SSGCID: Target Page

MytuD.00897.a

glucose-6-phosphate 1-dehydrogenase

CENTER ID:	MytuD.00897.a
ORGANISM:	Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE:	Tuberculosis
CURRENT STATUS:	crystallized
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MytuD.00897.a.A1.GE25915	Full length( MytuD.00897.a )		1	466

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MytuD.00897.a.A1.PS00297	Full length( MytuD.00897.a )		1	466

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|83332.12.peg.1255
RefSeq:	YP_177789.1
TubercuList:	Rv1121
MTB Network Portal:	Rv1121
UniProt:	P9WN71

Orthologues for Rv1121

Species	% Identity	% Coverage	Clones	Proteins	Structures
ulcerans	83	72	1	0	0
marinum	81	100	1	1	0
avium	79	100	1	1	1
paratuberculosis	78	100	0	0	0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MVDGGGGASD LLVIFGITGD LARKMTFRAL YRLERHQLLD CPILGVASDD MSVGQLVKWA RESIGRTEKI DDAVFDRLAG RLSYLHGDVT DSQLYDSLAE LIGSACRPLY YLEMPPALFA PIVENLANVR LLERARVAVE KPFGHDLASA LELNARLRAV LGEDQILRVD HFLGKQPVVE LEYLRFANQA LAELWDRNSI SEIHITMAED FGVEDRGKFY DAVGALRDVV QNHLLQVLAL VTMEPPVGSS ADDLNDKKAE VFRAMAPLDP DRCVRGQYLG YTEVAGVASD SATETYVALR TEIDNWRWAG VPIFVRAGKE LPAKVTEVRL FLRRVPALAF LPNRRPAEPN QIVLRIDPDP GMRLQISAHT DDSWRDIHLD SSFAVDLGEP IRPYERLLYA GLVGDHQLFA REDSIEQTWR IVQPLLDNPG EIHRYDRGSW GPEAAQSLLR GHRGWQSPWL PRGTDA

NT Sequence

TTGGTTGACG GCGGAGGTGG CGCGTCAGAT CTGTTGGTGA TCTTCGGAAT TACCGGTGAC CTGGCCCGCA AGATGACCTT CCGCGCGTTG TATCGGCTCG AGCGCCACCA GTTGCTGGAC TGCCCCATCC TGGGTGTGGC CAGTGACGAC ATGTCCGTCG GGCAGTTGGT CAAGTGGGCT CGCGAGTCCA TCGGTCGTAC CGAAAAGATC GACGATGCGG TGTTCGACCG GTTGGCGGGC CGGTTGTCCT ACCTGCACGG TGACGTCACC GACAGCCAGC TCTACGATTC GCTGGCCGAA CTGATTGGCT CGGCCTGTCG GCCGCTGTAT TACCTGGAAA TGCCGCCGGC GCTGTTCGCG CCGATTGTCG AAAATCTCGC GAACGTGCGG CTGTTGGAGC GCGCACGCGT TGCCGTGGAA AAGCCGTTCG GCCACGACCT GGCCTCCGCG CTCGAACTCA ACGCCCGGCT GCGAGCGGTG TTGGGCGAAG ACCAAATCCT GCGTGTGGAC CACTTTCTGG GCAAGCAGCC CGTCGTCGAG CTGGAGTACC TGAGGTTCGC CAATCAGGCG TTAGCCGAGC TCTGGGATCG CAACAGCATC TCCGAGATCC ACATCACCAT GGCCGAGGAC TTCGGGGTGG AGGACCGCGG CAAGTTTTAC GACGCCGTCG GTGCCCTGCG TGACGTCGTG CAAAACCATC TGCTGCAGGT GCTGGCGCTG GTGACGATGG AACCGCCGGT CGGTTCCAGC GCCGATGACC TCAACGACAA GAAGGCCGAG GTCTTCCGGG CGATGGCGCC GCTGGATCCC GATCGGTGCG TGCGTGGGCA GTACCTCGGC TACACCGAAG TTGCGGGCGT AGCAAGCGAT TCGGCGACCG AAACGTATGT CGCGCTGCGA ACCGAGATCG ACAACTGGCG CTGGGCCGGG GTGCCGATCT TCGTGCGGGC CGGAAAAGAG CTGCCCGCGA AGGTCACCGA AGTACGGCTA TTTTTACGCC GAGTTCCGGC ATTGGCCTTT CTGCCCAACC GCCGACCGGC CGAGCCCAAC CAGATTGTGC TGCGTATCGA CCCCGATCCG GGTATGCGAC TGCAGATTTC GGCCCACACC GACGACTCGT GGCGAGATAT CCACCTGGAC TCCTCGTTCG CGGTGGACCT CGGTGAACCG ATACGACCCT ATGAGCGGCT GCTGTATGCC GGATTGGTCG GCGATCACCA GTTGTTCGCC CGCGAGGACA GCATCGAGCA GACGTGGCGG ATCGTGCAGC CGCTGCTCGA CAACCCGGGT GAAATCCATC GGTACGATCG CGGTTCCTGG GGTCCGGAAG CCGCGCAGTC GTTGCTGCGC GGTCACCGCG GTTGGCAGTC GCCGTGGCTG CCCCGCGGCA CGGACGCA

Details for MytuD.00897.a.A1.GE25915

HARVESTED ON:	1/9/2009
SEQUENCED ON:	8/18/2010
EXPECTED MW:	54kDa
OBSERVED MW:	54kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with sequence variation
PERCENT IDENTITY:	99
PERCENT COVERAGE:	97

Validated AA Sequence

MGTLEAQTQG PGSMLVDGGG GASDLLVIFG ITGDLARKMT FRALYRLERH QLLDCPILGV ASDDMSVGQL VKWARESIGR TEKIDDAVFD RLAGRLSYLH GDVTDSQLYD SLAELIGSAC RPLYYLEMPP ALFAPIVENL ANVRLLERAR VAVEKPFGHD LASALELNAR LRAVLGEDQI LRVDHFLGKQ PVVXLEYLRF ANQALAELWD RNSISEIHIT MAEDFGVEDR GKFYDAVGAL RDVVQNHLLQ VLALVTMEPP VGSSADDLND KKAEVFRAMA PLDPDRCVRG QYLGYTEVAG VASDSATETY VALRTEIDNW RWAGVPIFVR AGKELPAKVT EVRLFLRRVP ALAFLPNRRP AEPNQIVLRI DPDPGMRLQI SAHTDDSWRD IHLDSSFAVD LGEPIRPYER LLYAGLVGDH QLFAREDSIE QTWRIVQPLL DNPGEIHRYD RGSWGPEAAQ SLLRGHRGWQ SPWLPRGT

Validated NT Sequence

ataccntggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gttggttgac ggcggaggtg gcgcgtcaga tctgttggtg atcttcggaa ttaccggtga cctggcccgc aagatgacct tccgcgcgtt gtatcggctc gagcgccacc agttgctgga ctgccccatc ctgggtgtgg ccagtgacga catgtccgtc gggcagttgg tcaagtgggc tcgcgagtcc atcggtcgta ccgaaaagat cgacgatgcg gtgttcgacc ggttggcggg ccggttgtcc tacctgcacg gtgacgtcac cgacagccag ctctacgatt cgctggccga actgattggc tcggcctgtc ggccgctgta ttacctggaa atgccgccgg cgctgttcgc gccgattgtc gaaaatctcg cgaacgtgcg gctgttggag cgcgcacgcg ttgccgtgga aaagccgttc ggccacgacc tggcctccgc gctcgaactc aacgcccggc tgcgagcggt gttgggcgaa gaccaaatcc tgcgtgtgga ccactttctg ggcaagcagc ccgtcgtcna gctggagtac ctgaggttcg ccaatcaggc gttagccgag ctctgggatc gcaacagcat ctccgagatc cacatcacca tggccgagga cttcggggtg gaggaccgcg gcaagtttta cgacgccgtc ggtgccctgc gtgacgtcgt gcaaaaccat ctgctgcagg tgctggcgct ggtgacgatg gaaccgccgg tcggttccag cgccgatgac ctcaacgaca agaaggccga ggtcttccgg gcgatggcgc cgctggatcc cgatcggtgc gtgcgtgggc agtacctcgg ctacaccgaa gttgcgggcg tagcaagcga ttcggcgacc gaaacgtatg tcgcgctgcg aaccgagatc gacaactggc gctgggccgg ggtgccgatc ttcgtgcggg ccggaaaaga gctgcccgcg aaggtcaccg aagtacggct atttctacgc cgagttccgg cattggcctt tctgcccaac cgccgaccgg ccgagcccaa ccagattgtg ctgcgtatcg accccgatcc gggtatgcga ctgcagattt cggcccacac cgacgactcg tggcgagata tccacctgga ctcctcgttc gcggtggacc tcggtgaacc gatacgaccc tatgagcggc tgctgtatgc cggattggtc ggcgatcacc agttgttcgc ccgcgaggac agcatcgagc agacgtggcg gatcgtgcag ccgctgctcg acaacccggg tgaaatccat cggtacgatc gcggttcctg gggtccggaa gccgcgcagt cgttgctgcg cggtcaccgc ggttggcagt cgccgtggct gccccgcggc acgg

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMLVDGGGGA SDLLVIFGIT GDLARKMTFR ALYRLERHQL LDCPILGVAS DDMSVGQLVK WARESIGRTE KIDDAVFDRL AGRLSYLHGD VTDSQLYDSL AELIGSACRP LYYLEMPPAL FAPIVENLAN VRLLERARVA VEKPFGHDLA SALELNARLR AVLGEDQILR VDHFLGKQPV VELEYLRFAN QALAELWDRN SISEIHITMA EDFGVEDRGK FYDAVGALRD VVQNHLLQVL ALVTMEPPVG SSADDLNDKK AEVFRAMAPL DPDRCVRGQY LGYTEVAGVA SDSATETYVA LRTEIDNWRW AGVPIFVRAG KELPAKVTEV RLFLRRVPAL AFLPNRRPAE PNQIVLRIDP DPGMRLQISA HTDDSWRDIH LDSSFAVDLG EPIRPYERLL YAGLVGDHQL FAREDSIEQT WRIVQPLLDN PGEIHRYDRG SWGPEAAQSL LRGHRGWQSP WLPRGTDA

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gttggttgac ggcggaggtg gcgcgtcaga tctgttggtg atcttcggaa ttaccggtga cctggcccgc aagatgacct tccgcgcgtt gtatcggctc gagcgccacc agttgctgga ctgccccatc ctgggtgtgg ccagtgacga catgtccgtc gggcagttgg tcaagtgggc tcgcgagtcc atcggtcgta ccgaaaagat cgacgatgcg gtgttcgacc ggttggcggg ccggttgtcc tacctgcacg gtgacgtcac cgacagccag ctctacgatt cgctggccga actgattggc tcggcctgtc ggccgctgta ttacctggaa atgccgccgg cgctgttcgc gccgattgtc gaaaatctcg cgaacgtgcg gctgttggag cgcgcacgcg ttgccgtgga aaagccgttc ggccacgacc tggcctccgc gctcgaactc aacgcccggc tgcgagcggt gttgggcgaa gaccaaatcc tgcgtgtgga ccactttctg ggcaagcagc ccgtcgtcga gctggagtac ctgaggttcg ccaatcaggc gttagccgag ctctgggatc gcaacagcat ctccgagatc cacatcacca tggccgagga cttcggggtg gaggaccgcg gcaagtttta cgacgccgtc ggtgccctgc gtgacgtcgt gcaaaaccat ctgctgcagg tgctggcgct ggtgacgatg gaaccgccgg tcggttccag cgccgatgac ctcaacgaca agaaggccga ggtcttccgg gcgatggcgc cgctggatcc cgatcggtgc gtgcgtgggc agtacctcgg ctacaccgaa gttgcgggcg tagcaagcga ttcggcgacc gaaacgtatg tcgcgctgcg aaccgagatc gacaactggc gctgggccgg ggtgccgatc ttcgtgcggg ccggaaaaga gctgcccgcg aaggtcaccg aagtacggct atttttacgc cgagttccgg cattggcctt tctgcccaac cgccgaccgg ccgagcccaa ccagattgtg ctgcgtatcg accccgatcc gggtatgcga ctgcagattt cggcccacac cgacgactcg tggcgagata tccacctgga ctcctcgttc gcggtggacc tcggtgaacc gatacgaccc tatgagcggc tgctgtatgc cggattggtc ggcgatcacc agttgttcgc ccgcgaggac agcatcgagc agacgtggcg gatcgtgcag ccgctgctcg acaacccggg tgaaatccat cggtacgatc gcggttcctg gggtccggaa gccgcgcagt cgttgctgcg cggtcaccgc ggttggcagt cgccgtggct gccccgcggc acggacgcaa aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a

Details for MytuD.00897.a.A1.PS00297

PURIFICATION DATe:	4/15/2009
CONCENTRATION:	6.2mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	3
VIAL VOLUME:	200µl
PERCENT IDENTITY:	99
PERCENT COVERAGE:	97

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTTGGTTGAC GGCGGAGGTG GCGCGTCAGA TCTGTTGGTG ATCTTCGGAA TTACCGGTGA CCTGGCCCGC AAGATGACCT TCCGCGCGTT GTATCGGCTC GAGCGCCACC AGTTGCTGGA CTGCCCCATC CTGGGTGTGG CCAGTGACGA CATGTCCGTC GGGCAGTTGG TCAAGTGGGC TCGCGAGTCC ATCGGTCGTA CCGAAAAGAT CGACGATGCG GTGTTCGACC GGTTGGCGGG CCGGTTGTCC TACCTGCACG GTGACGTCAC CGACAGCCAG CTCTACGATT CGCTGGCCGA ACTGATTGGC TCGGCCTGTC GGCCGCTGTA TTACCTGGAA ATGCCGCCGG CGCTGTTCGC GCCGATTGTC GAAAATCTCG CGAACGTGCG GCTGTTGGAG CGCGCACGCG TTGCCGTGGA AAAGCCGTTC GGCCACGACC TGGCCTCCGC GCTCGAACTC AACGCCCGGC TGCGAGCGGT GTTGGGCGAA GACCAAATCC TGCGTGTGGA CCACTTTCTG GGCAAGCAGC CCGTCGTCGA GCTGGAGTAC CTGAGGTTCG CCAATCAGGC GTTAGCCGAG CTCTGGGATC GCAACAGCAT CTCCGAGATC CACATCACCA TGGCCGAGGA CTTCGGGGTG GAGGACCGCG GCAAGTTTTA CGACGCCGTC GGTGCCCTGC GTGACGTCGT GCAAAACCAT CTGCTGCAGG TGCTGGCGCT GGTGACGATG GAACCGCCGG TCGGTTCCAG CGCCGATGAC CTCAACGACA AGAAGGCCGA GGTCTTCCGG GCGATGGCGC CGCTGGATCC CGATCGGTGC GTGCGTGGGC AGTACCTCGG CTACACCGAA GTTGCGGGCG TAGCAAGCGA TTCGGCGACC GAAACGTATG TCGCGCTGCG AACCGAGATC GACAACTGGC GCTGGGCCGG GGTGCCGATC TTCGTGCGGG CCGGAAAAGA GCTGCCCGCG AAGGTCACCG AAGTACGGCT ATTTTTACGC CGAGTTCCGG CATTGGCCTT TCTGCCCAAC CGCCGACCGG CCGAGCCCAA CCAGATTGTG CTGCGTATCG ACCCCGATCC GGGTATGCGA CTGCAGATTT CGGCCCACAC CGACGACTCG TGGCGAGATA TCCACCTGGA CTCCTCGTTC GCGGTGGACC TCGGTGAACC GATACGACCC TATGAGCGGC TGCTGTATGC CGGATTGGTC GGCGATCACC AGTTGTTCGC CCGCGAGGAC AGCATCGAGC AGACGTGGCG GATCGTGCAG CCGCTGCTCG ACAACCCGGG TGAAATCCAT CGGTACGATC GCGGTTCCTG GGGTCCGGAA GCCGCGCAGT CGTTGCTGCG CGGTCACCGC GGTTGGCAGT CGCCGTGGCT GCCCCGCGGC ACGGACGCAA AACAGCACGA ACAAGTTCTG CAGCCAAGCT TCTCGAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA A