MyulA.00897.a

Glucose-6-phosphate 1-dehydrogenase

CENTER ID: MyulA.00897.a
ORGANISM: Mycobacterium ulcerans Agy99
ASSOCIATED DISEASE: Buruli, Bairnsdale ulcer
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyulA.00897.a.A1.GE29747 Full length( MyulA.00897.a ) 1 338
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|362242.7.peg.260
RefSeq: YP_904377.1
UniProt: A0PKN7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLRCARVAVE KPFGHDLDSA RELNSRLRAV LNEDQILRVD HFLGKQPVVE LQYLRFANRD LAELWDRKSV SEIHVTMAEN FGVEDRGKFY DAVGALRDVV QNHLLQVLAL VAMEPPVGPG ADDLNDKKAE VFRAMPALQT AHYVRGQYQG YGDVPGVAKD SQTETYVALR TEIDNWRWAG VPVFLRAGKA LPERVTEVRM FLHHVPGLAF LPNRRPAEPN QIVLRIDPDP GMRLQISAQT GDSWQGVHLD SSFAEDLGEP IRPYERLLYA GLIGDRQLFA REDAIEQTWR IVQPVLDDPG EIHPYDRGSW GPEAAQTLLR GHRNWQQPWL PRSAEARQ
NT Sequence
TTGCTGCGCT GCGCGCGCGT TGCGGTGGAG AAGCCATTTG GCCATGACCT GGACTCCGCG CGGGAACTCA ACTCCCGGCT ACGTGCCGTG CTCAACGAAG ATCAGATCCT GCGCGTCGAT CACTTCTTGG GCAAGCAACC CGTCGTGGAG TTGCAGTATT TGCGCTTCGC CAATCGCGAC CTGGCCGAAT TGTGGGATCG CAAGAGCGTT TCCGAGATTC ACGTCACCAT GGCCGAGAAC TTCGGCGTCG AGGACCGTGG CAAGTTCTAT GACGCGGTGG GCGCCCTGCG AGACGTCGTG CAAAATCACC TGCTGCAGGT GCTGGCGCTG GTGGCGATGG AGCCTCCGGT GGGTCCGGGT GCCGACGATC TCAACGACAA GAAGGCCGAG GTGTTCCGTG CCATGCCGGC GCTGCAGACG GCGCACTACG TGCGGGGCCA GTACCAGGGT TACGGCGATG TCCCGGGGGT GGCCAAGGAC TCGCAGACCG AGACCTATGT CGCACTCAGG ACCGAGATCG ACAACTGGCG CTGGGCCGGG GTGCCGGTCT TCTTGCGGGC CGGAAAAGCT CTGCCAGAGC GGGTAACTGA GGTGCGGATG TTCCTGCACC ATGTTCCCGG GTTGGCTTTC CTGCCCAACC GCAGGCCGGC CGAGCCGAAC CAGATTGTGC TGCGCATCGA CCCAGATCCG GGCATGCGCC TGCAGATATC CGCGCAAACC GGCGACTCGT GGCAGGGCGT TCACCTGGAC TCCTCGTTCG CCGAAGACCT CGGTGAACCG ATCCGGCCCT ATGAACGACT GCTGTATGCC GGATTGATCG GCGATCGCCA GCTTTTCGCT CGCGAGGACG CTATCGAGCA GACGTGGCGG ATCGTGCAAC CGGTTCTTGA CGACCCGGGG GAAATTCACC CGTACGATCG CGGCTCCTGG GGGCCCGAGG CAGCACAGAC GCTGCTGCGT GGTCACCGCA ATTGGCAACA GCCGTGGCTA CCCCGCAGTG CCGAGGCACG ACAG
Details for MyulA.00897.a.A1.GE29747
HARVESTED ON: 6/29/2010
SEQUENCED ON: 7/7/2010
EXPECTED MW: 40kDa
OBSERVED MW: 40kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 91
PERCENT COVERAGE: 88
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMLLRCARVA VEKPFGHDLD SARELNSRLR AVLNEDQILR VDHFLGKQPV VELQYLRFAN RDLAELWDRK SVSEIHVTMA ENFGVEDRGK FYDAVGALRD VVQNHLLQVL ALVAMEPPVG PGADDLNDKK AEVFRAMPAL QTAHYVRGQY QGYGDVPGVA KDSQTETYVA LRTEIDNWRW AGVPVFLRAG KALPERVTEV RMFLHHVPGL AFLPNRRPAE PNQIVLRIDP DPGMRLQIXA QXGDSWXGVH LDSSFAEXLG EXIRXYERXL XXGXXXXRQL FXRXXRXRAX VAXXXXXS
Validated NT Sequence
ttttntttan ctttaagaag gagatatacc atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgttgc tgcgctgcgc gcgcgttgcg gtggagaagc catttggcca tgacctggac tccgcgcggg aactcaactc ccggctacgt gccgtgctca acgaagatca gatcctgcgc gtcgatcact tcttgggcaa gcaacccgtc gtggagttgc agtatttgcg cttcgccaat cgcgacctgg ccgaattgtg ggatcgcaag agcgtttccg agattcacgt caccatggcc gagaacttcg gcgtcgagga ccgtggcaag ttctatgacg cggtgggcgc cctgcgagac gtcgtgcaaa atcacctgct gcaggtgctg gcgctggtgg cgatggagcc tccggtgggt ccgggtgccg acgatctcaa cgacaagaag gccgaggtgt tccgtgccat gccggcgctg cagacggcgc actacgtgcg gggccagtac cagggttacg gcgatgtccc gggggtggcc aaggactcgc agaccgagac ctatgtcgca ctcaggaccg agatcgacaa ctggcgctgg gccggggtgc cggtcttctt gcgggccgga aaagctctgc cagagcgggt aactgaggtg cggatgttcc tgcaccatgt tcccgggttg gctttcctgc ccaaccgcag gccggccgag ccgaaccaga ttgtgctgcg catcgaccca gatccgggca tgcgcctgca gatatncgcg caaancggcg actcgtggca nggngtncac ctggactcct cgttcgccga anacctcggt gaancgatcc ggncctatga acgantgctg natgncggan tgatnngcna tcgccagctt ttcnctcgcg angnacgcta ncgagcanac gtggcgnatc nngnnancgn ntcntgacga cnngggggaa nntcnnnncg nnacnatcgc ngcttncngg nggnccnnna ngcannnnnn nnnnnnncng cgtnnnnncn nnnantgnna nnnnnnngnn gnnnnccnnn nannnnnnnn nnnnnnnnnn nnnnannnnn nnnannnnnn nngnnnncnn nnntnnnnnn gnnnncnnnn gntnnna
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMLLRCARVA VEKPFGHDLD SARELNSRLR AVLNEDQILR VDHFLGKQPV VELQYLRFAN RDLAELWDRK SVSEIHVTMA ENFGVEDRGK FYDAVGALRD VVQNHLLQVL ALVAMEPPVG PGADDLNDKK AEVFRAMPAL QTAHYVRGQY QGYGDVPGVA KDSQTETYVA LRTEIDNWRW AGVPVFLRAG KALPERVTEV RMFLHHVPGL AFLPNRRPAE PNQIVLRIDP DPGMRLQISA QTGDSWQGVH LDSSFAEDLG EPIRPYERLL YAGLIGDRQL FAREDAIEQT WRIVQPVLDD PGEIHPYDRG SWGPEAAQTL LRGHRNWQQP WLPRSAEARQ
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gttgctgcgc tgcgcgcgcg ttgcggtgga gaagccattt ggccatgacc tggactccgc gcgggaactc aactcccggc tacgtgccgt gctcaacgaa gatcagatcc tgcgcgtcga tcacttcttg ggcaagcaac ccgtcgtgga gttgcagtat ttgcgcttcg ccaatcgcga cctggccgaa ttgtgggatc gcaagagcgt ttccgagatt cacgtcacca tggccgagaa cttcggcgtc gaggaccgtg gcaagttcta tgacgcggtg ggcgccctgc gagacgtcgt gcaaaatcac ctgctgcagg tgctggcgct ggtggcgatg gagcctccgg tgggtccggg tgccgacgat ctcaacgaca agaaggccga ggtgttccgt gccatgccgg cgctgcagac ggcgcactac gtgcggggcc agtaccaggg ttacggcgat gtcccggggg tggccaagga ctcgcagacc gagacctatg tcgcactcag gaccgagatc gacaactggc gctgggccgg ggtgccggtc ttcttgcggg ccggaaaagc tctgccagag cgggtaactg aggtgcggat gttcctgcac catgttcccg ggttggcttt cctgcccaac cgcaggccgg ccgagccgaa ccagattgtg ctgcgcatcg acccagatcc gggcatgcgc ctgcagatat ccgcgcaaac cggcgactcg tggcagggcg ttcacctgga ctcctcgttc gccgaagacc tcggtgaacc gatccggccc tatgaacgac tgctgtatgc cggattgatc ggcgatcgcc agcttttcgc tcgcgaggac gctatcgagc agacgtggcg gatcgtgcaa ccggttcttg acgacccggg ggaaattcac ccgtacgatc gcggctcctg ggggcccgag gcagcacaga cgctgctgcg tggtcaccgc aattggcaac agccgtggct accccgcagt gccgaggcac gacagaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa