SSGCID: Target Page

MyulA.01048.a

Acyl-CoA dehydrogenase, FadE9

CENTER ID:	MyulA.01048.a
ORGANISM:	Mycobacterium ulcerans Agy99
ASSOCIATED DISEASE:	Buruli, Bairnsdale ulcer
CURRENT STATUS:	diffraction
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyulA.01048.a.A1.GE29760	Full length( MyulA.01048.a )		1	390

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyulA.01048.a.A1.PS01033	Full length( MyulA.01048.a )		1	390

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|362242.7.peg.1045
RefSeq:	YP_904945.1
UniProt:	A0PMA5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MFTLNDDERV ITQTAAAFAD KRLAPYALEW DATKHFPVDV LREAAELGMA AIYCREDVGG SGLRRLDGVR IFEQLAIADP VTAAFLSIHN MCTWMIDSFG TDEQRKDWIP RLATMDAIAS YCLTEPGAGS DASALSARAV RQGSAPGSDY VLDGVKQFIS GAGASDVYVV MARTGAEGPR GISAFIVEKG TPGLSFGALE EKMGWHAQPT AQVVLEGVRV PADAMLGGVD GTGAGFGIAM NGLNGGRLNI AACSLGGAQS AFAKTGAYVR ERQAFGASLL DEPTVRFTLA DMATGLEASR MLLWRAANAL DEDDADKVEL CAMAKRFVTD TCFDVADKAL QLHGGYGYLR EYGLEKIVRD LRVHRILEGT NEIMRVVVGR AEAARFRASA

NT Sequence

ATGTTTACCC TCAACGACGA CGAGCGGGTG ATCACCCAGA CCGCCGCCGC CTTCGCCGAT AAGCGGCTGG CCCCCTACGC GCTGGAATGG GATGCCACCA AGCACTTCCC GGTCGACGTG CTGCGCGAAG CCGCCGAACT GGGAATGGCG GCGATCTACT GCCGAGAAGA CGTGGGCGGT AGCGGGCTGC GCCGGCTCGA CGGCGTGCGC ATCTTCGAAC AGCTGGCCAT CGCCGACCCG GTCACCGCCG CGTTTCTGTC CATCCACAAC ATGTGCACCT GGATGATCGA CAGCTTCGGC ACCGACGAGC AACGCAAGGA CTGGATTCCG CGGCTGGCCA CGATGGACGC CATCGCCAGC TACTGCCTGA CCGAGCCGGG TGCCGGCTCC GACGCCAGCG CGTTGAGCGC CCGGGCGGTG CGGCAGGGTT CGGCCCCGGG GTCGGACTAT GTGCTCGACG GCGTCAAGCA GTTCATCTCC GGTGCGGGCG CCTCGGACGT CTACGTGGTG ATGGCGCGCA CCGGAGCCGA GGGGCCGCGC GGCATCTCGG CGTTCATTGT CGAAAAGGGC ACGCCGGGGC TGAGTTTCGG GGCGCTCGAA GAGAAGATGG GCTGGCACGC ACAGCCCACC GCACAGGTGG TGCTCGAAGG AGTTCGGGTG CCTGCCGACG CGATGCTGGG CGGCGTTGAC GGAACGGGCG CCGGATTCGG CATCGCGATG AACGGCCTCA ACGGTGGCCG CCTCAACATC GCGGCGTGTT CGCTCGGTGG CGCCCAGTCC GCCTTCGCCA AGACGGGTGC CTACGTGCGC GAGCGCCAGG CGTTCGGCGC CAGCCTGCTC GACGAGCCCA CCGTCCGGTT CACCCTGGCC GATATGGCCA CCGGTCTGGA GGCGTCGCGA ATGCTGTTGT GGCGGGCGGC AAACGCACTC GACGAGGACG ACGCGGACAA GGTGGAGCTG TGCGCGATGG CCAAGCGTTT TGTCACCGAC ACCTGCTTCG ACGTCGCCGA CAAGGCTCTG CAACTGCACG GCGGCTACGG CTACTTGCGC GAGTACGGTC TGGAGAAGAT CGTCCGTGAT CTGCGCGTGC ACCGGATCCT GGAGGGGACC AACGAGATCA TGCGGGTGGT CGTCGGCCGG GCCGAGGCCG CACGGTTTCG CGCTAGCGCA

Details for MyulA.01048.a.A1.GE29760

HARVESTED ON:	6/29/2010
SEQUENCED ON:	7/8/2010
EXPECTED MW:	43kDa
OBSERVED MW:	43kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Validated AA Sequence

MGTLEAQTQG PGSMFTLNDD ERVITQTAAA FADKRLAPYA LEWDATKHFP VDVLREAAEL GMAAIYCRED VGGSGLRRLD GVRIFEQLAI ADPVTAAFLS IHNMCTWMID SFGTDEQRKD WIPRLATMDA IASYCLTEPG AGSDASALSA RAVRQGSAPG SDYVLDGVKQ FISGAGASDV YVVMARTGAE GPRGISAFIV EKGTPGLSFG ALEEKMGWHA QPTAQVVLEG VRVPADAMLG GVDGTGAGFG IAMNGLNGGR LNIAACSLGG AQSAFAKTGA YVRERQAFGA SLLDEPTVRF TLADMATGLE ASRMLLWRAA NALDEDDADK VELCAMAKRF VTDTCFDVAD KALQLHGGYG YLREYGLEKI VRDLRVHRIL EGTNEIMRVV VGRAEAARFR

Validated NT Sequence

tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatgtttac cctcaacgac gacgagcggg tgatcaccca gaccgccgcc gccttcgccg ataagcggct ggccccctac gcgctggaat gggatgccac caagcacttc ccggtcgacg tgctgcgcga agccgccgaa ctgggaatgg cggcgatcta ctgccgagaa gacgtgggcg gtagcgggct gcgccggctc gacggcgtgc gcatcttcga acagctggcc atcgccgacc cggtcaccgc cgcgtttctg tccatccaca acatgtgcac ctggatgatc gacagcttcg gcaccgacga gcaacgcaag gactggattc cgcggctggc cacgatggac gccatcgcca gctactgcct gaccgagccg ggtgccggct ccgacgccag cgcgttgagc gcccgggcgg tgcggcaggg ttcggccccg gggtcggact atgtgctcga cggcgtcaag cagttcatct ccggtgcggg cgcctcggac gtctacgtgg tgatggcgcg caccggagcc gaggggccgc gcggcatctc ggcgttcatt gtcgaaaagg gcacgccggg gctgagtttc ggggcgctcg aagagaagat gggctggcac gcacagccca ccgcacaggt ggtgctcgaa ggagttcggg tgcctgccga cgcgatgctg ggcggcgttg acggaacggg cgccggattc ggcatcgcga tgaacggcct caacggtggc cgcctcaaca tcgcggcgtg ttcgctcggt ggcgcccagt ccgccttcgc caagacgggt gcctacgtgc gcgagcgcca ggcgttcggc gccagcctgc tcgacgagcc caccgtccgg ttcaccctgg ccgatatggc caccggtctg gaggcgtcgc gaatgctgtt gtggcgggcg gcaaacgcac tcgacgagga cgacgcggac aaggtggagc tgtgcgcgat ggccaagcgt tttgtcaccg acacctgctt cgacgtcgcc gacaaggctc tgcaactgca cggcggctac ggctacttgc gcgagtacgg tctggagaag atcgtccgtg atctgcgcgt gcaccggatc ctggagggga ccaacgagat catgcgggtg gtcgtcggcc gggccgaggc cgcacggttt cgcgct

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMFTLNDDER VITQTAAAFA DKRLAPYALE WDATKHFPVD VLREAAELGM AAIYCREDVG GSGLRRLDGV RIFEQLAIAD PVTAAFLSIH NMCTWMIDSF GTDEQRKDWI PRLATMDAIA SYCLTEPGAG SDASALSARA VRQGSAPGSD YVLDGVKQFI SGAGASDVYV VMARTGAEGP RGISAFIVEK GTPGLSFGAL EEKMGWHAQP TAQVVLEGVR VPADAMLGGV DGTGAGFGIA MNGLNGGRLN IAACSLGGAQ SAFAKTGAYV RERQAFGASL LDEPTVRFTL ADMATGLEAS RMLLWRAANA LDEDDADKVE LCAMAKRFVT DTCFDVADKA LQLHGGYGYL REYGLEKIVR DLRVHRILEG TNEIMRVVVG RAEAARFRAS A

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtttaccctc aacgacgacg agcgggtgat cacccagacc gccgccgcct tcgccgataa gcggctggcc ccctacgcgc tggaatggga tgccaccaag cacttcccgg tcgacgtgct gcgcgaagcc gccgaactgg gaatggcggc gatctactgc cgagaagacg tgggcggtag cgggctgcgc cggctcgacg gcgtgcgcat cttcgaacag ctggccatcg ccgacccggt caccgccgcg tttctgtcca tccacaacat gtgcacctgg atgatcgaca gcttcggcac cgacgagcaa cgcaaggact ggattccgcg gctggccacg atggacgcca tcgccagcta ctgcctgacc gagccgggtg ccggctccga cgccagcgcg ttgagcgccc gggcggtgcg gcagggttcg gccccggggt cggactatgt gctcgacggc gtcaagcagt tcatctccgg tgcgggcgcc tcggacgtct acgtggtgat ggcgcgcacc ggagccgagg ggccgcgcgg catctcggcg ttcattgtcg aaaagggcac gccggggctg agtttcgggg cgctcgaaga gaagatgggc tggcacgcac agcccaccgc acaggtggtg ctcgaaggag ttcgggtgcc tgccgacgcg atgctgggcg gcgttgacgg aacgggcgcc ggattcggca tcgcgatgaa cggcctcaac ggtggccgcc tcaacatcgc ggcgtgttcg ctcggtggcg cccagtccgc cttcgccaag acgggtgcct acgtgcgcga gcgccaggcg ttcggcgcca gcctgctcga cgagcccacc gtccggttca ccctggccga tatggccacc ggtctggagg cgtcgcgaat gctgttgtgg cgggcggcaa acgcactcga cgaggacgac gcggacaagg tggagctgtg cgcgatggcc aagcgttttg tcaccgacac ctgcttcgac gtcgccgaca aggctctgca actgcacggc ggctacggct acttgcgcga gtacggtctg gagaagatcg tccgtgatct gcgcgtgcac cggatcctgg aggggaccaa cgagatcatg cgggtggtcg tcggccgggc cgaggccgca cggtttcgcg ctagcgcaaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa

Details for MyulA.01048.a.A1.PS01033

PURIFICATION DATe:	4/6/2011
CONCENTRATION:	12.33mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	3
VIAL VOLUME:	50µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTTTACCCTC AACGACGACG AGCGGGTGAT CACCCAGACC GCCGCCGCCT TCGCCGATAA GCGGCTGGCC CCCTACGCGC TGGAATGGGA TGCCACCAAG CACTTCCCGG TCGACGTGCT GCGCGAAGCC GCCGAACTGG GAATGGCGGC GATCTACTGC CGAGAAGACG TGGGCGGTAG CGGGCTGCGC CGGCTCGACG GCGTGCGCAT CTTCGAACAG CTGGCCATCG CCGACCCGGT CACCGCCGCG TTTCTGTCCA TCCACAACAT GTGCACCTGG ATGATCGACA GCTTCGGCAC CGACGAGCAA CGCAAGGACT GGATTCCGCG GCTGGCCACG ATGGACGCCA TCGCCAGCTA CTGCCTGACC GAGCCGGGTG CCGGCTCCGA CGCCAGCGCG TTGAGCGCCC GGGCGGTGCG GCAGGGTTCG GCCCCGGGGT CGGACTATGT GCTCGACGGC GTCAAGCAGT TCATCTCCGG TGCGGGCGCC TCGGACGTCT ACGTGGTGAT GGCGCGCACC GGAGCCGAGG GGCCGCGCGG CATCTCGGCG TTCATTGTCG AAAAGGGCAC GCCGGGGCTG AGTTTCGGGG CGCTCGAAGA GAAGATGGGC TGGCACGCAC AGCCCACCGC ACAGGTGGTG CTCGAAGGAG TTCGGGTGCC TGCCGACGCG ATGCTGGGCG GCGTTGACGG AACGGGCGCC GGATTCGGCA TCGCGATGAA CGGCCTCAAC GGTGGCCGCC TCAACATCGC GGCGTGTTCG CTCGGTGGCG CCCAGTCCGC CTTCGCCAAG ACGGGTGCCT ACGTGCGCGA GCGCCAGGCG TTCGGCGCCA GCCTGCTCGA CGAGCCCACC GTCCGGTTCA CCCTGGCCGA TATGGCCACC GGTCTGGAGG CGTCGCGAAT GCTGTTGTGG CGGGCGGCAA ACGCACTCGA CGAGGACGAC GCGGACAAGG TGGAGCTGTG CGCGATGGCC AAGCGTTTTG TCACCGACAC CTGCTTCGAC GTCGCCGACA AGGCTCTGCA ACTGCACGGC GGCTACGGCT ACTTGCGCGA GTACGGTCTG GAGAAGATCG TCCGTGATCT GCGCGTGCAC CGGATCCTGG AGGGGACCAA CGAGATCATG CGGGTGGTCG TCGGCCGGGC CGAGGCCGCA CGGTTTCGCG CTAGCGCAAA ACAGCACGAA CAAGTTCTGC AGCCAAGCTT CTCGAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA