SSGCID: Target Page

MyleA.00236.a

Putative ABC transporter ATP-binding protein

CENTER ID:	MyleA.00236.a
ORGANISM:	Mycobacterium leprae Br4923
ASSOCIATED DISEASE:	Hansen's disease, leprosy
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyleA.00236.a.A1.GE34931	Full length( MyleA.00236.a )		1	260

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|561304.4.peg.1032
RefSeq:	YP_002503135.1
UniProt:	A0A0H3MQ12

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTTLIVKDLY VSVEHPNAAE SECQIPILNG VDLTVKSGET HALMGPNGSG KSTLSYAIMG HPKYRVISGA ITLDGADVLT MSVDERARAG LFLAMQYPVE VPGVSVSNFL RSAATVIRGE PPKLRHWVKE VKAAMGALEI DPVFAERSVN EGFSGGEKKR HEILQLELLK PKIAILDETD SGLDVDALRV VSDGVNRYAD SEHGGILLIT HYTRILRYIR PEFVHVFVGG RIVESGGSEL ADELEQNGYV RFSQPAATGV

NT Sequence

ATGACGACTT TGATAGTCAA AGACTTGTAC GTAAGCGTTG AGCATCCCAA CGCCGCTGAA TCCGAGTGCC AGATCCCCAT CCTCAATGGC GTCGACCTGA CCGTGAAATC TGGGGAAACG CATGCGTTGA TGGGCCCCAA CGGATCCGGC AAGTCCACGT TGTCTTATGC TATCATGGGC CATCCCAAGT ACCGGGTTAT CTCGGGTGCC ATCACACTAG ACGGCGCGGA CGTGCTAACG ATGAGCGTTG ACGAGCGCGC GCGAGCCGGG TTGTTTCTAG CCATGCAGTA TCCCGTTGAG GTTCCTGGAG TATCGGTGTC GAACTTTCTG CGCTCGGCCG CGACCGTCAT TCGCGGCGAA CCGCCTAAAC TACGGCACTG GGTCAAAGAG GTGAAAGCCG CGATGGGTGC GCTGGAGATC GATCCGGTTT TCGCCGAACG CAGTGTCAAC GAGGGCTTCT CCGGTGGCGA AAAGAAGCGC CATGAGATCC TGCAACTGGA ACTGCTCAAG CCTAAAATCG CCATCTTAGA CGAGACCGAT TCCGGGTTGG ATGTCGACGC GTTGCGTGTG GTTAGCGATG GGGTGAACCG CTACGCTGAT TCGGAGCACG GCGGCATTTT GTTGATCACC CACTATACGC GCATCCTGCG CTACATTCGT CCGGAATTCG TACACGTGTT TGTCGGCGGC CGGATCGTCG AATCTGGTGG CTCGGAGCTG GCCGACGAAC TTGAGCAGAA CGGCTACGTA CGTTTCTCCC AACCGGCGGC AACCGGAGTC

Details for MyleA.00236.a.A1.GE34931

HARVESTED ON:	4/17/2012
SEQUENCED ON:	4/17/2012
EXPECTED MW:	30kDa
OBSERVED MW:	30kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMTTLIVKDL YVSVEHPNAA ESECQIPILN GVDLTVKSGE THALMGPNGS GKSTLSYAIM GHPKYRVISG AITLDGADVL TMSVDERARA GLFLAMQYPV EVPGVSVSNF LRSAATVIRG EPPKLRHWVK EVKAAMGALE IDPVFAERSV NEGFSGGEKK RHEILQLELL KPKIAILDET DSGLDVDALR VVSDGVNRYA DSEHGGILLI THYTRILRYI RPEFVHVFVG GRIVESGGSE LXDELEQNGY VRFSQPAATG X

Validated NT Sequence

annnnttnnt tnnctttaag aaggaganat accatggctc atcaccatca ccatcatatg ggtaccctgg aagctcagac ccagggtcct ggttcgatga cgactttgat agtcaaagac ttgtacgtaa gcgttgagca tcccaacgcc gctgaatccg agtgccagat ccccatcctc aatggcgtcg acctgaccgt gaaatctggg gaaacgcatg cgttgatggg ccccaacgga tccggcaagt ccacgttgtc ttatgctatc atgggccatc ccaagtaccg ggttatctcg ggtgccatca cactagacgg cgcggacgtg ctaacgatga gcgttgacga gcgcgcgcga gccgggttgt ttctagccat gcagtatccc gttgaggttc ctggagtatc ggtgtcgaac tttctgcgct cggccgcgac cgtcattcgc ggcgaaccgc ctaaactacg gcactgggtc aaagaggtga aagccgcgat gggtgcgctg gagatcgatc cggttttcgc cgaacgcagt gtcaacgagg gcttctccgg tggcgaaaag aagcgccatg agatcctgca actggaactg ctcaagccta aaatcgccat cttagacgag accgattccg ggttggatgt cgacgcgttg cgtgtggtta gcgatggggt gaaccgctac gctgattcgg agcacggcgg cattttgttg atcacccact atacgcgcat cctgcgctac attcgtccgg aattcgtaca cgtgtttgtc ggcggccgga tcgtcgaatc tggtggctcg gagctggncg acgaacttga gcagaacggc tacgtacgtt tctcccaacc ggcggcaacc ggantctaat aaacagcacg aacnagttct gcagccaagc ttctcgnnnn nnnnnnnncc ccnnnnnnnn nnnnnnnnnn naannnnnnn nnnaannnnn nanncnnc

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gacgactttg atagtcaaag acttgtacgt aagcgttgag catcccaacg ccgctgaatc cgagtgccag atccccatcc tcaatggcgt cgacctgacc gtgaaatctg gggaaacgca tgcgttgatg ggccccaacg gatccggcaa gtccacgttg tcttatgcta tcatgggcca tcccaagtac cgggttatct cgggtgccat cacactagac ggcgcggacg tgctaacgat gagcgttgac gagcgcgcgc gagccgggtt gtttctagcc atgcagtatc ccgttgaggt tcctggagta tcggtgtcga actttctgcg ctcggccgcg accgtcattc gcggcgaacc gcctaaacta cggcactggg tcaaagaggt gaaagccgcg atgggtgcgc tggagatcga tccggttttc gccgaacgca gtgtcaacga gggcttctcc ggtggcgaaa agaagcgcca tgagatcctg caactggaac tgctcaagcc taaaatcgcc atcttagacg agaccgattc cgggttggat gtcgacgcgt tgcgtgtggt tagcgatggg gtgaaccgct acgctgattc ggagcacggc ggcattttgt tgatcaccca ctatacgcgc atcctgcgct acattcgtcc ggaattcgta cacgtgtttg tcggcggccg gatcgtcgaa tctggtggct cggagctggc cgacgaactt gagcagaacg gctacgtacg tttctcccaa ccggcggcaa ccggagtcaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa