MytuD.00277.a

POSSIBLE ESTERASE LIPW

CENTER ID: MytuD.00277.a
ORGANISM: Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE: Tuberculosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MytuD.00277.a.A1.GE45906 Full length( MytuD.00277.a ) 1 302
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|83332.12.peg.244
RefSeq: NP_214731.1
TubercuList: Rv0217c
MTB Network Portal: Rv0217c
UniProt: P96399

Orthologues for Rv0217c

Species % Identity % Coverage Clones Proteins Structures
avium 74 97 0 0 0
paratuberculosis 73 98 1 0 0
smegmatis 65 97 0 0 0
abscessus 58 98 1 0 0
avium 51 97 0 0 0
marinum 51 97 0 0 0
paratuberculosis 51 97 1 1 0
ulcerans 48 72 1 0 0
avium 46 80 1 0 0
paratuberculosis 46 80 0 0 0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSGNEVHPDL RRIAVVTPRQ LVGPRTLPVM RALIVVAGLR MSRTPPDIEV LTLESGVGVR LYRPAGSNEP APALLWIHAG GYVMGTAQQD DRLCLRFSSR LGITVASVDY RLAPENPYPA ALGDCYSALT WLASLPAVDP ARVAIGGASA GGGLAAALAL LARDRGGITP AFQLLVYPML DDRPSIAPAN PHYRLWNGRA NRFGWRAYLG DADARVAVPG RRDDLGGLAP AWIGVGTHDL LHDEDLAYAE RLTAAGVPCQ VEVVEGAFHG FDRVAPNVGV SQRFFTSQCN SLRAALALSN RT
NT Sequence
GTGTCCGGCA ACGAAGTCCA CCCCGACCTG CGTCGCATCG CCGTCGTCAC CCCACGACAG CTGGTCGGTC CTCGCACCCT GCCAGTCATG CGGGCATTGA TCGTCGTCGC GGGGCTTCGA ATGTCCCGTA CACCCCCCGA TATCGAGGTG CTCACCCTGG AATCCGGGGT CGGTGTCCGG CTATACCGAC CCGCCGGCAG CAACGAACCA GCGCCCGCGC TGCTGTGGAT CCACGCCGGC GGATACGTAA TGGGCACCGC GCAACAGGAC GATCGGCTCT GCCTCCGGTT CAGCAGCAGA CTGGGCATCA CTGTCGCATC GGTGGACTAC CGCCTGGCGC CGGAAAATCC GTATCCTGCC GCCCTGGGCG ACTGCTACTC GGCGTTGACC TGGCTGGCCA GCCTGCCGGC GGTGGACCCC GCGCGGGTGG CAATCGGCGG CGCTAGTGCC GGCGGCGGCC TCGCGGCGGC GCTGGCTCTG CTTGCCCGCG ACCGTGGCGG CATCACACCG GCATTTCAGT TGCTGGTGTA CCCCATGCTC GACGACCGGC CGTCGATCGC CCCCGCCAAC CCGCACTACC GGCTATGGAA CGGGCGCGCC AACCGGTTCG GCTGGCGGGC ATATCTTGGC GATGCGGACG CACGGGTCGC GGTCCCGGGA CGGCGTGACG ACCTAGGAGG GCTGGCGCCG GCATGGATCG GCGTCGGCAC CCACGACCTG CTCCACGACG AGGATCTGGC CTATGCCGAA CGTCTGACAG CAGCCGGGGT GCCATGCCAG GTAGAAGTAG TCGAAGGCGC CTTCCACGGT TTCGATCGTG TGGCCCCGAA TGTTGGGGTC TCACAAAGGT TCTTCACTAG TCAGTGCAAC AGTTTGCGTG CCGCGCTGGC CCTTAGTAAC CGAACC
Details for MytuD.00277.a.A1.GE45906
HARVESTED ON: 5/13/2025
SEQUENCED ON: data unavailable
EXPECTED MW: 35kDa
OBSERVED MW: 35kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 96
PERCENT COVERAGE: 94
Validated AA Sequence
MVSGNEVHPD LRRIAVVTPR QLVGPRTLPV MRALIVVAGL XMSRTPPDIE VLTLESGVGV RLYRPAGSNE PAPALLWIHA GGYVMGTAQQ DDRLCLRFSS RLGITVASVD YRLAPENPYP AALGDCYSAL TWLASLPAVD PARVXIGGAS AGGGLAAALA LLARDRGGIT PAFQLLVYPM LDDRXSIAPA XPHYRXWNGR ANRFGWRAYL XDADARVAVP GRRDDXGGLA PAWIGVGTHD LLHDEDLAYA ERLTAXGVPC QVEVVEGAFH GFDRVAPNVG VXQRFXXSQC NSLRAALALS NRTXX
Validated NT Sequence
tnnantaggt tcggttacta agggccagcg cggcacgcaa actgttgcac tgactannnn agaacctttg tnagacccca acattcgggg ccacacgatc gaaaccgtgg aaggcgcctt cnactacttc tacctggcat ggcaccccgg ntgctgtcag acgttcggca tangccagat cctcgtcgtg gagcaggtcg tgggtgccga cgccgatcca tgccggcgcc agccctccta ngtcgtcacg ccgtcccggg accgcgaccc gtgcgtccgc atcnncaaga tatgcccgcc agccgaaccg gttggcgcgc ccgttccata nccggtagtg cgggtnggcg ggggcgatcg acgnccggtc gtcgagcatg gggtacacca gcaactgaaa tgccggtgtg atgccgccac ggtcgcgggc aagcagagcc agcgccgccg cgaggccgcc gccggcacta gcgccgccga ttnccacccg cgcggggtcc accgccggca ggctggccag ccaggtcaac gccgagtagc agtcgcccag ggcggcagga tacggatttt ccggcgccag gcggtagtcc accgatgcga cagtgatgcc cagtctgctg ctgaaccgga ggcagagccg atcgtcctgt tgcgcggtgc ccattacgta tccgccggcg tggatccaca gcagcgcggg cgctggttcg ttgctgccgg cgggtcggta tagccggaca ccgaccccgg attccagggt gagcacctcg atatcggggg gngtacggga cattngaagc cccgcgacga cgatcaatgc ccgcatgact ggcagggtgc gaggaccgac cagctgtcgt ggggtgacga cggcgatgcg acgcaggtcg gggtggactt cgttgccgga caccatcgaa ccaggaccct gggtctgagc ttccaggg
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVSGNEVHP DLRRIAVVTP RQLVGPRTLP VMRALIVVAG LRMSRTPPDI EVLTLESGVG VRLYRPAGSN EPAPALLWIH AGGYVMGTAQ QDDRLCLRFS SRLGITVASV DYRLAPENPY PAALGDCYSA LTWLASLPAV DPARVAIGGA SAGGGLAAAL ALLARDRGGI TPAFQLLVYP MLDDRPSIAP ANPHYRLWNG RANRFGWRAY LGDADARVAV PGRRDDLGGL APAWIGVGTH DLLHDEDLAY AERLTAAGVP CQVEVVEGAF HGFDRVAPNV GVSQRFFTSQ CNSLRAALAL SNRT
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgtccggc aacgaagtcc accccgacct gcgtcgcatc gccgtcgtca ccccacgaca gctggtcggt cctcgcaccc tgccagtcat gcgggcattg atcgtcgtcg cggggcttcg aatgtcccgt acaccccccg atatcgaggt gctcaccctg gaatccgggg tcggtgtccg gctataccga cccgccggca gcaacgaacc agcgcccgcg ctgctgtgga tccacgccgg cggatacgta atgggcaccg cgcaacagga cgatcggctc tgcctccggt tcagcagcag actgggcatc actgtcgcat cggtggacta ccgcctggcg ccggaaaatc cgtatcctgc cgccctgggc gactgctact cggcgttgac ctggctggcc agcctgccgg cggtggaccc cgcgcgggtg gcaatcggcg gcgctagtgc cggcggcggc ctcgcggcgg cgctggctct gcttgcccgc gaccgtggcg gcatcacacc ggcatttcag ttgctggtgt accccatgct cgacgaccgg ccgtcgatcg cccccgccaa cccgcactac cggctatgga acgggcgcgc caaccggttc ggctggcggg catatcttgg cgatgcggac gcacgggtcg cggtcccggg acggcgtgac gacctaggag ggctggcgcc ggcatggatc ggcgtcggca cccacgacct gctccacgac gaggatctgg cctatgccga acgtctgaca gcagccgggg tgccatgcca ggtagaagta gtcgaaggcg ccttccacgg tttcgatcgt gtggccccga atgttggggt ctcacaaagg ttcttcacta gtcagtgcaa cagtttgcgt gccgcgctgg cccttagtaa ccgaaccaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa