MyavA.00587.a

Response regulator mprA

CENTER ID: MyavA.00587.a
ORGANISM: Mycobacterium avium 104
ASSOCIATED DISEASE: Johne's disease, paratuberculosis
CURRENT STATUS: crystallized
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyavA.00587.a.A1.GE28556 Full length( MyavA.00587.a ) 1 228
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyavA.00587.a.A1.PS00610 Full length( MyavA.00587.a ) 1 228

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|243243.7.peg.1067
RefSeq: YP_880347.1
UniProt: A0QBQ9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRILVVDDDR AVRESLRRSL SFNGYSVELA HDGVEALDMI ASDRPDALVL DVMMPRLDGL EVCRQLRSTG DDLPILVLTA RDSVSERVAG LDAGADDYLP KPFALEELLA RMRALLRRTK PEDDAESVAM TFSDLTLDPV TREVTRGQRR ISLTRTEFAL LEMLIANPRR VLTRSRILEE VWGFDFPTSG NALEVYVGYL RRKTEADGEP RLIHTVRGVG YVLRETPP
NT Sequence
GTGCGAATAC TGGTCGTCGA CGACGATCGC GCGGTGCGCG AGTCGTTACG CAGGTCGTTG TCCTTCAACG GCTACTCCGT CGAGTTGGCC CATGACGGGG TCGAGGCTCT CGACATGATC GCGAGCGATC GCCCCGACGC GCTCGTCCTG GATGTGATGA TGCCGCGACT GGATGGCCTC GAGGTGTGCC GTCAGCTGCG CAGCACCGGT GACGACCTGC CCATCCTGGT GCTGACCGCC CGCGATTCGG TCTCCGAGCG GGTGGCCGGG CTGGACGCCG GCGCCGACGA CTACCTGCCC AAGCCGTTCG CCCTGGAAGA ACTGCTGGCC CGGATGCGGG CGCTGCTGCG CCGCACCAAG CCCGAGGACG ACGCCGAATC CGTGGCGATG ACGTTCTCCG ATCTGACGCT GGACCCGGTG ACCCGGGAGG TCACCCGCGG GCAGCGCCGG ATCAGCCTGA CCCGCACCGA GTTCGCACTG CTGGAAATGC TGATCGCCAA CCCGCGCCGG GTGCTCACCC GCAGCCGCAT CCTCGAGGAG GTCTGGGGCT TCGACTTCCC CACCTCGGGC AATGCGCTGG AGGTGTACGT CGGCTACCTG CGCCGCAAAA CCGAAGCCGA CGGCGAGCCT CGGTTGATCC ACACGGTGCG CGGGGTGGGT TACGTGCTTC GGGAGACGCC GCCG
Details for MyavA.00587.a.A1.GE28556
HARVESTED ON: 2/9/2010
SEQUENCED ON: 2/22/2010
EXPECTED MW: 28kDa
OBSERVED MW: 28kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 96
Validated AA Sequence
MGTLEAQTQG PGSMVRILVV DDDRAVRESL RRSLSFNGYS VELAHDGVEA LDMIASDRPD ALVLDVMMPR LDGLEVCRQL RSTGDDLPIL VLTARDSVSE RVAGLDAGAD DYLPKPFALE ELLARMRALL RRTKPEDDAE SVAMTFSDLT LDPVTREVTR GQRRISLTRT EFALLEMLIA NPRRVLTRSR ILEEVWGFDF PTSGNALEVY VGYLRRKTEA DGEPRLIHTV RGVGYVLRET PP
Validated NT Sequence
tcaccatcat atgggtaccc tggaagctca gacccagggt cctggttcga tggtgcgaat actggtcgtc gacgacgatc gcgcggtgcg cgagtcgtta cgcaggtcgt tgtccttcaa cggctactcc gtcgagttgg cccatgacgg ggtcgaggct ctcgacatga tcgcgagcga tcgccccgac gcgctcgtcc tggatgtgat gatgccgcga ctggatggcc tcgaggtgtg ccgtcagctg cgcagcaccg gtgacgacct gcccatcctg gtgctgaccg cccgcgattc ggtctccgag cgggtggccg ggctggacgc cggcgccgac gactacctgc ccaagccgtt cgccctggaa gaactgctgg cccggatgcg ggcgctgctg cgccgcacca agcccgagga cgacgccgaa tccgtggcga tgacgttctc cgatctgacg ctggacccgg tgacccggga ggtcacccgc gggcagcgcc ggatcagcct gacccgcacc gagttcgcac tgctggaaat gctgatcgcc aacccgcgcc gggtgctcac ccgcagccgc atcctcgagg aggtctgggg cttcgacttc cccacctcgg gcaatgcgct ggaggtgtac gtcggctacc tgcgccgcaa aaccgaagcc gacggcgagc ctcggttgat ccacacggtg cgcggggtgg gttacgtgct tcgggagacg ccgccgtaat aa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVRILVVDD DRAVRESLRR SLSFNGYSVE LAHDGVEALD MIASDRPDAL VLDVMMPRLD GLEVCRQLRS TGDDLPILVL TARDSVSERV AGLDAGADDY LPKPFALEEL LARMRALLRR TKPEDDAESV AMTFSDLTLD PVTREVTRGQ RRISLTRTEF ALLEMLIANP RRVLTRSRIL EEVWGFDFPT SGNALEVYVG YLRRKTEADG EPRLIHTVRG VGYVLRETPP
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgcgaata ctggtcgtcg acgacgatcg cgcggtgcgc gagtcgttac gcaggtcgtt gtccttcaac ggctactccg tcgagttggc ccatgacggg gtcgaggctc tcgacatgat cgcgagcgat cgccccgacg cgctcgtcct ggatgtgatg atgccgcgac tggatggcct cgaggtgtgc cgtcagctgc gcagcaccgg tgacgacctg cccatcctgg tgctgaccgc ccgcgattcg gtctccgagc gggtggccgg gctggacgcc ggcgccgacg actacctgcc caagccgttc gccctggaag aactgctggc ccggatgcgg gcgctgctgc gccgcaccaa gcccgaggac gacgccgaat ccgtggcgat gacgttctcc gatctgacgc tggacccggt gacccgggag gtcacccgcg ggcagcgccg gatcagcctg acccgcaccg agttcgcact gctggaaatg ctgatcgcca acccgcgccg ggtgctcacc cgcagccgca tcctcgagga ggtctggggc ttcgacttcc ccacctcggg caatgcgctg gaggtgtacg tcggctacct gcgccgcaaa accgaagccg acggcgagcc tcggttgatc cacacggtgc gcggggtggg ttacgtgctt cgggagacgc cgccgaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa
Details for MyavA.00587.a.A1.PS00610
PURIFICATION DATe: 5/24/2010
CONCENTRATION: 61.64mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 4
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 96
Protocol Notes
notes unavailable
Validated AA Sequence
MGTLEAQTQG PGSMVRILVV DDDRAVRESL RRSLSFNGYS VELAHDGVEA LDMIASDRPD ALVLDVMMPR LDGLEVCRQL RSTGDDLPIL VLTARDSVSE RVAGLDAGAD DYLPKPFALE ELLARMRALL RRTKPEDDAE SVAMTFSDLT LDPVTREVTR GQRRISLTRT EFALLEMLIA NPRRVLTRSR ILEEVWGFDF PTSGNALEVY VGYLRRKTEA DGEPRLIHTV RGVGYVLRET PP
Validated NT Sequence
tcaccatcat atgggtaccc tggaagctca gacccagggt cctggttcga tggtgcgaat actggtcgtc gacgacgatc gcgcggtgcg cgagtcgtta cgcaggtcgt tgtccttcaa cggctactcc gtcgagttgg cccatgacgg ggtcgaggct ctcgacatga tcgcgagcga tcgccccgac gcgctcgtcc tggatgtgat gatgccgcga ctggatggcc tcgaggtgtg ccgtcagctg cgcagcaccg gtgacgacct gcccatcctg gtgctgaccg cccgcgattc ggtctccgag cgggtggccg ggctggacgc cggcgccgac gactacctgc ccaagccgtt cgccctggaa gaactgctgg cccggatgcg ggcgctgctg cgccgcacca agcccgagga cgacgccgaa tccgtggcga tgacgttctc cgatctgacg ctggacccgg tgacccggga ggtcacccgc gggcagcgcc ggatcagcct gacccgcacc gagttcgcac tgctggaaat gctgatcgcc aacccgcgcc gggtgctcac ccgcagccgc atcctcgagg aggtctgggg cttcgacttc cccacctcgg gcaatgcgct ggaggtgtac gtcggctacc tgcgccgcaa aaccgaagcc gacggcgagc ctcggttgat ccacacggtg cgcggggtgg gttacgtgct tcgggagacg ccgccgtaat aa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVRILVVDD DRAVRESLRR SLSFNGYSVE LAHDGVEALD MIASDRPDAL VLDVMMPRLD GLEVCRQLRS TGDDLPILVL TARDSVSERV AGLDAGADDY LPKPFALEEL LARMRALLRR TKPEDDAESV AMTFSDLTLD PVTREVTRGQ RRISLTRTEF ALLEMLIANP RRVLTRSRIL EEVWGFDFPT SGNALEVYVG YLRRKTEADG EPRLIHTVRG VGYVLRETPP
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGCGAATA CTGGTCGTCG ACGACGATCG CGCGGTGCGC GAGTCGTTAC GCAGGTCGTT GTCCTTCAAC GGCTACTCCG TCGAGTTGGC CCATGACGGG GTCGAGGCTC TCGACATGAT CGCGAGCGAT CGCCCCGACG CGCTCGTCCT GGATGTGATG ATGCCGCGAC TGGATGGCCT CGAGGTGTGC CGTCAGCTGC GCAGCACCGG TGACGACCTG CCCATCCTGG TGCTGACCGC CCGCGATTCG GTCTCCGAGC GGGTGGCCGG GCTGGACGCC GGCGCCGACG ACTACCTGCC CAAGCCGTTC GCCCTGGAAG AACTGCTGGC CCGGATGCGG GCGCTGCTGC GCCGCACCAA GCCCGAGGAC GACGCCGAAT CCGTGGCGAT GACGTTCTCC GATCTGACGC TGGACCCGGT GACCCGGGAG GTCACCCGCG GGCAGCGCCG GATCAGCCTG ACCCGCACCG AGTTCGCACT GCTGGAAATG CTGATCGCCA ACCCGCGCCG GGTGCTCACC CGCAGCCGCA TCCTCGAGGA GGTCTGGGGC TTCGACTTCC CCACCTCGGG CAATGCGCTG GAGGTGTACG TCGGCTACCT GCGCCGCAAA ACCGAAGCCG ACGGCGAGCC TCGGTTGATC CACACGGTGC GCGGGGTGGG TTACGTGCTT CGGGAGACGC CGCCGAAACA GCACGAACAA GTTCTGCAGC CAAGCTTCTC GAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAA