MymaA.01073.a

NADH-quinone oxidoreductase subunit C

CENTER ID: MymaA.01073.a
ORGANISM: Mycobacterium marinum ATCC BAA-535 / M
ASSOCIATED DISEASE: Cutaneous ulcerations, deep abscesses
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MymaA.01073.a.A1.GE30134 Full length( MymaA.01073.a ) 1 238
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MymaA.01073.a.A1.PW30007 Full length( MymaA.01073.a ) 1 238

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216594.6.peg.1579
RefSeq: YP_001849787.1
UniProt: B2HGE6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSSPDQNPSD AAGQTGSSNE EVVDVRRGMF GVKGTGDTSG YGRLVREIVL PGSSPRPYGF YFDEIADRLA EALNRDGVEF EDAIEKVVVY RNELTLHVRR EALLRVAQSL RDEPELRFEL CLGVNGVHYP HETGRELHAV YPLQSITHNR RLRLEVSAPD SDPHIPSLYA VYPTNDWHER ETYDFFGIIF DGHPSLTRIE MPDDWQGHPQ RKDYPLGGIP VEYKGAQIPP PDERRGYN
NT Sequence
ATGAGCTCGC CAGACCAGAA CCCATCAGAC GCAGCGGGCC AAACCGGCTC CTCCAATGAA GAAGTGGTCG ACGTGCGTCG CGGCATGTTC GGCGTCAAAG GCACCGGCGA CACCTCCGGA TACGGGCGAT TGGTGCGCGA GATCGTGCTG CCGGGCAGTA GCCCCCGCCC GTACGGCTTC TACTTCGACG AGATCGCCGA TCGCCTGGCC GAGGCGCTGA ACCGCGACGG CGTCGAATTC GAGGATGCCA TAGAGAAAGT CGTGGTCTAT CGCAACGAGT TGACCCTGCA CGTTCGCCGC GAGGCCCTAC TGCGAGTCGC ACAGTCCCTC CGCGACGAGC CGGAATTGCG CTTTGAGCTG TGCCTGGGAG TCAACGGGGT GCACTACCCG CATGAGACCG GCCGCGAACT ACACGCGGTT TACCCGCTGC AATCGATCAC CCACAACCGT CGCCTGCGGC TGGAAGTGTC TGCCCCCGAC AGCGATCCGC ATATCCCGTC GCTATACGCC GTCTACCCGA CCAACGATTG GCACGAGCGC GAGACATACG ACTTCTTCGG CATCATCTTC GACGGGCATC CCTCGCTGAC GCGAATCGAG ATGCCTGACG ACTGGCAGGG GCACCCACAG CGCAAGGACT ATCCACTCGG CGGCATCCCG GTCGAATACA AGGGCGCGCA GATACCCCCG CCCGACGAGC GAAGGGGTTA CAAC
Details for MymaA.01073.a.A1.GE30134
HARVESTED ON: 8/15/2010
SEQUENCED ON: 8/25/2010
EXPECTED MW: 29kDa
OBSERVED MW: 34kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSSPDQNPS DAAGQTGSSN EEVVDVRRGM FGVKGTGDTS GYGRLVREIV LPGSSPRPYG FYFDEIADRL AEALNRDGVE FEDAIEKVVV YRNELTLHVR REALLRVAQS LRDEPELRFE LCLGVNGVHY PHETGRELHA VYPLQSITHN RRLRLEVSAP DSDPHIPSLY AVYPTNDWHE RETYDFFGII FDGHPSLTRI EMPDDWQGHP QRKDYPLGGI PVEYKGAQIP PPDERRG
Validated NT Sequence
aaccccttcg ctcgtcgggc gggggtatct gcgcgccctt gtattcgacc gggatgccgc cgagtggata gtccttgcgc tgtgggtgcc cctgccagtc gtcaggcatc tcgattcgcg tcagcgaggg atgcccgtcg aagatgatgc cgaagaagtc gtatgtctcg cgctcgtgcc aatcgttggt cgggtagacg gcgtatagcg acgggatatg cggatcgctg tcgggggcag acacttccag ccgcaggcga cggttgtggg tgatcgattg cagcgggtaa accgcgtgta gttcgcggcc ggtctcatgc gggtagtgca ccccgttgac tcccaggcac agctcaaagc gcaattccgg ctcgtcgcgg agggactgtg cgactcgcag tagggcctcg cggcgaacgt gcagggtcaa ctcgttgcga tagaccacga ctttctctat ggcatcctcg aattcgacgc cgtcgcggtt cagcgcctcg gccaggcgat cggcgatctc gtcgaagtag aagccgtacg ggcgggggct actgcccggc agcacgatct cgcgcaccaa tcgcccgtat ccggaggtgt cgccggtgcc tttgacgccg aacatgccgc gacgcacgtc gaccacttct tcattggagg agccggtttg gcccgctgcg tctgatgggt tctggtctgg cgagctcatc gaaccaggac cctgggtctg agcttccagg gtacccatat gatggtgatg gtgatgagcc at
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSSPDQNPS DAAGQTGSSN EEVVDVRRGM FGVKGTGDTS GYGRLVREIV LPGSSPRPYG FYFDEIADRL AEALNRDGVE FEDAIEKVVV YRNELTLHVR REALLRVAQS LRDEPELRFE LCLGVNGVHY PHETGRELHA VYPLQSITHN RRLRLEVSAP DSDPHIPSLY AVYPTNDWHE RETYDFFGII FDGHPSLTRI EMPDDWQGHP QRKDYPLGGI PVEYKGAQIP PPDERRGYN
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagctcgcca gaccagaacc catcagacgc agcgggccaa accggctcct ccaatgaaga agtggtcgac gtgcgtcgcg gcatgttcgg cgtcaaaggc accggcgaca cctccggata cgggcgattg gtgcgcgaga tcgtgctgcc gggcagtagc ccccgcccgt acggcttcta cttcgacgag atcgccgatc gcctggccga ggcgctgaac cgcgacggcg tcgaattcga ggatgccata gagaaagtcg tggtctatcg caacgagttg accctgcacg ttcgccgcga ggccctactg cgagtcgcac agtccctccg cgacgagccg gaattgcgct ttgagctgtg cctgggagtc aacggggtgc actacccgca tgagaccggc cgcgaactac acgcggttta cccgctgcaa tcgatcaccc acaaccgtcg cctgcggctg gaagtgtctg cccccgacag cgatccgcat atcccgtcgc tatacgccgt ctacccgacc aacgattggc acgagcgcga gacatacgac ttcttcggca tcatcttcga cgggcatccc tcgctgacgc gaatcgagat gcctgacgac tggcaggggc acccacagcg caaggactat ccactcggcg gcatcccggt cgaatacaag ggcgcgcaga tacccccgcc cgacgagcga aggggttaca acaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa
Details for MymaA.01073.a.A1.PW30007
PURIFICATION DATe: 11/15/2010
CONCENTRATION: 26.56mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 3
VIAL VOLUME: 100µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSSPDQNPS DAAGQTGSSN EEVVDVRRGM FGVKGTGDTS GYGRLVREIV LPGSSPRPYG FYFDEIADRL AEALNRDGVE FEDAIEKVVV YRNELTLHVR REALLRVAQS LRDEPELRFE LCLGVNGVHY PHETGRELHA VYPLQSITHN RRLRLEVSAP DSDPHIPSLY AVYPTNDWHE RETYDFFGII FDGHPSLTRI EMPDDWQGHP QRKDYPLGGI PVEYKGAQIP PPDERRG
Validated NT Sequence
aaccccttcg ctcgtcgggc gggggtatct gcgcgccctt gtattcgacc gggatgccgc cgagtggata gtccttgcgc tgtgggtgcc cctgccagtc gtcaggcatc tcgattcgcg tcagcgaggg atgcccgtcg aagatgatgc cgaagaagtc gtatgtctcg cgctcgtgcc aatcgttggt cgggtagacg gcgtatagcg acgggatatg cggatcgctg tcgggggcag acacttccag ccgcaggcga cggttgtggg tgatcgattg cagcgggtaa accgcgtgta gttcgcggcc ggtctcatgc gggtagtgca ccccgttgac tcccaggcac agctcaaagc gcaattccgg ctcgtcgcgg agggactgtg cgactcgcag tagggcctcg cggcgaacgt gcagggtcaa ctcgttgcga tagaccacga ctttctctat ggcatcctcg aattcgacgc cgtcgcggtt cagcgcctcg gccaggcgat cggcgatctc gtcgaagtag aagccgtacg ggcgggggct actgcccggc agcacgatct cgcgcaccaa tcgcccgtat ccggaggtgt cgccggtgcc tttgacgccg aacatgccgc gacgcacgtc gaccacttct tcattggagg agccggtttg gcccgctgcg tctgatgggt tctggtctgg cgagctcatc gaaccaggac cctgggtctg agcttccagg gtacccatat gatggtgatg gtgatgagcc at
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSSPDQNPS DAAGQTGSSN EEVVDVRRGM FGVKGTGDTS GYGRLVREIV LPGSSPRPYG FYFDEIADRL AEALNRDGVE FEDAIEKVVV YRNELTLHVR REALLRVAQS LRDEPELRFE LCLGVNGVHY PHETGRELHA VYPLQSITHN RRLRLEVSAP DSDPHIPSLY AVYPTNDWHE RETYDFFGII FDGHPSLTRI EMPDDWQGHP QRKDYPLGGI PVEYKGAQIP PPDERRGYN
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAGCTCGCCA GACCAGAACC CATCAGACGC AGCGGGCCAA ACCGGCTCCT CCAATGAAGA AGTGGTCGAC GTGCGTCGCG GCATGTTCGG CGTCAAAGGC ACCGGCGACA CCTCCGGATA CGGGCGATTG GTGCGCGAGA TCGTGCTGCC GGGCAGTAGC CCCCGCCCGT ACGGCTTCTA CTTCGACGAG ATCGCCGATC GCCTGGCCGA GGCGCTGAAC CGCGACGGCG TCGAATTCGA GGATGCCATA GAGAAAGTCG TGGTCTATCG CAACGAGTTG ACCCTGCACG TTCGCCGCGA GGCCCTACTG CGAGTCGCAC AGTCCCTCCG CGACGAGCCG GAATTGCGCT TTGAGCTGTG CCTGGGAGTC AACGGGGTGC ACTACCCGCA TGAGACCGGC CGCGAACTAC ACGCGGTTTA CCCGCTGCAA TCGATCACCC ACAACCGTCG CCTGCGGCTG GAAGTGTCTG CCCCCGACAG CGATCCGCAT ATCCCGTCGC TATACGCCGT CTACCCGACC AACGATTGGC ACGAGCGCGA GACATACGAC TTCTTCGGCA TCATCTTCGA CGGGCATCCC TCGCTGACGC GAATCGAGAT GCCTGACGAC TGGCAGGGGC ACCCACAGCG CAAGGACTAT CCACTCGGCG GCATCCCGGT CGAATACAAG GGCGCGCAGA TACCCCCGCC CGACGAGCGA AGGGGTTACA ACAAACAGCA CGAACAAGTT CTGCAGCCAA GCTTCTCGAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAA