SSGCID: Target Page

MypaA.00277.c

Putative uncharacterized protein

CENTER ID:	MypaA.00277.c
ORGANISM:	Mycobacterium paratuberculosis ATCC BAA-968 / K-10
ASSOCIATED DISEASE:	Johne's disease
CURRENT STATUS:	diffraction
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MypaA.00277.c.AE1.GE43239	Full length( MypaA.00277.c )		1	308

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MypaA.00277.c.A1.PS00844	Full length( MypaA.00277.c )		1	308

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|262316.17.peg.1840
RefSeq:	NP_960669.1
UniProt:	Q73Z68

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MADRLPDDAF TAPQVVDPGL RKVARFLPRG YALHRGLKLQ RAIMNLMGNA GRLRTVPVVA VNEHVTVRLH RPPNLAEHAP ALLWIHGGGT VMGRAAQDDK YLRKLSHRTG VAIAAVEHRL APEHPYPAPV EDCYAALLWL ARQPWVDPER VAVGGASAGG LFAATLAQRA HDRNDVKIAF QMLVYPMLDD RTGAQRDGPR RLMWTESDNQ LAWQWYLNGA DPDEAAPARR KDLSGLAPAW IGVGTLDLFY PECLEYARRL REAGVPAQEE IVPGAFHAFD QIVDKAPISA KFFESQCDFL RGALAPNG

NT Sequence

ATGGCTGACC GGCTCCCCGA CGATGCCTTC ACGGCGCCCC AGGTGGTGGA TCCCGGTCTG CGCAAGGTGG CTCGATTCCT GCCCCGCGGG TACGCCCTGC ACCGCGGTCT CAAGCTGCAG CGCGCCATCA TGAACCTGAT GGGCAACGCG GGCCGCCTCC GCACGGTGCC GGTGGTCGCG GTCAACGAGC ACGTCACCGT CCGGCTGCAC CGTCCACCCA ACCTCGCCGA GCATGCGCCC GCGCTGCTCT GGATCCACGG CGGCGGCACG GTCATGGGGC GCGCCGCCCA GGACGACAAG TACCTGCGCA AGCTTTCGCA CCGCACCGGT GTCGCGATCG CGGCGGTGGA ACACCGGTTG GCTCCCGAAC ACCCGTACCC CGCGCCGGTC GAGGACTGTT ACGCGGCCCT GCTGTGGCTG GCGCGCCAGC CCTGGGTGGA TCCGGAGCGG GTCGCCGTCG GTGGGGCAAG TGCCGGTGGA CTTTTCGCGG CCACCCTGGC GCAGCGGGCG CACGACCGCA ACGACGTCAA AATCGCCTTC CAGATGCTGG TGTATCCGAT GCTCGACGAT CGCACCGGCG CCCAACGCGA CGGCCCCCGA CGCCTCATGT GGACCGAATC CGACAACCAG CTGGCCTGGC AGTGGTACCT CAACGGGGCC GATCCCGACG AGGCGGCACC GGCCCGCCGC AAGGACCTGT CGGGCTTGGC GCCGGCCTGG ATCGGCGTCG GCACCCTGGA CCTCTTTTAT CCGGAGTGCC TGGAGTACGC GCGGCGTCTG CGCGAGGCCG GCGTTCCAGC GCAGGAGGAG ATCGTCCCCG GCGCGTTTCA CGCCTTCGAC CAGATCGTGG ACAAGGCGCC GATCTCCGCC AAATTCTTCG AAAGTCAGTG CGATTTTCTG CGCGGTGCTC TCGCCCCCAA CGGG

Details for MypaA.00277.c.AE1.GE43239

HARVESTED ON:	6/12/2019
SEQUENCED ON:	6/18/2019
EXPECTED MW:	35kDa
OBSERVED MW:	35kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atttgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgag atcccggtgc ctaatgagtg agctaactta cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc cagggtggtt tttcttttca ccagtgagac gggcaacagc tgattgccct tcaccgcctg gccctgagag agttgcagca agcggtccac gctggtttgc cccagcaggc gaaaatcctg tttgatggtg gttaacggcg ggatataaca tgagctgtct tcggtatcgt cgtatcccac taccgagata tccgcaccaa cgcgcagccc ggactcggta atggcgcgca ttgcgcccag cgccatctga tcgttggcaa ccagcatcgc agtgggaacg atgccctcat tcagcatttg catggtttgt tgaaaaccgg acatggcact ccagtcgcct tcccgttccg ctatcggctg aatttgattg cgagtgagat atttatgcca gccagccaga cgcagacgcg ccgagacaga acttaatggg cccgctaaca gcgcgatttg ctggtgaccc aatgcgacca gatgctccac gcccagtcgc gtaccgtctt catgggagaa aataatactg ttgatgggtg tctggtcaga gacatcaaga aataacgccg gaacattagt gcaggcagct tccacagcaa tggcatcctg gtcatccagc ggatagttaa tgatcagccc actgacgcgt tgcgcgagaa gattgtgcac cgccgcttta caggcttcga cgccgcttcg ttctaccatc gacaccacca cgctggcacc cagttgatcg gcgcgagatt taatcgccgc gacaatttgc gacggcgcgt gcagggccag actggaggtg gcaacgccaa tcagcaacga ctgtttgccc gccagttgtt gtgccacgcg gttgggaatg taattcagct ccgccatcgc cgcttccact ttttcccgcg ttttcgcaga aacgtggctg gcctggttca ccacgcggga aacggtctga taagagacac cggcatactc tgcgacatcg tataacgtta ctggtttcac attcaccacc ctgaattgac tctcttccgg gcgctatcat gccataccgc gaaaggtttt gcgccattcg atggtgtccg ggatctcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat cgatctcgat cccgcgaaat taatacgact cactataggg gaattgtgag cggataacaa ttcccctcta gaaataattt tgtttaactt taagaaggag tctctcccat ggctgaccgg ctccccgacg atgccttcac ggcgccccag gtggtggatc ccggtctgcg caaggtggct cgattcctgc cccgcgggta cgccctgcac cgcggtctca agctgcagcg cgccatcatg aacctgatgg gcaacgcggg ccgcctccgc acggtgccgg tggtcgcggt caacgagcac gtcaccgtcc ggctgcaccg tccacccaac ctcgccgagc atgcgcccgc gctgctctgg atccacggcg gcggcacggt catggggcgc gccgcccagg acgacaagta cctgcgcaag ctttcgcacc gcaccggtgt cgcgatcgcg gcggtggaac accggttggc tcccgaacac ccgtaccccg cgccggtcga ggactgttac gcggccctgc tgtggctggc gcgccagccc tgggtggatc cggagcgggt cgccgtcggt ggggcaagtg ccggtggact tttcgcggcc accctggcgc agcgggcgca cgaccgcaac gacgtcaaaa tcgccttcca gatgctggtg tatccgatgc tcgacgatcg caccggcgcc caacgcgacg gcccccgacg cctcatgtgg accgaatccg acaaccagct ggcctggcag tggtacctca acggggccga tcccgacgag gcggcaccgg cccgccgcaa ggacctgtcg ggcttggcgc cggcctggat cggcgtcggc accctggacc tcttttatcc ggagtgcctg gagtacgcgc ggcgtctgcg cgaggccggc gttccagcgc aggaggagat cgtccccggc gcgtttcacg ccttcgacca gatcgtggac aaggcgccga tctccgccaa attcttcgaa agtcagtgcg attttctgcg cggtgctctc gcccccaacg gggggcacca ccatcatcat cattaacgga tccgaattcg agctccgtcg acaagcttgc ggccgcactc gagcaccacc accaccacca ctgagatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat

Details for MypaA.00277.c.A1.PS00844

PURIFICATION DATe:	11/8/2010
CONCENTRATION:	31.38mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	2
VIAL VOLUME:	200µl
PERCENT IDENTITY:	99
PERCENT COVERAGE:	90

Protocol Notes

notes unavailable

Validated AA Sequence

APQVVXPXLR KVARFLPRGY ALHRGLKXQR AIMXLMGNAX RLRTVPVVAV NEHVTVRLHR PPNLAEHAPA LLWIHGGGTV MGRAAQDDKY LRKLSHRTGV AIAAVEHRLA PEHPYPAPVE DCYAALLWLA RQPWVDPERV AVGGASAGGL FAATLAQRAH DRNDVKIAFQ MLVYPMLDDR TGAQRDGPRR LMWTESDNQL AWQWYLNGAD PDEAAPARRK DLSGLAPAWI GVGTLDLFYP ECLEYARRLR EAGVPAQEEI VPGAFHAFDQ IVDKAPISAK FFESQCDFLR GALAPNG

Validated NT Sequence

nnnnnnnnnn nnnnnnnnnn nnntcnttnn ggnttngtnn gcagccggat cctcgngaag cttggctgca gaacttgttc gtgctgttta ttacccgttg ggggcgagag caccgcgcag aaaatcgcac tgactttcga agaatttggc ggagatcggc gccttgtcca cgatctggtc gaaggcgtga aacgcgccgg ggacgatctc ctcctgcgct ggaacgccgg cctcgcgcag acgccgcgcg tactccaggc actccggata aaagaggtcc agggtgccga cgccgatcca ggccggcgcc aagcccgaca ggtccttgcg gcgggccggt gccgcctcgt cgggatcggc cccgttgagg taccactgcc aggccagctg gttgtcggat tcggtccaca tgaggcgtcg ggggccgtcg cgttgggcgc cggtgcgatc gtcgagcatc ggatacacca gcatctggaa ggcgattttg acgtcgttgc ggtcgtgcgc ccgctgcgcc agggtggccg cgaaaagtcc accggcactt gccccaccga cggcgacccg ctccggatcc acccagggct ggcgcgccag ccacagcagg gccgcgtaac agtcctcgac cggcgcgggg tacgggtgtt cgggagccaa ccggtgttcc accgccgcga tcgcgacacc ggtgcggtgc gaaagcttgc gcaggtactt gtcgtcctgg gcggcgcgcc ccatgaccgt gccgccgccg tggatccaga gcagcgcggg cgcatgctcg gcgaggttgg gtggacggtg cagccggacg gtgacgtgct cgttgaccgc gaccaccggc accgtgcgga ggcggnccgc gttgcccatc aggnncatga tggcgcgctg cancttgaga ccgcggtgca gggcgtaccc gcggggcagg aatcgagcca ccttgcgcag acngggntcc accacctggg gcgccgngan nnatcgtnng gnnccgntca gccatcnaac cnggacccng ggtctganct tcnngnnccc atatgangnn gatgnngatg ancatgnnnn tcnnnnnnan nnnaannntt nnanangnnn ngnnnnngnn ncnnnnnnnn nnnnnnnnnn nnannnnnnn nn

Expressed Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMADRLPDDA FTAPQVVDPG LRKVARFLPR GYALHRGLKL QRAIMNLMGN AGRLRTVPVV AVNEHVTVRL HRPPNLAEHA PALLWIHGGG TVMGRAAQDD KYLRKLSHRT GVAIAAVEHR LAPEHPYPAP VEDCYAALLW LARQPWVDPE RVAVGGASAG GLFAATLAQR AHDRNDVKIA FQMLVYPMLD DRTGAQRDGP RRLMWTESDN QLAWQWYLNG ADPDEAAPAR RKDLSGLAPA WIGVGTLDLF YPECLEYARR LREAGVPAQE EIVPGAFHAF DQIVDKAPIS AKFFESQCDF LRGALAPNG

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGCTGACCGG CTCCCCGACG ATGCCTTCAC GGCGCCCCAG GTGGTGGATC CCGGTCTGCG CAAGGTGGCT CGATTCCTGC CCCGCGGGTA CGCCCTGCAC CGCGGTCTCA AGCTGCAGCG CGCCATCATG AACCTGATGG GCAACGCGGG CCGCCTCCGC ACGGTGCCGG TGGTCGCGGT CAACGAGCAC GTCACCGTCC GGCTGCACCG TCCACCCAAC CTCGCCGAGC ATGCGCCCGC GCTGCTCTGG ATCCACGGCG GCGGCACGGT CATGGGGCGC GCCGCCCAGG ACGACAAGTA CCTGCGCAAG CTTTCGCACC GCACCGGTGT CGCGATCGCG GCGGTGGAAC ACCGGTTGGC TCCCGAACAC CCGTACCCCG CGCCGGTCGA GGACTGTTAC GCGGCCCTGC TGTGGCTGGC GCGCCAGCCC TGGGTGGATC CGGAGCGGGT CGCCGTCGGT GGGGCAAGTG CCGGTGGACT TTTCGCGGCC ACCCTGGCGC AGCGGGCGCA CGACCGCAAC GACGTCAAAA TCGCCTTCCA GATGCTGGTG TATCCGATGC TCGACGATCG CACCGGCGCC CAACGCGACG GCCCCCGACG CCTCATGTGG ACCGAATCCG ACAACCAGCT GGCCTGGCAG TGGTACCTCA ACGGGGCCGA TCCCGACGAG GCGGCACCGG CCCGCCGCAA GGACCTGTCG GGCTTGGCGC CGGCCTGGAT CGGCGTCGGC ACCCTGGACC TCTTTTATCC GGAGTGCCTG GAGTACGCGC GGCGTCTGCG CGAGGCCGGC GTTCCAGCGC AGGAGGAGAT CGTCCCCGGC GCGTTTCACG CCTTCGACCA GATCGTGGAC AAGGCGCCGA TCTCCGCCAA ATTCTTCGAA AGTCAGTGCG ATTTTCTGCG CGGTGCTCTC GCCCCCAACG GGAAACAGCA CGAACAAGTT CTGCAGCCAA GCTTCTCGAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAA