MytuD.01073.a

NADH dehydrogenase subunit C

CENTER ID: MytuD.01073.a
ORGANISM: Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE: Tuberculosis
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MytuD.01073.a.A1.GE25928 Full length( MytuD.01073.a ) 1 236
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MytuD.01073.a.A1.PS00199 Full length( MytuD.01073.a ) 1 236

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|83332.12.peg.3513
RefSeq: NP_217663.1
TubercuList: Rv3147
MTB Network Portal: Rv3147
UniProt: P9WJH3

Orthologues for Rv3147

Species % Identity % Coverage Clones Proteins Structures
marinum 86 100 1 1 0
ulcerans 85 100 1 1 0
avium 80 100 1 1 0
paratuberculosis 79 100 1 1 0
smegmatis 73 100 1 1 0
abscessus 72 92 1 0 0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSPPNQDAQE GRPDSPTAEV VDVRRGMFGV SGTGDTSGYG RLVRQVVLPG SSPRPYGGYF DDIVDRLAEA LRHERVEFED AVEKVVVYRD ELTLHVRRDL LPRVAQRLRD EPELRFELCL GVSGVHYPHE TGRELHAVYP LQSITHNRRL RLEVSAPDSD PHIPSLFAIY PTNDWHERET YDFFGIIFDG HPALTRIEMP DDWQGHPQRK DYPLGGIPVE YKGAQIPPPD ERRGYN
NT Sequence
ATGAGCCCGC CGAACCAAGA CGCCCAGGAA GGCCGCCCGG ACTCCCCCAC CGCGGAGGTG GTCGACGTTC GCCGCGGCAT GTTCGGCGTC TCGGGCACCG GTGACACCTC CGGTTACGGA CGGTTGGTGC GCCAAGTCGT CCTCCCTGGC AGCAGCCCCC GGCCCTACGG CGGCTACTTC GACGATATCG TCGACCGGCT GGCCGAGGCA CTGCGGCACG AGCGCGTCGA ATTCGAGGAC GCCGTCGAGA AAGTCGTGGT CTACCGCGAT GAACTGACCC TGCACGTCCG CCGGGATCTA CTGCCGCGGG TCGCCCAGCG GCTGCGCGAC GAACCCGAAT TGCGATTCGA GCTGTGTCTT GGGGTGAGCG GGGTGCACTA CCCGCACGAG ACGGGTCGGG AGCTGCATGC CGTCTACCCG CTGCAGTCGA TCACCCACAA CCGTCGCCTC CGGTTGGAAG TGTCTGCGCC GGACAGTGAT CCGCACATCC CTTCCCTGTT CGCGATCTAT CCGACCAACG ACTGGCACGA GCGGGAAACC TACGACTTCT TCGGGATCAT CTTCGACGGC CATCCGGCCC TGACCCGGAT CGAGATGCCC GATGACTGGC AGGGGCATCC GCAACGCAAG GACTACCCTC TCGGCGGCAT CCCGGTCGAA TACAAGGGCG CGCAGATACC CCCGCCCGAC GAGCGGAGGG GCTACAAC
Details for MytuD.01073.a.A1.GE25928
HARVESTED ON: 1/9/2009
SEQUENCED ON: 8/18/2010
EXPECTED MW: 29kDa
OBSERVED MW: 30kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSPPNQDAQ EGRPDSPTAE VVDVRRGMFG VSGTGDTSGY GRLVRQVVLP GSSPRPYGGY FDDIVDRLAE ALRHERVEFE DAVEKVVVYR DELTLHVRRD LLPRVAQRLR DEPELRFELC LGVSGVHYPH ETGRELHAVY PLQSITHNRR LRLEVSAPDS DPHIPSLFAI YPTNDWHERE TYDFFGIIFD GHPALTRIEM PDDWQGHPQR KDYPLGGIPV EYKGAQIPPP DERRG
Validated NT Sequence
gaaggagata taccatggct catcaccatc accatcatat gggtaccctg gaagctcaga cccagggtcc tggttcgatg agcccgccga accaagacgc ccaggaaggc cgcccggact cccccaccgc ggaggtggtc gacgttcgcc gcggcatgtt cggcgtctcg ggcaccggtg acacctccgg ttacggacgg ttggtgcgcc aagtcgtcct ccctggcagc agcccccggc cctacggcgg ctacttcgac gatatcgtcg accggctggc cgaggcactg cggcacgagc gcgtcgaatt cgaggacgcc gtcgagaaag tcgtggtcta ccgcgatgaa ctgaccctgc acgtccgccg ggatctactg ccgcgggtcg cccagcggct gcgcgacgaa cccgaattgc gattcgagct gtgtcttggg gtgagcgggg tgcactaccc gcacgagacg ggtcgggagc tgcatgccgt ctacccgctg cagtcgatca cccacaaccg tcgcctccgg ttggaagtgt ctgcgccgga cagtgatccg cacatccctt ccctgttcgc gatctatccg accaacgact ggcacgagcg ggaaacctac gacttcttcg ggatcatctt cgacggccat ccggccctga cccggatcga gatgcccgat gactggcagg ggcatccgca acgcaaggac taccctctcg gcggcatccc ggtcgaatac aagggcgcgc agataccccc gcccgacgag cggaggggct
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSPPNQDAQ EGRPDSPTAE VVDVRRGMFG VSGTGDTSGY GRLVRQVVLP GSSPRPYGGY FDDIVDRLAE ALRHERVEFE DAVEKVVVYR DELTLHVRRD LLPRVAQRLR DEPELRFELC LGVSGVHYPH ETGRELHAVY PLQSITHNRR LRLEVSAPDS DPHIPSLFAI YPTNDWHERE TYDFFGIIFD GHPALTRIEM PDDWQGHPQR KDYPLGGIPV EYKGAQIPPP DERRGYN
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagcccgccg aaccaagacg cccaggaagg ccgcccggac tcccccaccg cggaggtggt cgacgttcgc cgcggcatgt tcggcgtctc gggcaccggt gacacctccg gttacggacg gttggtgcgc caagtcgtcc tccctggcag cagcccccgg ccctacggcg gctacttcga cgatatcgtc gaccggctgg ccgaggcact gcggcacgag cgcgtcgaat tcgaggacgc cgtcgagaaa gtcgtggtct accgcgatga actgaccctg cacgtccgcc gggatctact gccgcgggtc gcccagcggc tgcgcgacga acccgaattg cgattcgagc tgtgtcttgg ggtgagcggg gtgcactacc cgcacgagac gggtcgggag ctgcatgccg tctacccgct gcagtcgatc acccacaacc gtcgcctccg gttggaagtg tctgcgccgg acagtgatcc gcacatccct tccctgttcg cgatctatcc gaccaacgac tggcacgagc gggaaaccta cgacttcttc gggatcatct tcgacggcca tccggccctg acccggatcg agatgcccga tgactggcag gggcatccgc aacgcaagga ctaccctctc ggcggcatcc cggtcgaata caagggcgcg cagatacccc cgcccgacga gcggaggggc tacaacaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa
Details for MytuD.01073.a.A1.PS00199
PURIFICATION DATe: 4/15/2009
CONCENTRATION: 25.26mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 10
VIAL VOLUME: 100µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSPPNQDAQ EGRPDSPTAE VVDVRRGMFG VSGTGDTSGY GRLVRQVVLP GSSPRPYGGY FDDIVDRLAE ALRHERVEFE DAVEKVVVYR DELTLHVRRD LLPRVAQRLR DEPELRFELC LGVSGVHYPH ETGRELHAVY PLQSITHNRR LRLEVSAPDS DPHIPSLFAI YPTNDWHERE TYDFFGIIFD GHPALTRIEM PDDWQGHPQR KDYPLGGIPV EYKGAQIPPP DERRG
Validated NT Sequence
gaaggagata taccatggct catcaccatc accatcatat gggtaccctg gaagctcaga cccagggtcc tggttcgatg agcccgccga accaagacgc ccaggaaggc cgcccggact cccccaccgc ggaggtggtc gacgttcgcc gcggcatgtt cggcgtctcg ggcaccggtg acacctccgg ttacggacgg ttggtgcgcc aagtcgtcct ccctggcagc agcccccggc cctacggcgg ctacttcgac gatatcgtcg accggctggc cgaggcactg cggcacgagc gcgtcgaatt cgaggacgcc gtcgagaaag tcgtggtcta ccgcgatgaa ctgaccctgc acgtccgccg ggatctactg ccgcgggtcg cccagcggct gcgcgacgaa cccgaattgc gattcgagct gtgtcttggg gtgagcgggg tgcactaccc gcacgagacg ggtcgggagc tgcatgccgt ctacccgctg cagtcgatca cccacaaccg tcgcctccgg ttggaagtgt ctgcgccgga cagtgatccg cacatccctt ccctgttcgc gatctatccg accaacgact ggcacgagcg ggaaacctac gacttcttcg ggatcatctt cgacggccat ccggccctga cccggatcga gatgcccgat gactggcagg ggcatccgca acgcaaggac taccctctcg gcggcatccc ggtcgaatac aagggcgcgc agataccccc gcccgacgag cggaggggct
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSPPNQDAQ EGRPDSPTAE VVDVRRGMFG VSGTGDTSGY GRLVRQVVLP GSSPRPYGGY FDDIVDRLAE ALRHERVEFE DAVEKVVVYR DELTLHVRRD LLPRVAQRLR DEPELRFELC LGVSGVHYPH ETGRELHAVY PLQSITHNRR LRLEVSAPDS DPHIPSLFAI YPTNDWHERE TYDFFGIIFD GHPALTRIEM PDDWQGHPQR KDYPLGGIPV EYKGAQIPPP DERRGYN
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAGCCCGCCG AACCAAGACG CCCAGGAAGG CCGCCCGGAC TCCCCCACCG CGGAGGTGGT CGACGTTCGC CGCGGCATGT TCGGCGTCTC GGGCACCGGT GACACCTCCG GTTACGGACG GTTGGTGCGC CAAGTCGTCC TCCCTGGCAG CAGCCCCCGG CCCTACGGCG GCTACTTCGA CGATATCGTC GACCGGCTGG CCGAGGCACT GCGGCACGAG CGCGTCGAAT TCGAGGACGC CGTCGAGAAA GTCGTGGTCT ACCGCGATGA ACTGACCCTG CACGTCCGCC GGGATCTACT GCCGCGGGTC GCCCAGCGGC TGCGCGACGA ACCCGAATTG CGATTCGAGC TGTGTCTTGG GGTGAGCGGG GTGCACTACC CGCACGAGAC GGGTCGGGAG CTGCATGCCG TCTACCCGCT GCAGTCGATC ACCCACAACC GTCGCCTCCG GTTGGAAGTG TCTGCGCCGG ACAGTGATCC GCACATCCCT TCCCTGTTCG CGATCTATCC GACCAACGAC TGGCACGAGC GGGAAACCTA CGACTTCTTC GGGATCATCT TCGACGGCCA TCCGGCCCTG ACCCGGATCG AGATGCCCGA TGACTGGCAG GGGCATCCGC AACGCAAGGA CTACCCTCTC GGCGGCATCC CGGTCGAATA CAAGGGCGCG CAGATACCCC CGCCCGACGA GCGGAGGGGC TACAACAAAC AGCACGAACA AGTTCTGCAG CCAAGCTTCT CGAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAA