MypaA.00277.a

LipW

CENTER ID: MypaA.00277.a
ORGANISM: Mycobacterium paratuberculosis ATCC BAA-968 / K-10
ASSOCIATED DISEASE: Johne's disease
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MypaA.00277.a.A1.GE29415 Full length( MypaA.00277.a ) 1 299
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|262316.17.peg.3885
RefSeq: NP_962589.1
UniProt: Q73TR3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MPNNDVHPDL QRFARFAPRR LVGPRTLPLM RVALAWRGRL GKPVRDVEVI TLGSGVGVRL FRPAGAAETT PALLWIHGGG YVLGTAQQDD QLCSRFSRRL GITVASVDYR LAPEHRYPAP LEDCYTALTW LAGLPSVDRY GIAIGGASAG GGLAAALALL ARDRAEVSPA FQLLTYPMLD DRSSDTAPSP NYRLWDNRSN RFGWAAYLGD ADPHVAVPAR RDDLSGLPPA WIGVGTHDLF HDEDLAYAER LRAAGVPCQV ETIPGAFHGF DLILPKVQVS QRFFDSQCNS LRAALTPAG
NT Sequence
GTGCCGAACA ACGATGTTCA TCCTGACCTG CAACGATTTG CCCGGTTCGC GCCGCGACGA CTGGTCGGCC CGCGAACGCT GCCGCTGATG CGGGTGGCGC TTGCCTGGCG CGGGCGGCTG GGCAAGCCGG TCCGCGACGT CGAGGTGATC ACGCTCGGTT CCGGGGTGGG GGTCCGCCTG TTCCGGCCCG CCGGCGCCGC GGAGACGACG CCCGCGCTGC TGTGGATCCA CGGCGGCGGC TACGTGCTGG GCACCGCCCA GCAGGACGAC CAGCTGTGCA GCCGGTTCAG CCGACGGCTG GGCATCACGG TCGCATCGGT GGATTACCGC CTGGCGCCCG AACATCGGTA TCCGGCGCCG CTGGAGGACT GCTATACGGC GCTGACCTGG CTGGCCGGTC TGCCGTCGGT GGACCGCTAC GGCATCGCCA TCGGCGGGGC CAGCGCCGGC GGCGGCCTGG CCGCGGCGCT GGCGCTGCTG GCCCGCGACC GCGCCGAGGT GAGCCCCGCA TTCCAGCTGC TGACCTATCC CATGCTCGAC GACCGCAGCT CGGACACCGC GCCCAGCCCG AACTATCGGC TGTGGGACAA CCGCAGCAAC AGGTTCGGCT GGGCGGCCTA CCTCGGCGAC GCCGACCCGC ACGTCGCGGT GCCCGCCCGG CGGGACGACC TCAGCGGACT GCCGCCGGCC TGGATCGGGG TGGGCACCCA TGACCTGTTT CACGACGAGG ACCTCGCCTA CGCCGAACGG CTGCGGGCCG CCGGGGTGCC CTGCCAGGTG GAGACCATCC CCGGCGCCTT CCACGGCTTC GACCTGATTT TGCCGAAAGT TCAAGTGTCG CAGCGGTTTT TCGACAGCCA GTGCAACAGC CTGCGGGCCG CGCTGACGCC GGCCGGC
Details for MypaA.00277.a.A1.GE29415
HARVESTED ON: 5/27/2010
SEQUENCED ON: 6/14/2010
EXPECTED MW: 34kDa
OBSERVED MW: 35kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 79
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVPNNDVHP DLQRFARFAP RRLVGPRTLP LMRVALAWRG RLGKPVRDVE VITLGSGVGV RLFRPAGAAE TTPALLWIHG GGYVLGTAQQ DDQLCSRFSR RLGITVASVD YRLAPEHRYP APLEDCYTAL TWLAGLPSVD RYGIAIGGAS AGGGLAAALA LLARDRAEVS PAFQLLTYPM LDDRSSDTAP SPNYRLWDNR SNRFGWAAYL GDADPHVAVP ARRDDLSGCR RXGSGWXPMX CFTTRTSPTP NGCGPPGCPA RGXXPXRLPR XXXDFAXSSX VXAXFRXPVQ XPXXRXDAXG
Validated NT Sequence
annnnttttg tttnncttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgccgaac aacgatgttc atcctgacct gcaacgattt gcccggttcg cgccgcgacg actggtcggc ccgcgaacgc tgccgctgat gcgggtggcg cttgcctggc gcgggcggct gggcaagccg gtccgcgacg tcgaggtgat cacgctcggt tccggggtgg gggtccgcct gttccggccc gccggcgccg cggagacgac gcccgcgctg ctgtggatcc acggcggcgg ctacgtgctg ggcaccgccc agcaggacga ccagctgtgc agccggttca gccgacggct gggcatcacg gtcgcatcgg tggattaccg cctggcgccc gaacatcggt atccggcgcc gctggaggac tgctatacgg cgctgacctg gctggccggt ctgccgtcgg tggaccgcta cggcatcgcc atcggcgggg ccagcgccgg cggcggcctg gccgcggcgc tggcgctgct ggcccgcgac cgcgccgagg tgagccccgc attccagctg ctgacctatc ccatgctcga cgaccgcagc tcggacaccg cgcccagccc gaactatcgg ctgtgggaca accgcagcaa caggttcggc tgggcggcct acctcggcga cgccgacccg cacgtcgcgg tgcccgcccg gcgggacgac ctcagcggat gccgccggnc tggatcgggg tgggnnccca tganctgttt cacgacgagg acctcgccta cnccgaacgg ctgcgggccg ccggggtgcc ctgccagggg anacnatccc nggcgccttc cacggnttcn anctgatttt gccnaaagtt cnngtgtcnc agcngntttt cgannnccag tgcaanngcc ngnggnncgc gntgacgccn ncggntaata aacagcacga anaannnnng cagnccttct cgagnncnng nnncnaacaa ancccgaang nnnnnnnant nnncnnnnnn c
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVPNNDVHP DLQRFARFAP RRLVGPRTLP LMRVALAWRG RLGKPVRDVE VITLGSGVGV RLFRPAGAAE TTPALLWIHG GGYVLGTAQQ DDQLCSRFSR RLGITVASVD YRLAPEHRYP APLEDCYTAL TWLAGLPSVD RYGIAIGGAS AGGGLAAALA LLARDRAEVS PAFQLLTYPM LDDRSSDTAP SPNYRLWDNR SNRFGWAAYL GDADPHVAVP ARRDDLSGLP PAWIGVGTHD LFHDEDLAYA ERLRAAGVPC QVETIPGAFH GFDLILPKVQ VSQRFFDSQC NSLRAALTPA G
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgccgaac aacgatgttc atcctgacct gcaacgattt gcccggttcg cgccgcgacg actggtcggc ccgcgaacgc tgccgctgat gcgggtggcg cttgcctggc gcgggcggct gggcaagccg gtccgcgacg tcgaggtgat cacgctcggt tccggggtgg gggtccgcct gttccggccc gccggcgccg cggagacgac gcccgcgctg ctgtggatcc acggcggcgg ctacgtgctg ggcaccgccc agcaggacga ccagctgtgc agccggttca gccgacggct gggcatcacg gtcgcatcgg tggattaccg cctggcgccc gaacatcggt atccggcgcc gctggaggac tgctatacgg cgctgacctg gctggccggt ctgccgtcgg tggaccgcta cggcatcgcc atcggcgggg ccagcgccgg cggcggcctg gccgcggcgc tggcgctgct ggcccgcgac cgcgccgagg tgagccccgc attccagctg ctgacctatc ccatgctcga cgaccgcagc tcggacaccg cgcccagccc gaactatcgg ctgtgggaca accgcagcaa caggttcggc tgggcggcct acctcggcga cgccgacccg cacgtcgcgg tgcccgcccg gcgggacgac ctcagcggac tgccgccggc ctggatcggg gtgggcaccc atgacctgtt tcacgacgag gacctcgcct acgccgaacg gctgcgggcc gccggggtgc cctgccaggt ggagaccatc cccggcgcct tccacggctt cgacctgatt ttgccgaaag ttcaagtgtc gcagcggttt ttcgacagcc agtgcaacag cctgcgggcc gcgctgacgc cggccggcaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa