MyabA.00587.b

Putative two-component system response regulator

CENTER ID: MyabA.00587.b
ORGANISM: Mycobacterium abscessus ATCC 19977 / DSM 44196
ASSOCIATED DISEASE: Skin abscess
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyabA.00587.b.A1.GE30317 Full length( MyabA.00587.b ) 1 227
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyabA.00587.b.A1.PW30194 Full length( MyabA.00587.b ) 1 227

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|36809.5.peg.1767
RefSeq: YP_001702459.1
UniProt: B1MNA4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MPSQRRILVV DDEPTICASI SARLRAEGYA VDTEDNGLDA VRHCRENPPD LMVLDVMLPG LDGLEVCRQV QAHAPIPVLM LTARGTETDM LVGLGVGADD YLPKPFSMRV LVARVAALLR RTHRIAPPST DVRIGQVRID VSERRVYHRD DEVHLTRTEF DLLAYLADNP RVAISRDRLL DQVWGWSAGL PSGRRTVDSH IKALRRKLGQ ELIRTVHGIG YALEAPR
NT Sequence
GTGCCCAGCC AGCGAAGAAT CTTGGTCGTT GACGACGAAC CCACGATCTG TGCGTCCATT TCCGCACGGC TGCGCGCCGA GGGGTATGCG GTAGACACCG AGGACAACGG CCTGGACGCG GTGCGGCATT GCCGCGAAAA CCCGCCGGAC CTGATGGTGC TGGATGTCAT GCTGCCCGGA CTCGACGGGC TGGAGGTGTG CCGCCAGGTG CAGGCCCACG CACCCATCCC GGTGCTGATG CTCACCGCGC GGGGCACCGA AACCGACATG CTGGTGGGCC TGGGGGTGGG GGCCGACGAC TACCTGCCCA AGCCCTTCAG CATGCGGGTG CTGGTGGCCC GGGTGGCCGC GCTGCTGCGC CGCACCCATC GGATCGCGCC GCCGAGCACC GATGTGCGCA TCGGGCAGGT ACGCATCGAC GTGTCCGAGC GCCGCGTATA TCACCGCGAC GACGAGGTGC ACCTGACCCG CACCGAATTC GACCTACTGG CCTACCTGGC CGACAACCCG CGGGTGGCCA TCAGTCGTGA TCGTCTGCTC GATCAGGTGT GGGGCTGGAG CGCGGGGCTG CCCTCGGGGC GGCGCACCGT GGACAGTCAC ATCAAGGCGC TGCGCCGCAA GCTCGGTCAG GAGCTGATCC GCACCGTCCA CGGCATCGGA TACGCGTTGG AGGCACCGCG A
Details for MyabA.00587.b.A1.GE30317
HARVESTED ON: 9/8/2010
SEQUENCED ON: 9/17/2010
EXPECTED MW: 27kDa
OBSERVED MW: 27kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVPSQRRIL VVDDEPTICA SISARLRAEG YAVDTEDNGL DAVRHCRENP PDLMVLDVML PGLDGLEVCR QVQAHAPIPV LMLTARGTET DMLVGLGVGA DDYLPKPFSM RVLVARVAAL LRRTHRIAPP STDVRIGQVR IDVSERRVYH RDDEVHLTRT EFDLLAYLAD NPRVAISRDR LLDQVWGWSA GLPSGRRTVD SHIKALRRKL GQELIRTVHG IGYALEAPR
Validated NT Sequence
ttattatcgc ggtgcctcca acgcgtatcc gatgccgtgg acggtgcgga tcagctcctg accgagcttg cggcgcagcg ccttgatgtg actgtccacg gtgcgccgcc ccgagggcag ccccgcgctc cagccccaca cctgatcgag cagacgatca cgactgatgg ccacccgcgg gttgtcggcc aggtaggcca gtaggtcgaa ttcggtgcgg gtcaggtgca cctcgtcgtc gcggtgatat acgcggcgct cggacacgtc gatgcgtacc tgcccgatgc gcacatcggt gctcggcggc gcgatccgat gggtgcggcg cagcagcgcg gccacccggg ccaccagcac ccgcatgctg aagggcttgg gcaggtagtc gtcggccccc acccccaggc ccaccagcat gtcggtttcg gtgccccgcg cggtgagcat cagcaccggg atgggtgcgt gggcctgcac ctggcggcac acctccagcc cgtcgagtcc gggcagcatg acatccagca ccatcaggtc cggcgggttt tcgcggcaat gccgcaccgc gtccaggccg ttgtcctcgg tgtctaccgc atacccctcg gcgcgcagcc gtgcggaaat ggacgcacag atcgtgggtt cgtcgtcaac gaccaagatt cttcgctggc tgggcaccat cgaaccagga ccctgggtct gagcttccag ggtacccata tgatggtgat ggtgatgagc cat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVPSQRRIL VVDDEPTICA SISARLRAEG YAVDTEDNGL DAVRHCRENP PDLMVLDVML PGLDGLEVCR QVQAHAPIPV LMLTARGTET DMLVGLGVGA DDYLPKPFSM RVLVARVAAL LRRTHRIAPP STDVRIGQVR IDVSERRVYH RDDEVHLTRT EFDLLAYLAD NPRVAISRDR LLDQVWGWSA GLPSGRRTVD SHIKALRRKL GQELIRTVHG IGYALEAPR
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgcccagc cagcgaagaa tcttggtcgt tgacgacgaa cccacgatct gtgcgtccat ttccgcacgg ctgcgcgccg aggggtatgc ggtagacacc gaggacaacg gcctggacgc ggtgcggcat tgccgcgaaa acccgccgga cctgatggtg ctggatgtca tgctgcccgg actcgacggg ctggaggtgt gccgccaggt gcaggcccac gcacccatcc cggtgctgat gctcaccgcg cggggcaccg aaaccgacat gctggtgggc ctgggggtgg gggccgacga ctacctgccc aagcccttca gcatgcgggt gctggtggcc cgggtggccg cgctgctgcg ccgcacccat cggatcgcgc cgccgagcac cgatgtgcgc atcgggcagg tacgcatcga cgtgtccgag cgccgcgtat atcaccgcga cgacgaggtg cacctgaccc gcaccgaatt cgacctactg gcctacctgg ccgacaaccc gcgggtggcc atcagtcgtg atcgtctgct cgatcaggtg tggggctgga gcgcggggct gccctcgggg cggcgcaccg tggacagtca catcaaggcg ctgcgccgca agctcggtca ggagctgatc cgcaccgtcc acggcatcgg atacgcgttg gaggcaccgc gataaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa
Details for MyabA.00587.b.A1.PW30194
PURIFICATION DATe: 11/29/2010
CONCENTRATION: 15.24mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 9
VIAL VOLUME: 100µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVPSQRRIL VVDDEPTICA SISARLRAEG YAVDTEDNGL DAVRHCRENP PDLMVLDVML PGLDGLEVCR QVQAHAPIPV LMLTARGTET DMLVGLGVGA DDYLPKPFSM RVLVARVAAL LRRTHRIAPP STDVRIGQVR IDVSERRVYH RDDEVHLTRT EFDLLAYLAD NPRVAISRDR LLDQVWGWSA GLPSGRRTVD SHIKALRRKL GQELIRTVHG IGYALEAPR
Validated NT Sequence
ttattatcgc ggtgcctcca acgcgtatcc gatgccgtgg acggtgcgga tcagctcctg accgagcttg cggcgcagcg ccttgatgtg actgtccacg gtgcgccgcc ccgagggcag ccccgcgctc cagccccaca cctgatcgag cagacgatca cgactgatgg ccacccgcgg gttgtcggcc aggtaggcca gtaggtcgaa ttcggtgcgg gtcaggtgca cctcgtcgtc gcggtgatat acgcggcgct cggacacgtc gatgcgtacc tgcccgatgc gcacatcggt gctcggcggc gcgatccgat gggtgcggcg cagcagcgcg gccacccggg ccaccagcac ccgcatgctg aagggcttgg gcaggtagtc gtcggccccc acccccaggc ccaccagcat gtcggtttcg gtgccccgcg cggtgagcat cagcaccggg atgggtgcgt gggcctgcac ctggcggcac acctccagcc cgtcgagtcc gggcagcatg acatccagca ccatcaggtc cggcgggttt tcgcggcaat gccgcaccgc gtccaggccg ttgtcctcgg tgtctaccgc atacccctcg gcgcgcagcc gtgcggaaat ggacgcacag atcgtgggtt cgtcgtcaac gaccaagatt cttcgctggc tgggcaccat cgaaccagga ccctgggtct gagcttccag ggtacccata tgatggtgat ggtgatgagc cat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVPSQRRIL VVDDEPTICA SISARLRAEG YAVDTEDNGL DAVRHCRENP PDLMVLDVML PGLDGLEVCR QVQAHAPIPV LMLTARGTET DMLVGLGVGA DDYLPKPFSM RVLVARVAAL LRRTHRIAPP STDVRIGQVR IDVSERRVYH RDDEVHLTRT EFDLLAYLAD NPRVAISRDR LLDQVWGWSA GLPSGRRTVD SHIKALRRKL GQELIRTVHG IGYALEAPR
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGCCCAGC CAGCGAAGAA TCTTGGTCGT TGACGACGAA CCCACGATCT GTGCGTCCAT TTCCGCACGG CTGCGCGCCG AGGGGTATGC GGTAGACACC GAGGACAACG GCCTGGACGC GGTGCGGCAT TGCCGCGAAA ACCCGCCGGA CCTGATGGTG CTGGATGTCA TGCTGCCCGG ACTCGACGGG CTGGAGGTGT GCCGCCAGGT GCAGGCCCAC GCACCCATCC CGGTGCTGAT GCTCACCGCG CGGGGCACCG AAACCGACAT GCTGGTGGGC CTGGGGGTGG GGGCCGACGA CTACCTGCCC AAGCCCTTCA GCATGCGGGT GCTGGTGGCC CGGGTGGCCG CGCTGCTGCG CCGCACCCAT CGGATCGCGC CGCCGAGCAC CGATGTGCGC ATCGGGCAGG TACGCATCGA CGTGTCCGAG CGCCGCGTAT ATCACCGCGA CGACGAGGTG CACCTGACCC GCACCGAATT CGACCTACTG GCCTACCTGG CCGACAACCC GCGGGTGGCC ATCAGTCGTG ATCGTCTGCT CGATCAGGTG TGGGGCTGGA GCGCGGGGCT GCCCTCGGGG CGGCGCACCG TGGACAGTCA CATCAAGGCG CTGCGCCGCA AGCTCGGTCA GGAGCTGATC CGCACCGTCC ACGGCATCGG ATACGCGTTG GAGGCACCGC GATAAACAGC ACGAACAAGT TCTGCAGCCA AGCTTCTCGA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAA