MyulA.00236.a

Conserved ATP-binding protein ABC transporter

CENTER ID: MyulA.00236.a
ORGANISM: Mycobacterium ulcerans Agy99
ASSOCIATED DISEASE: Buruli, Bairnsdale ulcer
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyulA.00236.a.A1.GE29662 Full length( MyulA.00236.a ) 1 260
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|362242.7.peg.2224
RefSeq: YP_905801.1
UniProt: A0PPR1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTTLIIKDLH VSVENPNAAE GEREIPILKG VDLTVKSGET HALMGSNGSG KSTLSYAIAG HPKYQVTSGS ITLDGADVLE MSVDERARAG LFLAMQYPVE VPGVSVSNFL RSAATAIRGE APKLRHWVKE VKAAMAALEI DPVFAERSVN EGFSGGEKKR HEILQLELLK PKIAILDETD SGLDVDALRV VSEGINRYAE AEHGGILLIT HYTRILRYIR PEFVHVFVGG RIAESGGPEL ADELEQNGYV RFTQAAAAGA
NT Sequence
ATGACCACAT TGATTATCAA GGACCTGCAC GTCAGCGTCG AGAATCCCAA CGCCGCCGAG GGCGAGCGCG AGATTCCCAT CCTCAAGGGC GTCGATCTGA CCGTGAAATC CGGTGAGACA CACGCTTTGA TGGGCTCCAA CGGCTCCGGC AAGTCCACGT TGTCCTACGC CATCGCCGGT CACCCCAAGT ACCAGGTGAC ATCGGGTTCC ATCACGCTAG ACGGCGCCGA CGTGCTGGAG ATGAGTGTCG ACGAGCGCGC CCGAGCCGGT CTGTTTCTGG CCATGCAGTA CCCCGTCGAG GTGCCCGGTG TCTCGGTGTC GAACTTCCTG CGTTCGGCCG CGACGGCCAT CCGCGGCGAG GCGCCGAAGC TGCGGCACTG GGTCAAAGAG GTCAAGGCCG CGATGGCCGC CCTCGAGATC GACCCCGTGT TCGCCGAGCG CAGCGTCAAC GAGGGCTTTT CCGGCGGCGA GAAGAAGCGC CATGAGATTC TGCAGCTGGA ACTACTCAAG CCCAAGATCG CCATTCTCGA CGAGACCGAC TCCGGCCTCG ACGTCGACGC GTTGCGCGTG GTCAGCGAGG GGATAAACCG CTACGCGGAG GCAGAACACG GCGGCATCTT GTTGATCACC CACTACACCC GCATTCTGCG TTACATCCGT CCAGAGTTTG TCCACGTGTT CGTCGGCGGC CGCATCGCCG AGTCCGGTGG GCCCGAGCTG GCCGACGAAC TTGAGCAGAA CGGGTACGTC CGCTTTACCC AAGCGGCGGC CGCAGGGGCC
Details for MyulA.00236.a.A1.GE29662
HARVESTED ON: 6/22/2010
SEQUENCED ON: 7/1/2010
EXPECTED MW: 30kDa
OBSERVED MW: 30kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 90
PERCENT COVERAGE: 112
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTTLIIKDL HVSVENPNAA EGEREIPILK GVDLTVKSGE THALMGSNGS GKSTLSYAIA GHPKYQVTSG SITLDGADVL EMSVDERARA GLFLAMQYPV EVPGVSVSNF LRSAATAIRG EAPKLRHWVK EVKAAMAALE IDPVFAERSV NEGFSGGEKK RHEILQLELL KPKIAILDET DSGLDVDALR VVSEGINRYA XAEHGGILLI THYTRILRYI RPEFVHVFVG XPHRXXRWAR AXPXNLSRTG TSALPKRRXX GPXNSTNXXC SXXSXXXAXX AXXXLXXXCX XXXXXXX
Validated NT Sequence
attttgttta actttaagaa ggagatatac catggctcat caccatcacc atcatatggg taccctggaa gctcagaccc agggtcctgg ttcgatgacc acattgatta tcaaggacct gcacgtcagc gtcgagaatc ccaacgccgc cgagggcgag cgcgagattc ccatcctcaa gggcgtcgat ctgaccgtga aatccggtga gacacacgct ttgatgggct ccaacggctc cggcaagtcc acgttgtcct acgccatcgc cggtcacccc aagtaccagg tgacatcggg ttccatcacg ctagacggcg ccgacgtgct ggagatgagt gtcgacgagc gcgcccgagc cggtctgttt ctggccatgc agtaccccgt cgaggtgccc ggtgtctcgg tgtcgaactt cctgcgttcg gccgcgacgg ccatccgcgg cgaggcgccg aagctgcggc actgggtcaa agaggtcaag gccgcgatgg ccgccctcga gatcgacccc gtgttcgccg agcgcagcgt caacgagggc ttttccggcg gcgagaagaa gcgccatgag attctgcagc tggaactact caagcccaag atcgccattc tcgacgagac cgactccggc ctcgacgtcg acgcgttgcg cgtggtcagc gaggggataa accgctacgc ggangcagaa cacggcggca tcttgttgat cacccactac acccgcattc tgcgttacat ccgtccagag tttgtccacg tgttcgtcgg cnggccgcat cgccnantcc ggtgggcccg agctnggccg angaacttga gcagaacggg tacgtccgct ttacccaagc ggcggcngcn gggncctana aacagcacga acnagtnctg cagcnagntn tcnagnnccn ntgctancna agccngaang nagctnantn gntgntgcnn nnnnnnnnnn nnnnnnnnnn nnaaa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTTLIIKDL HVSVENPNAA EGEREIPILK GVDLTVKSGE THALMGSNGS GKSTLSYAIA GHPKYQVTSG SITLDGADVL EMSVDERARA GLFLAMQYPV EVPGVSVSNF LRSAATAIRG EAPKLRHWVK EVKAAMAALE IDPVFAERSV NEGFSGGEKK RHEILQLELL KPKIAILDET DSGLDVDALR VVSEGINRYA EAEHGGILLI THYTRILRYI RPEFVHVFVG GRIAESGGPE LADELEQNGY VRFTQAAAAG A
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaccacattg attatcaagg acctgcacgt cagcgtcgag aatcccaacg ccgccgaggg cgagcgcgag attcccatcc tcaagggcgt cgatctgacc gtgaaatccg gtgagacaca cgctttgatg ggctccaacg gctccggcaa gtccacgttg tcctacgcca tcgccggtca ccccaagtac caggtgacat cgggttccat cacgctagac ggcgccgacg tgctggagat gagtgtcgac gagcgcgccc gagccggtct gtttctggcc atgcagtacc ccgtcgaggt gcccggtgtc tcggtgtcga acttcctgcg ttcggccgcg acggccatcc gcggcgaggc gccgaagctg cggcactggg tcaaagaggt caaggccgcg atggccgccc tcgagatcga ccccgtgttc gccgagcgca gcgtcaacga gggcttttcc ggcggcgaga agaagcgcca tgagattctg cagctggaac tactcaagcc caagatcgcc attctcgacg agaccgactc cggcctcgac gtcgacgcgt tgcgcgtggt cagcgagggg ataaaccgct acgcggaggc agaacacggc ggcatcttgt tgatcaccca ctacacccgc attctgcgtt acatccgtcc agagtttgtc cacgtgttcg tcggcggccg catcgccgag tccggtgggc ccgagctggc cgacgaactt gagcagaacg ggtacgtccg ctttacccaa gcggcggccg caggggccaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa