MypaA.00236.a

Putative uncharacterized protein

CENTER ID: MypaA.00236.a
ORGANISM: Mycobacterium paratuberculosis ATCC BAA-968 / K-10
ASSOCIATED DISEASE: Johne's disease
CURRENT STATUS: crystallized
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MypaA.00236.a.A1.GE29189 Full length( MypaA.00236.a ) 1 260
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MypaA.00236.a.A1.PS00724 Full length( MypaA.00236.a ) 1 260

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|262316.17.peg.1250
RefSeq: NP_960123.1
UniProt: Q741A3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTTLEIKDLH VSVVNPNAAE SEREIPILNG VDLTVKSGET HALMGPNGSG KSTLSYAIAG HPKYTVTSGS ITLDGQDVLA MSIDERARAG IFLAMQYPVE VPGVSVSNFL RSAATAVRGE APKLRHWVKE VKAAMGALEI DPAFAERNVN EGFSGGEKKR HEILQLELLK PKIAILDETD SGLDVDALRV VSEGVNRYAQ AEHGGILLIT HYTRILRYIH PEYVHVFVGG RIAESGGSEL ADELEQNGYV RFTQAAAAGA
NT Sequence
ATGACCACTC TGGAAATCAA AGACCTGCAC GTCAGCGTCG TGAACCCGAA CGCCGCCGAG TCCGAGCGCG AGATTCCCAT CCTCAACGGG GTCGACCTGA CGGTGAAATC CGGTGAGACG CATGCCTTGA TGGGCCCCAA CGGCTCGGGC AAGTCGACGC TGTCCTACGC CATCGCCGGT CACCCCAAGT ACACGGTGAC CTCCGGCTCC ATCACCCTGG ACGGCCAGGA CGTGCTGGCG ATGAGCATCG ACGAGCGCGC CCGCGCCGGC ATCTTTCTGG CCATGCAGTA CCCCGTCGAG GTGCCCGGCG TCTCGGTGTC GAATTTCCTG CGCTCGGCGG CGACCGCGGT GCGCGGCGAG GCCCCCAAAC TGCGGCACTG GGTCAAAGAG GTCAAGGCGG CCATGGGTGC CCTCGAGATC GACCCCGCGT TCGCCGAGCG CAACGTCAAC GAGGGCTTCT CCGGCGGGGA GAAGAAGCGC CACGAGATCC TGCAGCTGGA GCTGCTCAAG CCCAAGATCG CCATCCTCGA CGAGACCGAC TCGGGGCTCG ACGTCGACGC GCTGCGGGTG GTCAGCGAGG GCGTCAACCG CTACGCGCAG GCCGAGCATG GCGGCATCCT GCTGATCACC CACTACACCC GCATCCTGCG CTACATCCAC CCCGAATACG TGCACGTCTT CGTCGGCGGC CGGATCGCCG AATCCGGTGG CTCGGAGCTG GCCGACGAAC TCGAGCAGAA CGGCTACGTG CGCTTCACCC AAGCGGCGGC AGCGGGGGCG
Details for MypaA.00236.a.A1.GE29189
HARVESTED ON: 4/27/2010
SEQUENCED ON: 5/1/2010
EXPECTED MW: 30kDa
OBSERVED MW: 30kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTTLEIKDL HVSVVNPNAA ESEREIPILN GVDLTVKSGE THALMGPNGS GKSTLSYAIA GHPKYTVTSG SITLDGQDVL AMSIDERARA GIFLAMQYPV EVPGVSVSNF LRSAATAVRG EAPKLRHWVK EVKAAMGALE IDPAFAERNV NEGFSGGEKK RHEILQLELL KPKIAILDET DSGLDVDALR VVSEGVNRYA QAEHGGILLI THYTRILRYI HPEYVHVFVG GRIAESGGSE LADELEQNGY VRFTQAAAAG A
Validated NT Sequence
ttattacgcc cccgctgccg ccgcttgggt gaagcgcacg tagccgttct gctcgagttc gtcggccagc tccgagccac cggattcggc gatccggccg ccgacgaaga cgtgcacgta ttcggggtgg atgtagcgca ggatgcgggt gtagtgggtg atcagcagga tgccgccatg ctcggcctgc gcgtagcggt tgacgccctc gctgaccacc cgcagcgcgt cgacgtcgag ccccgagtcg gtctcgtcga ggatggcgat cttgggcttg agcagctcca gctgcaggat ctcgtggcgc ttcttctccc cgccggagaa gccctcgttg acgttgcgct cggcgaacgc ggggtcgatc tcgagggcac ccatggccgc cttgacctct ttgacccagt gccgcagttt gggggcctcg ccgcgcaccg cggtcgccgc cgagcgcagg aaattcgaca ccgagacgcc gggcacctcg acggggtact gcatggccag aaagatgccg gcgcgggcgc gctcgtcgat gctcatcgcc agcacgtcct ggccgtccag ggtgatggag ccggaggtca ccgtgtactt ggggtgaccg gcgatggcgt aggacagcgt cgacttgccc gagccgttgg ggcccatcaa ggcatgcgtc tcaccggatt tcaccgtcag gtcgaccccg ttgaggatgg gaatctcgcg ctcggactcg gcggcgttcg ggttcacgac gctgacgtgc aggtctttga tttccagagt ggtcatcgaa ccaggaccct gggtctgagc ttccagggta cccatatgat ggtgatggtg atgagccat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTTLEIKDL HVSVVNPNAA ESEREIPILN GVDLTVKSGE THALMGPNGS GKSTLSYAIA GHPKYTVTSG SITLDGQDVL AMSIDERARA GIFLAMQYPV EVPGVSVSNF LRSAATAVRG EAPKLRHWVK EVKAAMGALE IDPAFAERNV NEGFSGGEKK RHEILQLELL KPKIAILDET DSGLDVDALR VVSEGVNRYA QAEHGGILLI THYTRILRYI HPEYVHVFVG GRIAESGGSE LADELEQNGY VRFTQAAAAG A
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaccactctg gaaatcaaag acctgcacgt cagcgtcgtg aacccgaacg ccgccgagtc cgagcgcgag attcccatcc tcaacggggt cgacctgacg gtgaaatccg gtgagacgca tgccttgatg ggccccaacg gctcgggcaa gtcgacgctg tcctacgcca tcgccggtca ccccaagtac acggtgacct ccggctccat caccctggac ggccaggacg tgctggcgat gagcatcgac gagcgcgccc gcgccggcat ctttctggcc atgcagtacc ccgtcgaggt gcccggcgtc tcggtgtcga atttcctgcg ctcggcggcg accgcggtgc gcggcgaggc ccccaaactg cggcactggg tcaaagaggt caaggcggcc atgggtgccc tcgagatcga ccccgcgttc gccgagcgca acgtcaacga gggcttctcc ggcggggaga agaagcgcca cgagatcctg cagctggagc tgctcaagcc caagatcgcc atcctcgacg agaccgactc ggggctcgac gtcgacgcgc tgcgggtggt cagcgagggc gtcaaccgct acgcgcaggc cgagcatggc ggcatcctgc tgatcaccca ctacacccgc atcctgcgct acatccaccc cgaatacgtg cacgtcttcg tcggcggccg gatcgccgaa tccggtggct cggagctggc cgacgaactc gagcagaacg gctacgtgcg cttcacccaa gcggcggcag cgggggcgaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa
Details for MypaA.00236.a.A1.PS00724
PURIFICATION DATe: 8/12/2010
CONCENTRATION: 36.9mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 3
VIAL VOLUME: data unavailable
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTTLEIKDL HVSVVNPNAA ESEREIPILN GVDLTVKSGE THALMGPNGS GKSTLSYAIA GHPKYTVTSG SITLDGQDVL AMSIDERARA GIFLAMQYPV EVPGVSVSNF LRSAATAVRG EAPKLRHWVK EVKAAMGALE IDPAFAERNV NEGFSGGEKK RHEILQLELL KPKIAILDET DSGLDVDALR VVSEGVNRYA QAEHGGILLI THYTRILRYI HPEYVHVFVG GRIAESGGSE LADELEQNGY VRFTQAAAAG A
Validated NT Sequence
ttattacgcc cccgctgccg ccgcttgggt gaagcgcacg tagccgttct gctcgagttc gtcggccagc tccgagccac cggattcggc gatccggccg ccgacgaaga cgtgcacgta ttcggggtgg atgtagcgca ggatgcgggt gtagtgggtg atcagcagga tgccgccatg ctcggcctgc gcgtagcggt tgacgccctc gctgaccacc cgcagcgcgt cgacgtcgag ccccgagtcg gtctcgtcga ggatggcgat cttgggcttg agcagctcca gctgcaggat ctcgtggcgc ttcttctccc cgccggagaa gccctcgttg acgttgcgct cggcgaacgc ggggtcgatc tcgagggcac ccatggccgc cttgacctct ttgacccagt gccgcagttt gggggcctcg ccgcgcaccg cggtcgccgc cgagcgcagg aaattcgaca ccgagacgcc gggcacctcg acggggtact gcatggccag aaagatgccg gcgcgggcgc gctcgtcgat gctcatcgcc agcacgtcct ggccgtccag ggtgatggag ccggaggtca ccgtgtactt ggggtgaccg gcgatggcgt aggacagcgt cgacttgccc gagccgttgg ggcccatcaa ggcatgcgtc tcaccggatt tcaccgtcag gtcgaccccg ttgaggatgg gaatctcgcg ctcggactcg gcggcgttcg ggttcacgac gctgacgtgc aggtctttga tttccagagt ggtcatcgaa ccaggaccct gggtctgagc ttccagggta cccatatgat ggtgatggtg atgagccat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTTLEIKDL HVSVVNPNAA ESEREIPILN GVDLTVKSGE THALMGPNGS GKSTLSYAIA GHPKYTVTSG SITLDGQDVL AMSIDERARA GIFLAMQYPV EVPGVSVSNF LRSAATAVRG EAPKLRHWVK EVKAAMGALE IDPAFAERNV NEGFSGGEKK RHEILQLELL KPKIAILDET DSGLDVDALR VVSEGVNRYA QAEHGGILLI THYTRILRYI HPEYVHVFVG GRIAESGGSE LADELEQNGY VRFTQAAAAG A
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GACCACTCTG GAAATCAAAG ACCTGCACGT CAGCGTCGTG AACCCGAACG CCGCCGAGTC CGAGCGCGAG ATTCCCATCC TCAACGGGGT CGACCTGACG GTGAAATCCG GTGAGACGCA TGCCTTGATG GGCCCCAACG GCTCGGGCAA GTCGACGCTG TCCTACGCCA TCGCCGGTCA CCCCAAGTAC ACGGTGACCT CCGGCTCCAT CACCCTGGAC GGCCAGGACG TGCTGGCGAT GAGCATCGAC GAGCGCGCCC GCGCCGGCAT CTTTCTGGCC ATGCAGTACC CCGTCGAGGT GCCCGGCGTC TCGGTGTCGA ATTTCCTGCG CTCGGCGGCG ACCGCGGTGC GCGGCGAGGC CCCCAAACTG CGGCACTGGG TCAAAGAGGT CAAGGCGGCC ATGGGTGCCC TCGAGATCGA CCCCGCGTTC GCCGAGCGCA ACGTCAACGA GGGCTTCTCC GGCGGGGAGA AGAAGCGCCA CGAGATCCTG CAGCTGGAGC TGCTCAAGCC CAAGATCGCC ATCCTCGACG AGACCGACTC GGGGCTCGAC GTCGACGCGC TGCGGGTGGT CAGCGAGGGC GTCAACCGCT ACGCGCAGGC CGAGCATGGC GGCATCCTGC TGATCACCCA CTACACCCGC ATCCTGCGCT ACATCCACCC CGAATACGTG CACGTCTTCG TCGGCGGCCG GATCGCCGAA TCCGGTGGCT CGGAGCTGGC CGACGAACTC GAGCAGAACG GCTACGTGCG CTTCACCCAA GCGGCGGCAG CGGGGGCGAA ACAGCACGAA CAAGTTCTGC AGCCAAGCTT CTCGAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA