MytuD.00587.a

MYCOBACTERIAL PERSISTENCE REGULATOR MRPA (TWO COMPONENT RESPONSE TRANSCRIPTIONAL REGULATORY PROTEIN)

CENTER ID:	MytuD.00587.a
ORGANISM:	Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE:	Tuberculosis
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MytuD.00587.a.A1.PW25917	Full length( MytuD.00587.a )		1	230

External Resources

RESOURCE	REFERENCE ID
OrthoMCL:	OG5_128013
PATRIC ID:	fig\|83332.12.peg.1094
RefSeq:	NP_215496.1
TubercuList:	Rv0981
MTB Network Portal:	Rv0981
UniProt:	P9WGM9

Orthologues for Rv0981

Species	% Identity	% Coverage	Clones	Proteins
avium	97	99	1	1
marinum	95	100	1	1
ulcerans	94	100	1	0
leprae	94	99	1	1
smegmatis	90	100	0	0
thermoresistibile	89	80	1	1
abscessus	88	100	1	1
abscessus	43	98	1	1
avium	42	97	0	0
paratuberculosis	41	97	0	0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSVRILVVDD DRAVRESLRR SLSFNGYSVE LAHDGVEALD MIASDRPDAL VLDVMMPRLD GLEVCRQLRG TGDDLPILVL TARDSVSERV AGLDAGADDY LPKPFALEEL LARMRALLRR TKPEDAAESM AMRFSDLTLD PVTREVNRGQ RRISLTRTEF ALLEMLIANP RRVLTRSRIL EEVWGFDFPT SGNALEVYVG YLRRKTEADG EPRLIHTVRG VGYVLRETPP

NT Sequence

GTGTCCGTGC GAATTCTTGT CGTTGACGAC GATCGTGCGG TGCGCGAGTC GCTGCGCCGG TCGCTTTCCT TCAATGGCTA TTCGGTCGAA CTGGCCCACG ACGGGGTTGA GGCGCTCGAC ATGATTGCCA GCGATCGCCC CGACGCGTTG GTCCTGGATG TCATGATGCC GCGGCTGGAC GGCCTCGAGG TGTGCCGTCA GCTCCGCGGC ACCGGCGACG ACCTGCCGAT TCTGGTGCTG ACCGCGCGCG ACTCGGTGTC CGAGCGGGTG GCCGGGCTGG ACGCCGGTGC CGACGACTAC CTACCAAAGC CGTTCGCCCT CGAAGAGCTG CTGGCACGGA TGCGGGCGCT GCTGCGCCGC ACCAAGCCCG AGGATGCCGC CGAGTCGATG GCCATGAGGT TCTCCGACCT GACGCTGGAC CCGGTAACCC GCGAAGTCAA CCGTGGACAG CGCCGGATCA GCCTGACCCG CACCGAATTT GCATTGCTGG AGATGCTGAT CGCCAATCCG CGGCGAGTGC TGACGCGCAG CCGTATCCTG GAAGAGGTAT GGGGATTCGA CTTTCCCACC TCGGGCAACG CGCTGGAAGT CTACGTCGGG TATCTACGCC GCAAGACCGA GGCCGACGGC GAGCCGCGGC TGATCCACAC TGTGCGCGGA GTGGGTTACG TGCTACGTGA AACACCACCC

Details for MytuD.00587.a.A1.PW25917

PURIFICATION DATe:	3/31/2009
CONCENTRATION:	1.94mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	31
VIAL VOLUME:	200µl
PERCENT IDENTITY:	99
PERCENT COVERAGE:	95

Protocol Notes

notes unavailable

Validated AA Sequence

MXXLEAQTQG PGSMVSVRIL VVDDDRAVRE SLRRSLSFNG YSVELAHDGV EALDMIASDR PDALVLDVMM PRLDGLEVCR QLRGTGDDLP ILVLTARDSV SERVAGLDAG ADDYLPKPFA LEELLARMRA LLRRTKPEDA AESMAMRFSD LTLDPVTREV NRGQRRISLT RTEFALLEML IANPRRVLTR SRILEEVWGF DFPTSGNALE VYVGYLRRKT EADGEPRLIH TVRGVGYVLR E

Validated NT Sequence

tttcacgtag cacgtaaccc actccgcgca cagtgtggat cagccgcggc tcgccgtcgg cctcggtctt gcggcgtaga tacccgacgt agacttccag cgcgttgccc gaggtgggaa agtcgaatcc ccatacctct tccaggatac ggctgcgcgt cagcactcgc cgcggattgg cgatcagcat ctccagcaat gcaaattcgg tgcgggtcag gctgatccgg cgctgtccac ggttgacttc gcgggttacc gggtccagcg tcaggtcgga gaacctcatg gccatcgact cggcggcatc ctcgggcttg gtgcggcgca gcagcgcccg catccgtgcc agcagctctt cgagggcgaa cggctttggt aggtagtcgt cggcaccggc gtccagcccg gccacccgct cggacaccga gtcgcgcgcg gtcagcacca gaatcggcag gtcgtcgccg gtgccgcgga gctgacggca cacctcgagg ccgtccagcc gcggcatcat gacatccagg accaacgcgt cggggcgatc gctggcaatc atgtcgagcg cctcaacccc gtcgtgggcc agttcgaccg aatagccatt gaaggaaagc gaccggcgca gcgactcgcg caccgcacga tcgtcgtcaa cgacaagaat tcgcacggac accatcgaac caggaccctg ggtctgagct tccagggnnn ccatatgatg gtganggtga tnagccanng t

Expressed Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMVSVRILVV DDDRAVRESL RRSLSFNGYS VELAHDGVEA LDMIASDRPD ALVLDVMMPR LDGLEVCRQL RGTGDDLPIL VLTARDSVSE RVAGLDAGAD DYLPKPFALE ELLARMRALL RRTKPEDAAE SMAMRFSDLT LDPVTREVNR GQRRISLTRT EFALLEMLIA NPRRVLTRSR ILEEVWGFDF PTSGNALEVY VGYLRRKTEA DGEPRLIHTV RGVGYVLRET PP

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGTCCGTG CGAATTCTTG TCGTTGACGA CGATCGTGCG GTGCGCGAGT CGCTGCGCCG GTCGCTTTCC TTCAATGGCT ATTCGGTCGA ACTGGCCCAC GACGGGGTTG AGGCGCTCGA CATGATTGCC AGCGATCGCC CCGACGCGTT GGTCCTGGAT GTCATGATGC CGCGGCTGGA CGGCCTCGAG GTGTGCCGTC AGCTCCGCGG CACCGGCGAC GACCTGCCGA TTCTGGTGCT GACCGCGCGC GACTCGGTGT CCGAGCGGGT GGCCGGGCTG GACGCCGGTG CCGACGACTA CCTACCAAAG CCGTTCGCCC TCGAAGAGCT GCTGGCACGG ATGCGGGCGC TGCTGCGCCG CACCAAGCCC GAGGATGCCG CCGAGTCGAT GGCCATGAGG TTCTCCGACC TGACGCTGGA CCCGGTAACC CGCGAAGTCA ACCGTGGACA GCGCCGGATC AGCCTGACCC GCACCGAATT TGCATTGCTG GAGATGCTGA TCGCCAATCC GCGGCGAGTG CTGACGCGCA GCCGTATCCT GGAAGAGGTA TGGGGATTCG ACTTTCCCAC CTCGGGCAAC GCGCTGGAAG TCTACGTCGG GTATCTACGC CGCAAGACCG AGGCCGACGG CGAGCCGCGG CTGATCCACA CTGTGCGCGG AGTGGGTTAC GTGCTACGTG AAACACCACC CAAACAGCAC GAACAAGTTC TGCAGCCAAG CTTCTCGAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAA