MypaA.00663.a

Cytidylate kinase

CENTER ID: MypaA.00663.a
ORGANISM: Mycobacterium paratuberculosis ATCC BAA-968 / K-10
ASSOCIATED DISEASE: Johne's disease
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MypaA.00663.a.A1.GE29475 Full length( MypaA.00663.a ) 1 228
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MypaA.00663.a.A1.PS00752 Full length( MypaA.00663.a ) 1 228
MypaA.00663.a.A1.PS00753 Full length( MypaA.00663.a ) 1 228

External Resources

RESOURCE REFERENCE ID
OrthoMCL: OG5_133121
PATRIC ID: fig|262316.17.peg.1488
RefSeq: NP_960348.1
UniProt: Q740D7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSSDVVVAID GPAGTGKSSV SKGLARALGA RYLDTGGMYR MVTLAVLRAG IDPADAEAVG RAAQAVRMSV DYHPDGDRYF LGGEDVSTEI RGDKVTAAVS AVSSVPAVRT RLVGLQREMA SGPGSIVVEG RDIGTVVLPD APVKIFLTAS AETRARRRND QNVAAGLADD YEAVLADVRR RDHLDSTRRV SPLRAAPDAV VVDTSDMTEA QVIDHLRDLV RQRSEVAR
NT Sequence
GTGAGCTCCG ACGTCGTGGT GGCCATCGAT GGCCCCGCTG GGACCGGAAA GTCCTCCGTC TCAAAGGGAT TGGCGCGCGC GCTGGGTGCG CGCTATCTGG ACACCGGGGG AATGTACCGG ATGGTGACGC TGGCGGTGTT GCGCGCCGGC ATCGACCCGG CCGACGCCGA AGCCGTCGGG CGCGCCGCGC AGGCGGTGCG GATGTCGGTG GACTATCACC CCGACGGTGA CCGTTATTTC CTTGGCGGAG AAGACGTTTC CACCGAGATC CGGGGCGACA AGGTCACCGC GGCGGTGTCC GCGGTGTCGT CGGTTCCCGC CGTGCGCACC CGCCTGGTCG GCTTGCAGCG TGAAATGGCT TCCGGCCCAG GCAGTATCGT CGTCGAGGGC CGAGACATCG GCACCGTGGT CCTGCCCGAC GCGCCGGTGA AGATCTTCCT GACCGCCTCG GCCGAGACCC GGGCCCGGCG GCGCAACGAC CAGAACGTGG CGGCGGGGCT GGCCGACGAC TACGAGGCGG TGCTCGCCGA CGTCCGCCGG CGCGACCACC TGGATTCCAC CCGGCGGGTG TCGCCGTTGC GGGCGGCTCC CGACGCGGTG GTGGTCGACA CCAGTGACAT GACCGAGGCG CAGGTGATCG ACCACCTGCG GGACCTGGTC AGACAGCGAA GCGAAGTGGC GCGG
Details for MypaA.00663.a.A1.GE29475
HARVESTED ON: 5/27/2010
SEQUENCED ON: 6/14/2010
EXPECTED MW: 26kDa
OBSERVED MW: 27kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVSSDVVVA IDGPAGTGKS SVSKGLARAL GARYLDTGGM YRMVTLAVLR AGIDPADAEA VGRAAQAVRM SVDYHPDGDR YFLGGEDVST EIRGDKVTAA VSAVSSVPAV RTRLVGLQRE MASGPGSIVV EGRDIGTVVL PDAPVKIFLT ASAETRARRR NDQNVAAGLA DDYEAVLADV RRRDHLDSTR RVSPLRAAPD AVVVDTSDMT EAQVIDHLRD LVRQRSEVAR
Validated NT Sequence
ttattaccgc gccacttcgc ttcgctgtct gaccaggtcc cgcaggtggt cgatcacctg cgcctcggtc atgtcactgg tgtcgaccac caccgcgtcg ggagccgccc gcaacggcga cacccgccgg gtggaatcca ggtggtcgcg ccggcggacg tcggcgagca ccgcctcgta gtcgtcggcc agccccgccg ccacgttctg gtcgttgcgc cgccgggccc gggtctcggc cgaggcggtc aggaagatct tcaccggcgc gtcgggcagg accacggtgc cgatgtctcg gccctcgacg acgatactgc ctgggccgga agccatttca cgctgcaagc cgaccaggcg ggtgcgcacg gcgggaaccg acgacaccgc ggacaccgcc gcggtgacct tgtcgccccg gatctcggtg gaaacgtctt ctccgccaag gaaataacgg tcaccgtcgg ggtgatagtc caccgacatc cgcaccgcct gcgcggcgcg cccgacggct tcggcgtcgg ccgggtcgat gccggcgcgc aacaccgcca gcgtcaccat ccggtacatt cccccggtgt ccagatagcg cgcacccagc gcgcgcgcca atccctttga gacggaggac tttccggtcc cagcggggcc atcgatggcc accacgacgt cggagctcac catcgaacca ggaccctggg tctgagcttc cagggtaccc atatgatggt gatggtgatg agccat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVSSDVVVA IDGPAGTGKS SVSKGLARAL GARYLDTGGM YRMVTLAVLR AGIDPADAEA VGRAAQAVRM SVDYHPDGDR YFLGGEDVST EIRGDKVTAA VSAVSSVPAV RTRLVGLQRE MASGPGSIVV EGRDIGTVVL PDAPVKIFLT ASAETRARRR NDQNVAAGLA DDYEAVLADV RRRDHLDSTR RVSPLRAAPD AVVVDTSDMT EAQVIDHLRD LVRQRSEVAR
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgagctcc gacgtcgtgg tggccatcga tggccccgct gggaccggaa agtcctccgt ctcaaaggga ttggcgcgcg cgctgggtgc gcgctatctg gacaccgggg gaatgtaccg gatggtgacg ctggcggtgt tgcgcgccgg catcgacccg gccgacgccg aagccgtcgg gcgcgccgcg caggcggtgc ggatgtcggt ggactatcac cccgacggtg accgttattt ccttggcgga gaagacgttt ccaccgagat ccggggcgac aaggtcaccg cggcggtgtc cgcggtgtcg tcggttcccg ccgtgcgcac ccgcctggtc ggcttgcagc gtgaaatggc ttccggccca ggcagtatcg tcgtcgaggg ccgagacatc ggcaccgtgg tcctgcccga cgcgccggtg aagatcttcc tgaccgcctc ggccgagacc cgggcccggc ggcgcaacga ccagaacgtg gcggcggggc tggccgacga ctacgaggcg gtgctcgccg acgtccgccg gcgcgaccac ctggattcca cccggcgggt gtcgccgttg cgggcggctc ccgacgcggt ggtggtcgac accagtgaca tgaccgaggc gcaggtgatc gaccacctgc gggacctggt cagacagcga agcgaagtgg cgcggaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa
Details for MypaA.00663.a.A1.PS00752
PURIFICATION DATe: 9/2/2010
CONCENTRATION: 25.11mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 9
VIAL VOLUME: data unavailable
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVSSDVVVA IDGPAGTGKS SVSKGLARAL GARYLDTGGM YRMVTLAVLR AGIDPADAEA VGRAAQAVRM SVDYHPDGDR YFLGGEDVST EIRGDKVTAA VSAVSSVPAV RTRLVGLQRE MASGPGSIVV EGRDIGTVVL PDAPVKIFLT ASAETRARRR NDQNVAAGLA DDYEAVLADV RRRDHLDSTR RVSPLRAAPD AVVVDTSDMT EAQVIDHLRD LVRQRSEVAR
Validated NT Sequence
ttattaccgc gccacttcgc ttcgctgtct gaccaggtcc cgcaggtggt cgatcacctg cgcctcggtc atgtcactgg tgtcgaccac caccgcgtcg ggagccgccc gcaacggcga cacccgccgg gtggaatcca ggtggtcgcg ccggcggacg tcggcgagca ccgcctcgta gtcgtcggcc agccccgccg ccacgttctg gtcgttgcgc cgccgggccc gggtctcggc cgaggcggtc aggaagatct tcaccggcgc gtcgggcagg accacggtgc cgatgtctcg gccctcgacg acgatactgc ctgggccgga agccatttca cgctgcaagc cgaccaggcg ggtgcgcacg gcgggaaccg acgacaccgc ggacaccgcc gcggtgacct tgtcgccccg gatctcggtg gaaacgtctt ctccgccaag gaaataacgg tcaccgtcgg ggtgatagtc caccgacatc cgcaccgcct gcgcggcgcg cccgacggct tcggcgtcgg ccgggtcgat gccggcgcgc aacaccgcca gcgtcaccat ccggtacatt cccccggtgt ccagatagcg cgcacccagc gcgcgcgcca atccctttga gacggaggac tttccggtcc cagcggggcc atcgatggcc accacgacgt cggagctcac catcgaacca ggaccctggg tctgagcttc cagggtaccc atatgatggt gatggtgatg agccat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVSSDVVVA IDGPAGTGKS SVSKGLARAL GARYLDTGGM YRMVTLAVLR AGIDPADAEA VGRAAQAVRM SVDYHPDGDR YFLGGEDVST EIRGDKVTAA VSAVSSVPAV RTRLVGLQRE MASGPGSIVV EGRDIGTVVL PDAPVKIFLT ASAETRARRR NDQNVAAGLA DDYEAVLADV RRRDHLDSTR RVSPLRAAPD AVVVDTSDMT EAQVIDHLRD LVRQRSEVAR
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGAGCTCC GACGTCGTGG TGGCCATCGA TGGCCCCGCT GGGACCGGAA AGTCCTCCGT CTCAAAGGGA TTGGCGCGCG CGCTGGGTGC GCGCTATCTG GACACCGGGG GAATGTACCG GATGGTGACG CTGGCGGTGT TGCGCGCCGG CATCGACCCG GCCGACGCCG AAGCCGTCGG GCGCGCCGCG CAGGCGGTGC GGATGTCGGT GGACTATCAC CCCGACGGTG ACCGTTATTT CCTTGGCGGA GAAGACGTTT CCACCGAGAT CCGGGGCGAC AAGGTCACCG CGGCGGTGTC CGCGGTGTCG TCGGTTCCCG CCGTGCGCAC CCGCCTGGTC GGCTTGCAGC GTGAAATGGC TTCCGGCCCA GGCAGTATCG TCGTCGAGGG CCGAGACATC GGCACCGTGG TCCTGCCCGA CGCGCCGGTG AAGATCTTCC TGACCGCCTC GGCCGAGACC CGGGCCCGGC GGCGCAACGA CCAGAACGTG GCGGCGGGGC TGGCCGACGA CTACGAGGCG GTGCTCGCCG ACGTCCGCCG GCGCGACCAC CTGGATTCCA CCCGGCGGGT GTCGCCGTTG CGGGCGGCTC CCGACGCGGT GGTGGTCGAC ACCAGTGACA TGACCGAGGC GCAGGTGATC GACCACCTGC GGGACCTGGT CAGACAGCGA AGCGAAGTGG CGCGGAAACA GCACGAACAA GTTCTGCAGC CAAGCTTCTC GAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAA
Details for MypaA.00663.a.A1.PS00753
PURIFICATION DATe: 9/2/2010
CONCENTRATION: 55.08mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 8
VIAL VOLUME: data unavailable
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVSSDVVVA IDGPAGTGKS SVSKGLARAL GARYLDTGGM YRMVTLAVLR AGIDPADAEA VGRAAQAVRM SVDYHPDGDR YFLGGEDVST EIRGDKVTAA VSAVSSVPAV RTRLVGLQRE MASGPGSIVV EGRDIGTVVL PDAPVKIFLT ASAETRARRR NDQNVAAGLA DDYEAVLADV RRRDHLDSTR RVSPLRAAPD AVVVDTSDMT EAQVIDHLRD LVRQRSEVAR
Validated NT Sequence
ttattaccgc gccacttcgc ttcgctgtct gaccaggtcc cgcaggtggt cgatcacctg cgcctcggtc atgtcactgg tgtcgaccac caccgcgtcg ggagccgccc gcaacggcga cacccgccgg gtggaatcca ggtggtcgcg ccggcggacg tcggcgagca ccgcctcgta gtcgtcggcc agccccgccg ccacgttctg gtcgttgcgc cgccgggccc gggtctcggc cgaggcggtc aggaagatct tcaccggcgc gtcgggcagg accacggtgc cgatgtctcg gccctcgacg acgatactgc ctgggccgga agccatttca cgctgcaagc cgaccaggcg ggtgcgcacg gcgggaaccg acgacaccgc ggacaccgcc gcggtgacct tgtcgccccg gatctcggtg gaaacgtctt ctccgccaag gaaataacgg tcaccgtcgg ggtgatagtc caccgacatc cgcaccgcct gcgcggcgcg cccgacggct tcggcgtcgg ccgggtcgat gccggcgcgc aacaccgcca gcgtcaccat ccggtacatt cccccggtgt ccagatagcg cgcacccagc gcgcgcgcca atccctttga gacggaggac tttccggtcc cagcggggcc atcgatggcc accacgacgt cggagctcac catcgaacca ggaccctggg tctgagcttc cagggtaccc atatgatggt gatggtgatg agccat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVSSDVVVA IDGPAGTGKS SVSKGLARAL GARYLDTGGM YRMVTLAVLR AGIDPADAEA VGRAAQAVRM SVDYHPDGDR YFLGGEDVST EIRGDKVTAA VSAVSSVPAV RTRLVGLQRE MASGPGSIVV EGRDIGTVVL PDAPVKIFLT ASAETRARRR NDQNVAAGLA DDYEAVLADV RRRDHLDSTR RVSPLRAAPD AVVVDTSDMT EAQVIDHLRD LVRQRSEVAR
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGAGCTCC GACGTCGTGG TGGCCATCGA TGGCCCCGCT GGGACCGGAA AGTCCTCCGT CTCAAAGGGA TTGGCGCGCG CGCTGGGTGC GCGCTATCTG GACACCGGGG GAATGTACCG GATGGTGACG CTGGCGGTGT TGCGCGCCGG CATCGACCCG GCCGACGCCG AAGCCGTCGG GCGCGCCGCG CAGGCGGTGC GGATGTCGGT GGACTATCAC CCCGACGGTG ACCGTTATTT CCTTGGCGGA GAAGACGTTT CCACCGAGAT CCGGGGCGAC AAGGTCACCG CGGCGGTGTC CGCGGTGTCG TCGGTTCCCG CCGTGCGCAC CCGCCTGGTC GGCTTGCAGC GTGAAATGGC TTCCGGCCCA GGCAGTATCG TCGTCGAGGG CCGAGACATC GGCACCGTGG TCCTGCCCGA CGCGCCGGTG AAGATCTTCC TGACCGCCTC GGCCGAGACC CGGGCCCGGC GGCGCAACGA CCAGAACGTG GCGGCGGGGC TGGCCGACGA CTACGAGGCG GTGCTCGCCG ACGTCCGCCG GCGCGACCAC CTGGATTCCA CCCGGCGGGT GTCGCCGTTG CGGGCGGCTC CCGACGCGGT GGTGGTCGAC ACCAGTGACA TGACCGAGGC GCAGGTGATC GACCACCTGC GGGACCTGGT CAGACAGCGA AGCGAAGTGG CGCGGAAACA GCACGAACAA GTTCTGCAGC CAAGCTTCTC GAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAA