SSGCID: Target Page

MyulA.00587.b

Response regulator mprA

CENTER ID:	MyulA.00587.b
ORGANISM:	Mycobacterium ulcerans Agy99
ASSOCIATED DISEASE:	Buruli, Bairnsdale ulcer
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyulA.00587.b.A1.GE29710	Full length( MyulA.00587.b )		1	232

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|362242.7.peg.5428
RefSeq:	YP_908104.1
UniProt:	A0PWB4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSVRILVVDD DRAVRESLRR SLSFNGYTVE LAHDGVEALE MIASDRPDAL VLDVMMPRLD GLEVCRQLRS TGDDLPILVL TARDSVSERV AGLDAGADDY LPKPFALEEL LARMRALLRR TKLEDDEAAD SVAMTFSDLS LDPVTREVTR GARRISLTRT EFALLEMLIA NPRRVLTRSR ILEEVWGFDF PTSGNALEVY VGYLRRKTEA EGEPRLIHTV RGVGYVLRET PP

NT Sequence

ATGTCCGTGC GGATACTTGT CGTCGACGAC GATCGCGCGG TGCGCGAGTC GCTGCGCAGG TCGCTTTCCT TCAATGGCTA CACGGTTGAG CTGGCACACG ATGGTGTGGA AGCTCTCGAG ATGATTGCCA GTGATCGGCC TGACGCGTTG GTGCTGGACG TCATGATGCC GCGACTGGAT GGCTTGGAGG TATGCCGTCA ACTTCGCAGC ACCGGTGATG ATCTGCCCAT TCTCGTGCTG ACCGCCCGGG ATTCCGTCTC CGAACGGGTC GCGGGGCTCG ATGCCGGCGC GGATGACTAC CTGCCGAAGC CGTTCGCGCT GGAAGAGCTG TTGGCGCGGA TGCGCGCGTT GCTGCGCCGC ACCAAGCTCG AAGACGACGA AGCCGCCGAT TCGGTAGCCA TGACGTTCTC CGACCTGAGC CTGGACCCGG TGACCCGGGA GGTGACCCGC GGGGCGCGGC GCATCAGCCT CACCCGAACC GAGTTCGCCC TGCTGGAGAT GCTGATCGCC AACCCGCGAC GGGTGCTGAC CCGCAGCCGC ATCCTCGAAG AGGTGTGGGG CTTCGACTTT CCCACGTCCG GCAACGCGCT CGAGGTCTAC GTGGGCTACC TGCGGCGTAA AACCGAGGCC GAGGGCGAGC CGCGGCTGAT CCACACCGTG CGAGGGGTGG GTTACGTGCT ACGCGAAACG CCACCC

Details for MyulA.00587.b.A1.GE29710

HARVESTED ON:	6/29/2010
SEQUENCED ON:	7/7/2010
EXPECTED MW:	28kDa
OBSERVED MW:	31kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMSVRILVVD DDRAVRESLR RSLSFNGYTV ELAHDGVEAL EMIASDRPDA LVLDVMMPRL DGLEVCRQLR STGDDLPILV LTARDSVSER VAGLDAGADD YLPKPFALEE LLARMRALLR RTKLEDDEAA DSVAMTFSDL SLDPVTREVT RGARRISLTR TEFALLEMLI ANPRRVLTRS RILEEVWGFD FPTSGNALEV YVGYLRRKTE AEGEPRLIHT VRGVGYVLRE TPP

Validated NT Sequence

ttttgtttac tttaagaagg aganatacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatgtccgt gcggatactt gtcgtcgacg acgatcgcgc ggtgcgcgag tcgctgcgca ggtcgctttc cttcaatggc tacacggttg agctggcaca cgatggtgtg gaagctctcg agatgattgc cagtgatcgg cctgacgcgt tggtgctgga cgtcatgatg ccgcgactgg atggcttgga ggtatgccgt caacttcgca gcaccggtga tgatctgccc attctcgtgc tgaccgcccg ggattccgtc tccgaacggg tcgcggggct cgatgccggc gcggatgact acctgccgaa gccgttcgcg ctggaagagc tgttggcgcg gatgcgcgcg ttgctgcgcc gcaccaagct cgaagacgac gaagccgccg attcggtagc catgacgttc tccgacctga gcctggaccc ggtgacccgg gaggtgaccc gcggggcgcg gcgcatcagc ctcacccgaa ccgagttcgc cctgctggag atgctgatcg ccaacccgcg acgggtgctg acccgcagcc gcatcctcga agaggtgtgg ggcttcgact ttcccacgtc cggcaacgcg ctcgaggtct acgtgggcta cctgcggcgt aaaaccgagg ccgagggcga gccgcggctg atccacaccg tgcgaggggt gggttacgtg ctacgcgaaa cgccacccta ataaacagca cgaacaagtt ctgcagccna gcttctcgag gatccggctg ctaacaaagc ccgaaaggna gctgagttgg ctgctgccac cgctgancan nnnnannnnn na

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtccgtgcgg atacttgtcg tcgacgacga tcgcgcggtg cgcgagtcgc tgcgcaggtc gctttccttc aatggctaca cggttgagct ggcacacgat ggtgtggaag ctctcgagat gattgccagt gatcggcctg acgcgttggt gctggacgtc atgatgccgc gactggatgg cttggaggta tgccgtcaac ttcgcagcac cggtgatgat ctgcccattc tcgtgctgac cgcccgggat tccgtctccg aacgggtcgc ggggctcgat gccggcgcgg atgactacct gccgaagccg ttcgcgctgg aagagctgtt ggcgcggatg cgcgcgttgc tgcgccgcac caagctcgaa gacgacgaag ccgccgattc ggtagccatg acgttctccg acctgagcct ggacccggtg acccgggagg tgacccgcgg ggcgcggcgc atcagcctca cccgaaccga gttcgccctg ctggagatgc tgatcgccaa cccgcgacgg gtgctgaccc gcagccgcat cctcgaagag gtgtggggct tcgactttcc cacgtccggc aacgcgctcg aggtctacgt gggctacctg cggcgtaaaa ccgaggccga gggcgagccg cggctgatcc acaccgtgcg aggggtgggt tacgtgctac gcgaaacgcc acccaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa