SSGCID: Target Page

MyabA.01073.a

NADH-quinone oxidoreductase subunit C

CENTER ID:	MyabA.01073.a
ORGANISM:	Mycobacterium abscessus ATCC 19977 / DSM 44196
ASSOCIATED DISEASE:	Skin abscess
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyabA.01073.a.A1.GE30359	Full length( MyabA.01073.a )		1	228

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|36809.5.peg.2175
RefSeq:	YP_001702871.1
UniProt:	B1MPG6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTGGEQKPNE IIGVRRGLFG VRGSGDTSGY GGLVQPISLP VSSNRPYGGY FDQVVDRLES VLAEQENVTY ADAVEGVVVY RDQLTIHVKA EHLVQVAQSL RDDPQLRFEL SLGVSGVHYP DDSARELHAV YPLMSITWNR RIMLEVAVPE SDPHIPSLNA VYPTTDWHER ETYDFFGIIF DDHPALTRIQ MPDDWDGHPQ RKDYPLGGVP VEYHGATIAP PDQRRSYN

NT Sequence

GTGACCGGCG GCGAGCAGAA ACCGAATGAA ATAATCGGGG TGCGGCGCGG GCTCTTTGGG GTGCGGGGGA GCGGCGACAC ATCCGGCTAC GGCGGTCTAG TCCAACCGAT TTCCTTGCCG GTTAGCTCGA ACCGTCCGTA CGGCGGGTAC TTCGACCAGG TGGTCGACAG GCTGGAATCG GTTCTCGCAG AGCAGGAGAA CGTTACCTAC GCGGATGCTG TCGAAGGTGT GGTCGTGTAT CGCGATCAAC TGACCATTCA CGTGAAAGCC GAGCATCTGG TTCAGGTCGC CCAATCGCTG CGCGATGATC CGCAACTGCG CTTCGAGCTC AGTCTCGGTG TGAGCGGGGT GCACTATCCC GATGACTCTG CGCGGGAATT GCATGCCGTT TACCCGCTCA TGTCCATCAC CTGGAATCGC CGGATCATGC TGGAAGTAGC TGTACCGGAA AGCGATCCGC ACATCCCTTC GCTCAATGCC GTCTACCCGA CCACGGATTG GCATGAACGC GAAACCTACG ACTTCTTCGG AATCATCTTT GATGATCATC CCGCACTGAC GCGCATACAG ATGCCCGACG ACTGGGACGG GCACCCGCAG CGCAAGGACT ACCCGCTGGG TGGTGTGCCG GTCGAGTATC ACGGAGCAAC CATTGCACCG CCCGATCAGC GGAGGTCTTA CAAC

Details for MyabA.01073.a.A1.GE30359

HARVESTED ON:	9/8/2010
SEQUENCED ON:	9/17/2010
EXPECTED MW:	27kDa
OBSERVED MW:	27kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMVTGGEQKP NEIIGVRRGL FGVRGSGDTS GYGGLVQPIS LPVSSNRPYG GYFDQVVDRL ESVLAEQENV TYADAVEGVV VYRDQLTIHV KAEHLVQVAQ SLRDDPQLRF ELSLGVSGVH YPDDSARELH AVYPLMSITW NRRIMLEVAV PESDPHIPSL NAVYPTTDWH ERETYDFFGI IFDDHPALTR IQMPDDWDGH PQRKDYPLGG VPVEYHGATI APPDQRXSYN

Validated NT Sequence

ttntttactt taagaaggag atataccatg gctcatcacc atcaccatca tatgggtacc ctggaagctc agacccaggg tcctggttcg atggtgaccg gcggcgagca gaaaccgaat gaaataatcg gggtgcggcg cgggctcttt ggggtgcggg ggagcggcga cacatccggc tacggcggtc tagtccaacc gatttccttg ccggttagct cgaaccgtcc gtacggcggg tacttcgacc aggtggtcga caggctggaa tcggttctcg cagagcagga gaacgttacc tacgcggatg ctgtcgaagg tgtggtcgtg tatcgcgatc aactgaccat tcacgtgaaa gccgagcatc tggttcaggt cgcccaatcg ctgcgcgatg atccgcaact gcgcttcgag ctcagtctcg gtgtgagcgg ggtgcactat cccgatgact ctgcgcggga attgcatgcc gtttacccgc tcatgtccat cacctggaat cgccggatca tgctggaagt agctgtaccg gaaagcgatc cgcacatccc ttcgctcaat gccgtctacc cgaccacgga ttggcatgaa cgcgaaacct acgacttctt cggaatcatc tttgatgatc atcccgcact gacgcgcata cagatgcccg acgactggga cgggcacccg cagcgcaagg actacccgct gggtggtgtg ccggtcgagt atcacggagc aaccattgca ccgcccgatc agcggangtc ttacaactaa taaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgnnncc gctnannnnn nnta

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgaccggc ggcgagcaga aaccgaatga aataatcggg gtgcggcgcg ggctctttgg ggtgcggggg agcggcgaca catccggcta cggcggtcta gtccaaccga tttccttgcc ggttagctcg aaccgtccgt acggcgggta cttcgaccag gtggtcgaca ggctggaatc ggttctcgca gagcaggaga acgttaccta cgcggatgct gtcgaaggtg tggtcgtgta tcgcgatcaa ctgaccattc acgtgaaagc cgagcatctg gttcaggtcg cccaatcgct gcgcgatgat ccgcaactgc gcttcgagct cagtctcggt gtgagcgggg tgcactatcc cgatgactct gcgcgggaat tgcatgccgt ttacccgctc atgtccatca cctggaatcg ccggatcatg ctggaagtag ctgtaccgga aagcgatccg cacatccctt cgctcaatgc cgtctacccg accacggatt ggcatgaacg cgaaacctac gacttcttcg gaatcatctt tgatgatcat cccgcactga cgcgcataca gatgcccgac gactgggacg ggcacccgca gcgcaaggac tacccgctgg gtggtgtgcc ggtcgagtat cacggagcaa ccattgcacc gcccgatcag cggaggtctt acaactaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa