MypaA.01048.a

FadE9

CENTER ID: MypaA.01048.a
ORGANISM: Mycobacterium paratuberculosis ATCC BAA-968 / K-10
ASSOCIATED DISEASE: Johne's disease
CURRENT STATUS: diffraction
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MypaA.01048.a.A1.GE29532 Full length( MypaA.01048.a ) 1 386
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MypaA.01048.a.A1.PW29430 Full length( MypaA.01048.a ) 1 386

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|262316.17.peg.4486
RefSeq: NP_963148.1
UniProt: Q73S62

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MFTLDDDERV IAETAAAFAA KRLAPHALEW DAAEHFPVDV LRESAELGMA AIYCREDVGG SGLRRLDGVR IFEQLAIADP TTAAFLSIHN MCAWMIDSYG TPEQRKAWVP RLAAMDVIAS YCLTEPGAGS DAAALSTRAV RQGGDYVLDG VKQFISGAGA SDVYLVMART GGEGPRGISA FVVEKGSAGL SFGSPEEKMG WHAQPTAQVI LEGVRVPADA MLGGAEGEGA GFGIAMNGLN GGRLNIAACS LGGAQAAFDK AGAYVRDRQA FGSPLLDEPT IRFALADMAT GLETSRMMLW RAAGALDADD PDKVELCAMA KRYVTDTCFE VADKALQLHG GYGYLREYGL EKIVRDLRVH RILEGTNEIM RVVIGRAEAA RFRATA
NT Sequence
ATGTTTACCC TGGACGACGA CGAGCGGGTG ATCGCCGAGA CGGCCGCCGC GTTCGCCGCC AAGCGCCTGG CCCCGCACGC GCTGGAATGG GATGCGGCCG AACACTTTCC GGTTGACGTG CTGCGCGAGT CGGCCGAGCT CGGGATGGCG GCGATCTACT GCCGCGAGGA CGTCGGCGGC AGCGGGCTGC GCCGGCTGGA CGGGGTCCGC ATCTTCGAGC AGCTGGCCAT CGCCGACCCC ACCACGGCCG CGTTCCTGTC CATCCACAAC ATGTGCGCGT GGATGATCGA CAGCTACGGC ACGCCCGAAC AGCGCAAGGC CTGGGTGCCG CGGCTGGCGG CGATGGACGT CATCGCCAGC TACTGCCTGA CCGAGCCGGG CGCGGGGTCC GACGCCGCCG CGCTGAGCAC CCGCGCGGTC AGACAGGGCG GCGATTACGT GCTGGACGGC GTCAAGCAGT TCATCTCGGG CGCCGGGGCC TCCGACGTCT ACCTGGTGAT GGCCCGCACG GGCGGTGAAG GGCCGCGCGG CATCTCCGCG TTCGTCGTCG AAAAGGGTTC TGCTGGGCTG AGTTTCGGTT CGCCCGAGGA GAAGATGGGC TGGCACGCCC AACCCACCGC GCAGGTGATC CTGGAGGGGG TGCGGGTGCC CGCCGACGCG ATGCTGGGCG GCGCCGAGGG TGAGGGCGCC GGATTCGGCA TCGCGATGAA CGGCCTCAAC GGCGGCCGGC TCAACATCGC CGCGTGCTCG CTGGGCGGCG CGCAGGCGGC CTTCGACAAG GCCGGGGCCT ACGTGCGCGA TCGGCAGGCG TTCGGGTCGC CGCTGCTCGA CGAGCCGACC ATCCGGTTCG CCCTGGCCGA CATGGCCACC GGCCTGGAAA CGTCGCGAAT GATGTTGTGG CGGGCCGCCG GTGCGCTGGA CGCCGACGAT CCCGACAAGG TCGAGTTGTG CGCGATGGCC AAACGCTACG TCACCGACAC CTGCTTCGAG GTCGCCGACA AGGCGCTGCA GCTGCACGGC GGCTACGGCT ATCTGCGCGA GTATGGTCTG GAGAAGATCG TGCGCGACCT GCGGGTGCAT CGCATCCTGG AGGGGACCAA CGAGATCATG CGGGTGGTCA TCGGCCGGGC CGAAGCGGCG CGGTTCCGGG CCACCGCA
Details for MypaA.01048.a.A1.GE29532
HARVESTED ON: 6/15/2010
SEQUENCED ON: 7/8/2010
EXPECTED MW: 43kDa
OBSERVED MW: 42kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMFTLDDDER VIAETAAAFA AKRLAPHALE WDAAEHFPVD VLRESAELGM AAIYCREDVG GSGLRRLDGV RIFEQLAIAD PTTAAFLSIH NMCAWMIDSY GTPEQRKAWV PRLAAMDVIA SYCLTEPGAG SDAAALSTRA VRQGGDYVLD GVKQFISGAG ASDVYLVMAR TGGEGPRGIS AFVVEKGSAG LSFGSPEEKM GWHAQPTAQV ILEGVRVPAD AMLGGAEGEG AGFGIAMNGL NGGRLNIAAC SLGGAQAAFD KAGAYVRDRQ AFGSPLLDEP TIRFALADMA TGLETSRMML WRAAGALDAD DPDKVELCAM AKRYVTDTCF EVADKALQLH GGYGYLREYG LEKIVRDLRV HRILEGTNEI MRVVIGRAEA ARFRATA
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgttta ccctggacga cgacgagcgg gtgatcgccg agacggccgc cgcgttcgcc gccaagcgcc tggccccgca cgcgctggaa tgggatgcgg ccgaacactt tccggttgac gtgctgcgcg agtcggccga gctcgggatg gcggcgatct actgccgcga ggacgtcggc ggcagcgggc tgcgccggct ggacggggtc cgcatcttcg agcagctggc catcgccgac cccaccacgg ccgcgttcct gtccatccac aacatgtgcg cgtggatgat cgacagctac ggcacgcccg aacagcgcaa ggcctgggtg ccgcggctgg cggcgatgga cgtcatcgcc agctactgcc tgaccgagcc gggcgcgggg tccgacgccg ccgcgctgag cacccgcgcg gtcagacagg gcggcgatta cgtgctggac ggcgtcaagc agttcatctc gggcgccggg gcctccgacg tctacctggt gatggcccgc acgggcggtg aagggccgcg cggcatctcc gcgttcgtcg tcgaaaaggg ttctgctggg ctgagtttcg gttcgcccga ggagaagatg ggctggcacg cccaacccac cgcgcaggtg atcctggagg gggtgcgggt gcccgccgac gcgatgctgg gcggcgccga gggtgagggc gccggattcg gcatcgcgat gaacggcctc aacggcggcc ggctcaacat cgccgcgtgc tcgctgggcg gcgcgcaggc ggccttcgac aaggccgggg cctacgtgcg cgatcggcag gcgttcgggt cgccgctgct cgacgagccg accatccggt tcgccctggc cgacatggcc accggcctgg aaacgtcgcg aatgatgttg tggcgggccg ccggtgcgct ggacgccgac gatcccgaca aggtcgagtt gtgcgcgatg gccaaacgct acgtcaccga cacctgcttc gaggtcgccg acaaggcgct gcagctgcac ggcggctacg gctatctgcg cgagtatggt ctggagaaga tcgtgcgcga cctgcgggtg catcgcatcc tggaggggac caacgagatc atgcgggtgg tcatcggccg ggccgaagcg gcgcggttcc gggccaccgc ataataa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMFTLDDDER VIAETAAAFA AKRLAPHALE WDAAEHFPVD VLRESAELGM AAIYCREDVG GSGLRRLDGV RIFEQLAIAD PTTAAFLSIH NMCAWMIDSY GTPEQRKAWV PRLAAMDVIA SYCLTEPGAG SDAAALSTRA VRQGGDYVLD GVKQFISGAG ASDVYLVMAR TGGEGPRGIS AFVVEKGSAG LSFGSPEEKM GWHAQPTAQV ILEGVRVPAD AMLGGAEGEG AGFGIAMNGL NGGRLNIAAC SLGGAQAAFD KAGAYVRDRQ AFGSPLLDEP TIRFALADMA TGLETSRMML WRAAGALDAD DPDKVELCAM AKRYVTDTCF EVADKALQLH GGYGYLREYG LEKIVRDLRV HRILEGTNEI MRVVIGRAEA ARFRATA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtttaccctg gacgacgacg agcgggtgat cgccgagacg gccgccgcgt tcgccgccaa gcgcctggcc ccgcacgcgc tggaatggga tgcggccgaa cactttccgg ttgacgtgct gcgcgagtcg gccgagctcg ggatggcggc gatctactgc cgcgaggacg tcggcggcag cgggctgcgc cggctggacg gggtccgcat cttcgagcag ctggccatcg ccgaccccac cacggccgcg ttcctgtcca tccacaacat gtgcgcgtgg atgatcgaca gctacggcac gcccgaacag cgcaaggcct gggtgccgcg gctggcggcg atggacgtca tcgccagcta ctgcctgacc gagccgggcg cggggtccga cgccgccgcg ctgagcaccc gcgcggtcag acagggcggc gattacgtgc tggacggcgt caagcagttc atctcgggcg ccggggcctc cgacgtctac ctggtgatgg cccgcacggg cggtgaaggg ccgcgcggca tctccgcgtt cgtcgtcgaa aagggttctg ctgggctgag tttcggttcg cccgaggaga agatgggctg gcacgcccaa cccaccgcgc aggtgatcct ggagggggtg cgggtgcccg ccgacgcgat gctgggcggc gccgagggtg agggcgccgg attcggcatc gcgatgaacg gcctcaacgg cggccggctc aacatcgccg cgtgctcgct gggcggcgcg caggcggcct tcgacaaggc cggggcctac gtgcgcgatc ggcaggcgtt cgggtcgccg ctgctcgacg agccgaccat ccggttcgcc ctggccgaca tggccaccgg cctggaaacg tcgcgaatga tgttgtggcg ggccgccggt gcgctggacg ccgacgatcc cgacaaggtc gagttgtgcg cgatggccaa acgctacgtc accgacacct gcttcgaggt cgccgacaag gcgctgcagc tgcacggcgg ctacggctat ctgcgcgagt atggtctgga gaagatcgtg cgcgacctgc gggtgcatcg catcctggag gggaccaacg agatcatgcg ggtggtcatc ggccgggccg aagcggcgcg gttccgggcc accgcaaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa
Details for MypaA.01048.a.A1.PW29430
PURIFICATION DATe: 8/16/2010
CONCENTRATION: 30.04mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 11
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMFTLDDDER VIAETAAAFA AKRLAPHALE WDAAEHFPVD VLRESAELGM AAIYCREDVG GSGLRRLDGV RIFEQLAIAD PTTAAFLSIH NMCAWMIDSY GTPEQRKAWV PRLAAMDVIA SYCLTEPGAG SDAAALSTRA VRQGGDYVLD GVKQFISGAG ASDVYLVMAR TGGEGPRGIS AFVVEKGSAG LSFGSPEEKM GWHAQPTAQV ILEGVRVPAD AMLGGAEGEG AGFGIAMNGL NGGRLNIAAC SLGGAQAAFD KAGAYVRDRQ AFGSPLLDEP TIRFALADMA TGLETSRMML WRAAGALDAD DPDKVELCAM AKRYVTDTCF EVADKALQLH GGYGYLREYG LEKIVRDLRV HRILEGTNEI MRVVIGRAEA ARFRATA
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgttta ccctggacga cgacgagcgg gtgatcgccg agacggccgc cgcgttcgcc gccaagcgcc tggccccgca cgcgctggaa tgggatgcgg ccgaacactt tccggttgac gtgctgcgcg agtcggccga gctcgggatg gcggcgatct actgccgcga ggacgtcggc ggcagcgggc tgcgccggct ggacggggtc cgcatcttcg agcagctggc catcgccgac cccaccacgg ccgcgttcct gtccatccac aacatgtgcg cgtggatgat cgacagctac ggcacgcccg aacagcgcaa ggcctgggtg ccgcggctgg cggcgatgga cgtcatcgcc agctactgcc tgaccgagcc gggcgcgggg tccgacgccg ccgcgctgag cacccgcgcg gtcagacagg gcggcgatta cgtgctggac ggcgtcaagc agttcatctc gggcgccggg gcctccgacg tctacctggt gatggcccgc acgggcggtg aagggccgcg cggcatctcc gcgttcgtcg tcgaaaaggg ttctgctggg ctgagtttcg gttcgcccga ggagaagatg ggctggcacg cccaacccac cgcgcaggtg atcctggagg gggtgcgggt gcccgccgac gcgatgctgg gcggcgccga gggtgagggc gccggattcg gcatcgcgat gaacggcctc aacggcggcc ggctcaacat cgccgcgtgc tcgctgggcg gcgcgcaggc ggccttcgac aaggccgggg cctacgtgcg cgatcggcag gcgttcgggt cgccgctgct cgacgagccg accatccggt tcgccctggc cgacatggcc accggcctgg aaacgtcgcg aatgatgttg tggcgggccg ccggtgcgct ggacgccgac gatcccgaca aggtcgagtt gtgcgcgatg gccaaacgct acgtcaccga cacctgcttc gaggtcgccg acaaggcgct gcagctgcac ggcggctacg gctatctgcg cgagtatggt ctggagaaga tcgtgcgcga cctgcgggtg catcgcatcc tggaggggac caacgagatc atgcgggtgg tcatcggccg ggccgaagcg gcgcggttcc gggccaccgc ataataa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMFTLDDDER VIAETAAAFA AKRLAPHALE WDAAEHFPVD VLRESAELGM AAIYCREDVG GSGLRRLDGV RIFEQLAIAD PTTAAFLSIH NMCAWMIDSY GTPEQRKAWV PRLAAMDVIA SYCLTEPGAG SDAAALSTRA VRQGGDYVLD GVKQFISGAG ASDVYLVMAR TGGEGPRGIS AFVVEKGSAG LSFGSPEEKM GWHAQPTAQV ILEGVRVPAD AMLGGAEGEG AGFGIAMNGL NGGRLNIAAC SLGGAQAAFD KAGAYVRDRQ AFGSPLLDEP TIRFALADMA TGLETSRMML WRAAGALDAD DPDKVELCAM AKRYVTDTCF EVADKALQLH GGYGYLREYG LEKIVRDLRV HRILEGTNEI MRVVIGRAEA ARFRATA
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTTTACCCTG GACGACGACG AGCGGGTGAT CGCCGAGACG GCCGCCGCGT TCGCCGCCAA GCGCCTGGCC CCGCACGCGC TGGAATGGGA TGCGGCCGAA CACTTTCCGG TTGACGTGCT GCGCGAGTCG GCCGAGCTCG GGATGGCGGC GATCTACTGC CGCGAGGACG TCGGCGGCAG CGGGCTGCGC CGGCTGGACG GGGTCCGCAT CTTCGAGCAG CTGGCCATCG CCGACCCCAC CACGGCCGCG TTCCTGTCCA TCCACAACAT GTGCGCGTGG ATGATCGACA GCTACGGCAC GCCCGAACAG CGCAAGGCCT GGGTGCCGCG GCTGGCGGCG ATGGACGTCA TCGCCAGCTA CTGCCTGACC GAGCCGGGCG CGGGGTCCGA CGCCGCCGCG CTGAGCACCC GCGCGGTCAG ACAGGGCGGC GATTACGTGC TGGACGGCGT CAAGCAGTTC ATCTCGGGCG CCGGGGCCTC CGACGTCTAC CTGGTGATGG CCCGCACGGG CGGTGAAGGG CCGCGCGGCA TCTCCGCGTT CGTCGTCGAA AAGGGTTCTG CTGGGCTGAG TTTCGGTTCG CCCGAGGAGA AGATGGGCTG GCACGCCCAA CCCACCGCGC AGGTGATCCT GGAGGGGGTG CGGGTGCCCG CCGACGCGAT GCTGGGCGGC GCCGAGGGTG AGGGCGCCGG ATTCGGCATC GCGATGAACG GCCTCAACGG CGGCCGGCTC AACATCGCCG CGTGCTCGCT GGGCGGCGCG CAGGCGGCCT TCGACAAGGC CGGGGCCTAC GTGCGCGATC GGCAGGCGTT CGGGTCGCCG CTGCTCGACG AGCCGACCAT CCGGTTCGCC CTGGCCGACA TGGCCACCGG CCTGGAAACG TCGCGAATGA TGTTGTGGCG GGCCGCCGGT GCGCTGGACG CCGACGATCC CGACAAGGTC GAGTTGTGCG CGATGGCCAA ACGCTACGTC ACCGACACCT GCTTCGAGGT CGCCGACAAG GCGCTGCAGC TGCACGGCGG CTACGGCTAT CTGCGCGAGT ATGGTCTGGA GAAGATCGTG CGCGACCTGC GGGTGCATCG CATCCTGGAG GGGACCAACG AGATCATGCG GGTGGTCATC GGCCGGGCCG AAGCGGCGCG GTTCCGGGCC ACCGCAAAAC AGCACGAACA AGTTCTGCAG CCAAGCTTCT CGAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAA