SSGCID: Target Page

MythA.01113.a

Cysteine synthase (A0R1X2 homolog)

CENTER ID:	MythA.01113.a
ORGANISM:	Mycobacterium thermoresistibile ATCC 19527 / NCTC 10409
ASSOCIATED DISEASE:	Respiratory Infection
CURRENT STATUS:	crystallized
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

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Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MythA.01113.a.A1.GE28768	Full length( MythA.01113.a )		1	320

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MythA.01113.a.A1.PW28670	Full length( MythA.01113.a )		1	320

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|1078020.3.peg.2556
RefSeq:	WP_003926082.1
UniProt:	G7CL11

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTRYNSLLES LGNTPLVGLQ RLSPRWDGEP HVRLWAKLED RNPTGSIKDR AALRMIEDAE RQGLLSPGAT ILEPTSGNTG ISLAMAARLK GYRLICVMPE NTSVERRQLL ELFGAEIIYS PAEGGSNTAV ARAKELAAQN PSWVMLYQYG NPANAAAHHD GTGPELLADL PEITHFVAGL GTTGTLMGTG RFLRERVPGV QIVAAEPRYG EGVYALRNID EGFIPELYDP DVLTTRYSVG SFDAIRRTRE LVQTEGIFAG ISTGAVLHAA LAMAAKAVTA QQRADIAFVV ADAGWKYLST GAYTGSLDEA EDALEGQLWA

NT Sequence

GTGACCCGCT ACAACTCGCT GCTCGAATCG CTCGGCAACA CCCCGCTGGT CGGGCTGCAG CGGCTGTCGC CCCGCTGGGA CGGCGAACCG CACGTGCGGT TGTGGGCCAA GCTGGAGGAC CGCAACCCGA CCGGATCGAT CAAGGACCGG GCCGCGCTGC GGATGATCGA GGACGCCGAA CGGCAGGGCC TGCTGTCGCC CGGGGCGACG ATCCTCGAGC CCACCAGCGG CAACACCGGC ATCTCGCTGG CGATGGCCGC CCGGCTCAAG GGCTACCGGT TGATCTGCGT GATGCCGGAG AACACCTCGG TGGAACGTCG CCAACTGCTG GAACTGTTCG GCGCCGAGAT CATCTACTCA CCGGCCGAGG GCGGCTCGAA CACCGCCGTC GCCCGGGCCA AGGAACTGGC CGCGCAGAAC CCGTCGTGGG TGATGCTGTA TCAGTACGGC AACCCCGCCA ACGCCGCTGC GCACCATGAC GGCACCGGCC CGGAACTGCT GGCCGACCTG CCCGAGATCA CCCATTTCGT GGCCGGTCTG GGCACCACCG GAACCCTGAT GGGCACCGGG CGGTTCCTGC GCGAACGCGT CCCCGGCGTG CAGATCGTCG CGGCCGAACC CCGCTACGGC GAAGGGGTGT ACGCGCTGCG CAACATCGAC GAGGGCTTCA TCCCGGAGCT CTACGACCCG GACGTGCTGA CCACCCGCTA CTCGGTCGGC TCGTTCGACG CGATCCGCCG CACCCGCGAG CTGGTGCAGA CCGAGGGCAT CTTCGCCGGG ATCTCGACCG GCGCGGTGCT GCACGCCGCG CTGGCGATGG CGGCGAAGGC GGTCACGGCG CAGCAACGTG CCGACATCGC GTTCGTGGTG GCCGACGCCG GGTGGAAGTA TCTGTCCACC GGTGCCTACA CCGGTAGCCT GGATGAGGCC GAAGACGCGC TGGAAGGGCA GCTATGGGCA

Details for MythA.01113.a.A1.GE28768

HARVESTED ON:	3/1/2010
SEQUENCED ON:	5/19/2010
EXPECTED MW:	36kDa
OBSERVED MW:	36kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Few (1-10)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMVTRYNSLL ESLGNTPLVG LQRLSPRWDG EPHVRLWAKL EDRNPTGSIK DRAALRMIED AERQGLLSPG ATILEPTSGN TGISLAMAAR LKGYRLICVM PENTSVERRQ LLELFGAEII YSPAEGGSNT AVARAKELAA QNPSWVMLYQ YGNPANAAAH HDGTGPELLA DLPEITHFVA GLGTTGTLMG TGRFLRERVP GVQIVAAEPR YGEGVYALRN IDEGFIPELY DPDVLTTRYS VGSFDAIRRT RELVQTEGIF AGISTGAVLH AALAMAAKAV TAQQRADIAF VVADAGWKYL STGAYTGSLD EAEDALEGQL WA

Validated NT Sequence

atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggtga cccgctacaa ctcgctgctc gaatcgctcg gcaacacccc gctggtcggg ctgcagcggc tgtcgccccg ctgggacggc gaaccgcacg tgcggttgtg ggccaagctg gaggaccgca acccgaccgg atcgatcaag gaccgggccg cgctgcggat gatcgaggac gccgaacggc agggcctgct gtcgcccggg gcgacgatcc tcgagcccac cagcggcaac accggcatct cgctggcgat ggccgcccgg ctcaagggct accggttgat ctgcgtgatg ccggagaaca cctcggtgga acgtcgccaa ctgctggaac tgttcggcgc cgagatcatc tactcaccgg ccgagggcgg ctcgaacacc gccgtcgccc gggccaagga actggccgcg cagaacccgt cgtgggtgat gctgtatcag tacggcaacc ccgccaacgc cgctgcgcac catgacggca ccggcccgga actgctggcc gacctgcccg agatcaccca tttcgtggcc ggtctgggca ccaccggaac cctgatgggc accgggcggt tcctgcgcga acgcgtcccc ggcgtgcaga tcgtcgcggc cgaaccccgc tacggcgaag gggtgtacgc gctgcgcaac atcgacgagg gcttcatccc ggagctctac gacccggacg tgctgaccac ccgctactcg gtcggctcgt tcgacgcgat ccgccgcacc cgcgagctgg tgcagaccga gggcatcttc gccgggatct cgaccggcgc ggtgctgcac gccgcgctgg cgatggcggc gaaggcggtc acggcgcagc aacgtgccga catcgcgttc gtggtggccg acgccgggtg gaagtatctg tccaccggtg cctacaccgg tagcctggat gaggccgaag acgcgctgga agggcagcta tgggcataat aa

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgacccgc tacaactcgc tgctcgaatc gctcggcaac accccgctgg tcgggctgca gcggctgtcg ccccgctggg acggcgaacc gcacgtgcgg ttgtgggcca agctggagga ccgcaacccg accggatcga tcaaggaccg ggccgcgctg cggatgatcg aggacgccga acggcagggc ctgctgtcgc ccggggcgac gatcctcgag cccaccagcg gcaacaccgg catctcgctg gcgatggccg cccggctcaa gggctaccgg ttgatctgcg tgatgccgga gaacacctcg gtggaacgtc gccaactgct ggaactgttc ggcgccgaga tcatctactc accggccgag ggcggctcga acaccgccgt cgcccgggcc aaggaactgg ccgcgcagaa cccgtcgtgg gtgatgctgt atcagtacgg caaccccgcc aacgccgctg cgcaccatga cggcaccggc ccggaactgc tggccgacct gcccgagatc acccatttcg tggccggtct gggcaccacc ggaaccctga tgggcaccgg gcggttcctg cgcgaacgcg tccccggcgt gcagatcgtc gcggccgaac cccgctacgg cgaaggggtg tacgcgctgc gcaacatcga cgagggcttc atcccggagc tctacgaccc ggacgtgctg accacccgct actcggtcgg ctcgttcgac gcgatccgcc gcacccgcga gctggtgcag accgagggca tcttcgccgg gatctcgacc ggcgcggtgc tgcacgccgc gctggcgatg gcggcgaagg cggtcacggc gcagcaacgt gccgacatcg cgttcgtggt ggccgacgcc gggtggaagt atctgtccac cggtgcctac accggtagcc tggatgaggc cgaagacgcg ctggaagggc agctatgggc aaaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa

Details for MythA.01113.a.A1.PW28670

PURIFICATION DATe:	6/14/2010
CONCENTRATION:	27.85mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	5
VIAL VOLUME:	200µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGACCCGC TACAACTCGC TGCTCGAATC GCTCGGCAAC ACCCCGCTGG TCGGGCTGCA GCGGCTGTCG CCCCGCTGGG ACGGCGAACC GCACGTGCGG TTGTGGGCCA AGCTGGAGGA CCGCAACCCG ACCGGATCGA TCAAGGACCG GGCCGCGCTG CGGATGATCG AGGACGCCGA ACGGCAGGGC CTGCTGTCGC CCGGGGCGAC GATCCTCGAG CCCACCAGCG GCAACACCGG CATCTCGCTG GCGATGGCCG CCCGGCTCAA GGGCTACCGG TTGATCTGCG TGATGCCGGA GAACACCTCG GTGGAACGTC GCCAACTGCT GGAACTGTTC GGCGCCGAGA TCATCTACTC ACCGGCCGAG GGCGGCTCGA ACACCGCCGT CGCCCGGGCC AAGGAACTGG CCGCGCAGAA CCCGTCGTGG GTGATGCTGT ATCAGTACGG CAACCCCGCC AACGCCGCTG CGCACCATGA CGGCACCGGC CCGGAACTGC TGGCCGACCT GCCCGAGATC ACCCATTTCG TGGCCGGTCT GGGCACCACC GGAACCCTGA TGGGCACCGG GCGGTTCCTG CGCGAACGCG TCCCCGGCGT GCAGATCGTC GCGGCCGAAC CCCGCTACGG CGAAGGGGTG TACGCGCTGC GCAACATCGA CGAGGGCTTC ATCCCGGAGC TCTACGACCC GGACGTGCTG ACCACCCGCT ACTCGGTCGG CTCGTTCGAC GCGATCCGCC GCACCCGCGA GCTGGTGCAG ACCGAGGGCA TCTTCGCCGG GATCTCGACC GGCGCGGTGC TGCACGCCGC GCTGGCGATG GCGGCGAAGG CGGTCACGGC GCAGCAACGT GCCGACATCG CGTTCGTGGT GGCCGACGCC GGGTGGAAGT ATCTGTCCAC CGGTGCCTAC ACCGGTAGCC TGGATGAGGC CGAAGACGCG CTGGAAGGGC AGCTATGGGC AAAACAGCAC GAACAAGTTC TGCAGCCAAG CTTCTCGAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAA