SSGCID: Target Page

MyavA.00693.a

NAD-dependent deacetylase

CENTER ID:	MyavA.00693.a
ORGANISM:	Mycobacterium avium 104
ASSOCIATED DISEASE:	Johne's disease, paratuberculosis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyavA.00693.a.A1.GE34916	Full length( MyavA.00693.a )		1	233

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|243243.7.peg.1262
RefSeq:	YP_880534.1
UniProt:	A0QC96

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MLSGAGISAE SGVPTFRDDK NGLWARFDPY ELSSTQGWRD NPQRVWGWYL WRHYLVADVA PNAGHRAIAA WQDHAEVSVI TQNVDDLHER AGSRPVHHLH GSLFEFRCAR CAKPYTGELP AMAEPALEVQ PPVCGCGGLI RPDIVWFGEQ LPDEPWRRAV EATESADVVV VVGTSAIVYP AAGLAELALS RGAAVVEVNP EVTPLSASAT LSIRETASQA LPGLLQRLPA LLN

NT Sequence

GTGCTCAGCG GCGCGGGCAT CTCCGCGGAG AGCGGGGTGC CGACCTTTCG CGACGACAAG AACGGACTGT GGGCCCGCTT CGACCCCTAC GAGCTGTCCA GCACCCAGGG GTGGCGCGAC AACCCGCAGC GCGTCTGGGG CTGGTATCTG TGGCGTCACT ACCTGGTGGC CGACGTCGCG CCGAACGCGG GTCATCGCGC GATCGCCGCC TGGCAAGACC ACGCCGAGGT CAGCGTCATC ACCCAGAACG TCGACGACCT GCACGAGCGG GCCGGTAGCC GGCCCGTGCA CCACCTGCAC GGCAGCCTGT TCGAATTCCG TTGCGCCCGT TGCGCAAAGC CCTACACCGG CGAGCTGCCG GCGATGGCCG AACCGGCGCT GGAGGTGCAA CCCCCGGTGT GCGGATGCGG CGGCCTGATC AGGCCCGACA TCGTGTGGTT CGGCGAACAG CTGCCCGACG AGCCGTGGCG GCGTGCCGTC GAGGCGACCG AGTCCGCCGA CGTGGTGGTG GTGGTGGGCA CCTCGGCGAT CGTGTATCCG GCGGCCGGGC TGGCCGAGCT GGCGTTGTCC CGCGGCGCCG CCGTCGTCGA GGTCAATCCC GAGGTCACGC CGCTGTCGGC CAGCGCCACG CTCAGCATCC GGGAGACGGC CAGCCAGGCC TTGCCCGGGC TGCTGCAGCG GTTGCCGGCG CTGCTCAAC

Details for MyavA.00693.a.A1.GE34916

HARVESTED ON:	4/17/2012
SEQUENCED ON:	4/17/2012
EXPECTED MW:	27kDa
OBSERVED MW:	27kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMVLSGAGIS AESGVPTFRD DKNGLWARFD PYELSSTQGW RDNPQRVWGW YLWRHYLVAD VAPNAGHRAI AAWQDHAEVS VITQNVDDLH ERAGSRPVHH LHGSLFEFRC ARCAKPYTGE LPAMAEPALE VQPPVCGCGG LIRPDIVWFG EQLPDEPWRR AVEATESADV VVVVGTSAIV YPAAGLAELA LSRGAAVVEV NPEVTPLSAS ATLSIRETAS QALPGLLQRL XALLN

Validated NT Sequence

atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggtgc tcagcggcgc gggcatctcc gcggagagcg gggtgccgac ctttcgcgac gacaagaacg gactgtgggc ccgcttcgac ccctacgagc tgtccagcac ccaggggtgg cgcgacaacc cgcagcgcgt ctggggctgg tatctgtggc gtcactacct ggtggccgac gtcgcgccga acgcgggtca tcgcgcgatc gccgcctggc aagaccacgc cgaggtcagc gtcatcaccc agaacgtcga cgacctgcac gagcgggccg gtagccggcc cgtgcaccac ctgcacggca gcctgttcga attccgttgc gcccgttgcg caaagcccta caccggcgag ctgccggcga tggccgaacc ggcgctggag gtgcaacccc cggtgtgcgg atgcggcggc ctgatcaggc ccgacatcgt gtggttcggc gaacagctgc ccgacgagcc gtggcggcgt gccgtcgagg cgaccgagtc cgccgacgtg gtggtggtgg tgggcacctc ggcgatcgtg tatccggcgg ccgggctggc cgagctggcg ttgtcccgcg gcgccgccgt cgtcgaggtc aatcccgagg tcacgccgct gtcggccagc gccacgctca gcatccggga gacggccagc caggccttgc ccgggctgct gcagcggttg nnngcgctgc tcaactaata a

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgctcagc ggcgcgggca tctccgcgga gagcggggtg ccgacctttc gcgacgacaa gaacggactg tgggcccgct tcgaccccta cgagctgtcc agcacccagg ggtggcgcga caacccgcag cgcgtctggg gctggtatct gtggcgtcac tacctggtgg ccgacgtcgc gccgaacgcg ggtcatcgcg cgatcgccgc ctggcaagac cacgccgagg tcagcgtcat cacccagaac gtcgacgacc tgcacgagcg ggccggtagc cggcccgtgc accacctgca cggcagcctg ttcgaattcc gttgcgcccg ttgcgcaaag ccctacaccg gcgagctgcc ggcgatggcc gaaccggcgc tggaggtgca acccccggtg tgcggatgcg gcggcctgat caggcccgac atcgtgtggt tcggcgaaca gctgcccgac gagccgtggc ggcgtgccgt cgaggcgacc gagtccgccg acgtggtggt ggtggtgggc acctcggcga tcgtgtatcc ggcggccggg ctggccgagc tggcgttgtc ccgcggcgcc gccgtcgtcg aggtcaatcc cgaggtcacg ccgctgtcgg ccagcgccac gctcagcatc cgggagacgg ccagccaggc cttgcccggg ctgctgcagc ggttgccggc gctgctcaac aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa