MytuD.00236.a

PROBABLE CONSERVED ATP-BINDING PROTEIN ABC TRANSPORTER

CENTER ID: MytuD.00236.a
ORGANISM: Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE: Tuberculosis
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MytuD.00236.a.A1.GE25816 Full length( MytuD.00236.a ) 1 266
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MytuD.00236.a.A1.PW25865 Full length( MytuD.00236.a ) 1 266

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|83332.12.peg.1634
RefSeq: NP_215979.1
TubercuList: Rv1463
MTB Network Portal: Rv1463
UniProt: O53154

Orthologues for Rv1463

Species % Identity % Coverage Clones Proteins Structures
marinum 90 95 1 1 0
paratuberculosis 90 95 1 1 0
ulcerans 89 95 1 0 0
leprae 86 100 1 0 0
fortuitum 86 96 0 0 0
smegmatis 85 95 1 1 0
thermoresistibile 84 87 0 0 0
abscessus 83 96 1 0 0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTILEIKDLH VSVENPAEAD HEIPILRGVD LTVKSGETHA LMGPNGSGKS TLSYAIAGHP KYHVTSGTIT LDGADVLAMS IDERARAGLF LAMQYPVEVP GVSMSNFLRS AATAIRGEPP KLRHWVKEVK AAMAALDIDP AFAERSVNEG FSGGEKKRHE ILQLELLKPK IAILDETDSG LDVDALRVVS EGVNRYAESQ HGGILLITHY TRILRYIHPE YVHVFVGGRI VESGGSELAD ELDQNGYVRF SPASGRYPHQ PAPTGA
NT Sequence
ATGACCATTT TGGAAATTAA GGACCTGCAC GTCAGCGTGG AGAACCCCGC GGAGGCGGAC CACGAGATCC CGATCCTGCG CGGCGTCGAC CTCACCGTGA AATCCGGTGA GACACATGCC TTGATGGGAC CCAACGGCTC GGGCAAGTCG ACGCTGTCCT ACGCCATCGC GGGCCATCCC AAATACCACG TGACGTCGGG CACCATTACC CTCGACGGCG CGGACGTGCT GGCGATGAGC ATCGACGAAC GTGCGCGGGC CGGCCTGTTT CTGGCCATGC AATATCCCGT CGAGGTGCCC GGTGTCTCGA TGTCGAACTT CCTGCGCTCG GCGGCAACCG CCATTCGCGG CGAGCCGCCG AAACTGCGGC ACTGGGTCAA AGAGGTCAAG GCCGCGATGG CCGCGCTCGA CATCGACCCG GCCTTCGCCG AGCGCAGCGT CAACGAGGGT TTCTCCGGTG GCGAGAAGAA GCGCCACGAG ATCCTGCAGC TAGAACTGCT CAAGCCCAAG ATCGCCATCC TGGACGAGAC CGACTCCGGC CTGGACGTCG ACGCGCTGCG CGTGGTCAGC GAGGGGGTGA ACCGCTACGC CGAATCCCAG CACGGCGGCA TCCTGCTGAT CACGCACTAC ACCCGCATCC TGCGCTACAT CCACCCGGAA TACGTGCACG TGTTCGTCGG CGGCCGCATC GTCGAGTCCG GTGGTTCGGA GCTCGCCGAC GAACTCGACC AGAACGGCTA CGTGCGTTTC TCCCCCGCAA GCGGGCGGTA CCCCCACCAA CCCGCGCCAA CCGGAGCC
Details for MytuD.00236.a.A1.GE25816
HARVESTED ON: 11/19/2008
SEQUENCED ON: 8/18/2010
EXPECTED MW: 31kDa
OBSERVED MW: 31kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTILEIKDL HVSVENPAEA DHEIPILRGV DLTVKSGETH ALMGPNGSGK STLSYAIAGH PKYHVTSGTI TLDGADVLAM SIDERARAGL FLAMQYPVEV PGVSMSNFLR SAATAIRGEP PKLRHWVKEV KAAMAALDID PAFAERSVNE GFSGGEKKRH EILQLELLKP KIAILDETDS GLDVDALRVV SEGVNRYAES QHGGILLITH YTRILRYIHP EYVHVFVGGR IVESGGSELA DELDQNGYVR FSPASGRYPH QPAPTGA
Validated NT Sequence
tttaagaagg agatatacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatgaccat tttggaaatt aaggacctgc acgtcagcgt ggagaacccc gcggaggcgg accacgagat cccgatcctg cgcggcgtcg acctcaccgt gaaatccggt gagacacatg ccttgatggg acccaacggc tcgggcaagt cgacgctgtc ctacgccatc gcgggccatc ccaaatacca cgtgacgtcg ggcaccatta ccctcgacgg cgcggacgtg ctggcgatga gcatcgacga acgtgcgcgg gccggcctgt ttctggccat gcaatatccc gtcgaggtgc ccggtgtctc gatgtcgaac ttcctgcgct cggcggcaac cgccattcgc ggcgagccgc cgaaactgcg gcactgggtc aaagaggtca aggccgcgat ggccgcgctc gacatcgacc cggccttcgc cgagcgcagc gtcaacgagg gtttctccgg tggcgagaag aagcgccacg agatcctgca gctagaactg ctcaagccca agatcgccat cctggacgag accgactccg gcctggacgt cgacgcgctg cgcgtggtca gcgagggggt gaaccgctac gccgaatccc agcacggcgg catcctgctg atcacgcact acacccgcat cctgcgctac atccacccgg aatacgtgca cgtgttcgtc ggcggccgca tcgtcgagtc cggtggttcg gagctcgccg acgaactcga ccagaacggc tacgtgcgtt tctcccccgc aagcgggcgg tacccccacc aacccgcgcc aaccggagcc taataa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTILEIKDL HVSVENPAEA DHEIPILRGV DLTVKSGETH ALMGPNGSGK STLSYAIAGH PKYHVTSGTI TLDGADVLAM SIDERARAGL FLAMQYPVEV PGVSMSNFLR SAATAIRGEP PKLRHWVKEV KAAMAALDID PAFAERSVNE GFSGGEKKRH EILQLELLKP KIAILDETDS GLDVDALRVV SEGVNRYAES QHGGILLITH YTRILRYIHP EYVHVFVGGR IVESGGSELA DELDQNGYVR FSPASGRYPH QPAPTGA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaccattttg gaaattaagg acctgcacgt cagcgtggag aaccccgcgg aggcggacca cgagatcccg atcctgcgcg gcgtcgacct caccgtgaaa tccggtgaga cacatgcctt gatgggaccc aacggctcgg gcaagtcgac gctgtcctac gccatcgcgg gccatcccaa ataccacgtg acgtcgggca ccattaccct cgacggcgcg gacgtgctgg cgatgagcat cgacgaacgt gcgcgggccg gcctgtttct ggccatgcaa tatcccgtcg aggtgcccgg tgtctcgatg tcgaacttcc tgcgctcggc ggcaaccgcc attcgcggcg agccgccgaa actgcggcac tgggtcaaag aggtcaaggc cgcgatggcc gcgctcgaca tcgacccggc cttcgccgag cgcagcgtca acgagggttt ctccggtggc gagaagaagc gccacgagat cctgcagcta gaactgctca agcccaagat cgccatcctg gacgagaccg actccggcct ggacgtcgac gcgctgcgcg tggtcagcga gggggtgaac cgctacgccg aatcccagca cggcggcatc ctgctgatca cgcactacac ccgcatcctg cgctacatcc acccggaata cgtgcacgtg ttcgtcggcg gccgcatcgt cgagtccggt ggttcggagc tcgccgacga actcgaccag aacggctacg tgcgtttctc ccccgcaagc gggcggtacc cccaccaacc cgcgccaacc ggagccaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa
Details for MytuD.00236.a.A1.PW25865
PURIFICATION DATe: 2/24/2009
CONCENTRATION: 27.82mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 10
VIAL VOLUME: 100µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTILEIKDL HVSVENPAEA DHEIPILRGV DLTVKSGETH ALMGPNGSGK STLSYAIAGH PKYHVTSGTI TLDGADVLAM SIDERARAGL FLAMQYPVEV PGVSMSNFLR SAATAIRGEP PKLRHWVKEV KAAMAALDID PAFAERSVNE GFSGGEKKRH EILQLELLKP KIAILDETDS GLDVDALRVV SEGVNRYAES QHGGILLITH YTRILRYIHP EYVHVFVGGR IVESGGSELA DELDQNGYVR FSPASGRYPH QPAPTGA
Validated NT Sequence
tttaagaagg agatatacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatgaccat tttggaaatt aaggacctgc acgtcagcgt ggagaacccc gcggaggcgg accacgagat cccgatcctg cgcggcgtcg acctcaccgt gaaatccggt gagacacatg ccttgatggg acccaacggc tcgggcaagt cgacgctgtc ctacgccatc gcgggccatc ccaaatacca cgtgacgtcg ggcaccatta ccctcgacgg cgcggacgtg ctggcgatga gcatcgacga acgtgcgcgg gccggcctgt ttctggccat gcaatatccc gtcgaggtgc ccggtgtctc gatgtcgaac ttcctgcgct cggcggcaac cgccattcgc ggcgagccgc cgaaactgcg gcactgggtc aaagaggtca aggccgcgat ggccgcgctc gacatcgacc cggccttcgc cgagcgcagc gtcaacgagg gtttctccgg tggcgagaag aagcgccacg agatcctgca gctagaactg ctcaagccca agatcgccat cctggacgag accgactccg gcctggacgt cgacgcgctg cgcgtggtca gcgagggggt gaaccgctac gccgaatccc agcacggcgg catcctgctg atcacgcact acacccgcat cctgcgctac atccacccgg aatacgtgca cgtgttcgtc ggcggccgca tcgtcgagtc cggtggttcg gagctcgccg acgaactcga ccagaacggc tacgtgcgtt tctcccccgc aagcgggcgg tacccccacc aacccgcgcc aaccggagcc taataa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTILEIKDL HVSVENPAEA DHEIPILRGV DLTVKSGETH ALMGPNGSGK STLSYAIAGH PKYHVTSGTI TLDGADVLAM SIDERARAGL FLAMQYPVEV PGVSMSNFLR SAATAIRGEP PKLRHWVKEV KAAMAALDID PAFAERSVNE GFSGGEKKRH EILQLELLKP KIAILDETDS GLDVDALRVV SEGVNRYAES QHGGILLITH YTRILRYIHP EYVHVFVGGR IVESGGSELA DELDQNGYVR FSPASGRYPH QPAPTGA
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GACCATTTTG GAAATTAAGG ACCTGCACGT CAGCGTGGAG AACCCCGCGG AGGCGGACCA CGAGATCCCG ATCCTGCGCG GCGTCGACCT CACCGTGAAA TCCGGTGAGA CACATGCCTT GATGGGACCC AACGGCTCGG GCAAGTCGAC GCTGTCCTAC GCCATCGCGG GCCATCCCAA ATACCACGTG ACGTCGGGCA CCATTACCCT CGACGGCGCG GACGTGCTGG CGATGAGCAT CGACGAACGT GCGCGGGCCG GCCTGTTTCT GGCCATGCAA TATCCCGTCG AGGTGCCCGG TGTCTCGATG TCGAACTTCC TGCGCTCGGC GGCAACCGCC ATTCGCGGCG AGCCGCCGAA ACTGCGGCAC TGGGTCAAAG AGGTCAAGGC CGCGATGGCC GCGCTCGACA TCGACCCGGC CTTCGCCGAG CGCAGCGTCA ACGAGGGTTT CTCCGGTGGC GAGAAGAAGC GCCACGAGAT CCTGCAGCTA GAACTGCTCA AGCCCAAGAT CGCCATCCTG GACGAGACCG ACTCCGGCCT GGACGTCGAC GCGCTGCGCG TGGTCAGCGA GGGGGTGAAC CGCTACGCCG AATCCCAGCA CGGCGGCATC CTGCTGATCA CGCACTACAC CCGCATCCTG CGCTACATCC ACCCGGAATA CGTGCACGTG TTCGTCGGCG GCCGCATCGT CGAGTCCGGT GGTTCGGAGC TCGCCGACGA ACTCGACCAG AACGGCTACG TGCGTTTCTC CCCCGCAAGC GGGCGGTACC CCCACCAACC CGCGCCAACC GGAGCCAAAC AGCACGAACA AGTTCTGCAG CCAAGCTTCT CGAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAA