MytuD.00663.a

cytidylate kinase

CENTER ID: MytuD.00663.a
ORGANISM: Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE: Tuberculosis
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MytuD.00663.a.A1.GE25867 Full length( MytuD.00663.a ) 1 230
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
OrthoMCL: OG5_133121
PATRIC ID: fig|83332.12.peg.1905
RefSeq: NP_216228.1
TubercuList: Rv1712
MTB Network Portal: Rv1712
UniProt: P9WPA9

Orthologues for Rv1712

Species % Identity % Coverage Clones Proteins Structures
paratuberculosis 79 98 1 2 0
marinum 79 97 1 1 0
avium 78 97 0 0 0
ulcerans 78 97 1 1 0
leprae 75 97 1 0 0
smegmatis 74 97 1 0 1
abscessus 68 97 1 0 2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSRLSAAVVA IDGPAGTGKS SVSRRLAREL GARFLDTGAM YRIVTLAVLR AGADPSDIAA VETIASTVQM SLGYDPDGDS CYLAGEDVSV EIRGDAVTRA VSAVSSVPAV RTRLVELQRT MAEGPGSIVV EGRDIGTVVF PDAPVKIFLT ASAETRARRR NAQNVAAGLA DDYDGVLADV RRRDHLDSTR AVSPLQAAGD AVIVDTSDMT EAEVVAHLLE LVTRRSEAVR
NT Sequence
GTGAGTCGCC TAAGCGCAGC GGTAGTCGCG ATCGACGGGC CGGCCGGCAC CGGAAAATCC TCGGTGTCAA GGCGATTAGC GCGCGAGCTG GGCGCACGCT TTCTGGACAC CGGGGCAATG TATCGGATCG TGACGTTGGC GGTGCTGCGT GCCGGTGCTG ATCCGTCCGA TATCGCTGCC GTCGAGACGA TTGCGTCGAC GGTGCAGATG TCGTTAGGCT ACGATCCCGA CGGAGACAGC TGTTACCTTG CCGGAGAAGA CGTTTCGGTT GAGATACGCG GTGACGCGGT CACCCGTGCG GTCTCCGCGG TGTCGTCGGT GCCGGCCGTA CGCACCCGGC TGGTCGAGCT GCAGCGAACA ATGGCTGAGG GCCCGGGCAG CATCGTCGTG GAGGGCCGCG ACATCGGAAC CGTGGTGTTT CCGGATGCGC CGGTGAAAAT CTTCTTGACC GCCTCGGCCG AAACGCGGGC CCGGCGGCGC AACGCCCAAA ACGTCGCGGC GGGTTTGGCC GACGACTATG ACGGGGTATT GGCCGATGTG CGCCGGCGCG ACCACCTCGA TTCCACCCGG GCGGTGTCAC CGCTGCAAGC CGCCGGTGAT GCCGTCATCG TGGACACCAG CGATATGACC GAGGCCGAGG TGGTCGCCCA TCTGTTGGAG CTGGTCACGC GGCGAAGTGA GGCAGTGCGG
Details for MytuD.00663.a.A1.GE25867
HARVESTED ON: 11/19/2008
SEQUENCED ON: 8/18/2010
EXPECTED MW: 26kDa
OBSERVED MW: 26kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVSRLSAAV VAIDGPAGTG KSSVSRRLAR ELGARFLDTG AMYRIVTLAV LRAGADPSDI AAVETIASTV QMSLGYDPDG DSCYLAGEDV SVEIRGDAVT RAVSAVSSVP AVRTRLVELQ RTMAEGPGSI VVEGRDIGTV VFPDAPVKIF LTASAETRAR RRNAQNVAAG LADDYDGVLA DVRRRDHLDS TRAVSPLQAA GDAVIVDTSD MTEAEVVAHL LELVTRRSEA VR
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggtga gtcgcctaag cgcagcggta gtcgcgatcg acgggccggc cggcaccgga aaatcctcgg tgtcaaggcg attagcgcgc gagctgggcg cacgctttct ggacaccggg gcaatgtatc ggatcgtgac gttggcggtg ctgcgtgccg gtgctgatcc gtccgatatc gctgccgtcg agacgattgc gtcgacggtg cagatgtcgt taggctacga tcccgacgga gacagctgtt accttgccgg agaagacgtt tcggttgaga tacgcggtga cgcggtcacc cgtgcggtct ccgcggtgtc gtcggtgccg gccgtacgca cccggctggt cgagctgcag cgaacaatgg ctgagggccc gggcagcatc gtcgtggagg gccgcgacat cggaaccgtg gtgtttccgg atgcgccggt gaaaatcttc ttgaccgcct cggccgaaac gcgggcccgg cggcgcaacg cccaaaacgt cgcggcgggt ttggccgacg actatgacgg ggtattggcc gatgtgcgcc ggcgcgacca cctcgattcc acccgggcgg tgtcaccgct gcaagccgcc ggtgatgccg tcatcgtgga caccagcgat atgaccgagg ccgaggtggt cgcccatctg ttggagctgg tcacgcggcg aagtgaggca gtgcggtaat aa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVSRLSAAV VAIDGPAGTG KSSVSRRLAR ELGARFLDTG AMYRIVTLAV LRAGADPSDI AAVETIASTV QMSLGYDPDG DSCYLAGEDV SVEIRGDAVT RAVSAVSSVP AVRTRLVELQ RTMAEGPGSI VVEGRDIGTV VFPDAPVKIF LTASAETRAR RRNAQNVAAG LADDYDGVLA DVRRRDHLDS TRAVSPLQAA GDAVIVDTSD MTEAEVVAHL LELVTRRSEA VR
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgagtcgc ctaagcgcag cggtagtcgc gatcgacggg ccggccggca ccggaaaatc ctcggtgtca aggcgattag cgcgcgagct gggcgcacgc tttctggaca ccggggcaat gtatcggatc gtgacgttgg cggtgctgcg tgccggtgct gatccgtccg atatcgctgc cgtcgagacg attgcgtcga cggtgcagat gtcgttaggc tacgatcccg acggagacag ctgttacctt gccggagaag acgtttcggt tgagatacgc ggtgacgcgg tcacccgtgc ggtctccgcg gtgtcgtcgg tgccggccgt acgcacccgg ctggtcgagc tgcagcgaac aatggctgag ggcccgggca gcatcgtcgt ggagggccgc gacatcggaa ccgtggtgtt tccggatgcg ccggtgaaaa tcttcttgac cgcctcggcc gaaacgcggg cccggcggcg caacgcccaa aacgtcgcgg cgggtttggc cgacgactat gacggggtat tggccgatgt gcgccggcgc gaccacctcg attccacccg ggcggtgtca ccgctgcaag ccgccggtga tgccgtcatc gtggacacca gcgatatgac cgaggccgag gtggtcgccc atctgttgga gctggtcacg cggcgaagtg aggcagtgcg gaaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa