MymaA.00897.a

Glucose-6-phosphate 1-dehydrogenase

CENTER ID: MymaA.00897.a
ORGANISM: Mycobacterium marinum ATCC BAA-535 / M
ASSOCIATED DISEASE: Cutaneous ulcerations, deep abscesses
CURRENT STATUS: crystallized
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MymaA.00897.a.A1.GE30084 Full length( MymaA.00897.a ) 1 469
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MymaA.00897.a.A1.PS00980 Full length( MymaA.00897.a ) 1 469

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216594.6.peg.4665
RefSeq: YP_001852601.1
UniProt: B2HT25

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MANGGGQASD LLVIFGITGD LARKMTFRAL YRLERRGLLD CPIVGVASDD MSVEQLVDRA REVIQGSSEP FDEAVFERFA SRWSYLPGDV TDPALYTALA KQIGHDRHGL YYLEMPPALF APIVENLAKA DLLRGARVAV EKPFGHDLDS ARELNSRLRA VLNEDQILRV DHFLGKQPVV ELQYLRFANR GLAELWDRKS VSEIHVTMAE NFGVEDRGKF YDAVGALRDV VQNHLLQVLA LVAMEPPVGP GADDLNDKKA EVFRAMPALQ TAHYVRGQYQ GYGDVPGVAK DSQTETYVAL RTEIDNWRWA GVPVFLRAGK ALPERVTEVR MFLHHVPGLA FLPNRRPAEP NQIVLRIDPD PGMRLQISAQ TGDSWQGVHL DSSFAEDLGE PIRPYERLLY AGLIGDRQLF AREDAIEQTW RIVQPVLDDP GEIHPYDRGS WGPEAAQTLL RGHRNWQQPW LPRSAEARQ
NT Sequence
TTGGCCAACG GCGGAGGTCA GGCCTCAGAT CTATTGGTGA TCTTTGGAAT TACCGGCGAT CTGGCTCGCA AGATGACTTT TCGCGCGTTG TACAGGCTGG AACGCAGGGG ACTGCTGGAT TGCCCGATCG TGGGCGTCGC CAGTGACGAC ATGTCGGTAG AGCAGTTGGT CGATCGGGCG CGGGAGGTCA TTCAAGGGTC CAGCGAGCCG TTCGACGAAG CGGTATTCGA GCGATTCGCC AGTCGGTGGT CCTATCTGCC CGGTGACGTT ACCGACCCCG CCCTGTACAC GGCGCTGGCC AAGCAGATCG GTCATGACCG CCACGGCTTG TACTACCTGG AGATGCCGCC CGCGCTGTTC GCCCCAATCG TCGAGAATCT TGCCAAGGCA GACTTGCTGC GCGGCGCGCG CGTTGCGGTG GAGAAGCCAT TTGGCCATGA CCTGGACTCC GCGCGGGAAC TCAACTCCCG GCTACGTGCC GTGCTCAACG AAGATCAGAT CCTGCGCGTC GATCACTTCT TGGGCAAGCA ACCCGTCGTG GAGTTGCAGT ATTTGCGCTT CGCCAATCGC GGCCTGGCCG AATTGTGGGA TCGCAAGAGC GTTTCCGAGA TTCACGTCAC CATGGCCGAG AACTTCGGCG TCGAGGACCG TGGCAAGTTC TATGACGCGG TGGGCGCCCT GCGAGACGTC GTGCAAAATC ACCTGCTGCA GGTGCTGGCG CTGGTGGCGA TGGAGCCTCC GGTGGGTCCG GGTGCCGACG ACCTCAACGA CAAGAAGGCC GAGGTGTTCC GTGCCATGCC GGCGCTGCAG ACGGCGCACT ACGTGCGGGG CCAGTACCAG GGTTACGGCG ATGTCCCGGG GGTGGCCAAG GACTCGCAGA CCGAGACCTA TGTCGCACTC AGGACCGAGA TCGACAACTG GCGCTGGGCC GGGGTGCCGG TCTTCTTGCG GGCCGGAAAA GCTCTGCCAG AGCGGGTGAC TGAGGTGCGG ATGTTCCTGC ACCATGTTCC CGGGTTGGCT TTCCTGCCCA ACCGCAGGCC GGCCGAGCCG AACCAGATTG TGCTGCGCAT CGACCCAGAT CCGGGCATGC GCCTGCAGAT ATCCGCGCAA ACCGGCGACT CGTGGCAGGG CGTTCACCTG GACTCCTCGT TCGCCGAAGA CCTCGGTGAA CCGATCCGGC CCTATGAACG ACTGCTGTAT GCCGGATTGA TCGGCGATCG CCAGCTTTTC GCTCGCGAGG ACGCTATCGA GCAGACGTGG CGGATCGTGC AACCGGTTCT TGACGACCCG GGGGAAATCC ACCCGTACGA TCGCGGCTCC TGGGGGCCCG AGGCAGCACA GACGCTGCTG CGTGGCCACC GCAATTGGCA ACAGCCGTGG CTACCCCGCA GTGCCGAGGC ACGACAG
Details for MymaA.00897.a.A1.GE30084
HARVESTED ON: 8/10/2010
SEQUENCED ON: 8/16/2010
EXPECTED MW: 54kDa
OBSERVED MW: 57kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 98
PERCENT COVERAGE: 83
Validated AA Sequence
MLANGGGQAS DLLVIFGITG DLARKMTFRA LYRLERRGLL DCPIVGVASD DMSVEQLVDR AREVIQGSSE PFDEAVFERF ASRWSYLPGD VTDPALYTAL AKQIGHDRHG LYYLEMPPAL FAPIVENLAK ADLLRGARVA VEXPFGHDLD SARELNSRLR AVLNEDXILR VDHFLGKQPV VELQYLRFAN RGLAELWDXK SVSEIHVTMA ENFGVEDRGK FYDAVGALRD VVQNHLLQVL ALVAMEXPVG PGADDXNDKK AEVFRAMPAL QTAHYVRGQY QGYGDVPGVA KDSQTETYVA LRTEIDNWRW AGVPVFLRAG KALPERVTEV RMFLHHVPGL AFLPNRRPAE PNQIVLRIDP DPGMRLQISA QTGDSWQGVH LDSSFAEDLG EPIRPYERLL YAGLIGDR
Validated NT Sequence
ggcgatcgcc gatcaatccg gcatacagca gtcgttcata gggccggatc ggttcaccga ggtcttcggc gaacgaggag tccaggtgaa cgccctgcca cgagtcgccg gtttgcgcgg atatctgcag gcgcatgccc ggatctgggt cgatgcgcag cacaatctgg ttcggctcgg ccggcctgcg gttgggcagg aaagccaacc cgggaacatg gtgcaggaac atccgcacct cagtcacccg ctctggcaga gcttttccgg cccgcaagaa gaccggcacc ccggcccagc gccagttgtc gatctcggtc ctgagtgcga cataggtctc ggtctgcgag tccttggcca cccccgggac atcgccgtaa ccctggtact ggccccgcac gtagtgcgcc gtctgcagcg ccggcatggc acggaacacc tcggccttct tgtcgttgan gtcgtcggca cccggaccca ccggangctc catcgccacc agcgccagca cctgcagcag gtgattttgc acgacgtctc gcagggcgcc caccgcgtca tagaacttgc cacggtcctc gacgccgaag ttctcggcca tggtgacgtg aatctcggaa acgctcttgc natcccacaa ttcggccagg ccgcgattgg cgaagcgcaa atactgcaac tccacgacgg gttgcttgcc caagaagtga tcgacgcgca ggatntgatc ttcgttgagc acggcacgta gccgggagtt gagttcccgc gcggagtcca ggtcatggcc aaatggnttc tccaccgcaa cgcgcgcgcc gcgcagcaag tctgccttgg caagattctc gacgattggg gcgaacagcg cgggcggcat ctccaggtag tacaagccgt ggcggtcatg accgatctgc ttggccagcg ccgtgtacag ggcggggtcg gtaacgtcac cgggcagata ggaccaccga ctggcgaatc gctcgaatac cgcttcgtcg aacggctcgc tggacccttg aatgacctcc cgcgcccgat cgaccaactg ctctaccgac atgtcgtcac tggcgacgcc cacgatcggg caatccagca gtcccctgcg ttccagcctg tacaacgcgc gaaaagtcat cttgcgagcc agatcgccgg taattccaaa gatcaccaat agatctgagg cctgacctcc gccgttggcc aacatcgaac caggaccctg ggtctgagct tccagggt
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMLANGGGQA SDLLVIFGIT GDLARKMTFR ALYRLERRGL LDCPIVGVAS DDMSVEQLVD RAREVIQGSS EPFDEAVFER FASRWSYLPG DVTDPALYTA LAKQIGHDRH GLYYLEMPPA LFAPIVENLA KADLLRGARV AVEKPFGHDL DSARELNSRL RAVLNEDQIL RVDHFLGKQP VVELQYLRFA NRGLAELWDR KSVSEIHVTM AENFGVEDRG KFYDAVGALR DVVQNHLLQV LALVAMEPPV GPGADDLNDK KAEVFRAMPA LQTAHYVRGQ YQGYGDVPGV AKDSQTETYV ALRTEIDNWR WAGVPVFLRA GKALPERVTE VRMFLHHVPG LAFLPNRRPA EPNQIVLRID PDPGMRLQIS AQTGDSWQGV HLDSSFAEDL GEPIRPYERL LYAGLIGDRQ LFAREDAIEQ TWRIVQPVLD DPGEIHPYDR GSWGPEAAQT LLRGHRNWQQ PWLPRSAEAR
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gttggccaac ggcggaggtc aggcctcaga tctattggtg atctttggaa ttaccggcga tctggctcgc aagatgactt ttcgcgcgtt gtacaggctg gaacgcaggg gactgctgga ttgcccgatc gtgggcgtcg ccagtgacga catgtcggta gagcagttgg tcgatcgggc gcgggaggtc attcaagggt ccagcgagcc gttcgacgaa gcggtattcg agcgattcgc cagtcggtgg tcctatctgc ccggtgacgt taccgacccc gccctgtaca cggcgctggc caagcagatc ggtcatgacc gccacggctt gtactacctg gagatgccgc ccgcgctgtt cgccccaatc gtcgagaatc ttgccaaggc agacttgctg cgcggcgcgc gcgttgcggt ggagaagcca tttggccatg acctggactc cgcgcgggaa ctcaactccc ggctacgtgc cgtgctcaac gaagatcaga tcctgcgcgt cgatcacttc ttgggcaagc aacccgtcgt ggagttgcag tatttgcgct tcgccaatcg cggcctggcc gaattgtggg atcgcaagag cgtttccgag attcacgtca ccatggccga gaacttcggc gtcgaggacc gtggcaagtt ctatgacgcg gtgggcgccc tgcgagacgt cgtgcaaaat cacctgctgc aggtgctggc gctggtggcg atggagcctc cggtgggtcc gggtgccgac gacctcaacg acaagaaggc cgaggtgttc cgtgccatgc cggcgctgca gacggcgcac tacgtgcggg gccagtacca gggttacggc gatgtcccgg gggtggccaa ggactcgcag accgagacct atgtcgcact caggaccgag atcgacaact ggcgctgggc cggggtgccg gtcttcttgc gggccggaaa agctctgcca gagcgggtga ctgaggtgcg gatgttcctg caccatgttc ccgggttggc tttcctgccc aaccgcaggc cggccgagcc gaaccagatt gtgctgcgca tcgacccaga tccgggcatg cgcctgcaga tatccgcgca aaccggcgac tcgtggcagg gcgttcacct ggactcctcg ttcgccgaag acctcggtga accgatccgg ccctatgaac gactgctgta tgccggattg atcggcgatc gccagctttt cgctcgcgag gacgctatcg agcagacgtg gcggatcgtg caaccggttc ttgacgaccc gggggaaatc cacccgtacg atcgcggctc ctgggggccc gaggcagcac agacgctgct gcgtggccac cgcaattggc aacagccgtg gctaccccgc agtgccgagg cacgaaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa
Details for MymaA.00897.a.A1.PS00980
PURIFICATION DATe: 2/21/2011
CONCENTRATION: 17.43mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 5
VIAL VOLUME: 50µl
PERCENT IDENTITY: 98
PERCENT COVERAGE: 83
Protocol Notes
notes unavailable
Validated AA Sequence
MLANGGGQAS DLLVIFGITG DLARKMTFRA LYRLERRGLL DCPIVGVASD DMSVEQLVDR AREVIQGSSE PFDEAVFERF ASRWSYLPGD VTDPALYTAL AKQIGHDRHG LYYLEMPPAL FAPIVENLAK ADLLRGARVA VEXPFGHDLD SARELNSRLR AVLNEDXILR VDHFLGKQPV VELQYLRFAN RGLAELWDXK SVSEIHVTMA ENFGVEDRGK FYDAVGALRD VVQNHLLQVL ALVAMEXPVG PGADDXNDKK AEVFRAMPAL QTAHYVRGQY QGYGDVPGVA KDSQTETYVA LRTEIDNWRW AGVPVFLRAG KALPERVTEV RMFLHHVPGL AFLPNRRPAE PNQIVLRIDP DPGMRLQISA QTGDSWQGVH LDSSFAEDLG EPIRPYERLL YAGLIGDR
Validated NT Sequence
ggcgatcgcc gatcaatccg gcatacagca gtcgttcata gggccggatc ggttcaccga ggtcttcggc gaacgaggag tccaggtgaa cgccctgcca cgagtcgccg gtttgcgcgg atatctgcag gcgcatgccc ggatctgggt cgatgcgcag cacaatctgg ttcggctcgg ccggcctgcg gttgggcagg aaagccaacc cgggaacatg gtgcaggaac atccgcacct cagtcacccg ctctggcaga gcttttccgg cccgcaagaa gaccggcacc ccggcccagc gccagttgtc gatctcggtc ctgagtgcga cataggtctc ggtctgcgag tccttggcca cccccgggac atcgccgtaa ccctggtact ggccccgcac gtagtgcgcc gtctgcagcg ccggcatggc acggaacacc tcggccttct tgtcgttgan gtcgtcggca cccggaccca ccggangctc catcgccacc agcgccagca cctgcagcag gtgattttgc acgacgtctc gcagggcgcc caccgcgtca tagaacttgc cacggtcctc gacgccgaag ttctcggcca tggtgacgtg aatctcggaa acgctcttgc natcccacaa ttcggccagg ccgcgattgg cgaagcgcaa atactgcaac tccacgacgg gttgcttgcc caagaagtga tcgacgcgca ggatntgatc ttcgttgagc acggcacgta gccgggagtt gagttcccgc gcggagtcca ggtcatggcc aaatggnttc tccaccgcaa cgcgcgcgcc gcgcagcaag tctgccttgg caagattctc gacgattggg gcgaacagcg cgggcggcat ctccaggtag tacaagccgt ggcggtcatg accgatctgc ttggccagcg ccgtgtacag ggcggggtcg gtaacgtcac cgggcagata ggaccaccga ctggcgaatc gctcgaatac cgcttcgtcg aacggctcgc tggacccttg aatgacctcc cgcgcccgat cgaccaactg ctctaccgac atgtcgtcac tggcgacgcc cacgatcggg caatccagca gtcccctgcg ttccagcctg tacaacgcgc gaaaagtcat cttgcgagcc agatcgccgg taattccaaa gatcaccaat agatctgagg cctgacctcc gccgttggcc aacatcgaac caggaccctg ggtctgagct tccagggt
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMLANGGGQA SDLLVIFGIT GDLARKMTFR ALYRLERRGL LDCPIVGVAS DDMSVEQLVD RAREVIQGSS EPFDEAVFER FASRWSYLPG DVTDPALYTA LAKQIGHDRH GLYYLEMPPA LFAPIVENLA KADLLRGARV AVEKPFGHDL DSARELNSRL RAVLNEDQIL RVDHFLGKQP VVELQYLRFA NRGLAELWDR KSVSEIHVTM AENFGVEDRG KFYDAVGALR DVVQNHLLQV LALVAMEPPV GPGADDLNDK KAEVFRAMPA LQTAHYVRGQ YQGYGDVPGV AKDSQTETYV ALRTEIDNWR WAGVPVFLRA GKALPERVTE VRMFLHHVPG LAFLPNRRPA EPNQIVLRID PDPGMRLQIS AQTGDSWQGV HLDSSFAEDL GEPIRPYERL LYAGLIGDRQ LFAREDAIEQ TWRIVQPVLD DPGEIHPYDR GSWGPEAAQT LLRGHRNWQQ PWLPRSAEAR
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTTGGCCAAC GGCGGAGGTC AGGCCTCAGA TCTATTGGTG ATCTTTGGAA TTACCGGCGA TCTGGCTCGC AAGATGACTT TTCGCGCGTT GTACAGGCTG GAACGCAGGG GACTGCTGGA TTGCCCGATC GTGGGCGTCG CCAGTGACGA CATGTCGGTA GAGCAGTTGG TCGATCGGGC GCGGGAGGTC ATTCAAGGGT CCAGCGAGCC GTTCGACGAA GCGGTATTCG AGCGATTCGC CAGTCGGTGG TCCTATCTGC CCGGTGACGT TACCGACCCC GCCCTGTACA CGGCGCTGGC CAAGCAGATC GGTCATGACC GCCACGGCTT GTACTACCTG GAGATGCCGC CCGCGCTGTT CGCCCCAATC GTCGAGAATC TTGCCAAGGC AGACTTGCTG CGCGGCGCGC GCGTTGCGGT GGAGAAGCCA TTTGGCCATG ACCTGGACTC CGCGCGGGAA CTCAACTCCC GGCTACGTGC CGTGCTCAAC GAAGATCAGA TCCTGCGCGT CGATCACTTC TTGGGCAAGC AACCCGTCGT GGAGTTGCAG TATTTGCGCT TCGCCAATCG CGGCCTGGCC GAATTGTGGG ATCGCAAGAG CGTTTCCGAG ATTCACGTCA CCATGGCCGA GAACTTCGGC GTCGAGGACC GTGGCAAGTT CTATGACGCG GTGGGCGCCC TGCGAGACGT CGTGCAAAAT CACCTGCTGC AGGTGCTGGC GCTGGTGGCG ATGGAGCCTC CGGTGGGTCC GGGTGCCGAC GACCTCAACG ACAAGAAGGC CGAGGTGTTC CGTGCCATGC CGGCGCTGCA GACGGCGCAC TACGTGCGGG GCCAGTACCA GGGTTACGGC GATGTCCCGG GGGTGGCCAA GGACTCGCAG ACCGAGACCT ATGTCGCACT CAGGACCGAG ATCGACAACT GGCGCTGGGC CGGGGTGCCG GTCTTCTTGC GGGCCGGAAA AGCTCTGCCA GAGCGGGTGA CTGAGGTGCG GATGTTCCTG CACCATGTTC CCGGGTTGGC TTTCCTGCCC AACCGCAGGC CGGCCGAGCC GAACCAGATT GTGCTGCGCA TCGACCCAGA TCCGGGCATG CGCCTGCAGA TATCCGCGCA AACCGGCGAC TCGTGGCAGG GCGTTCACCT GGACTCCTCG TTCGCCGAAG ACCTCGGTGA ACCGATCCGG CCCTATGAAC GACTGCTGTA TGCCGGATTG ATCGGCGATC GCCAGCTTTT CGCTCGCGAG GACGCTATCG AGCAGACGTG GCGGATCGTG CAACCGGTTC TTGACGACCC GGGGGAAATC CACCCGTACG ATCGCGGCTC CTGGGGGCCC GAGGCAGCAC AGACGCTGCT GCGTGGCCAC CGCAATTGGC AACAGCCGTG GCTACCCCGC AGTGCCGAGG CACGAAAACA GCACGAACAA GTTCTGCAGC CAAGCTTCTC GAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAA