MyulA.01113.a

Cysteine synthase

CENTER ID: MyulA.01113.a
ORGANISM: Mycobacterium ulcerans Agy99
ASSOCIATED DISEASE: Buruli, Bairnsdale ulcer
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyulA.01113.a.A1.GE29770 Full length( MyulA.01113.a ) 1 314
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|362242.7.peg.361
RefSeq: YP_904455.1
UniProt: A0PKW5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTRYDSLLAT LGDTPLVGLP RLSPGWDNSP HVRLWAKLED RNPTGSIKDR PALRMIEQAE ADGWLRPGAT ILEATSGNTG ISLAMAAKLK GYRLICVIPE NTSIERRRLL ELYGTEIIFS PAEGGSNTAV ALAKQLAAEH PEWVLLYQYG NPANSESHYR GTGPELLADL PEITHFVAGL GSSGTLMGAG RFLREHVANI KIVAVEPRHN EKLCGMRNLD EGFVPELYDP TLGAVRCPVA TADAEHRIRE LAHTEGVLAG LSTGAALHAA LEIGTQALRA GERADIAFVV ADAAWKYLSS GVYEHPARSK LLAP
NT Sequence
ATGACCCGCT ACGACTCACT GCTGGCCACC TTGGGCGATA CGCCCCTGGT TGGCTTACCA CGACTCTCAC CAGGCTGGGA CAACAGTCCG CACGTGCGGT TGTGGGCCAA ACTCGAAGAC CGCAACCCCA CCGGGTCGAT CAAAGATCGC CCAGCGCTGC GGATGATCGA GCAAGCCGAG GCCGACGGTT GGCTGCGGCC GGGCGCGACG ATCCTGGAGG CCACCAGCGG CAACACCGGC ATCTCCCTGG CGATGGCCGC CAAACTGAAG GGGTATCGGC TCATCTGCGT GATACCGGAA AACACCTCGA TCGAGCGCCG TCGGTTGCTC GAGCTCTACG GGACCGAGAT CATATTTTCC CCCGCCGAGG GCGGATCCAA CACCGCGGTA GCGCTCGCCA AACAACTGGC CGCCGAACAC CCCGAATGGG TCCTGCTCTA CCAGTACGGC AACCCCGCCA ACTCCGAGTC CCACTACCGC GGCACCGGCC CGGAACTGCT CGCCGACCTG CCCGAGATCA CCCACTTCGT CGCCGGCCTG GGCTCCTCGG GCACTCTGAT GGGCGCCGGC CGATTCCTGC GCGAACACGT CGCCAACATC AAGATCGTGG CCGTCGAACC CCGCCACAAC GAAAAACTCT GTGGGATGCG CAATCTCGAC GAAGGATTTG TGCCCGAACT GTATGACCCC ACGCTGGGGG CCGTGCGCTG CCCGGTCGCC ACCGCCGATG CCGAGCACCG CATCCGCGAA CTCGCACACA CCGAGGGCGT CCTGGCAGGC CTGTCCACGG GCGCGGCCCT ACACGCCGCA TTGGAGATCG GCACTCAAGC TCTGCGAGCA GGCGAGCGTG CCGATATCGC GTTCGTCGTC GCCGACGCCG CGTGGAAATA CCTCTCCTCC GGCGTCTATG AACACCCGGC ACGTAGCAAG TTGCTGGCCC CA
Details for MyulA.01113.a.A1.GE29770
HARVESTED ON: 6/29/2010
SEQUENCED ON: 7/7/2010
EXPECTED MW: 36kDa
OBSERVED MW: 35kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 88
PERCENT COVERAGE: 110
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTRYDSLLA TLGDTPLVGL PRLSPGWDNS PHVRLWAKLE DRNPTGSIKD RPALRMIEQA EADGWLRPGA TILEATSGNT GISLAMAAKL KGYRLICVIP ENTSIERRRL LELYGTEIIF SPAEGGSNTA VALAKQLAAE HPEWVLLYQY GNPANSESHY RGTGPELLAD LPEITHFVAG LGSSGTLMGA GRFLREHVAN IKIVAVEPRH NEKLCGMRNL DEXFVPELYX PTLGAVRCPV ATADAEHRIR ELAHTEGVXX XLSTGAXLHA ALXXGTQALX AXXRXXXAFX XXXXXXXXLS XXXXXXXXVX XAGXXXKXXX XXXXXXLXXX XXXXXXXXXX XXXXXXXXXX X
Validated NT Sequence
ttgtttantt taagaaggag atataccatg gctcatcacc atcaccatca tatgggtacc ctggaagctc agacccaggg tcctggttcg atgacccgct acgactcact gctggccacc ttgggcgata cgcccctggt tggcttacca cgactctcac caggctggga caacagtccg cacgtgcggt tgtgggccaa actcgaagac cgcaacccca ccgggtcgat caaagatcgc ccagcgctgc ggatgatcga gcaagccgag gccgacggtt ggctgcggcc gggcgcgacg atcctggagg ccaccagcgg caacaccggc atctccctgg cgatggccgc caaactgaag gggtatcggc tcatctgcgt gataccggaa aacacctcga tcgagcgccg tcggttgctc gagctctacg ggaccgagat catattttcc cccgccgagg gcggatccaa caccgcggta gcgctcgcca aacaactggc cgccgaacac cccgaatggg tcctgctcta ccagtacggc aaccccgcca actccgagtc ccactaccgc ggcaccggcc cggaactgct cgccgacctg cccgagatca cccacttcgt cgccggcctg ggctcctcgg gcactctgat gggcgccggc cgattcctgc gcgaacacgt cgccaacatc aagatcgtgg ccgtcgaacc ccgccacaac gaaaaactct gtgggatgcg caatctcgac gaangatttg tgcccgaact gtatganccc acgctggggg ccgtgcgctg cccggtcgcc accgccgatg ccgagcaccg catccgcgaa ctcgcacaca ccgagggcgt cnnnnnnngc ctgtccacgg gngcggnnct ncacgccgca ttgnnnancg gcactcaagc tctgcnagca ngnnancgng nnnanntcgc gttcgncgnc nnccnanncn ncnnnnaann cctctcctnc nnngnnnntg nnnccnnnnn cgtancannn gctggnnccc nnnataaann nncncnannn nnnnnnnnan nnnanctnnn nnnnnnnnnn nnnnnnnncc naaangnann tnnnnngnnn nnnnncnnnn nnnnnnnnnn nannnnnnnn a
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTRYDSLLA TLGDTPLVGL PRLSPGWDNS PHVRLWAKLE DRNPTGSIKD RPALRMIEQA EADGWLRPGA TILEATSGNT GISLAMAAKL KGYRLICVIP ENTSIERRRL LELYGTEIIF SPAEGGSNTA VALAKQLAAE HPEWVLLYQY GNPANSESHY RGTGPELLAD LPEITHFVAG LGSSGTLMGA GRFLREHVAN IKIVAVEPRH NEKLCGMRNL DEGFVPELYD PTLGAVRCPV ATADAEHRIR ELAHTEGVLA GLSTGAALHA ALEIGTQALR AGERADIAFV VADAAWKYLS SGVYEHPARS KLLAP
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gacccgctac gactcactgc tggccacctt gggcgatacg cccctggttg gcttaccacg actctcacca ggctgggaca acagtccgca cgtgcggttg tgggccaaac tcgaagaccg caaccccacc gggtcgatca aagatcgccc agcgctgcgg atgatcgagc aagccgaggc cgacggttgg ctgcggccgg gcgcgacgat cctggaggcc accagcggca acaccggcat ctccctggcg atggccgcca aactgaaggg gtatcggctc atctgcgtga taccggaaaa cacctcgatc gagcgccgtc ggttgctcga gctctacggg accgagatca tattttcccc cgccgagggc ggatccaaca ccgcggtagc gctcgccaaa caactggccg ccgaacaccc cgaatgggtc ctgctctacc agtacggcaa ccccgccaac tccgagtccc actaccgcgg caccggcccg gaactgctcg ccgacctgcc cgagatcacc cacttcgtcg ccggcctggg ctcctcgggc actctgatgg gcgccggccg attcctgcgc gaacacgtcg ccaacatcaa gatcgtggcc gtcgaacccc gccacaacga aaaactctgt gggatgcgca atctcgacga aggatttgtg cccgaactgt atgaccccac gctgggggcc gtgcgctgcc cggtcgccac cgccgatgcc gagcaccgca tccgcgaact cgcacacacc gagggcgtcc tggcaggcct gtccacgggc gcggccctac acgccgcatt ggagatcggc actcaagctc tgcgagcagg cgagcgtgcc gatatcgcgt tcgtcgtcgc cgacgccgcg tggaaatacc tctcctccgg cgtctatgaa cacccggcac gtagcaagtt gctggcccca aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa