SSGCID: Target Page

MyulA.00277.c

Esterase/lipase

CENTER ID:	MyulA.00277.c
ORGANISM:	Mycobacterium ulcerans Agy99
ASSOCIATED DISEASE:	Buruli, Bairnsdale ulcer
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyulA.00277.c.A1.GE29671	Full length( MyulA.00277.c )		1	240

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|362242.7.peg.3661
RefSeq:	YP_906838.1
UniProt:	A0PSP8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MRTPRCAIVW CMQARPDGPP AFTKPDAIDP ALRAISRILP RGYGLHRGLK LPRALMKLAG ITGRLRDVPV VAVNDAVSVR SHRPAGLPDP GPALLWIHGG GTVMGSAAQE DQYCRKLSHL AGVAVAAVDY RLAPEHPYPT PVEDCYAALL WLRQQQWVDP SRIAVAGASA GDLFAATLVQ LACDRGDVEP VLQMLVYPML DDRTGSGPNG HKRIMWSERD NQQAWQLYLA GADPSTAAPA

NT Sequence

ATGCGGACAC CTCGATGTGC AATCGTATGG TGCATGCAAG CCCGTCCCGA TGGTCCGCCG GCCTTCACCA AGCCCGACGC AATCGACCCC GCCTTGCGGG CGATCTCCCG GATCCTGCCG CGAGGCTATG GGTTGCATCG GGGCCTCAAG CTGCCACGTG CGCTGATGAA GCTGGCTGGG ATTACTGGAC GTCTGCGCGA TGTGCCGGTG GTGGCCGTCA ACGACGCGGT CAGCGTGCGA TCGCACCGGC CAGCCGGATT GCCGGATCCG GGCCCGGCCC TGCTCTGGAT CCACGGCGGC GGAACGGTCA TGGGCAGCGC CGCTCAGGAG GATCAGTACT GCCGCAAGTT GTCTCATCTG GCGGGCGTCG CGGTGGCCGC GGTGGACTAC CGGTTGGCGC CCGAGCACCC CTATCCGACC CCCGTCGAGG ACTGTTATGC GGCGTTGCTG TGGCTGCGCC AGCAGCAGTG GGTCGATCCG TCGCGGATCG CGGTGGCCGG TGCCAGTGCC GGCGATCTTT TCGCGGCCAC GCTGGTTCAG CTGGCCTGCG ACCGTGGTGA TGTGGAACCG GTGCTGCAGA TGTTGGTGTA CCCCATGCTC GACGACCGCA CCGGCTCCGG TCCGAACGGC CACAAGCGCA TCATGTGGAG CGAGCGGGAC AACCAACAGG CGTGGCAGTT GTATCTGGCC GGGGCAGACC CCAGCACGGC CGCACCGGCG

Details for MyulA.00277.c.A1.GE29671

HARVESTED ON:	6/22/2010
SEQUENCED ON:	7/1/2010
EXPECTED MW:	28kDa
OBSERVED MW:	28kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	96
PERCENT COVERAGE:	106

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMRTPRCAIV WCMQARPDGP PAFTKPDAID PALRAISRIL PRGYGLHRGL KLPRALMKLA GITGRLRDVP VVAVNDAVSV RSHRPAGLPD PGPALLWIHG GGTVMGSAAQ EDQYCRKLSH LAGVAVAAVD YRLAPEHPYP TPVEDCYAAL LWLRQQQWVD PSRIAVAGAS AGDLFAATLV QLACDRGDVE PVLQMLVYPM LDDRTGSGPN GHKRIMWSER DNQQAWQLYL XRGRPQHGRT GVINSTXXLQ XXXXXSGC

Validated NT Sequence

ttttgtttaa ctttaagaag gagatatacc atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgcgga cacctcgatg tgcaatcgta tggtgcatgc aagcccgtcc cgatggtccg ccggccttca ccaagcccga cgcaatcgac cccgccttgc gggcgatctc ccggatcctg ccgcgaggct atgggttgca tcggggcctc aagctgccac gtgcgctgat gaagctggct gggattactg gacgtctgcg cgatgtgccg gtggtggccg tcaacgacgc ggtcagcgtg cgatcgcacc ggccagccgg attgccggat ccgggcccgg ccctgctctg gatccacggc ggcggaacgg tcatgggcag cgccgctcag gaggatcagt actgccgcaa gttgtctcat ctggcgggcg tcgcggtggc cgcggtggac taccggttgg cgcccgagca cccctatccg acccccgtcg aggactgtta tgcggcgttg ctgtggctgc gccagcagca gtgggtcgat ccgtcgcgga tcgcggtggc cggtgccagt gccggcgatc ttttcgcggc cacgctggtt cagctggcct gcgaccgtgg tgatgtggaa ccggtgctgc agatgttggt gtaccccatg ctcgacgacc gcaccggctc cggtccgaac ggccacaagc gcatcatgtg gagcgagcgg gacaaccaac aggcgtggca gttgtatctg gnccggggca gaccccagca cggncgcacc ggcgtaataa acagcacgaa ngnnctgcag cnnnctnctc nangntccgg ctgctaacaa agcccgaaag gaagctgant tggctgctgc cnnngntna

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcggacacct cgatgtgcaa tcgtatggtg catgcaagcc cgtcccgatg gtccgccggc cttcaccaag cccgacgcaa tcgaccccgc cttgcgggcg atctcccgga tcctgccgcg aggctatggg ttgcatcggg gcctcaagct gccacgtgcg ctgatgaagc tggctgggat tactggacgt ctgcgcgatg tgccggtggt ggccgtcaac gacgcggtca gcgtgcgatc gcaccggcca gccggattgc cggatccggg cccggccctg ctctggatcc acggcggcgg aacggtcatg ggcagcgccg ctcaggagga tcagtactgc cgcaagttgt ctcatctggc gggcgtcgcg gtggccgcgg tggactaccg gttggcgccc gagcacccct atccgacccc cgtcgaggac tgttatgcgg cgttgctgtg gctgcgccag cagcagtggg tcgatccgtc gcggatcgcg gtggccggtg ccagtgccgg cgatcttttc gcggccacgc tggttcagct ggcctgcgac cgtggtgatg tggaaccggt gctgcagatg ttggtgtacc ccatgctcga cgaccgcacc ggctccggtc cgaacggcca caagcgcatc atgtggagcg agcgggacaa ccaacaggcg tggcagttgt atctggccgg ggcagacccc agcacggccg caccggcgaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa