SSGCID: Target Page

MysmA.00693.a

NAD-dependent deacetylase

CENTER ID:	MysmA.00693.a
ORGANISM:	Mycobacterium smegmatis ATCC 700084 / mc(2)155
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MysmA.00693.a.A1.GE28829	Full length( MysmA.00693.a )		1	240

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|246196.19.peg.5049
RefSeq:	YP_889421.1
UniProt:	A0R2N3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MQVTVLSGAG ISAESGVPTF RDAETGLWAQ VDPYEISSTD GWQRNPEKVW AWYLWRHYMM ARVAPNEAHR TVAAWEDHLD VRVVTQNIDD LHERAGSTNV YHLHGSLFEF RCDACGSAFE GNLPEMPEPV ETIDPPVCPC SGLIRPSVVW FGEPLPDAAW NRSVLAVSSA DVVIVVGTSS IVYPAAGLPE AALAAGKPVI EVNPERTPLS DSATVSLRET ASEALPTLLQ RLPELLNRSA

NT Sequence

GTGCAAGTTA CTGTGCTCAG CGGCGCCGGG ATCTCGGCAG AAAGCGGCGT GCCCACGTTC CGCGACGCCG AGACCGGCCT GTGGGCCCAG GTCGACCCGT ACGAGATCTC CAGCACCGAC GGTTGGCAGC GCAACCCCGA GAAGGTGTGG GCCTGGTATC TGTGGCGGCA CTACATGATG GCGCGGGTCG CGCCCAACGA AGCGCACCGC ACCGTGGCCG CCTGGGAGGA CCACCTCGAC GTGCGCGTCG TCACCCAGAA CATCGACGAT CTGCACGAGC GCGCGGGCAG CACCAATGTG TACCACCTGC ACGGCAGCCT GTTCGAATTC CGTTGTGACG CTTGCGGGTC TGCGTTCGAG GGCAACCTCC CCGAGATGCC GGAACCGGTC GAGACCATCG ACCCGCCGGT GTGCCCGTGC AGCGGCCTGA TCCGTCCCAG CGTGGTGTGG TTCGGCGAGC CGCTGCCCGA CGCCGCCTGG AATCGCTCGG TGCTCGCGGT GAGCAGCGCC GACGTGGTGA TCGTGGTCGG CACGAGTTCG ATCGTCTACC CGGCCGCCGG TCTCCCCGAG GCGGCCCTGG CCGCGGGAAA GCCCGTCATC GAGGTCAACC CCGAGCGCAC GCCGCTGTCC GACAGCGCCA CGGTCTCGTT GCGCGAGACC GCCAGCGAGG CACTTCCGAC GTTGTTGCAG CGCCTGCCCG AACTGCTCAA CCGCTCGGCC

Details for MysmA.00693.a.A1.GE28829

HARVESTED ON:	3/23/2010
SEQUENCED ON:	4/13/2010
EXPECTED MW:	28kDa
OBSERVED MW:	28kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	97
PERCENT COVERAGE:	91

Validated AA Sequence

MVQVTVLSGA GISAESGVPT FRDAETGLWA QVDPYEISST DGWQRNPEKV WAWYLWRHYM MARVAPNEAH RTVAAWEDHL DVRVVTQNID DLHERAGSTN VYHLHGSLFE FRCDACGSAF EGNLPEMPEP VETIDPPVCP CSGLIRPSVX XXGEPLPDAA WNRSVLAVSS ADVVIVVGTS SIVYPAXXLP EAALAAGKPV IEVNPERTPL SDSATVSLRE TASEALPTLX QRLPELLNRS A

Validated NT Sequence

taggccgagc ggttgagcag ttcgggnagg cgctgcanca acgtcggaag tgcctcgctg gcggtctcgc gcaacgagac cgtggcgctg tcggacagcg gcgtgcgctc ggggttgacc tcgatgacgg gctttcccgc ggccagggcc gcctcgggga gacnggnggc cgggtagacg atcgaactcg tgccgaccac gatcaccacg tcggcgctgc tcaccgcgag caccgagcga ttccaggcgg cgtcgggcag cggctcgccg anccncncca cgctgggacg gatcaggccg ctgcacgggc acaccggcgg gtcgatggtc tcgaccggtt ccggcatctc ggggaggttg ccctcgaacg cagacccgca agcgtcacaa cggaattcga acaggctgcc gtgcaggtgg tacacattgg tgctgcccgc gcgctcgtgc agatcgtcga tgttctgggt gacgacgcgc acgtcgaggt ggtcctccca ggcggccacg gtgcggtgcg cttcgttggg cgcgacccgc gccatcatgt agtgccgcca cagataccag gcccacacct tctcggggtt gcgctgccaa ccgtcggtgc tggagatctc gtacgggtcg acctgggccc acaggccggt ctcggcgtcg cggaacgtgg gcacgccgct ttctgccgag atcccggcgc cgctgagcac agtaacttgc accatcgaac caggaccctg ggtctgagct tccagggta

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMVQVTVLSG AGISAESGVP TFRDAETGLW AQVDPYEISS TDGWQRNPEK VWAWYLWRHY MMARVAPNEA HRTVAAWEDH LDVRVVTQNI DDLHERAGST NVYHLHGSLF EFRCDACGSA FEGNLPEMPE PVETIDPPVC PCSGLIRPSV VWFGEPLPDA AWNRSVLAVS SADVVIVVGT SSIVYPAAGL PEAALAAGKP VIEVNPERTP LSDSATVSLR ETASEALPTL LQRLPELLNR SA

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgcaagtt actgtgctca gcggcgccgg gatctcggca gaaagcggcg tgcccacgtt ccgcgacgcc gagaccggcc tgtgggccca ggtcgacccg tacgagatct ccagcaccga cggttggcag cgcaaccccg agaaggtgtg ggcctggtat ctgtggcggc actacatgat ggcgcgggtc gcgcccaacg aagcgcaccg caccgtggcc gcctgggagg accacctcga cgtgcgcgtc gtcacccaga acatcgacga tctgcacgag cgcgcgggca gcaccaatgt gtaccacctg cacggcagcc tgttcgaatt ccgttgtgac gcttgcgggt ctgcgttcga gggcaacctc cccgagatgc cggaaccggt cgagaccatc gacccgccgg tgtgcccgtg cagcggcctg atccgtccca gcgtggtgtg gttcggcgag ccgctgcccg acgccgcctg gaatcgctcg gtgctcgcgg tgagcagcgc cgacgtggtg atcgtggtcg gcacgagttc gatcgtctac ccggccgccg gtctccccga ggcggccctg gccgcgggaa agcccgtcat cgaggtcaac cccgagcgca cgccgctgtc cgacagcgcc acggtctcgt tgcgcgagac cgccagcgag gcacttccga cgttgttgca gcgcctgccc gaactgctca accgctcggc caaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa