MytuD.00213.a

Enoyl-CoA hydratase

CENTER ID: MytuD.00213.a
ORGANISM: Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE: Tuberculosis
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MytuD.00213.a.A1.GE25815 Full length( MytuD.00213.a ) 1 274
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|83332.12.peg.4205
RefSeq: NP_218291.1
TubercuList: Rv3774
MTB Network Portal: Rv3774
UniProt: P75019

Orthologues for Rv3774

Species % Identity % Coverage Clones Proteins Structures
marinum 90 100 1 1 0
leprae 88 100 0 0 0
avium 84 100 1 1 0
paratuberculosis 84 100 1 1 0
smegmatis 80 100 2 1 1
abscessus 68 98 3 2 0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MGETYESVTV ETKDQVAQVT LIGPGKGNAM GPAFWSEMPE VFHALDADRE VRAIVITGSG KNFSYGLDVP AMGGMFAPLI ADGALARPRT DFHTEILRMQ KAINAVADCR TPTIAAVQGW CIGGAVDLIS AVDIRYASAD AKFSVREVKL AIVADMGSLA RLPLILSDGH LRELALTGKN IDAARAEKIG LVNDVYDDAD QTLAAAHATA AEIAANPPLA VYGIKDVLDQ QRTSAVSENL RYVAAWNAAF LPSKDLTEGI SATFAKRPPQ FTGE
NT Sequence
ATGGGCGAAA CCTACGAATC CGTCACCGTC GAAACCAAGG ACCAGGTCGC GCAGGTGACG CTGATCGGGC CGGGCAAGGG CAACGCGATG GGGCCCGCAT TCTGGTCGGA GATGCCCGAG GTGTTCCATG CCCTGGACGC CGACCGTGAG GTGCGGGCCA TCGTCATCAC CGGATCGGGC AAGAACTTCA GCTACGGCCT GGACGTACCG GCCATGGGCG GAATGTTCGC CCCGTTGATC GCCGACGGCG CGCTGGCCCG CCCACGCACG GACTTCCACA CCGAAATACT GCGCATGCAG AAGGCGATCA ACGCCGTCGC CGACTGCCGC ACCCCCACGA TCGCGGCCGT CCAGGGTTGG TGCATCGGCG GCGCCGTCGA CCTGATCTCC GCGGTCGACA TCCGGTATGC CAGCGCCGAC GCGAAGTTCT CGGTGCGCGA GGTCAAGCTA GCGATTGTTG CCGACATGGG CAGCCTGGCG CGCCTTCCAC TAATCCTGAG CGACGGCCAT CTACGAGAAC TCGCGCTGAC CGGCAAAAAT ATCGACGCGG CCCGCGCCGA GAAGATCGGC CTGGTCAACG ACGTCTACGA TGACGCCGAC CAGACGCTGG CCGCGGCCCA CGCGACTGCC GCCGAGATCG CCGCCAACCC ACCTTTGGCG GTCTACGGCA TCAAGGACGT TCTCGACCAA CAACGCACGT CCGCCGTCTC GGAGAACCTG CGCTATGTCG CCGCCTGGAA CGCCGCGTTT CTGCCGTCCA AGGACCTCAC CGAAGGTATT TCCGCGACGT TCGCCAAGCG CCCGCCCCAG TTCACCGGCG AG
Details for MytuD.00213.a.A1.GE25815
HARVESTED ON: 11/19/2008
SEQUENCED ON: 8/18/2010
EXPECTED MW: 31kDa
OBSERVED MW: 32kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMGETYESVT VETKDQVAQV TLIGPGKGNA MGPAFWSEMP EVFHALDADR EVRAIVITGS GKNFSYGLDV PAMGGMFAPL IADGALARPR TDFHTEILRM QKAINAVADC RTPTIAAVQG WCIGGAVDLI SAVDIRYASA DAKFSVREVK LAIVADMGSL ARLPLILSDG HLRELALTGK NIDAARAEKI GLVNDVYDDA DQTLAAAHAT AAEIAANPPL AVYGIKDVLD QQRTSAVSEN LRYVAAWNAA FLPSKDLTEG ISATFAKRPP QFTGE
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgggcg aaacctacga atccgtcacc gtcgaaacca aggaccaggt cgcgcaggtg acgctgatcg ggccgggcaa gggcaacgcg atggggcccg cattctggtc ggagatgccc gaggtgttcc atgccctgga cgccgaccgt gaggtgcggg ccatcgtcat caccggatcg ggcaagaact tcagctacgg cctggacgta ccggccatgg gcggaatgtt cgccccgttg atcgccgacg gcgcgctggc ccgcccacgc acggacttcc acaccgaaat actgcgcatg cagaaggcga tcaacgccgt cgccgactgc cgcaccccca cgatcgcggc cgtccagggt tggtgcatcg gcggcgccgt cgacctgatc tccgcggtcg acatccggta tgccagcgcc gacgcgaagt tctcggtgcg cgaggtcaag ctagcgattg ttgccgacat gggcagcctg gcgcgccttc cactaatcct gagcgacggc catctacgag aactcgcgct gaccggcaaa aatatcgacg cggcccgcgc cgagaagatc ggcctggtca acgacgtcta cgatgacgcc gaccagacgc tggccgcggc ccacgcgact gccgccgaga tcgccgccaa cccacctttg gcggtctacg gcatcaagga cgttctcgac caacaacgca cgtccgccgt ctcggagaac ctgcgctatg tcgccgcctg gaacgccgcg tttctgccgt ccaaggacct caccgaaggt atttccgcga cgttcgccaa gcgcccgccc cagttcaccg gcgagtaata a
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMGETYESVT VETKDQVAQV TLIGPGKGNA MGPAFWSEMP EVFHALDADR EVRAIVITGS GKNFSYGLDV PAMGGMFAPL IADGALARPR TDFHTEILRM QKAINAVADC RTPTIAAVQG WCIGGAVDLI SAVDIRYASA DAKFSVREVK LAIVADMGSL ARLPLILSDG HLRELALTGK NIDAARAEKI GLVNDVYDDA DQTLAAAHAT AAEIAANPPL AVYGIKDVLD QQRTSAVSEN LRYVAAWNAA FLPSKDLTEG ISATFAKRPP QFTGE
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gggcgaaacc tacgaatccg tcaccgtcga aaccaaggac caggtcgcgc aggtgacgct gatcgggccg ggcaagggca acgcgatggg gcccgcattc tggtcggaga tgcccgaggt gttccatgcc ctggacgccg accgtgaggt gcgggccatc gtcatcaccg gatcgggcaa gaacttcagc tacggcctgg acgtaccggc catgggcgga atgttcgccc cgttgatcgc cgacggcgcg ctggcccgcc cacgcacgga cttccacacc gaaatactgc gcatgcagaa ggcgatcaac gccgtcgccg actgccgcac ccccacgatc gcggccgtcc agggttggtg catcggcggc gccgtcgacc tgatctccgc ggtcgacatc cggtatgcca gcgccgacgc gaagttctcg gtgcgcgagg tcaagctagc gattgttgcc gacatgggca gcctggcgcg ccttccacta atcctgagcg acggccatct acgagaactc gcgctgaccg gcaaaaatat cgacgcggcc cgcgccgaga agatcggcct ggtcaacgac gtctacgatg acgccgacca gacgctggcc gcggcccacg cgactgccgc cgagatcgcc gccaacccac ctttggcggt ctacggcatc aaggacgttc tcgaccaaca acgcacgtcc gccgtctcgg agaacctgcg ctatgtcgcc gcctggaacg ccgcgtttct gccgtccaag gacctcaccg aaggtatttc cgcgacgttc gccaagcgcc cgccccagtt caccggcgag aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa