MymaA.01048.a

Acyl-CoA dehydrogenase, FadE9

CENTER ID: MymaA.01048.a
ORGANISM: Mycobacterium marinum ATCC BAA-535 / M
ASSOCIATED DISEASE: Cutaneous ulcerations, deep abscesses
CURRENT STATUS: diffraction
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MymaA.01048.a.A1.GE30098 Full length( MymaA.01048.a ) 1 390
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
External Resources
RESOURCE REFERENCE ID
OrthoMCL: OG5_136059
PATRIC ID: fig|216594.6.peg.1150
RefSeq: YP_001849389.1
UniProt: B2HCW2
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MFTLNDDERV ITQTAAAFAD KRLAPYALEW DATKHFPVDV LREAAELGMA AIYCREDVGG SGLRRLDGVR IFEQLAIADP VTAAFLSIHN MCAWMIDSFG TDEQRKDWIP RLATMDAIAS YCLTEPGAGS DASALSARAV RQGSAPGSDY VLDGVKQFIS GAGASDVYVV MARTGAEGPR GISAFIVEKG TPGLSFGALE EKMGWHAQPT AQVVLEGVRV PADAMLGGVD GTGAGFGIAM NGLNGGRLNI AACSLGGAQS AFAKAGAYVR ERQAFGASLL DEPTVRFTLA DMATGLEASR MLLWRAANAL DEDDADKVEL CAMAKRFVTD TCFDVADKAL QLHGGYGYLR EYGLEKIVRD LRVHRILEGT NEIMRVVVGR AEAARFRASA
NT Sequence
ATGTTTACCC TCAACGACGA CGAGCGGGTG ATCACCCAGA CCGCCGCCGC CTTCGCCGAT AAGCGGCTGG CCCCCTACGC GCTGGAATGG GATGCCACCA AGCACTTCCC GGTCGACGTG CTGCGCGAAG CCGCCGAACT GGGAATGGCG GCGATCTACT GCCGAGAAGA CGTGGGCGGT AGCGGGCTGC GCCGGCTCGA CGGCGTGCGC ATCTTCGAAC AGCTGGCCAT CGCCGACCCG GTCACCGCCG CGTTTCTGTC CATCCACAAC ATGTGCGCCT GGATGATCGA CAGCTTCGGC ACCGACGAGC AACGCAAGGA CTGGATTCCG CGGCTGGCCA CGATGGACGC CATCGCCAGC TACTGCCTGA CCGAGCCGGG TGCCGGCTCC GACGCCAGCG CGTTGAGCGC CCGGGCGGTG CGGCAGGGTT CGGCCCCGGG GTCGGACTAT GTGCTCGACG GCGTCAAGCA GTTCATCTCC GGTGCGGGCG CCTCGGACGT CTACGTGGTG ATGGCGCGCA CCGGAGCCGA GGGGCCGCGC GGCATCTCGG CGTTCATTGT CGAAAAGGGC ACGCCGGGGC TGAGTTTCGG GGCGCTCGAA GAGAAGATGG GCTGGCACGC ACAGCCCACC GCACAGGTGG TGCTCGAAGG AGTTCGGGTG CCCGCCGACG CGATGCTGGG CGGCGTTGAC GGAACGGGCG CCGGATTCGG CATCGCGATG AACGGCCTCA ACGGTGGCCG GCTCAACATC GCGGCGTGTT CGCTCGGTGG CGCCCAGTCC GCCTTCGCCA AGGCGGGTGC CTACGTGCGC GAGCGCCAGG CGTTCGGCGC CAGCCTGCTC GACGAGCCCA CCGTCCGGTT CACCCTGGCC GATATGGCCA CCGGTCTGGA GGCGTCGCGA ATGCTGTTGT GGCGGGCGGC AAACGCACTC GACGAGGACG ACGCGGACAA GGTGGAGCTG TGCGCGATGG CCAAGCGTTT TGTCACCGAC ACCTGCTTCG ACGTCGCCGA CAAGGCTCTG CAACTGCACG GCGGCTACGG CTACTTGCGC GAGTACGGTC TGGAGAAGAT CGTCCGTGAT CTGCGCGTGC ACCGGATCCT GGAGGGGACC AACGAGATCA TGCGGGTGGT CGTCGGCCGG GCCGAGGCCG CACGGTTTCG CGCTAGCGCA
Details for MymaA.01048.a.A1.GE30098
HARVESTED ON: 8/10/2010
SEQUENCED ON: 8/16/2010
EXPECTED MW: 43kDa
OBSERVED MW: 44kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 98
PERCENT COVERAGE: 53
Validated AA Sequence
MARTGAEGPR GISAFIVEKG TPGLSFGALE EKMGWHAQPT AQVVLEGVRV PADAMLGGVD GTGAGFGIAM NGLNGGRLNI AACSLGGAQS AFAKAGAYVR ERQAFGASLL DEPTVRFTLA DMATGLEASR MLLWRAANAL DEDDADKVEL CAMAKRFVXX XCFDVADKAL QLHGGYGYLR EYGLEKIVRD LRVHRILEGT NEIMRVVVGR AEAARFRA
Validated NT Sequence
gctagcgcga aaccgtgcgg cctcggcccg gccgacgacc acccgcatga tctcgttggt cccctccagg atccggtgca cgcgcagatc acggacgatc ttctccagac cgtactcgcg caagtagccg tagccgccgt gcagttgcag agccttgtcg gcgacgtcga agcaggngnn nntgacaaaa cgcttggcca tcgcgcacag ctccaccttg tccgcgtcgt cctcgtcgag tgcgtttgcc gcccgccaca acagcattcg cgacgcctcc agaccggtgg ccatatcggc cagggtgaac cggacggtgg gctcgtcgag caggctggcg ccgaacgcct ggcgctcgcg cacgtaggca cccgccttgg cgaaggcgga ctgggcgcca ccgagcgaac acgccgcgat gttgagccgg ccaccgttga ggccgttcat cgcgatgccg aatccggcgc ccgttccgtc aacgccgccc agcatcgcgt cggcgggcac ccgaactcct tcgagcacca cctgtgcggt gggctgtgcg tgccagccca tcttctcttc gagcgccccg aaactcagcc ccggcgtgcc cttttcgaca atgaacgccg agatgccgcg cggcccctcg gctccggtgc gcgccatcac cacgtagacg tccgaggcgc ccgcaccgga gatgaactgc ttgacgccgt cgagcacata gtccgacccc ggggccgaac cctgccgcac cgcccggggc gctcaacgcg ctggcgtcgg agccggcacc cgggctcggt caggcagtag ctggcgatgg cgtccatcgt ggccagccgc ggaatccagt ccttgcgttg ctcgtcggtg ccgaagctgt cgatcatcca ggcgcacatg ttgtggatgg acagaaacgc ggcggtgacc gggtcggcga tggccagctg ttcgaagatg cgcacgccgt cgagccggcg cagcccgcta ccgcccacgt cttctcggca gtagatcgcc gccattccca gttcggcggc ttcgcgcagc acgtcgaccg ggaagtgctt ggtggcatcc cattccagcg cgtagggggc cagccgctta tcggcgaagg cggcggcggt ctgggtgatc acccgctcgt cgtcgttgag ggtaaacatc gaaccaggac cctgggtctg agcttccagg gtacccata
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMFTLNDDER VITQTAAAFA DKRLAPYALE WDATKHFPVD VLREAAELGM AAIYCREDVG GSGLRRLDGV RIFEQLAIAD PVTAAFLSIH NMCAWMIDSF GTDEQRKDWI PRLATMDAIA SYCLTEPGAG SDASALSARA VRQGSAPGSD YVLDGVKQFI SGAGASDVYV VMARTGAEGP RGISAFIVEK GTPGLSFGAL EEKMGWHAQP TAQVVLEGVR VPADAMLGGV DGTGAGFGIA MNGLNGGRLN IAACSLGGAQ SAFAKAGAYV RERQAFGASL LDEPTVRFTL ADMATGLEAS RMLLWRAANA LDEDDADKVE LCAMAKRFVT DTCFDVADKA LQLHGGYGYL REYGLEKIVR DLRVHRILEG TNEIMRVVVG RAEAARFRAS
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtttaccctc aacgacgacg agcgggtgat cacccagacc gccgccgcct tcgccgataa gcggctggcc ccctacgcgc tggaatggga tgccaccaag cacttcccgg tcgacgtgct gcgcgaagcc gccgaactgg gaatggcggc gatctactgc cgagaagacg tgggcggtag cgggctgcgc cggctcgacg gcgtgcgcat cttcgaacag ctggccatcg ccgacccggt caccgccgcg tttctgtcca tccacaacat gtgcgcctgg atgatcgaca gcttcggcac cgacgagcaa cgcaaggact ggattccgcg gctggccacg atggacgcca tcgccagcta ctgcctgacc gagccgggtg ccggctccga cgccagcgcg ttgagcgccc gggcggtgcg gcagggttcg gccccggggt cggactatgt gctcgacggc gtcaagcagt tcatctccgg tgcgggcgcc tcggacgtct acgtggtgat ggcgcgcacc ggagccgagg ggccgcgcgg catctcggcg ttcattgtcg aaaagggcac gccggggctg agtttcgggg cgctcgaaga gaagatgggc tggcacgcac agcccaccgc acaggtggtg ctcgaaggag ttcgggtgcc cgccgacgcg atgctgggcg gcgttgacgg aacgggcgcc ggattcggca tcgcgatgaa cggcctcaac ggtggccggc tcaacatcgc ggcgtgttcg ctcggtggcg cccagtccgc cttcgccaag gcgggtgcct acgtgcgcga gcgccaggcg ttcggcgcca gcctgctcga cgagcccacc gtccggttca ccctggccga tatggccacc ggtctggagg cgtcgcgaat gctgttgtgg cgggcggcaa acgcactcga cgaggacgac gcggacaagg tggagctgtg cgcgatggcc aagcgttttg tcaccgacac ctgcttcgac gtcgccgaca aggctctgca actgcacggc ggctacggct acttgcgcga gtacggtctg gagaagatcg tccgtgatct gcgcgtgcac cggatcctgg aggggaccaa cgagatcatg cgggtggtcg tcggccgggc cgaggccgca cggtttcgcg ctagcaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa