MyabA.00663.a
Probable cytidine monophosphate kinase
| CENTER ID: | MyabA.00663.a |
| ORGANISM: | Mycobacterium abscessus ATCC 19977 / DSM 44196 |
| ASSOCIATED DISEASE: | Skin abscess |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIC |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MyabA.00663.a.A1.GE30331 | Full length( MyabA.00663.a ) | 1 | 223 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Structures
3R8C
DEPOSITED: 3/23/2011
DETERMINATION: XRay
CLONE: MyabA.00663.a.A1.GE30331
PROTEIN: MyabA.00663.a.A1.PW30208
4DIE
DEPOSITED: 1/30/2012
DETERMINATION: XRay
CLONE: MyabA.00663.a.A1.GE30331
PROTEIN: MyabA.00663.a.A1.PW30208
Publications by SSGCID
Increasing the structural coverage of tuberculosis drug targets.
Abendroth J, Abramov A, Armour B, Barrett LK, Baugh L, Begley DW, Buchko GW, Choi R, Clifton MC, Dieterich SH, Dranow DM, Edwards TE, Fairman JW, Ferrell M, Fox D, Gardberg AS, Hewitt SN, Lorimer D, Lyons-Abbott S, Mundt E, Muruthi MM, Myers J, Myler PJ, Napuli AJ, Phan I, Sekar A, Serbzhinskiy D, Stacy R, Staker BL, Stewart LJ, Taylor BM, Thompkins K, Tran N, Van Voorhis WC, Zhang Y
Tuberculosis (Edinb) - 2014
volume 95, issue 2, pages 142-8
PMID: 25613812; PMCID: PMC4361283
PDB:
3GVC,
3GVG,
3GWC,
3H7F,
3H81,
3HE2,
3HWI,
3HWK,
3HZG,
3ICO,
3KHP,
3LLS,
3MOY,
3MPZ,
3MYB,
3NDN,
3NDO,
3NF4,
3NG3,
3NJD,
3NWO,
3O0M,
3O38,
3OC6,
3OC7,
3OEC,
3OI9,
3OKS,
3OL3,
3OL4,
3OME,
3P0T,
3P2Y,
3P4I,
3P4T,
3P5M,
3P85,
3PE8,
3PGX,
3PK0,
3PPI,
3PXX,
3PZY,
3Q1T,
3Q8N,
3QBP,
3QDF,
3QH4,
3QHA,
3QIV,
3QK8,
3QKA,
3QLJ,
3QMJ,
3QRE,
3QUA,
3QUV,
3QXI,
3QXZ,
3QYR,
3R0O,
3R1I,
3R1J,
3R20,
3R2N,
3R4T,
3R6H,
3R6O,
3R7K,
3R8C,
3R9P,
3R9Q,
3R9R,
3R9S,
3R9T,
3RD5,
3RD7,
3RD8,
3RFQ,
3RIH,
3RR2,
3RR6,
3RRP,
3RRV,
3RSI,
3RV2,
3S55,
3S82,
3SBX,
3SF6,
3SLL,
3SVK,
3SVT,
3SWO,
3SWT,
3SWX,
3SX2,
3T3W,
3T7C,
3TAV,
3TCR,
3TDE,
3TJR,
3TL3,
3TLF,
3TRR,
3TSC,
3TX2,
3TZQ,
3TZU,
3U0A,
3U0B,
3UCX,
3UVE,
4DI1,
4DIE,
4DQ8,
4DXL,
4ED4,
4EGE,
4EGF,
4EMD,
4EO9,
4EYE,
4F3W,
4F47,
4FFC,
4GK6,
4HDT,
4HR3,
4I1Y,
4IJN,
4IV6,
4IZ9,
4J5I,
4KAM,
4LGV,
4O2D,
5EJ2
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_133121 |
| PATRIC ID: | fig|36809.5.peg.2414 |
| RefSeq: | YP_001703106.1 |
| UniProt: | B1MB31 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MVAVDGPSGT GKSSVAKELA RQLGASYLDT GAMYRIVTLW VLRAGVDLTD PAAIAAATDQ VPMSVSSDPD AQTALLAGED VSVPIRGNEV TGAVSAVSAV PAVRERLVRQ QRELAESSGA VVVEGRDIGT VVLPDADVKI YLTASAQARA QRRNAQNVSG GGDDEYEKVL ADVQRRDHLD STRAVSPLRP AEDALEVDTS DMTQEQVVAH LLDLVRTRAG ASR
NT Sequence
GTGGTTGCCG TAGATGGCCC ATCCGGTACC GGAAAGTCCT CCGTCGCAAA AGAATTGGCT CGTCAGCTAG GGGCCTCATA TCTCGATACC GGTGCGATGT ACCGGATCGT GACGCTCTGG GTGCTGCGTG CCGGTGTAGA CCTCACCGAC CCCGCGGCCA TCGCCGCGGC AACCGATCAG GTGCCCATGT CCGTGAGTTC GGATCCCGAT GCGCAGACGG CTCTGCTTGC GGGTGAAGAT GTTTCGGTCC CAATCCGGGG TAATGAGGTG ACCGGCGCGG TCTCCGCCGT GTCCGCGGTA CCGGCCGTCC GTGAACGACT GGTGCGGCAG CAGCGCGAGC TGGCCGAGTC CAGTGGCGCC GTGGTGGTGG AGGGCCGTGA TATCGGCACC GTGGTGCTGC CGGACGCCGA CGTGAAGATC TATCTGACCG CATCGGCACA GGCGCGGGCG CAGCGCCGCA ACGCGCAGAA CGTCTCCGGT GGTGGCGACG ACGAATATGA AAAGGTGCTT GCCGATGTGC AGCGCCGTGA CCATCTGGAT TCCACCCGGG CCGTCTCGCC GCTGCGCCCG GCCGAGGATG CTTTAGAGGT CGATACCAGC GATATGACAC AGGAACAGGT CGTGGCCCAC CTGCTGGACC TGGTGCGCAC TAGGGCAGGG GCATCACGA
Details for MyabA.00663.a.A1.GE30331
| HARVESTED ON: | 9/8/2010 |
| SEQUENCED ON: | 9/17/2010 |
| EXPECTED MW: | 25kDa |
| OBSERVED MW: | 30kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVVAVDGPS GTGKSSVAKE LARQLGASYL DTGAMYRIVT LWVLRAGVDL TDPAAIAAAT DQVPMSVSSD PDAQTALLAG EDVSVPIRGN EVTGAVSAVS AVPAVRERLV RQQRELAESS GAVVVEGRDI GTVVLPDADV KIYLTASAQA RAQRRNAQNV SGGGDDEYEK VLADVQRRDH LDSTRAVSPL RPAEDALEVD TSDMTQEQVV AHLLDLVRTR AGASR
Validated NT Sequence
ttattatcgt gatgcccctg ccctagtgcg caccaggtcc agcaggtggg ccacgacctg ttcctgtgtc atatcgctgg tatcgacctc taaagcatcc tcggccgggc gcagcggcga gacggcccgg gtggaatcca gatggtcacg gcgctgcaca tcggcaagca ccttttcata ttcgtcgtcg ccaccaccgg agacgttctg cgcgttgcgg cgctgcgccc gcgcctgtgc cgatgcggtc agatagatct tcacgtcggc gtccggcagc accacggtgc cgatatcacg gccctccacc accacggcgc cactggactc ggccagctcg cgctgctgcc gcaccagtcg ttcacggacg gccggtaccg cggacacggc ggagaccgcg ccggtcacct cattaccccg gattgggacc gaaacatctt cacccgcaag cagagccgtc tgcgcatcgg gatccgaact cacggacatg ggcacctgat cggttgccgc ggcgatggcc gcggggtcgg tgaggtctac accggcacgc agcacccaga gcgtcacgat ccggtacatc gcaccggtat cgagatatga ggcccctagc tgacgagcca attcttttgc gacggaggac tttccggtac cggatgggcc atctacggca accaccatcg aaccaggacc ctgggtctga gcttccaggg tacccatatg atggtgatgg tgatgagcca t
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVVAVDGPS GTGKSSVAKE LARQLGASYL DTGAMYRIVT LWVLRAGVDL TDPAAIAAAT DQVPMSVSSD PDAQTALLAG EDVSVPIRGN EVTGAVSAVS AVPAVRERLV RQQRELAESS GAVVVEGRDI GTVVLPDADV KIYLTASAQA RAQRRNAQNV SGGGDDEYEK VLADVQRRDH LDSTRAVSPL RPAEDALEVD TSDMTQEQVV AHLLDLVRTR AGASR
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtggttgcc gtagatggcc catccggtac cggaaagtcc tccgtcgcaa aagaattggc tcgtcagcta ggggcctcat atctcgatac cggtgcgatg taccggatcg tgacgctctg ggtgctgcgt gccggtgtag acctcaccga ccccgcggcc atcgccgcgg caaccgatca ggtgcccatg tccgtgagtt cggatcccga tgcgcagacg gctctgcttg cgggtgaaga tgtttcggtc ccaatccggg gtaatgaggt gaccggcgcg gtctccgccg tgtccgcggt accggccgtc cgtgaacgac tggtgcggca gcagcgcgag ctggccgagt ccagtggcgc cgtggtggtg gagggccgtg atatcggcac cgtggtgctg ccggacgccg acgtgaagat ctatctgacc gcatcggcac aggcgcgggc gcagcgccgc aacgcgcaga acgtctccgg tggtggcgac gacgaatatg aaaaggtgct tgccgatgtg cagcgccgtg accatctgga ttccacccgg gccgtctcgc cgctgcgccc ggccgaggat gctttagagg tcgataccag cgatatgaca caggaacagg tcgtggccca cctgctggac ctggtgcgca ctagggcagg ggcatcacga taaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa