SSGCID: Target Page

MyabA.00693.a

NAD-dependent deacetylase (Regulatory protein Sir2 homolog)

CENTER ID:	MyabA.00693.a
ORGANISM:	Mycobacterium abscessus ATCC 19977 / DSM 44196
ASSOCIATED DISEASE:	Skin abscess
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyabA.00693.a.A1.GE30335	Full length( MyabA.00693.a )		1	248

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|36809.5.peg.3505
RefSeq:	YP_001704171.1
UniProt:	B1MER1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTEVPDELIA AASKARSVTV LTGAGMSAES GLPTFRDAQT GLWSKYDPMT LATPESWQED PGLVWAWYQN RRIQLLAVQP NDGHRALAQW GSHRDIRIVT QNVDDLHERA GSSNVTHVHG SLLKSHCDTC RSDFEATVSA PEAERVAPPE CGCGGKVRPS IVWFGEMLPE VEFGHAVAHS QNCDLMLLIG TSGIVYPAAG LPQLALSRGA TVVEINPQET DLSDRADLVW RSTAATALPA LLQALAPF

NT Sequence

ATGACCGAGG TTCCCGACGA GCTGATCGCC GCCGCGTCGA AGGCACGTTC CGTCACCGTG CTTACTGGCG CCGGCATGTC CGCCGAGAGC GGGTTGCCCA CGTTCCGCGA TGCGCAGACG GGGCTGTGGT CGAAGTATGA CCCGATGACC CTTGCCACCC CGGAATCATG GCAAGAGGAC CCGGGGCTGG TGTGGGCGTG GTATCAGAAT CGGCGCATCC AGCTGCTGGC GGTACAACCC AACGACGGGC ATCGGGCGTT GGCGCAGTGG GGATCTCATC GCGACATTCG GATCGTCACC CAGAACGTCG ATGATCTTCA CGAACGTGCC GGCAGTTCGA ATGTCACGCA CGTTCACGGC AGCCTGCTCA AATCGCACTG CGACACCTGC CGCAGTGATT TCGAGGCAAC GGTTTCCGCC CCCGAAGCGG AGCGCGTGGC CCCGCCGGAG TGCGGTTGCG GGGGAAAGGT CCGCCCGAGC ATCGTGTGGT TCGGGGAGAT GCTTCCCGAG GTCGAGTTCG GTCATGCGGT AGCGCATTCG CAGAATTGCG ATCTGATGTT GCTCATCGGC ACCTCCGGGA TCGTGTATCC GGCGGCAGGG CTCCCGCAAT TGGCTCTGAG TCGTGGCGCG ACCGTGGTCG AGATCAACCC GCAGGAGACC GACCTGTCCG ATCGCGCGGA CCTGGTTTGG AGATCCACTG CGGCCACGGC ACTGCCTGCG CTCCTCCAGG CGCTGGCACC GTTC

Details for MyabA.00693.a.A1.GE30335

HARVESTED ON:	9/8/2010
SEQUENCED ON:	9/17/2010
EXPECTED MW:	29kDa
OBSERVED MW:	29kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMTEVPDELI AAASKARSVT VLTGAGMSAE SGLPTFRDAQ TGLWSKYDPM TLATPESWQE DPGLVWAWYQ NRRIQLLAVQ PNDGHRALAQ WGSHRDIRIV TQNVDDLHER AGSSNVTHVH GSLLKSHCDT CRSDFEATVS APEAERVAPP ECGCGGKVRP SIVWFGEMLP EVEFGHAVAH SQNCDLMLLI GTSGIVYPAA GLPQLALSRG ATVVEINPXE TDLSDRADLV WRSTAATALP ALLQALAPF

Validated NT Sequence

attttgttta ctttaagaag gagatatacc atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgaccg aggttcccga cgagctgatc gccgccgcgt cgaaggcacg ttccgtcacc gtgcttactg gcgccggcat gtccgccgag agcgggttgc ccacgttccg cgatgcgcag acggggctgt ggtcgaagta tgacccgatg acccttgcca ccccggaatc atggcaagag gacccggggc tggtgtgggc gtggtatcag aatcggcgca tccagctgct ggcggtacaa cccaacgacg ggcatcgggc gttggcgcag tggggatctc atcgcgacat tcggatcgtc acccagaacg tcgatgatct tcacgaacgt gccggcagtt cgaatgtcac gcacgttcac ggcagcctgc tcaaatcgca ctgcgacacc tgccgcagtg atttcgaggc aacggtttcc gcccccgaag cggagcgcgt ggccccgccg gagtgcggtt gcgggggaaa ggtccgcccg agcatcgtgt ggttcgggga gatgcttccc gaggtcgagt tcggtcatgc ggtagcgcat tcgcagaatt gcgatctgat gttgctcatc ggcacctccg ggatcgtgta tccggcggca gggctcccgc aattggctct gagtcgtggc gcgaccgtgg tcgagatcaa cccgcangag accgacctgt ccgatcgcgc ggacctggtt tggagatcca ctgcggccac ggcactgcct gcgctcctcc aggcgctggc accgttctaa taaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctganttggc tgctgccacc gct

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaccgaggtt cccgacgagc tgatcgccgc cgcgtcgaag gcacgttccg tcaccgtgct tactggcgcc ggcatgtccg ccgagagcgg gttgcccacg ttccgcgatg cgcagacggg gctgtggtcg aagtatgacc cgatgaccct tgccaccccg gaatcatggc aagaggaccc ggggctggtg tgggcgtggt atcagaatcg gcgcatccag ctgctggcgg tacaacccaa cgacgggcat cgggcgttgg cgcagtgggg atctcatcgc gacattcgga tcgtcaccca gaacgtcgat gatcttcacg aacgtgccgg cagttcgaat gtcacgcacg ttcacggcag cctgctcaaa tcgcactgcg acacctgccg cagtgatttc gaggcaacgg tttccgcccc cgaagcggag cgcgtggccc cgccggagtg cggttgcggg ggaaaggtcc gcccgagcat cgtgtggttc ggggagatgc ttcccgaggt cgagttcggt catgcggtag cgcattcgca gaattgcgat ctgatgttgc tcatcggcac ctccgggatc gtgtatccgg cggcagggct cccgcaattg gctctgagtc gtggcgcgac cgtggtcgag atcaacccgc aggagaccga cctgtccgat cgcgcggacc tggtttggag atccactgcg gccacggcac tgcctgcgct cctccaggcg ctggcaccgt tctaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa