MypaA.01073.a

NADH-quinone oxidoreductase subunit C

CENTER ID: MypaA.01073.a
ORGANISM: Mycobacterium paratuberculosis ATCC BAA-968 / K-10
ASSOCIATED DISEASE: Johne's disease
CURRENT STATUS: crystallized
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MypaA.01073.a.A1.GE29535 Full length( MypaA.01073.a ) 1 235
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MypaA.01073.a.A1.PS00756 Full length( MypaA.01073.a ) 1 235

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|262316.17.peg.3399
RefSeq: NP_962137.1
UniProt: Q73V13

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSSPDQDPRE AIAQGDDEVI DVRRGMFGAA GTGDTSGYGR LVRTVTLPGS SPRPYGSYFD DVVDTLTESL QSNGIEFHQA IEKVVVYRDE LTLHVDRAAL PHVAQHLRDD PRLRFEMCLG VSGVHYPHET GRELHAVYPL QSITHNRRVR LEVAVPDEDP HIPSLYRIYP TTDWHERETY DFFGIVFDGH PSLTRIEMPD DWHGHPQRKD YPLGGIPVEY KGAQIPPPDE RRAYN
NT Sequence
ATGAGCTCGC CCGACCAGGA CCCCCGCGAG GCGATAGCCC AGGGCGACGA CGAGGTGATC GACGTTCGTC GCGGCATGTT CGGGGCCGCC GGCACCGGCG ACACGTCCGG CTACGGGCGG CTGGTGCGCA CGGTCACCCT GCCCGGCAGC AGCCCGCGAC CCTACGGCAG CTACTTCGAC GACGTCGTCG ACACGTTGAC CGAGTCGTTG CAGAGCAACG GCATCGAATT CCACCAGGCG ATCGAGAAGG TCGTGGTGTA CCGCGACGAG CTCACCCTGC ACGTCGACCG GGCCGCACTG CCGCACGTGG CCCAGCACCT TCGCGACGAC CCGCGGCTGC GTTTCGAGAT GTGCCTGGGC GTTAGCGGCG TGCACTACCC GCACGAGACG GGTCGCGAAC TGCACGCCGT CTATCCGCTG CAATCCATCA CGCACAACCG CCGCGTCCGC CTGGAAGTGG CTGTGCCCGA CGAGGATCCG CACATCCCGT CGCTGTACCG GATCTATCCG ACCACCGACT GGCACGAGCG CGAGACCTAC GACTTCTTCG GCATCGTCTT CGACGGGCAC CCGTCGCTGA CCCGCATCGA GATGCCGGAC GACTGGCACG GGCATCCGCA GCGCAAGGAC TACCCGCTCG GCGGGATCCC GGTCGAATAC AAAGGCGCAC AGATACCCCC GCCCGACGAG CGGAGAGCGT ACAAC
Details for MypaA.01073.a.A1.GE29535
HARVESTED ON: 6/15/2010
SEQUENCED ON: 6/27/2010
EXPECTED MW: 28kDa
OBSERVED MW: 29kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSSPDQDPR EAIAQGDDEV IDVRRGMFGA AGTGDTSGYG RLVRTVTLPG SSPRPYGSYF DDVVDTLTES LQSNGIEFHQ AIEKVVVYRD ELTLHVDRAA LPHVAQHLRD DPRLRFEMCL GVSGVHYPHE TGRELHAVYP LQSITHNRRV RLEVAVPDED PHIPSLYRIY PTTDWHERET YDFFGIVFDG HPSLTRIEMP DDWHGHPQRK DYPLGGIPVE YKGAQIPPPD ERRA
Validated NT Sequence
acgctctccg ctcgtcgggc gggggtatct gtgcgccttt gtattcgacc gggatcccgc cgagcgggta gtccttgcgc tgcggatgcc cgtgccagtc gtccggcatc tcgatgcggg tcagcgacgg gtgcccgtcg aagacgatgc cgaagaagtc gtaggtctcg cgctcgtgcc agtcggtggt cggatagatc cggtacagcg acgggatgtg cggatcctcg tcgggcacag ccacttccag gcggacgcgg cggttgtgcg tgatggattg cagcggatag acggcgtgca gttcgcgacc cgtctcgtgc gggtagtgca cgccgctaac gcccaggcac atctcgaaac gcagccgcgg gtcgtcgcga aggtgctggg ccacgtgcgg cagtgcggcc cggtcgacgt gcagggtgag ctcgtcgcgg tacaccacga ccttctcgat cgcctggtgg aattcgatgc cgttgctctg caacgactcg gtcaacgtgt cgacgacgtc gtcgaagtag ctgccgtagg gtcgcgggct gctgccgggc agggtgaccg tgcgcaccag ccgcccgtag ccggacgtgt cgccggtgcc ggcggccccg aacatgccgc gacgaacgtc gatcacctcg tcgtcgccct gggctatcgc ctcgcggggg tcctggtcgg gcgagctcat cgaaccagga ccctgggtct gagcttccag ggtacccata tgatggtgat ggtgatgagc cat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSSPDQDPR EAIAQGDDEV IDVRRGMFGA AGTGDTSGYG RLVRTVTLPG SSPRPYGSYF DDVVDTLTES LQSNGIEFHQ AIEKVVVYRD ELTLHVDRAA LPHVAQHLRD DPRLRFEMCL GVSGVHYPHE TGRELHAVYP LQSITHNRRV RLEVAVPDED PHIPSLYRIY PTTDWHERET YDFFGIVFDG HPSLTRIEMP DDWHGHPQRK DYPLGGIPVE YKGAQIPPPD ERRAYN
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagctcgccc gaccaggacc cccgcgaggc gatagcccag ggcgacgacg aggtgatcga cgttcgtcgc ggcatgttcg gggccgccgg caccggcgac acgtccggct acgggcggct ggtgcgcacg gtcaccctgc ccggcagcag cccgcgaccc tacggcagct acttcgacga cgtcgtcgac acgttgaccg agtcgttgca gagcaacggc atcgaattcc accaggcgat cgagaaggtc gtggtgtacc gcgacgagct caccctgcac gtcgaccggg ccgcactgcc gcacgtggcc cagcaccttc gcgacgaccc gcggctgcgt ttcgagatgt gcctgggcgt tagcggcgtg cactacccgc acgagacggg tcgcgaactg cacgccgtct atccgctgca atccatcacg cacaaccgcc gcgtccgcct ggaagtggct gtgcccgacg aggatccgca catcccgtcg ctgtaccgga tctatccgac caccgactgg cacgagcgcg agacctacga cttcttcggc atcgtcttcg acgggcaccc gtcgctgacc cgcatcgaga tgccggacga ctggcacggg catccgcagc gcaaggacta cccgctcggc gggatcccgg tcgaatacaa aggcgcacag atacccccgc ccgacgagcg gagagcgtac aacaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa
Details for MypaA.01073.a.A1.PS00756
PURIFICATION DATe: 8/31/2010
CONCENTRATION: 23.61mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 8
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSSPDQDPR EAIAQGDDEV IDVRRGMFGA AGTGDTSGYG RLVRTVTLPG SSPRPYGSYF DDVVDTLTES LQSNGIEFHQ AIEKVVVYRD ELTLHVDRAA LPHVAQHLRD DPRLRFEMCL GVSGVHYPHE TGRELHAVYP LQSITHNRRV RLEVAVPDED PHIPSLYRIY PTTDWHERET YDFFGIVFDG HPSLTRIEMP DDWHGHPQRK DYPLGGIPVE YKGAQIPPPD ERRA
Validated NT Sequence
acgctctccg ctcgtcgggc gggggtatct gtgcgccttt gtattcgacc gggatcccgc cgagcgggta gtccttgcgc tgcggatgcc cgtgccagtc gtccggcatc tcgatgcggg tcagcgacgg gtgcccgtcg aagacgatgc cgaagaagtc gtaggtctcg cgctcgtgcc agtcggtggt cggatagatc cggtacagcg acgggatgtg cggatcctcg tcgggcacag ccacttccag gcggacgcgg cggttgtgcg tgatggattg cagcggatag acggcgtgca gttcgcgacc cgtctcgtgc gggtagtgca cgccgctaac gcccaggcac atctcgaaac gcagccgcgg gtcgtcgcga aggtgctggg ccacgtgcgg cagtgcggcc cggtcgacgt gcagggtgag ctcgtcgcgg tacaccacga ccttctcgat cgcctggtgg aattcgatgc cgttgctctg caacgactcg gtcaacgtgt cgacgacgtc gtcgaagtag ctgccgtagg gtcgcgggct gctgccgggc agggtgaccg tgcgcaccag ccgcccgtag ccggacgtgt cgccggtgcc ggcggccccg aacatgccgc gacgaacgtc gatcacctcg tcgtcgccct gggctatcgc ctcgcggggg tcctggtcgg gcgagctcat cgaaccagga ccctgggtct gagcttccag ggtacccata tgatggtgat ggtgatgagc cat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSSPDQDPR EAIAQGDDEV IDVRRGMFGA AGTGDTSGYG RLVRTVTLPG SSPRPYGSYF DDVVDTLTES LQSNGIEFHQ AIEKVVVYRD ELTLHVDRAA LPHVAQHLRD DPRLRFEMCL GVSGVHYPHE TGRELHAVYP LQSITHNRRV RLEVAVPDED PHIPSLYRIY PTTDWHERET YDFFGIVFDG HPSLTRIEMP DDWHGHPQRK DYPLGGIPVE YKGAQIPPPD ERRAYN
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAGCTCGCCC GACCAGGACC CCCGCGAGGC GATAGCCCAG GGCGACGACG AGGTGATCGA CGTTCGTCGC GGCATGTTCG GGGCCGCCGG CACCGGCGAC ACGTCCGGCT ACGGGCGGCT GGTGCGCACG GTCACCCTGC CCGGCAGCAG CCCGCGACCC TACGGCAGCT ACTTCGACGA CGTCGTCGAC ACGTTGACCG AGTCGTTGCA GAGCAACGGC ATCGAATTCC ACCAGGCGAT CGAGAAGGTC GTGGTGTACC GCGACGAGCT CACCCTGCAC GTCGACCGGG CCGCACTGCC GCACGTGGCC CAGCACCTTC GCGACGACCC GCGGCTGCGT TTCGAGATGT GCCTGGGCGT TAGCGGCGTG CACTACCCGC ACGAGACGGG TCGCGAACTG CACGCCGTCT ATCCGCTGCA ATCCATCACG CACAACCGCC GCGTCCGCCT GGAAGTGGCT GTGCCCGACG AGGATCCGCA CATCCCGTCG CTGTACCGGA TCTATCCGAC CACCGACTGG CACGAGCGCG AGACCTACGA CTTCTTCGGC ATCGTCTTCG ACGGGCACCC GTCGCTGACC CGCATCGAGA TGCCGGACGA CTGGCACGGG CATCCGCAGC GCAAGGACTA CCCGCTCGGC GGGATCCCGG TCGAATACAA AGGCGCACAG ATACCCCCGC CCGACGAGCG GAGAGCGTAC AACAAACAGC ACGAACAAGT TCTGCAGCCA AGCTTCTCGA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAA