MytuD.01048.a

PROBABLE ACYL-CoA DEHYDROGENASE FADE9

CENTER ID: MytuD.01048.a
ORGANISM: Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE: Tuberculosis
CURRENT STATUS: crystallized
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MytuD.01048.a.A1.GE25927 Full length( MytuD.01048.a ) 1 390
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MytuD.01048.a.A1.PW26032 Full length( MytuD.01048.a ) 1 390

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|83332.12.peg.841
RefSeq: NP_215266.1
TubercuList: Rv0752c
MTB Network Portal: Rv0752c
UniProt: I6Y4R2

Orthologues for Rv0752c

Species % Identity % Coverage Clones Proteins Structures
marinum 90 100 1 0 0
ulcerans 90 100 1 1 0
avium 88 100 0 0 0
paratuberculosis 88 100 1 1 0
smegmatis 78 99 0 1 0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MFVLNDDERV IVETAAAFAG KRLAPHALEW DAAKHFPVDV LREAAELGMA AIYCRDDVGG SGLRRLDGVR IFEQLAIADP VTAAFLSIHN MCAWMIDSFG TDEQRKDWIP RLATMGVIAS YCLTEPGAGS DAGALSTRAV RHGSGKGGDY VLDGVKQFIS GAAASDVYVV MARTGAEGPR GVSAFIVEKG TPGLSFGAPE AKMGWHAQPT AQVVLDGVRV PAEAMLGGAD GEGAGFGIAM SGLNGGRLNI AACSLGGAQA AFDKAGAYVR DRQAFGGSLL DEPTVRFTLA DMATGLQTSR MLLWRAASAL DDDDADKVEL CAMAKRYVTD TCFEVADQAL QLHGGYGYLR EYGLEKIVRD LRVHRILEGT NEIMRLVIGR AEAARFRATV
NT Sequence
ATGTTTGTCC TTAACGACGA CGAACGGGTG ATCGTCGAGA CGGCGGCCGC CTTCGCCGGC AAACGCCTGG CTCCGCACGC CCTGGAATGG GATGCCGCCA AACACTTTCC GGTGGACGTG TTGCGGGAAG CGGCCGAACT CGGCATGGCC GCGATCTATT GCCGCGACGA CGTCGGCGGC AGTGGGCTGC GCCGGCTCGA CGGCGTGCGC ATCTTCGAGC AGTTGGCGAT CGCCGACCCG GTGACCGCCG CGTTTTTGTC CATCCACAAC ATGTGCGCGT GGATGATTGA CAGCTTCGGC ACCGACGAGC AACGCAAGGA CTGGATTCCG CGACTGGCCA CCATGGGCGT CATCGCCAGC TACTGCCTGA CCGAACCGGG CGCCGGATCC GATGCCGGCG CACTGAGCAC CCGCGCCGTC AGGCACGGCT CAGGAAAAGG CGGCGACTAC GTGCTCGATG GCGTCAAACA ATTCATTTCC GGGGCCGCAG CATCGGACGT GTATGTGGTG ATGGCGCGCA CGGGCGCCGA GGGTCCGCGC GGCGTGTCGG CGTTCATCGT CGAAAAGGGC ACACCGGGGC TGAGTTTCGG CGCGCCCGAA GCGAAGATGG GTTGGCATGC CCAACCCACC GCGCAGGTGG TGCTGGACGG GGTGCGGGTA CCCGCCGAGG CCATGCTGGG CGGCGCCGAC GGCGAAGGCG CCGGCTTCGG TATCGCGATG AGCGGCCTCA ACGGCGGCCG GCTCAATATC GCAGCATGCT CGCTGGGTGG CGCGCAGGCC GCCTTTGACA AAGCGGGCGC CTACGTTCGC GACCGACAGG CCTTCGGCGG ATCGCTGCTC GACGAGCCCA CCGTGCGGTT TACCCTGGCC GACATGGCTA CCGGGCTGCA GACGTCGCGA ATGTTGTTGT GGCGGGCCGC AAGTGCGCTC GATGACGACG ACGCCGACAA GGTCGAGCTG TGTGCGATGG CCAAGCGCTA CGTCACCGAC ACCTGCTTTG AGGTCGCCGA CCAGGCCCTG CAGCTGCACG GCGGCTATGG CTATCTGCGC GAGTATGGTC TGGAGAAGAT CGTCCGCGAC CTGCGGGTGC ATCGCATCCT GGAAGGGACC AACGAGATCA TGCGGCTGGT CATCGGTCGG GCCGAGGCCG CGCGGTTCCG CGCCACCGTT
Details for MytuD.01048.a.A1.GE25927
HARVESTED ON: 1/9/2009
SEQUENCED ON: 8/18/2010
EXPECTED MW: 43kDa
OBSERVED MW: 44kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMFVLNDDER VIVETAAAFA GKRLAPHALE WDAAKHFPVD VLREAAELGM AAIYCRDDVG GSGLRRLDGV RIFEQLAIAD PVTAAFLSIH NMCAWMIDSF GTDEQRKDWI PRLATMGVIA SYCLTEPGAG SDAGALSTRA VRHGSGKGGD YVLDGVKQFI SGAAASDVYV VMARTGAEGP RGVSAFIVEK GTPGLSFGAP EAKMGWHAQP TAQVVLDGVR VPAEAMLGGA DGEGAGFGIA MSGLNGGRLN IAACSLGGAQ AAFDKAGAYV RDRQAFGGSL LDEPTVRFTL ADMATGLQTS RMLLWRAASA LDDDDADKVE LCAMAKRYVT DTCFEVADQA LQLHGGYGYL REYGLEKIVR DLRVHRILEG TNEIMRLVIG RAEAARFRAT V
Validated NT Sequence
ttattaaacg gtggcgcgga accgcgcggc ctcggcccga ccgatgacca gccgcatgat ctcgttggtc ccttccagga tgcgatgcac ccgcaggtcg cggacgatct tctccagacc atactcgcgc agatagccat agccgccgtg cagctgcagg gcctggtcgg cgacctcaaa gcaggtgtcg gtgacgtagc gcttggccat cgcacacagc tcgaccttgt cggcgtcgtc gtcatcgagc gcacttgcgg cccgccacaa caacattcgc gacgtctgca gcccggtagc catgtcggcc agggtaaacc gcacggtggg ctcgtcgagc agcgatccgc cgaaggcctg tcggtcgcga acgtaggcgc ccgctttgtc aaaggcggcc tgcgcgccac ccagcgagca tgctgcgata ttgagccggc cgccgttgag gccgctcatc gcgataccga agccggcgcc ttcgccgtcg gcgccgccca gcatggcctc ggcgggtacc cgcaccccgt ccagcaccac ctgcgcggtg ggttgggcat gccaacccat cttcgcttcg ggcgcgccga aactcagccc cggtgtgccc ttttcgacga tgaacgccga cacgccgcgc ggaccctcgg cgcccgtgcg cgccatcacc acatacacgt ccgatgctgc ggccccggaa atgaattgtt tgacgccatc gagcacgtag tcgccgcctt ttcctgagcc gtgcctgacg gcgcgggtgc tcagtgcgcc ggcatcggat ccggcgcccg gttcggtcag gcagtagctg gcgatgacgc ccatggtggc cagtcgcgga atccagtcct tgcgttgctc gtcggtgccg aagctgtcaa tcatccacgc gcacatgttg tggatggaca aaaacgcggc ggtcaccggg tcggcgatcg ccaactgctc gaagatgcgc acgccgtcga gccggcgcag cccactgccg ccgacgtcgt cgcggcaata gatcgcggcc atgccgagtt cggccgcttc ccgcaacacg tccaccggaa agtgtttggc ggcatcccat tccagggcgt gcggagccag gcgtttgccg gcgaaggcgg ccgccgtctc gacgatcacc cgttcgtcgt cgttaaggac aaacatcgaa ccaggaccct gggtctgagc ttccagggta cccatatgat ggtgatggtg atgagccat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMFVLNDDER VIVETAAAFA GKRLAPHALE WDAAKHFPVD VLREAAELGM AAIYCRDDVG GSGLRRLDGV RIFEQLAIAD PVTAAFLSIH NMCAWMIDSF GTDEQRKDWI PRLATMGVIA SYCLTEPGAG SDAGALSTRA VRHGSGKGGD YVLDGVKQFI SGAAASDVYV VMARTGAEGP RGVSAFIVEK GTPGLSFGAP EAKMGWHAQP TAQVVLDGVR VPAEAMLGGA DGEGAGFGIA MSGLNGGRLN IAACSLGGAQ AAFDKAGAYV RDRQAFGGSL LDEPTVRFTL ADMATGLQTS RMLLWRAASA LDDDDADKVE LCAMAKRYVT DTCFEVADQA LQLHGGYGYL REYGLEKIVR DLRVHRILEG TNEIMRLVIG RAEAARFRAT V
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtttgtcctt aacgacgacg aacgggtgat cgtcgagacg gcggccgcct tcgccggcaa acgcctggct ccgcacgccc tggaatggga tgccgccaaa cactttccgg tggacgtgtt gcgggaagcg gccgaactcg gcatggccgc gatctattgc cgcgacgacg tcggcggcag tgggctgcgc cggctcgacg gcgtgcgcat cttcgagcag ttggcgatcg ccgacccggt gaccgccgcg tttttgtcca tccacaacat gtgcgcgtgg atgattgaca gcttcggcac cgacgagcaa cgcaaggact ggattccgcg actggccacc atgggcgtca tcgccagcta ctgcctgacc gaaccgggcg ccggatccga tgccggcgca ctgagcaccc gcgccgtcag gcacggctca ggaaaaggcg gcgactacgt gctcgatggc gtcaaacaat tcatttccgg ggccgcagca tcggacgtgt atgtggtgat ggcgcgcacg ggcgccgagg gtccgcgcgg cgtgtcggcg ttcatcgtcg aaaagggcac accggggctg agtttcggcg cgcccgaagc gaagatgggt tggcatgccc aacccaccgc gcaggtggtg ctggacgggg tgcgggtacc cgccgaggcc atgctgggcg gcgccgacgg cgaaggcgcc ggcttcggta tcgcgatgag cggcctcaac ggcggccggc tcaatatcgc agcatgctcg ctgggtggcg cgcaggccgc ctttgacaaa gcgggcgcct acgttcgcga ccgacaggcc ttcggcggat cgctgctcga cgagcccacc gtgcggttta ccctggccga catggctacc gggctgcaga cgtcgcgaat gttgttgtgg cgggccgcaa gtgcgctcga tgacgacgac gccgacaagg tcgagctgtg tgcgatggcc aagcgctacg tcaccgacac ctgctttgag gtcgccgacc aggccctgca gctgcacggc ggctatggct atctgcgcga gtatggtctg gagaagatcg tccgcgacct gcgggtgcat cgcatcctgg aagggaccaa cgagatcatg cggctggtca tcggtcgggc cgaggccgcg cggttccgcg ccaccgttaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa
Details for MytuD.01048.a.A1.PW26032
PURIFICATION DATe: 2/3/2009
CONCENTRATION: 24.5mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 12
VIAL VOLUME: 100µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMFVLNDDER VIVETAAAFA GKRLAPHALE WDAAKHFPVD VLREAAELGM AAIYCRDDVG GSGLRRLDGV RIFEQLAIAD PVTAAFLSIH NMCAWMIDSF GTDEQRKDWI PRLATMGVIA SYCLTEPGAG SDAGALSTRA VRHGSGKGGD YVLDGVKQFI SGAAASDVYV VMARTGAEGP RGVSAFIVEK GTPGLSFGAP EAKMGWHAQP TAQVVLDGVR VPAEAMLGGA DGEGAGFGIA MSGLNGGRLN IAACSLGGAQ AAFDKAGAYV RDRQAFGGSL LDEPTVRFTL ADMATGLQTS RMLLWRAASA LDDDDADKVE LCAMAKRYVT DTCFEVADQA LQLHGGYGYL REYGLEKIVR DLRVHRILEG TNEIMRLVIG RAEAARFRAT V
Validated NT Sequence
ttattaaacg gtggcgcgga accgcgcggc ctcggcccga ccgatgacca gccgcatgat ctcgttggtc ccttccagga tgcgatgcac ccgcaggtcg cggacgatct tctccagacc atactcgcgc agatagccat agccgccgtg cagctgcagg gcctggtcgg cgacctcaaa gcaggtgtcg gtgacgtagc gcttggccat cgcacacagc tcgaccttgt cggcgtcgtc gtcatcgagc gcacttgcgg cccgccacaa caacattcgc gacgtctgca gcccggtagc catgtcggcc agggtaaacc gcacggtggg ctcgtcgagc agcgatccgc cgaaggcctg tcggtcgcga acgtaggcgc ccgctttgtc aaaggcggcc tgcgcgccac ccagcgagca tgctgcgata ttgagccggc cgccgttgag gccgctcatc gcgataccga agccggcgcc ttcgccgtcg gcgccgccca gcatggcctc ggcgggtacc cgcaccccgt ccagcaccac ctgcgcggtg ggttgggcat gccaacccat cttcgcttcg ggcgcgccga aactcagccc cggtgtgccc ttttcgacga tgaacgccga cacgccgcgc ggaccctcgg cgcccgtgcg cgccatcacc acatacacgt ccgatgctgc ggccccggaa atgaattgtt tgacgccatc gagcacgtag tcgccgcctt ttcctgagcc gtgcctgacg gcgcgggtgc tcagtgcgcc ggcatcggat ccggcgcccg gttcggtcag gcagtagctg gcgatgacgc ccatggtggc cagtcgcgga atccagtcct tgcgttgctc gtcggtgccg aagctgtcaa tcatccacgc gcacatgttg tggatggaca aaaacgcggc ggtcaccggg tcggcgatcg ccaactgctc gaagatgcgc acgccgtcga gccggcgcag cccactgccg ccgacgtcgt cgcggcaata gatcgcggcc atgccgagtt cggccgcttc ccgcaacacg tccaccggaa agtgtttggc ggcatcccat tccagggcgt gcggagccag gcgtttgccg gcgaaggcgg ccgccgtctc gacgatcacc cgttcgtcgt cgttaaggac aaacatcgaa ccaggaccct gggtctgagc ttccagggta cccatatgat ggtgatggtg atgagccat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMFVLNDDER VIVETAAAFA GKRLAPHALE WDAAKHFPVD VLREAAELGM AAIYCRDDVG GSGLRRLDGV RIFEQLAIAD PVTAAFLSIH NMCAWMIDSF GTDEQRKDWI PRLATMGVIA SYCLTEPGAG SDAGALSTRA VRHGSGKGGD YVLDGVKQFI SGAAASDVYV VMARTGAEGP RGVSAFIVEK GTPGLSFGAP EAKMGWHAQP TAQVVLDGVR VPAEAMLGGA DGEGAGFGIA MSGLNGGRLN IAACSLGGAQ AAFDKAGAYV RDRQAFGGSL LDEPTVRFTL ADMATGLQTS RMLLWRAASA LDDDDADKVE LCAMAKRYVT DTCFEVADQA LQLHGGYGYL REYGLEKIVR DLRVHRILEG TNEIMRLVIG RAEAARFRAT V
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTTTGTCCTT AACGACGACG AACGGGTGAT CGTCGAGACG GCGGCCGCCT TCGCCGGCAA ACGCCTGGCT CCGCACGCCC TGGAATGGGA TGCCGCCAAA CACTTTCCGG TGGACGTGTT GCGGGAAGCG GCCGAACTCG GCATGGCCGC GATCTATTGC CGCGACGACG TCGGCGGCAG TGGGCTGCGC CGGCTCGACG GCGTGCGCAT CTTCGAGCAG TTGGCGATCG CCGACCCGGT GACCGCCGCG TTTTTGTCCA TCCACAACAT GTGCGCGTGG ATGATTGACA GCTTCGGCAC CGACGAGCAA CGCAAGGACT GGATTCCGCG ACTGGCCACC ATGGGCGTCA TCGCCAGCTA CTGCCTGACC GAACCGGGCG CCGGATCCGA TGCCGGCGCA CTGAGCACCC GCGCCGTCAG GCACGGCTCA GGAAAAGGCG GCGACTACGT GCTCGATGGC GTCAAACAAT TCATTTCCGG GGCCGCAGCA TCGGACGTGT ATGTGGTGAT GGCGCGCACG GGCGCCGAGG GTCCGCGCGG CGTGTCGGCG TTCATCGTCG AAAAGGGCAC ACCGGGGCTG AGTTTCGGCG CGCCCGAAGC GAAGATGGGT TGGCATGCCC AACCCACCGC GCAGGTGGTG CTGGACGGGG TGCGGGTACC CGCCGAGGCC ATGCTGGGCG GCGCCGACGG CGAAGGCGCC GGCTTCGGTA TCGCGATGAG CGGCCTCAAC GGCGGCCGGC TCAATATCGC AGCATGCTCG CTGGGTGGCG CGCAGGCCGC CTTTGACAAA GCGGGCGCCT ACGTTCGCGA CCGACAGGCC TTCGGCGGAT CGCTGCTCGA CGAGCCCACC GTGCGGTTTA CCCTGGCCGA CATGGCTACC GGGCTGCAGA CGTCGCGAAT GTTGTTGTGG CGGGCCGCAA GTGCGCTCGA TGACGACGAC GCCGACAAGG TCGAGCTGTG TGCGATGGCC AAGCGCTACG TCACCGACAC CTGCTTTGAG GTCGCCGACC AGGCCCTGCA GCTGCACGGC GGCTATGGCT ATCTGCGCGA GTATGGTCTG GAGAAGATCG TCCGCGACCT GCGGGTGCAT CGCATCCTGG AAGGGACCAA CGAGATCATG CGGCTGGTCA TCGGTCGGGC CGAGGCCGCG CGGTTCCGCG CCACCGTTAA ACAGCACGAA CAAGTTCTGC AGCCAAGCTT CTCGAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA