MyavA.00897.a
Glucose-6-phosphate 1-dehydrogenase
| CENTER ID: | MyavA.00897.a |
| ORGANISM: | Mycobacterium avium 104 |
| ASSOCIATED DISEASE: | Johne's disease, paratuberculosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIC |
Ordering Clones & Proteins
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MyavA.00897.a.A1.GE34927 | Full length( MyavA.00897.a ) | 1 | 469 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MyavA.00897.a.A1.PW35542 | Full length( MyavA.00897.a ) | 1 | 469 |
Structures
4LGV
DEPOSITED: 6/28/2013
DETERMINATION: XRay
CLONE: MyavA.00897.a.A1.GE34927
PROTEIN: MyavA.00897.a.A1.PW35542
Publications by SSGCID
Increasing the structural coverage of tuberculosis drug targets.
Abendroth J, Abramov A, Armour B, Barrett LK, Baugh L, Begley DW, Buchko GW, Choi R, Clifton MC, Dieterich SH, Dranow DM, Edwards TE, Fairman JW, Ferrell M, Fox D, Gardberg AS, Hewitt SN, Lorimer D, Lyons-Abbott S, Mundt E, Muruthi MM, Myers J, Myler PJ, Napuli AJ, Phan I, Sekar A, Serbzhinskiy D, Stacy R, Staker BL, Stewart LJ, Taylor BM, Thompkins K, Tran N, Van Voorhis WC, Zhang Y
Tuberculosis (Edinb) - 2014
volume 95, issue 2, pages 142-8
PMID: 25613812; PMCID: PMC4361283
PDB:
3GVC,
3GVG,
3GWC,
3H7F,
3H81,
3HE2,
3HWI,
3HWK,
3HZG,
3ICO,
3KHP,
3LLS,
3MOY,
3MPZ,
3MYB,
3NDN,
3NDO,
3NF4,
3NG3,
3NJD,
3NWO,
3O0M,
3O38,
3OC6,
3OC7,
3OEC,
3OI9,
3OKS,
3OL3,
3OL4,
3OME,
3P0T,
3P2Y,
3P4I,
3P4T,
3P5M,
3P85,
3PE8,
3PGX,
3PK0,
3PPI,
3PXX,
3PZY,
3Q1T,
3Q8N,
3QBP,
3QDF,
3QH4,
3QHA,
3QIV,
3QK8,
3QKA,
3QLJ,
3QMJ,
3QRE,
3QUA,
3QUV,
3QXI,
3QXZ,
3QYR,
3R0O,
3R1I,
3R1J,
3R20,
3R2N,
3R4T,
3R6H,
3R6O,
3R7K,
3R8C,
3R9P,
3R9Q,
3R9R,
3R9S,
3R9T,
3RD5,
3RD7,
3RD8,
3RFQ,
3RIH,
3RR2,
3RR6,
3RRP,
3RRV,
3RSI,
3RV2,
3S55,
3S82,
3SBX,
3SF6,
3SLL,
3SVK,
3SVT,
3SWO,
3SWT,
3SWX,
3SX2,
3T3W,
3T7C,
3TAV,
3TCR,
3TDE,
3TJR,
3TL3,
3TLF,
3TRR,
3TSC,
3TX2,
3TZQ,
3TZU,
3U0A,
3U0B,
3UCX,
3UVE,
4DI1,
4DIE,
4DQ8,
4DXL,
4ED4,
4EGE,
4EGF,
4EMD,
4EO9,
4EYE,
4F3W,
4F47,
4FFC,
4GK6,
4HDT,
4HR3,
4I1Y,
4IJN,
4IV6,
4IZ9,
4J5I,
4KAM,
4LGV,
4O2D,
5EJ2
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| BV-BRC: | fig|243243.7.peg.1225 |
| RefSeq: | YP_880497.1 |
| UniProt: | A0A0H3A0Q9 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MADDDSHPSD LLVIFGITGD LARKMTFRAL YRLERREELE HPIIGVASDD ITLDQLLDRA REAIKATGET FDDAVFDRLA GRLSYLSGDV TDTGLYSELA EKIGGDSRPL YYLEMPPSLF APIVENLAKA DLLERARVAV EKPFGHDLES ARDLNARLRA VLDEDQILRV DHFLGKQPVE ELQYLRFANN ALAKLWDRDS ISEIHITMAE DFGIEDRGKF YDAVGAVRDV VQNHLLQVLA LVAMEPPVGA GADDLNDKKA EVFRAMPSLD PEHCVRGQYR GYTEVPGVAK DSTTETYVAL RTEIDNWRWA GVPIFLRAGK ALPHKVTEVR MFLHHVPGFS FLPNRRPPEP NQIVLRIDPD PGMRLQLSAQ VGDSWHDVHL DSSFAVDLGE PVRPYERLLY AAFNGDRQLF AREDAIEETW RIVQPVLDKP SRIHQYEQGS WGPEAAQALV HGRHAWQQPW LPQSTSTKR
NT Sequence
TTGGCCGACG ATGACAGCCA TCCATCCGAC CTGCTGGTGA TCTTCGGAAT CACCGGGGAC TTGGCGCGCA AGATGACGTT CCGCGCGCTC TACCGGCTGG AGCGCCGCGA GGAGCTCGAG CACCCGATCA TCGGCGTGGC CAGCGACGAC ATCACCCTCG ACCAGCTGCT GGACCGCGCC CGGGAGGCGA TCAAGGCCAC CGGTGAGACG TTCGACGACG CGGTGTTCGA CCGCTTGGCG GGCCGGCTTT CCTACCTGTC GGGCGACGTC ACCGACACCG GGCTCTACAG CGAGCTCGCC GAGAAGATCG GTGGCGATTC GCGTCCGCTG TACTACCTGG AGATGCCGCC GTCGTTGTTC GCGCCGATCG TGGAGAACCT GGCCAAGGCC GACCTACTGG AACGCGCCAG GGTGGCGGTG GAAAAGCCGT TCGGCCACGA CCTGGAATCG GCGCGCGACC TCAACGCCCG GCTGCGCGCG GTGCTCGACG AAGACCAAAT CCTGCGCGTA GACCACTTTT TGGGCAAACA GCCGGTCGAA GAACTGCAAT ACCTGCGGTT CGCCAACAAC GCCCTGGCCA AGCTGTGGGA CCGCGACAGC ATCTCCGAAA TCCACATCAC CATGGCCGAG GACTTCGGCA TCGAGGACCG CGGCAAGTTC TATGACGCGG TGGGGGCGGT GCGCGACGTC GTGCAAAACC ATCTGCTGCA GGTGCTCGCG CTGGTCGCCA TGGAACCCCC GGTCGGGGCC GGCGCCGACG ACCTCAACGA CAAGAAGGCC GAGGTGTTCC GGGCCATGCC GTCGCTGGAC CCGGAGCACT GCGTGCGTGG CCAGTATCGT GGCTACACCG AAGTCCCCGG TGTGGCAAAG GATTCCACCA CCGAGACCTA CGTCGCGTTG AGGACCGAGA TCGACAACTG GCGCTGGGCC GGGGTGCCGA TCTTCCTGCG CGCCGGCAAG GCGTTGCCGC ACAAGGTCAC CGAGGTCCGG ATGTTCCTGC ATCACGTGCC CGGCTTCTCC TTCCTGCCCA ACCGCCGCCC GCCCGAGCCC AACCAGATCG TGCTGCGCAT CGACCCCGAT CCCGGGATGC GCCTGCAGCT GTCCGCTCAG GTCGGCGACT CGTGGCACGA CGTCCACCTG GACTCCTCGT TCGCGGTGGA CCTCGGCGAA CCGGTGCGGC CCTACGAACG GCTGCTCTAC GCCGCGTTCA ACGGCGACCG CCAGCTGTTT GCCCGCGAGG ACGCCATCGA AGAGACCTGG CGGATCGTGC AGCCGGTGCT GGACAAGCCG AGCCGCATCC ACCAATACGA GCAAGGCTCC TGGGGACCCG AGGCCGCGCA GGCGCTGGTG CACGGTCGCC ACGCCTGGCA GCAACCGTGG CTGCCGCAGA GCACGTCCAC GAAACGA
Details for MyavA.00897.a.A1.GE34927
| HARVESTED ON: | 4/17/2012 |
| SEQUENCED ON: | 4/17/2012 |
| EXPECTED MW: | 55kDa |
| OBSERVED MW: | 55kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 99 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMLADDDSHP SDLLVIFGIT GDLARKMTFR ALYRLERREE LEHPIIGVAS DDITLDQLLD RAREAIKATG ETFDDAVFDR LAGRLSYLSG DVTDTGLYSE LAEKIGGDSR PLYYLEMPPS LFAPIVENLA KADLLERARV AVEKPFGHDL ESARDLNARL RAVLDEDQIL RVDHFLGKQP VEELQYLRFA NNALAKLWDR DSISEIHITM AEDFGIEDRG KFYDAVGAVR DVVQNHLLQV LALVAMEPPV GAGADDLNDK KAEVFRAMPS LDPEHCVRGQ YRGYTEVPGV AKDSTTETYV ALRTEIDNWR WAGVPIFLRA GKALPHKVTE VRMFLHHVPG FSFLPNRRPP EPNQIVLRID PDPGMRLQLS AQVGDSWHDV HLDSSFAVDL GEPVRPYERL LYAAFNGDRQ LFAREDAIEE TWRIVQPVLD KPSRIHQYEQ GSWGPEAAQA LVHGRHAWQQ PWLPQSTST
Validated NT Sequence
tcgtggacgt gctctgcggc agccacggtt gctgccaggc gtggcgaccg tgcaccagcg cctgcgcggc ctcgggtccc caggagcctt gctcgtattg gtggatgcgg ctcggcttgt ccagcaccgg ctgcacgatc cgccaggtct cttcgatggc gtcctcgcgg gcaaacagct ggcggtcgcc gttgaacgcg gcgtagagca gccgttcgta gggccgcacc ggttcgccga ggtccaccgc gaacgaggag tccaggtgga cgtcgtgcca cgagtcgccg acctgagcgg acagctgcag gcgcatcccg ggatcggggt cgatgcgcag cacgatctgg ttgggctcgg gcgggcggcg gttgggcagg aaggagaagc cgggcacgtg atgcaggaac atccggacct cggtgacctt gtgcggcaac gccttgccgg cgcgcaggaa gatcggcacc ccggcccagc gccagttgtc gatctcggtc ctcaacgcga cgtaggtctc ggtggtggaa tcctttgcca caccggggac ttcggtgtag ccacgatact ggccacgcac gcagtgctcc gggtccagcg acggcatggc ccggaacacc tcggccttct tgtcgttgag gtcgtcggcg ccggccccga ccgggggttc catggcgacc agcgcgagca cctgcagcag atggttttgc acgacgtcgc gcaccgcccc caccgcgtca tagaacttgc cgcggtcctc gatgccgaag tcctcggcca tggtgatgtg gatttcggag atgctgtcgc ggtcccacag cttggccagg gcgttgttgg cgaaccgcag gtattgcagt tcttcgaccg gctgtttgcc caaaaagtgg tctacgcgca ggatttggtc ttcgtcgagc accgcgcgca gccgggcgtt gaggtcgcgc gccgattcca ggtcgtggcc gaacggcttt tccaccgcca ccctggcgcg ttccagtagg tcggccttgg ccaggttctc cacgatcggc gcgaacaacg acggcggcat ctccaggtag tacagcggac gcgaatcgcc accgatcttc tcggcgagct cgctgtagag cccggtgtcg gtgacgtcgc ccgacaggta ggaaagccgg cccgccaagc ggtcgaacac cgcgtcgtcg aacgtctcac cggtggcctt gatcgcctcc cgggcgcggt ccagcagctg gtcgagggtg atgtcgtcgc tggccacgcc gatgatcggg tgctcgagct cctcgcggcg ctccagccgg tagagcgcgc ggaacgtcat cttgcgcgcc aagtccccgg tgattccgaa gatcaccagc aggtcggatg gatggctgtc atcgtcggcc aacatcgaac caggaccctg ggtctgagct tccagggtac ccatatgatg gtgatggtga tgagccatgg tat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMLADDDSHP SDLLVIFGIT GDLARKMTFR ALYRLERREE LEHPIIGVAS DDITLDQLLD RAREAIKATG ETFDDAVFDR LAGRLSYLSG DVTDTGLYSE LAEKIGGDSR PLYYLEMPPS LFAPIVENLA KADLLERARV AVEKPFGHDL ESARDLNARL RAVLDEDQIL RVDHFLGKQP VEELQYLRFA NNALAKLWDR DSISEIHITM AEDFGIEDRG KFYDAVGAVR DVVQNHLLQV LALVAMEPPV GAGADDLNDK KAEVFRAMPS LDPEHCVRGQ YRGYTEVPGV AKDSTTETYV ALRTEIDNWR WAGVPIFLRA GKALPHKVTE VRMFLHHVPG FSFLPNRRPP EPNQIVLRID PDPGMRLQLS AQVGDSWHDV HLDSSFAVDL GEPVRPYERL LYAAFNGDRQ LFAREDAIEE TWRIVQPVLD KPSRIHQYEQ GSWGPEAAQA LVHGRHAWQQ PWLPQSTSTK R
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gttggccgac gatgacagcc atccatccga cctgctggtg atcttcggaa tcaccgggga cttggcgcgc aagatgacgt tccgcgcgct ctaccggctg gagcgccgcg aggagctcga gcacccgatc atcggcgtgg ccagcgacga catcaccctc gaccagctgc tggaccgcgc ccgggaggcg atcaaggcca ccggtgagac gttcgacgac gcggtgttcg accgcttggc gggccggctt tcctacctgt cgggcgacgt caccgacacc gggctctaca gcgagctcgc cgagaagatc ggtggcgatt cgcgtccgct gtactacctg gagatgccgc cgtcgttgtt cgcgccgatc gtggagaacc tggccaaggc cgacctactg gaacgcgcca gggtggcggt ggaaaagccg ttcggccacg acctggaatc ggcgcgcgac ctcaacgccc ggctgcgcgc ggtgctcgac gaagaccaaa tcctgcgcgt agaccacttt ttgggcaaac agccggtcga agaactgcaa tacctgcggt tcgccaacaa cgccctggcc aagctgtggg accgcgacag catctccgaa atccacatca ccatggccga ggacttcggc atcgaggacc gcggcaagtt ctatgacgcg gtgggggcgg tgcgcgacgt cgtgcaaaac catctgctgc aggtgctcgc gctggtcgcc atggaacccc cggtcggggc cggcgccgac gacctcaacg acaagaaggc cgaggtgttc cgggccatgc cgtcgctgga cccggagcac tgcgtgcgtg gccagtatcg tggctacacc gaagtccccg gtgtggcaaa ggattccacc accgagacct acgtcgcgtt gaggaccgag atcgacaact ggcgctgggc cggggtgccg atcttcctgc gcgccggcaa ggcgttgccg cacaaggtca ccgaggtccg gatgttcctg catcacgtgc ccggcttctc cttcctgccc aaccgccgcc cgcccgagcc caaccagatc gtgctgcgca tcgaccccga tcccgggatg cgcctgcagc tgtccgctca ggtcggcgac tcgtggcacg acgtccacct ggactcctcg ttcgcggtgg acctcggcga accggtgcgg ccctacgaac ggctgctcta cgccgcgttc aacggcgacc gccagctgtt tgcccgcgag gacgccatcg aagagacctg gcggatcgtg cagccggtgc tggacaagcc gagccgcatc caccaatacg agcaaggctc ctggggaccc gaggccgcgc aggcgctggt gcacggtcgc cacgcctggc agcaaccgtg gctgccgcag agcacgtcca cgaaacgaaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa
Details for MyavA.00897.a.A1.PW35542
| PURIFICATION DATe: | 8/20/2012 |
| CONCENTRATION: | 21.57mg/ml |
| OBSERVED MW: | 56kDa |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 13 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 99 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMLADDDSHP SDLLVIFGIT GDLARKMTFR ALYRLERREE LEHPIIGVAS DDITLDQLLD RAREAIKATG ETFDDAVFDR LAGRLSYLSG DVTDTGLYSE LAEKIGGDSR PLYYLEMPPS LFAPIVENLA KADLLERARV AVEKPFGHDL ESARDLNARL RAVLDEDQIL RVDHFLGKQP VEELQYLRFA NNALAKLWDR DSISEIHITM AEDFGIEDRG KFYDAVGAVR DVVQNHLLQV LALVAMEPPV GAGADDLNDK KAEVFRAMPS LDPEHCVRGQ YRGYTEVPGV AKDSTTETYV ALRTEIDNWR WAGVPIFLRA GKALPHKVTE VRMFLHHVPG FSFLPNRRPP EPNQIVLRID PDPGMRLQLS AQVGDSWHDV HLDSSFAVDL GEPVRPYERL LYAAFNGDRQ LFAREDAIEE TWRIVQPVLD KPSRIHQYEQ GSWGPEAAQA LVHGRHAWQQ PWLPQSTST
Validated NT Sequence
tcgtggacgt gctctgcggc agccacggtt gctgccaggc gtggcgaccg tgcaccagcg cctgcgcggc ctcgggtccc caggagcctt gctcgtattg gtggatgcgg ctcggcttgt ccagcaccgg ctgcacgatc cgccaggtct cttcgatggc gtcctcgcgg gcaaacagct ggcggtcgcc gttgaacgcg gcgtagagca gccgttcgta gggccgcacc ggttcgccga ggtccaccgc gaacgaggag tccaggtgga cgtcgtgcca cgagtcgccg acctgagcgg acagctgcag gcgcatcccg ggatcggggt cgatgcgcag cacgatctgg ttgggctcgg gcgggcggcg gttgggcagg aaggagaagc cgggcacgtg atgcaggaac atccggacct cggtgacctt gtgcggcaac gccttgccgg cgcgcaggaa gatcggcacc ccggcccagc gccagttgtc gatctcggtc ctcaacgcga cgtaggtctc ggtggtggaa tcctttgcca caccggggac ttcggtgtag ccacgatact ggccacgcac gcagtgctcc gggtccagcg acggcatggc ccggaacacc tcggccttct tgtcgttgag gtcgtcggcg ccggccccga ccgggggttc catggcgacc agcgcgagca cctgcagcag atggttttgc acgacgtcgc gcaccgcccc caccgcgtca tagaacttgc cgcggtcctc gatgccgaag tcctcggcca tggtgatgtg gatttcggag atgctgtcgc ggtcccacag cttggccagg gcgttgttgg cgaaccgcag gtattgcagt tcttcgaccg gctgtttgcc caaaaagtgg tctacgcgca ggatttggtc ttcgtcgagc accgcgcgca gccgggcgtt gaggtcgcgc gccgattcca ggtcgtggcc gaacggcttt tccaccgcca ccctggcgcg ttccagtagg tcggccttgg ccaggttctc cacgatcggc gcgaacaacg acggcggcat ctccaggtag tacagcggac gcgaatcgcc accgatcttc tcggcgagct cgctgtagag cccggtgtcg gtgacgtcgc ccgacaggta ggaaagccgg cccgccaagc ggtcgaacac cgcgtcgtcg aacgtctcac cggtggcctt gatcgcctcc cgggcgcggt ccagcagctg gtcgagggtg atgtcgtcgc tggccacgcc gatgatcggg tgctcgagct cctcgcggcg ctccagccgg tagagcgcgc ggaacgtcat cttgcgcgcc aagtccccgg tgattccgaa gatcaccagc aggtcggatg gatggctgtc atcgtcggcc aacatcgaac caggaccctg ggtctgagct tccagggtac ccatatgatg gtgatggtga tgagccatgg tat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMLADDDSHP SDLLVIFGIT GDLARKMTFR ALYRLERREE LEHPIIGVAS DDITLDQLLD RAREAIKATG ETFDDAVFDR LAGRLSYLSG DVTDTGLYSE LAEKIGGDSR PLYYLEMPPS LFAPIVENLA KADLLERARV AVEKPFGHDL ESARDLNARL RAVLDEDQIL RVDHFLGKQP VEELQYLRFA NNALAKLWDR DSISEIHITM AEDFGIEDRG KFYDAVGAVR DVVQNHLLQV LALVAMEPPV GAGADDLNDK KAEVFRAMPS LDPEHCVRGQ YRGYTEVPGV AKDSTTETYV ALRTEIDNWR WAGVPIFLRA GKALPHKVTE VRMFLHHVPG FSFLPNRRPP EPNQIVLRID PDPGMRLQLS AQVGDSWHDV HLDSSFAVDL GEPVRPYERL LYAAFNGDRQ LFAREDAIEE TWRIVQPVLD KPSRIHQYEQ GSWGPEAAQA LVHGRHAWQQ PWLPQSTSTK R
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTTGGCCGAC GATGACAGCC ATCCATCCGA CCTGCTGGTG ATCTTCGGAA TCACCGGGGA CTTGGCGCGC AAGATGACGT TCCGCGCGCT CTACCGGCTG GAGCGCCGCG AGGAGCTCGA GCACCCGATC ATCGGCGTGG CCAGCGACGA CATCACCCTC GACCAGCTGC TGGACCGCGC CCGGGAGGCG ATCAAGGCCA CCGGTGAGAC GTTCGACGAC GCGGTGTTCG ACCGCTTGGC GGGCCGGCTT TCCTACCTGT CGGGCGACGT CACCGACACC GGGCTCTACA GCGAGCTCGC CGAGAAGATC GGTGGCGATT CGCGTCCGCT GTACTACCTG GAGATGCCGC CGTCGTTGTT CGCGCCGATC GTGGAGAACC TGGCCAAGGC CGACCTACTG GAACGCGCCA GGGTGGCGGT GGAAAAGCCG TTCGGCCACG ACCTGGAATC GGCGCGCGAC CTCAACGCCC GGCTGCGCGC GGTGCTCGAC GAAGACCAAA TCCTGCGCGT AGACCACTTT TTGGGCAAAC AGCCGGTCGA AGAACTGCAA TACCTGCGGT TCGCCAACAA CGCCCTGGCC AAGCTGTGGG ACCGCGACAG CATCTCCGAA ATCCACATCA CCATGGCCGA GGACTTCGGC ATCGAGGACC GCGGCAAGTT CTATGACGCG GTGGGGGCGG TGCGCGACGT CGTGCAAAAC CATCTGCTGC AGGTGCTCGC GCTGGTCGCC ATGGAACCCC CGGTCGGGGC CGGCGCCGAC GACCTCAACG ACAAGAAGGC CGAGGTGTTC CGGGCCATGC CGTCGCTGGA CCCGGAGCAC TGCGTGCGTG GCCAGTATCG TGGCTACACC GAAGTCCCCG GTGTGGCAAA GGATTCCACC ACCGAGACCT ACGTCGCGTT GAGGACCGAG ATCGACAACT GGCGCTGGGC CGGGGTGCCG ATCTTCCTGC GCGCCGGCAA GGCGTTGCCG CACAAGGTCA CCGAGGTCCG GATGTTCCTG CATCACGTGC CCGGCTTCTC CTTCCTGCCC AACCGCCGCC CGCCCGAGCC CAACCAGATC GTGCTGCGCA TCGACCCCGA TCCCGGGATG CGCCTGCAGC TGTCCGCTCA GGTCGGCGAC TCGTGGCACG ACGTCCACCT GGACTCCTCG TTCGCGGTGG ACCTCGGCGA ACCGGTGCGG CCCTACGAAC GGCTGCTCTA CGCCGCGTTC AACGGCGACC GCCAGCTGTT TGCCCGCGAG GACGCCATCG AAGAGACCTG GCGGATCGTG CAGCCGGTGC TGGACAAGCC GAGCCGCATC CACCAATACG AGCAAGGCTC CTGGGGACCC GAGGCCGCGC AGGCGCTGGT GCACGGTCGC CACGCCTGGC AGCAACCGTG GCTGCCGCAG AGCACGTCCA CGAAACGAAA ACAGCACGAA CAAGTTCTGC AGCCAAGCTT CTCGAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA