MysmA.00663.a
Cytidylate kinase
| CENTER ID: | MysmA.00663.a |
| ORGANISM: | Mycobacterium smegmatis ATCC 700084 / mc(2)155 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIC |
Ordering Clones & Proteins
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MysmA.00663.a.A1.GE28823 | Full length( MysmA.00663.a ) | 1 | 228 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Structures
3R20
DEPOSITED: 3/11/2011
DETERMINATION: XRay
CLONE: MysmA.00663.a.A1.GE28823
PROTEIN: MysmA.00663.a.A1.PS00600
Publications by SSGCID
Mycobacterium Cytidylate Kinase Appears to Be an Undruggable Target
Baker D, Barrett LK, Dong W, Risler JK, Samudrala R, Subramanian S, Van Voorhis WC, Craig JC, Loesch K
J Biomol Screen - 2016
volume 21, issue 7, pages 695-700
PMID: 27146385; PMCID: PMC8565994
PDB:
3R20
Increasing the structural coverage of tuberculosis drug targets.
Abendroth J, Abramov A, Armour B, Barrett LK, Baugh L, Begley DW, Buchko GW, Choi R, Clifton MC, Dieterich SH, Dranow DM, Edwards TE, Fairman JW, Ferrell M, Fox D, Gardberg AS, Hewitt SN, Lorimer D, Lyons-Abbott S, Mundt E, Muruthi MM, Myers J, Myler PJ, Napuli AJ, Phan I, Sekar A, Serbzhinskiy D, Stacy R, Staker BL, Stewart LJ, Taylor BM, Thompkins K, Tran N, Van Voorhis WC, Zhang Y
Tuberculosis (Edinb) - 2014
volume 95, issue 2, pages 142-8
PMID: 25613812; PMCID: PMC4361283
PDB:
3GVC,
3GVG,
3GWC,
3H7F,
3H81,
3HE2,
3HWI,
3HWK,
3HZG,
3ICO,
3KHP,
3LLS,
3MOY,
3MPZ,
3MYB,
3NDN,
3NDO,
3NF4,
3NG3,
3NJD,
3NWO,
3O0M,
3O38,
3OC6,
3OC7,
3OEC,
3OI9,
3OKS,
3OL3,
3OL4,
3OME,
3P0T,
3P2Y,
3P4I,
3P4T,
3P5M,
3P85,
3PE8,
3PGX,
3PK0,
3PPI,
3PXX,
3PZY,
3Q1T,
3Q8N,
3QBP,
3QDF,
3QH4,
3QHA,
3QIV,
3QK8,
3QKA,
3QLJ,
3QMJ,
3QRE,
3QUA,
3QUV,
3QXI,
3QXZ,
3QYR,
3R0O,
3R1I,
3R1J,
3R20,
3R2N,
3R4T,
3R6H,
3R6O,
3R7K,
3R8C,
3R9P,
3R9Q,
3R9R,
3R9S,
3R9T,
3RD5,
3RD7,
3RD8,
3RFQ,
3RIH,
3RR2,
3RR6,
3RRP,
3RRV,
3RSI,
3RV2,
3S55,
3S82,
3SBX,
3SF6,
3SLL,
3SVK,
3SVT,
3SWO,
3SWT,
3SWX,
3SX2,
3T3W,
3T7C,
3TAV,
3TCR,
3TDE,
3TJR,
3TL3,
3TLF,
3TRR,
3TSC,
3TX2,
3TZQ,
3TZU,
3U0A,
3U0B,
3UCX,
3UVE,
4DI1,
4DIE,
4DQ8,
4DXL,
4ED4,
4EGE,
4EGF,
4EMD,
4EO9,
4EYE,
4F3W,
4F47,
4FFC,
4GK6,
4HDT,
4HR3,
4I1Y,
4IJN,
4IV6,
4IZ9,
4J5I,
4KAM,
4LGV,
4O2D,
5EJ2
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| BV-BRC: | fig|246196.19.peg.3680 |
| RefSeq: | YP_888038.1 |
| UniProt: | A0QYQ0 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSGSLVVAVD GPAGTGKSSV SRGLARALGA RYLDTGAMYR IATLAVLRAG ADLTDPAAIE KAAADAEIGV GSDPDVDAAF LAGEDVSSEI RGDAVTGAVS AVSAVPAVRT RLVDIQRKLA TEGGRVVVEG RDIGTVVLPD ADVKIFLTAS AEERARRRNA QNVANGLPDD YATVLADVQR RDHLDSTRPV SPLRAADDAL VVDTSDMDQA QVIAHLLDLV TAQAGARR
NT Sequence
GTGAGCGGAA GTTTGGTTGT TGCGGTGGAC GGCCCGGCCG GCACCGGAAA GTCCTCGGTG TCACGGGGGT TGGCCCGCGC GCTCGGCGCC CGGTACCTCG ACACCGGCGC GATGTACCGC ATCGCGACGC TTGCGGTGCT GCGGGCCGGT GCTGACCTCA CCGACCCTGC GGCGATCGAG AAGGCCGCCG CCGACGCCGA GATCGGAGTG GGCTCAGATC CCGATGTCGA CGCGGCATTC CTTGCCGGAG AAGATGTTTC GTCGGAGATC CGCGGTGACG CGGTGACCGG CGCGGTGTCG GCGGTCTCCG CAGTTCCCGC CGTGCGCACA CGCCTGGTCG ACATTCAGCG CAAGCTGGCC ACCGAGGGTG GGCGAGTGGT GGTCGAGGGC CGCGACATCG GCACCGTCGT GCTGCCCGAT GCCGACGTCA AGATCTTCCT CACCGCGTCG GCCGAGGAAC GCGCGCGGCG ACGCAACGCG CAGAACGTCG CCAACGGACT GCCCGACGAC TACGCAACTG TGCTGGCCGA CGTACAGCGT CGCGACCATC TCGACTCGAC CCGTCCCGTG TCGCCGCTGC GCGCCGCCGA CGACGCGCTG GTGGTCGACA CGAGCGACAT GGACCAGGCC CAGGTGATCG CCCACCTGCT CGATCTGGTC ACCGCACAGG CGGGAGCACG TCGA
Details for MysmA.00663.a.A1.GE28823
| HARVESTED ON: | 3/23/2010 |
| SEQUENCED ON: | 4/13/2010 |
| EXPECTED MW: | 25kDa |
| OBSERVED MW: | 27kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVSGSLVVA VDGPAGTGKS SVSRGLARAL GARYLDTGAM YRIATLAVLR AGADLTDPAA IEKAAADAEI GVGSDPDVDA AFLAGEDVSS EIRGDAVTGA VSAVSAVPAV RTRLVDIQRK LATEGGRVVV EGRDIGTVVL PDADVKIFLT ASAEERARRR NAQNVANGLP DDYATVLADV QRRDHLDSTR PVSPLRAADD ALVVDTSDMD QAQVIAHLLD LVTAQAGARR
Validated NT Sequence
ttattatcga cgtgctcccg cctgtgcggt gaccagatcg agcaggtggg cgatcacctg ggcctggtcc atgtcgctcg tgtcgaccac cagcgcgtcg tcggcggcgc gcagcggcga cacgggacgg gtcgagtcga gatggtcgcg acgctgtacg tcggccagca cagttgcgta gtcgtcgggc agtccgttgg cgacgttctg cgcgttgcgt cgccgcgcgc gttcctcggc cgacgcggtg aggaagatct tgacgtcggc atcgggcagc acgacggtgc cgatgtcgcg gccctcgacc accactcgcc caccctcggt ggccagcttg cgctgaatgt cgaccaggcg tgtgcgcacg gcgggaactg cggagaccgc cgacaccgcg ccggtcaccg cgtcaccgcg gatctccgac gaaacatctt ctccggcaag gaatgccgcg tcgacatcgg gatctgagcc cactccgatc tcggcgtcgg cggcggcctt ctcgatcgcc gcagggtcgg tgaggtcagc accggcccgc agcaccgcaa gcgtcgcgat gcggtacatc gcgccggtgt cgaggtaccg ggcgccgagc gcgcgggcca acccccgtga caccgaggac tttccggtgc cggccgggcc gtccaccgca acaaccaaac ttccgctcac catcgaacca ggaccctggg tctgagcttc cagggtaccc atatgatggt gatggtgatg agccat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVSGSLVVA VDGPAGTGKS SVSRGLARAL GARYLDTGAM YRIATLAVLR AGADLTDPAA IEKAAADAEI GVGSDPDVDA AFLAGEDVSS EIRGDAVTGA VSAVSAVPAV RTRLVDIQRK LATEGGRVVV EGRDIGTVVL PDADVKIFLT ASAEERARRR NAQNVANGLP DDYATVLADV QRRDHLDSTR PVSPLRAADD ALVVDTSDMD QAQVIAHLLD LVTAQAGARR
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgagcgga agtttggttg ttgcggtgga cggcccggcc ggcaccggaa agtcctcggt gtcacggggg ttggcccgcg cgctcggcgc ccggtacctc gacaccggcg cgatgtaccg catcgcgacg cttgcggtgc tgcgggccgg tgctgacctc accgaccctg cggcgatcga gaaggccgcc gccgacgccg agatcggagt gggctcagat cccgatgtcg acgcggcatt ccttgccgga gaagatgttt cgtcggagat ccgcggtgac gcggtgaccg gcgcggtgtc ggcggtctcc gcagttcccg ccgtgcgcac acgcctggtc gacattcagc gcaagctggc caccgagggt gggcgagtgg tggtcgaggg ccgcgacatc ggcaccgtcg tgctgcccga tgccgacgtc aagatcttcc tcaccgcgtc ggccgaggaa cgcgcgcggc gacgcaacgc gcagaacgtc gccaacggac tgcccgacga ctacgcaact gtgctggccg acgtacagcg tcgcgaccat ctcgactcga cccgtcccgt gtcgccgctg cgcgccgccg acgacgcgct ggtggtcgac acgagcgaca tggaccaggc ccaggtgatc gcccacctgc tcgatctggt caccgcacag gcgggagcac gtcgaaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa