK AVKAGERADI AFVVADAGWK YLSTGAYAGT VDDAEDALEG QLWA

NT Sequence

ATGACGCGAT ACGACTCGCT GCTGCAGGCG CTTGGGAACA CGCCACTGGT CGGATTGCAG AGGCTGTCCC CACGGTGGGA TTCAGGTGGT GACGGTTCTC CGCACGTGCG GTTGTGGGCC AAGCTGGAAG ACCGCAATCC GACCGGATCC ATCAAGGACA GGCCCGCGTT GCGCATGATC GAGCAGGCCG AACAGGACGG ATTGCTGGAG CGGGGAGCCA CGATTCTGGA GCCCACCAGT GGGAATACCG GCATTTCGAT GGCGATGGCC GCGCAGCTCA AGGGTTACAA GATGATCTGC GTGATGCCCG AGAACACCTC CATCGAACGG CGCCAGCTCC TCGAACTCTA CGGGGCCCAA ATCATTTTCT CCCCGGCCGA GGGCGGCTCC AACACCGCCG TCGCGACAGC GAAAGAACTT GCCGCGCAAA ATCCTTCGTG GGTGATGCTG TACCAGTACG GCAATCCGGC CAACGCCGAT GCGCACTATC GTGGCACCGG TCCGGAGATT CTGGCCGATC TGCCGGAGAT CACCCACTTC GTCGCCGGAT TGGGCACCAC CGGAACCCTG ATGGGCACCG GAAGATACCT GCGCGAGAAC GTGCCAGGAG TCCAGATCGT CGCCGCCGAA CCGCGATACG GCGAGGGTGT TTACGCACTG CGCAACATCG ATGAGGGATT CGTACCCGAG CTGTACGACC CCGAGGTGCT CACCACGCGC TTCTCCGTCG GGTCCTTCGA CGCGGTGCGC CGCACGCGCG AACTGGTCCA GGTGGAGGGC ATCTTCGCGG GCATCTCAAC CGGCGCGATT CTGCATGCGG CGCTCGGCAT GGCCGCCAAG GCGGTCAAAG CCGGGGAGCG CGCGGACATC GCGTTCGTGG TGGCCGATGC GGGCTGGAAG TATCTGTCGA CCGGTGCCTA CGCCGGTACC GTGGACGACG CAGAGGATGC ACTGGAAGGG CAGCTGTGGG CG

Details for MyabA.01113.a.A1.GE30371

HARVESTED ON:	9/8/2010
SEQUENCED ON:	9/17/2010
EXPECTED MW:	37kDa
OBSERVED MW:	36kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Validated AA Sequence

MGTLEAQTQG PGSMTRYDSL LQALGNTPLV GLQRLSPRWD SGGDGSPHVR LWAKLEDRNP TGSIKDRPAL RMIEQAEQDG LLERGATILE PTSGNTGISM AMAAQLKGYK MICVMPENTS IERRQLLELY GAQIIFSPAE GGSNTAVATA KELAAQNPSW VMLYQYGNPA NADAHYRGTG PEILADLPEI THFVAGLGTT GTLMGTGRYL RENVPGVQIV AAEPRYGEGV YALRNIDEGF VPELYDPEVL TTRFSVGSFD AVRRTRELVQ VEGIFAGIST GAILHAALGM AAKAVKAGER ADIAFVVADA GWKYLSTGAY AGTVDDAEDA LEGQLWA

Validated NT Sequence

ttattacgcc cacagctgcc cttccagtgc atcctctgcg tcgtccacgg taccggcgta ggcaccggtc gacagatact tccagcccgc atcggccacc acgaacgcga tgtccgcgcg ctccccggct ttgaccgcct tggcggccat gccgagcgcc gcatgcagaa tcgcgccggt tgagatgccc gcgaagatgc cctccacctg gaccagttcg cgcgtgcggc gcaccgcgtc gaaggacccg acggagaagc gcgtggtgag cacctcgggg tcgtacagct cgggtacgaa tccctcatcg atgttgcgca gtgcgtaaac accctcgccg tatcgcggtt cggcggcgac gatctggact cctggcacgt tctcgcgcag gtatcttccg gtgcccatca gggttccggt ggtgcccaat ccggcgacga agtgggtgat ctccggcaga tcggccagaa tctccggacc ggtgccacga tagtgcgcat cggcgttggc cggattgccg tactggtaca gcatcaccca cgaaggattt tgcgcggcaa gttctttcgc tgtcgcgacg gcggtgttgg agccgccctc ggccggggag aaaatgattt gggccccgta gagttcgagg agctggcgcc gttcgatgga ggtgttctcg ggcatcacgc agatcatctt gtaacccttg agctgcgcgg ccatcgccat cgaaatgccg gtattcccac tggtgggctc cagaatcgtg gctccccgct ccagcaatcc gtcctgttcg gcctgctcga tcatgcgcaa cgcgggcctg tccttgatgg atccggtcgg attgcggtct tccagcttgg cccacaaccg cacgtgcgga gaaccgtcac cacctgaatc ccaccgtggg gacagcctct gcaatccgac cagtggcgtg ttcccaagcg cctgcagcag cgagtcgtat cgcgtcatcg aaccaggacc ctgggtctga gcttccaggg tacccatatg atggtgatgg tgatga

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMTRYDSLLQ ALGNTPLVGL QRLSPRWDSG GDGSPHVRLW AKLEDRNPTG SIKDRPALRM IEQAEQDGLL ERGATILEPT SGNTGISMAM AAQLKGYKMI CVMPENTSIE RRQLLELYGA QIIFSPAEGG SNTAVATAKE LAAQNPSWVM LYQYGNPANA DAHYRGTGPE ILADLPEITH FVAGLGTTGT LMGTGRYLRE NVPGVQIVAA EPRYGEGVYA LRNIDEGFVP ELYDPEVLTT RFSVGSFDAV RRTRELVQVE GIFAGISTGA ILHAALGMAA KAVKAGERAD IAFVVADAGW KYLSTGAYAG TVDDAEDALE GQLWA

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gacgcgatac gactcgctgc tgcaggcgct tgggaacacg ccactggtcg gattgcagag gctgtcccca cggtgggatt caggtggtga cggttctccg cacgtgcggt tgtgggccaa gctggaagac cgcaatccga ccggatccat caaggacagg cccgcgttgc gcatgatcga gcaggccgaa caggacggat tgctggagcg gggagccacg attctggagc ccaccagtgg gaataccggc atttcgatgg cgatggccgc gcagctcaag ggttacaaga tgatctgcgt gatgcccgag aacacctcca tcgaacggcg ccagctcctc gaactctacg gggcccaaat cattttctcc ccggccgagg gcggctccaa caccgccgtc gcgacagcga aagaacttgc cgcgcaaaat ccttcgtggg tgatgctgta ccagtacggc aatccggcca acgccgatgc gcactatcgt ggcaccggtc cggagattct ggccgatctg ccggagatca cccacttcgt cgccggattg ggcaccaccg gaaccctgat gggcaccgga agatacctgc gcgagaacgt gccaggagtc cagatcgtcg ccgccgaacc gcgatacggc gagggtgttt acgcactgcg caacatcgat gagggattcg tacccgagct gtacgacccc gaggtgctca ccacgcgctt ctccgtcggg tccttcgacg cggtgcgccg cacgcgcgaa ctggtccagg tggagggcat cttcgcgggc atctcaaccg gcgcgattct gcatgcggcg ctcggcatgg ccgccaaggc ggtcaaagcc ggggagcgcg cggacatcgc gttcgtggtg gccgatgcgg gctggaagta tctgtcgacc ggtgcctacg ccggtaccgt ggacgacgca gaggatgcac tggaagggca gctgtgggcg taaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa

Details for MyabA.01113.a.A1.PW30256

PURIFICATION DATe:	12/6/2010
CONCENTRATION:	27.46mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	10
VIAL VOLUME:	200µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GACGCGATAC GACTCGCTGC TGCAGGCGCT TGGGAACACG CCACTGGTCG GATTGCAGAG GCTGTCCCCA CGGTGGGATT CAGGTGGTGA CGGTTCTCCG CACGTGCGGT TGTGGGCCAA GCTGGAAGAC CGCAATCCGA CCGGATCCAT CAAGGACAGG CCCGCGTTGC GCATGATCGA GCAGGCCGAA CAGGACGGAT TGCTGGAGCG GGGAGCCACG ATTCTGGAGC CCACCAGTGG GAATACCGGC ATTTCGATGG CGATGGCCGC GCAGCTCAAG GGTTACAAGA TGATCTGCGT GATGCCCGAG AACACCTCCA TCGAACGGCG CCAGCTCCTC GAACTCTACG GGGCCCAAAT CATTTTCTCC CCGGCCGAGG GCGGCTCCAA CACCGCCGTC GCGACAGCGA AAGAACTTGC CGCGCAAAAT CCTTCGTGGG TGATGCTGTA CCAGTACGGC AATCCGGCCA ACGCCGATGC GCACTATCGT GGCACCGGTC CGGAGATTCT GGCCGATCTG CCGGAGATCA CCCACTTCGT CGCCGGATTG GGCACCACCG GAACCCTGAT GGGCACCGGA AGATACCTGC GCGAGAACGT GCCAGGAGTC CAGATCGTCG CCGCCGAACC GCGATACGGC GAGGGTGTTT ACGCACTGCG CAACATCGAT GAGGGATTCG TACCCGAGCT GTACGACCCC GAGGTGCTCA CCACGCGCTT CTCCGTCGGG TCCTTCGACG CGGTGCGCCG CACGCGCGAA CTGGTCCAGG TGGAGGGCAT CTTCGCGGGC ATCTCAACCG GCGCGATTCT GCATGCGGCG CTCGGCATGG CCGCCAAGGC GGTCAAAGCC GGGGAGCGCG CGGACATCGC GTTCGTGGTG GCCGATGCGG GCTGGAAGTA TCTGTCGACC GGTGCCTACG CCGGTACCGT GGACGACGCA GAGGATGCAC TGGAAGGGCA GCTGTGGGCG TAAACAGCAC GAACAAGTTC TGCAGCCAAG CTTCTCGAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAA