MytuD.00693.a

NAD-dependent deacetylase

CENTER ID: MytuD.00693.a
ORGANISM: Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE: Tuberculosis
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MytuD.00693.a.B1.GE27589 Full length( MytuD.00693.a ) 1 237
MytuD.00693.a.B1.GE27168 Full length( MytuD.00693.a ) 1 237
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|83332.12.peg.1292
RefSeq: NP_215667.1
TubercuList: Rv1151c
MTB Network Portal: Rv1151c
UniProt: P9WGG3

Orthologues for Rv1151c

Species % Identity % Coverage Clones Proteins Structures
marinum 88 100 1 1 0
ulcerans 87 100 0 0 0
paratuberculosis 86 100 0 0 0
leprae 85 100 0 0 0
avium 85 98 1 0 0
smegmatis 69 100 1 0 0
abscessus 52 97 1 0 0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRVAVLSGAG ISAESGVPTF RDDKNGLWAR FDPYELSSTQ GWLRNPERVW GWYLWRHYLV ANVEPNDGHR AIAAWQDHAE VSVITQNVDD LHERAGSGAV HHLHGSLFEF RCARCGVPYT DALPEMPEPA IEVEPPVCDC GGLIRPDIVW FGEPLPEEPW RSAVEATGSA DVMVVVGTSA IVYPAAGLPD LALARGTAVI EVNPEPTPLS GSATISIRES ASQALPGLLE RLPALLK
NT Sequence
ATGCGAGTGG CGGTGCTCAG CGGCGCGGGG ATCTCCGCGG AAAGCGGTGT ACCGACGTTC CGCGATGACA AGAACGGATT GTGGGCCCGA TTCGACCCTT ACGAGCTGTC CAGCACGCAA GGCTGGCTGC GCAACCCCGA GCGGGTCTGG GGATGGTACC TATGGCGCCA TTATCTGGTG GCCAACGTCG AACCCAACGA CGGGCATCGC GCGATCGCCG CCTGGCAGGA CCATGCTGAG GTCAGCGTCA TCACCCAGAA TGTCGACGAT CTGCACGAGC GCGCCGGCAG CGGTGCGGTG CATCACCTGC ACGGCAGCCT TTTCGAATTT CGTTGTGCAC GTTGTGGTGT GCCCTACACC GACGCCCTTC CGGAGATGCC CGAGCCTGCG ATCGAAGTGG AGCCGCCGGT CTGCGACTGC GGCGGTCTGA TCCGGCCCGA CATCGTATGG TTCGGTGAGC CGCTGCCAGA GGAGCCGTGG CGGAGCGCGG TCGAGGCGAC AGGGAGCGCC GACGTGATGG TCGTGGTGGG GACCTCGGCG ATCGTCTACC CGGCGGCCGG TTTACCCGAC CTGGCGCTGG CGCGCGGCAC TGCCGTGATC GAAGTCAATC CCGAGCCCAC GCCGTTGTCC GGCAGCGCGA CGATCAGCAT CCGCGAGTCG GCCAGCCAAG CGTTGCCGGG GCTGTTGGAG CGCCTGCCCG CCCTGCTGAA A
Details for MytuD.00693.a.B1.GE27589
HARVESTED ON: 8/17/2009
SEQUENCED ON: 10/20/2009
EXPECTED MW: 26kDa
OBSERVED MW: 27kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMR VAVLSGAGIS AESGVPTFRD DKNGLWARFD PYELSSTQGW LRNPERVWGW YLWRHYLVAN VEPNDGHRAI AAWQDHAEVS VITQNVDDLH ERAGSGAVHH LHGSLFEFRC ARCGVPYTDA LPEMPEPAIE VEPPVCDCGG LIRPDIVWFG EPLPEEPWRS AVEATGSADV MVVVGTSAIV YPAAGLPDLA LARGTAVIEV NPEPTPLSGS ATISIRESAS QALPGLLERL PALLK
Validated NT Sequence
tcttactcac ttatttcagc agggcgggca ggcgctccaa cagccccggc aacgcttggc tggccgactc gcggatgctg atcgtcgcgc tgccggacaa cggcgtgggc tcgggattga cttcgatcac ggcagtgccg cgcgccagcg ccaggtcggg taaaccggcc gccgggtaga cgatcgccga ggtccccacc acgaccatca cgtcggcgct ccctgtcgcc tcgaccgcgc tccgccacgg ctcctctggc agcggctcac cgaaccatac gatgtcgggc cggatcagac cgccgcagtc gcagaccggc ggctccactt cgatcgcagg ctcgggcatc tccggaaggg cgtcggtgta gggcacacca caacgtgcac aacgaaattc gaaaaggctg ccgtgcaggt gatgcaccgc accgctgccg gcgcgctcgt gcagatcgtc gacattctgg gtgatgacgc tgacctcagc atggtcctgc caggcggcga tcgcgcgatg cccgtcgttg ggttcgacgt tggccaccag ataatggcgc cataggtacc atccccagac ccgctcgggg ttgcgcagcc agccttgcgt gctggacagc tcgtaagggt cgaatcgggc ccacaatccg ttcttgtcat cgcggaacgt cggtacaccg ctttccgcgg agatccccgc gccgctgagc accgccactc gcatatggtg gtggtggtgg tgagccat
Expected Protein Sequence
MAHHHHHHMR VAVLSGAGIS AESGVPTFRD DKNGLWARFD PYELSSTQGW LRNPERVWGW YLWRHYLVAN VEPNDGHRAI AAWQDHAEVS VITQNVDDLH ERAGSGAVHH LHGSLFEFRC ARCGVPYTDA LPEMPEPAIE VEPPVCDCGG LIRPDIVWFG EPLPEEPWRS AVEATGSADV MVVVGTSAIV YPAAGLPDLA LARGTAVIEV NPEPTPLSGS ATISIRESAS QALPGLLERL PALLK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcgag tggcggtgct cagcggcgcg gggatctccg cggaaagcgg tgtaccgacg ttccgcgatg acaagaacgg attgtgggcc cgattcgacc cttacgagct gtccagcacg caaggctggc tgcgcaaccc cgagcgggtc tggggatggt acctatggcg ccattatctg gtggccaacg tcgaacccaa cgacgggcat cgcgcgatcg ccgcctggca ggaccatgct gaggtcagcg tcatcaccca gaatgtcgac gatctgcacg agcgcgccgg cagcggtgcg gtgcatcacc tgcacggcag ccttttcgaa tttcgttgtg cacgttgtgg tgtgccctac accgacgccc ttccggagat gcccgagcct gcgatcgaag tggagccgcc ggtctgcgac tgcggcggtc tgatccggcc cgacatcgta tggttcggtg agccgctgcc agaggagccg tggcggagcg cggtcgaggc gacagggagc gccgacgtga tggtcgtggt ggggacctcg gcgatcgtct acccggcggc cggtttaccc gacctggcgc tggcgcgcgg cactgccgtg atcgaagtca atcccgagcc cacgccgttg tccggcagcg cgacgatcag catccgcgag tcggccagcc aagcgttgcc ggggctgttg gagcgcctgc ccgccctgct gaaatgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat
Details for MytuD.00693.a.B1.GE27168
HARVESTED ON: 7/20/2009
SEQUENCED ON: 7/28/2009
EXPECTED MW: 26kDa
OBSERVED MW: 26kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMR VAVLSGAGIS AESGVPTFRD DKNGLWARFD PYELSSTQGW LRNPERVWGW YLWRHYLVAN VEPNDGHRAI AAWQDHAEVS VITQNVDDLH ERAGSGAVHH LHGSLFEFRC ARCGVPYTDA LPEMPEPAIE VEPPVCDCGG LIRPDIVWFG EPLPEEPWRS AVEATGSADV MVVVGTSAIV YPAAGLPDLA LARGTAVIEV NPEPTPLSGS ATISIRESAS QALPGLLERL PALLK
Validated NT Sequence
tcttactcac ttatttcagc agggcgggca ggcgctccaa cagccccggc aacgcttggc tggccgactc gcggatgctg atcgtcgcgc tgccggacaa cggcgtgggc tcgggattga cttcgatcac ggcagtgccg cgcgccagcg ccaggtcggg taaaccggcc gccgggtaga cgatcgccga ggtccccacc acgaccatca cgtcggcgct ccctgtcgcc tcgaccgcgc tccgccacgg ctcctctggc agcggctcac cgaaccatac gatgtcgggc cggatcagac cgccgcagtc gcagaccggc ggctccactt cgatcgcagg ctcgggcatc tccggaaggg cgtcggtgta gggcacacca caacgtgcac aacgaaattc gaaaaggctg ccgtgcaggt gatgcaccgc accgctgccg gcgcgctcgt gcagatcgtc gacattctgg gtgatgacgc tgacctcagc atggtcctgc caggcggcga tcgcgcgatg cccgtcgttg ggttcgacgt tggccaccag ataatggcgc cataggtacc atccccagac ccgctcgggg ttgcgcagcc agccttgcgt gctggacagc tcgtaagggt cgaatcgggc ccacaatccg ttcttgtcat cgcggaacgt cggtacaccg ctttccgcgg agatccccgc gccgctgagc accgccactc gcatatggtg gtggtggtgg tgagccat
Expected Protein Sequence
MAHHHHHHMR VAVLSGAGIS AESGVPTFRD DKNGLWARFD PYELSSTQGW LRNPERVWGW YLWRHYLVAN VEPNDGHRAI AAWQDHAEVS VITQNVDDLH ERAGSGAVHH LHGSLFEFRC ARCGVPYTDA LPEMPEPAIE VEPPVCDCGG LIRPDIVWFG EPLPEEPWRS AVEATGSADV MVVVGTSAIV YPAAGLPDLA LARGTAVIEV NPEPTPLSGS ATISIRESAS QALPGLLERL PALLK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcgag tggcggtgct cagcggcgcg gggatctccg cggaaagcgg tgtaccgacg ttccgcgatg acaagaacgg attgtgggcc cgattcgacc cttacgagct gtccagcacg caaggctggc tgcgcaaccc cgagcgggtc tggggatggt acctatggcg ccattatctg gtggccaacg tcgaacccaa cgacgggcat cgcgcgatcg ccgcctggca ggaccatgct gaggtcagcg tcatcaccca gaatgtcgac gatctgcacg agcgcgccgg cagcggtgcg gtgcatcacc tgcacggcag ccttttcgaa tttcgttgtg cacgttgtgg tgtgccctac accgacgccc ttccggagat gcccgagcct gcgatcgaag tggagccgcc ggtctgcgac tgcggcggtc tgatccggcc cgacatcgta tggttcggtg agccgctgcc agaggagccg tggcggagcg cggtcgaggc gacagggagc gccgacgtga tggtcgtggt ggggacctcg gcgatcgtct acccggcggc cggtttaccc gacctggcgc tggcgcgcgg cactgccgtg atcgaagtca atcccgagcc cacgccgttg tccggcagcg cgacgatcag catccgcgag tcggccagcc aagcgttgcc ggggctgttg gagcgcctgc ccgccctgct gaaatgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat