MymaA.00213.a

Enoyl-CoA hydratase, EchA21

CENTER ID: MymaA.00213.a
ORGANISM: Mycobacterium marinum ATCC BAA-535 / M
ASSOCIATED DISEASE: Cutaneous ulcerations, deep abscesses
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MymaA.00213.a.A1.GE29961 Full length( MymaA.00213.a ) 1 274
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MymaA.00213.a.A1.PW29837 Full length( MymaA.00213.a ) 1 274

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216594.6.peg.5713
RefSeq: YP_001853588.1
UniProt: B2HLK1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MGETYESVTV EIKDQVAQVT LIGPGKGNAM GPAFWSELPE VFAALDADRD VRAIVITGSG KNFSYGLDVP AMGGTFAPLM AEGALARPRT DFHAEVLRMQ KATNAVADCR TPTIASVHGW CIGGGVDLIS AVDMRYASAD AKFSIREVKL AIVADMGSLA RLPMILNDGH LRELALTGRD IDAARAEKIG LVNQVFEDAD ATLAAAHATA TEIAANPPLA VYGVKDVLDQ QRASAVAENL RYVAAWNAAF LPSKDLTEGI SATFAKRPPQ FTGE
NT Sequence
ATGGGCGAAA CATACGAATC TGTCACCGTC GAGATCAAAG ACCAGGTCGC ACAGGTGACG TTGATCGGGC CGGGCAAGGG TAATGCAATG GGACCCGCTT TCTGGTCGGA GCTGCCGGAA GTCTTCGCGG CGCTGGACGC CGACCGTGAT GTGCGGGCGA TCGTCATCAC CGGGTCGGGC AAGAACTTCA GTTACGGACT GGACGTGCCG GCGATGGGTG GAACTTTCGC CCCGCTGATG GCCGAAGGGG CGCTGGCGCG TCCCCGCACC GACTTTCATG CCGAGGTGCT GCGGATGCAG AAGGCGACCA ATGCGGTCGC CGATTGCCGG ACCCCGACCA TCGCGTCGGT GCACGGCTGG TGCATTGGCG GCGGCGTCGA CCTGATCTCG GCGGTGGACA TGCGCTACGC GAGTGCGGAT GCGAAGTTCT CGATACGCGA GGTCAAGCTC GCCATCGTCG CCGACATGGG CAGCCTGGCC CGCCTTCCGA TGATCCTCAA CGACGGTCAT CTGCGGGAGC TGGCGCTGAC CGGTCGGGAT ATCGACGCCG CTCGGGCCGA GAAGATCGGC CTGGTCAACC AGGTGTTCGA AGACGCCGAC GCAACCTTGG CCGCCGCCCA CGCCACCGCC ACCGAGATTG CCGCCAATCC CCCGCTGGCG GTGTACGGCG TCAAAGACGT CCTCGACCAG CAGCGCGCGT CGGCCGTCGC GGAAAACCTG CGCTACGTCG CGGCCTGGAA TGCGGCGTTT CTGCCGTCCA AGGACCTCAC CGAGGGAATA TCGGCGACCT TCGCCAAGCG GCCTCCCCAG TTCACCGGGG AG
Details for MymaA.00213.a.A1.GE29961
HARVESTED ON: 7/13/2010
SEQUENCED ON: 8/18/2010
EXPECTED MW: 31kDa
OBSERVED MW: 32kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMGETYESVT VEIKDQVAQV TLIGPGKGNA MGPAFWSELP EVFAALDADR DVRAIVITGS GKNFSYGLDV PAMGGTFAPL MAEGALARPR TDFHAEVLRM QKATNAVADC RTPTIASVHG WCIGGGVDLI SAVDMRYASA DAKFSIREVK LAIVADMGSL ARLPMILNDG HLRELALTGR DIDAARAEKI GLVNQVFEDA DATLAAAHAT ATEIAANPPL AVYGVKDVLD QQRASAVAEN LRYVAAWNAA FLPSKDLTEG ISATFAKRPP QFTG
Validated NT Sequence
ttattacccg gtgaactggg gaggccgctt ggcgaaggtc gccgatattc cctcggtgag gtccttggac ggcagaaacg ccgcattcca ggccgcgacg tagcgcaggt tttccgcgac ggccgacgcg cgctgctggt cgaggacgtc tttgacgccg tacaccgcca gcgggggatt ggcggcaatc tcggtggcgg tggcgtgggc ggcggccaag gttgcgtcgg cgtcttcgaa cacctggttg accaggccga tcttctcggc ccgagcggcg tcgatatccc gaccggtcag cgccagctcc cgcagatgac cgtcgttgag gatcatcgga aggcgggcca ggctgcccat gtcggcgacg atggcgagct tgacctcgcg tatcgagaac ttcgcatccg cactcgcgta gcgcatgtcc accgccgaga tcaggtcgac gccgccgcca atgcaccagc cgtgcaccga cgcgatggtc ggggtccggc aatcggcgac cgcattggtc gccttctgca tccgcagcac ctcggcatga aagtcggtgc ggggacgcgc cagcgcccct tcggccatca gcggggcgaa agttccaccc atcgccggca cgtccagtcc gtaactgaag ttcttgcccg acccggtgat gacgatcgcc cgcacatcac ggtcggcgtc cagcgccgcg aagacttccg gcagctccga ccagaaagcg ggtcccattg cattaccctt gcccggcccg atcaacgtca cctgtgcgac ctggtctttg atctcgacgg tgacagattc gtatgtttcg cccatcgaac caggaccctg ggtctgagct tccagggtac ccatatgatg gtgatggtga tgagccatgg ta
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMGETYESVT VEIKDQVAQV TLIGPGKGNA MGPAFWSELP EVFAALDADR DVRAIVITGS GKNFSYGLDV PAMGGTFAPL MAEGALARPR TDFHAEVLRM QKATNAVADC RTPTIASVHG WCIGGGVDLI SAVDMRYASA DAKFSIREVK LAIVADMGSL ARLPMILNDG HLRELALTGR DIDAARAEKI GLVNQVFEDA DATLAAAHAT ATEIAANPPL AVYGVKDVLD QQRASAVAEN LRYVAAWNAA FLPSKDLTEG ISATFAKRPP QFTG
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gggcgaaaca tacgaatctg tcaccgtcga gatcaaagac caggtcgcac aggtgacgtt gatcgggccg ggcaagggta atgcaatggg acccgctttc tggtcggagc tgccggaagt cttcgcggcg ctggacgccg accgtgatgt gcgggcgatc gtcatcaccg ggtcgggcaa gaacttcagt tacggactgg acgtgccggc gatgggtgga actttcgccc cgctgatggc cgaaggggcg ctggcgcgtc cccgcaccga ctttcatgcc gaggtgctgc ggatgcagaa ggcgaccaat gcggtcgccg attgccggac cccgaccatc gcgtcggtgc acggctggtg cattggcggc ggcgtcgacc tgatctcggc ggtggacatg cgctacgcga gtgcggatgc gaagttctcg atacgcgagg tcaagctcgc catcgtcgcc gacatgggca gcctggcccg ccttccgatg atcctcaacg acggtcatct gcgggagctg gcgctgaccg gtcgggatat cgacgccgct cgggccgaga agatcggcct ggtcaaccag gtgttcgaag acgccgacgc aaccttggcc gccgcccacg ccaccgccac cgagattgcc gccaatcccc cgctggcggt gtacggcgtc aaagacgtcc tcgaccagca gcgcgcgtcg gccgtcgcgg aaaacctgcg ctacgtcgcg gcctggaatg cggcgtttct gccgtccaag gacctcaccg agggaatatc ggcgaccttc gccaagcggc ctccccagtt caccgggaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa
Details for MymaA.00213.a.A1.PW29837
PURIFICATION DATe: 11/8/2010
CONCENTRATION: 28.8mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 15
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMGETYESVT VEIKDQVAQV TLIGPGKGNA MGPAFWSELP EVFAALDADR DVRAIVITGS GKNFSYGLDV PAMGGTFAPL MAEGALARPR TDFHAEVLRM QKATNAVADC RTPTIASVHG WCIGGGVDLI SAVDMRYASA DAKFSIREVK LAIVADMGSL ARLPMILNDG HLRELALTGR DIDAARAEKI GLVNQVFEDA DATLAAAHAT ATEIAANPPL AVYGVKDVLD QQRASAVAEN LRYVAAWNAA FLPSKDLTEG ISATFAKRPP QFTG
Validated NT Sequence
ttattacccg gtgaactggg gaggccgctt ggcgaaggtc gccgatattc cctcggtgag gtccttggac ggcagaaacg ccgcattcca ggccgcgacg tagcgcaggt tttccgcgac ggccgacgcg cgctgctggt cgaggacgtc tttgacgccg tacaccgcca gcgggggatt ggcggcaatc tcggtggcgg tggcgtgggc ggcggccaag gttgcgtcgg cgtcttcgaa cacctggttg accaggccga tcttctcggc ccgagcggcg tcgatatccc gaccggtcag cgccagctcc cgcagatgac cgtcgttgag gatcatcgga aggcgggcca ggctgcccat gtcggcgacg atggcgagct tgacctcgcg tatcgagaac ttcgcatccg cactcgcgta gcgcatgtcc accgccgaga tcaggtcgac gccgccgcca atgcaccagc cgtgcaccga cgcgatggtc ggggtccggc aatcggcgac cgcattggtc gccttctgca tccgcagcac ctcggcatga aagtcggtgc ggggacgcgc cagcgcccct tcggccatca gcggggcgaa agttccaccc atcgccggca cgtccagtcc gtaactgaag ttcttgcccg acccggtgat gacgatcgcc cgcacatcac ggtcggcgtc cagcgccgcg aagacttccg gcagctccga ccagaaagcg ggtcccattg cattaccctt gcccggcccg atcaacgtca cctgtgcgac ctggtctttg atctcgacgg tgacagattc gtatgtttcg cccatcgaac caggaccctg ggtctgagct tccagggtac ccatatgatg gtgatggtga tgagccatgg ta
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMGETYESVT VEIKDQVAQV TLIGPGKGNA MGPAFWSELP EVFAALDADR DVRAIVITGS GKNFSYGLDV PAMGGTFAPL MAEGALARPR TDFHAEVLRM QKATNAVADC RTPTIASVHG WCIGGGVDLI SAVDMRYASA DAKFSIREVK LAIVADMGSL ARLPMILNDG HLRELALTGR DIDAARAEKI GLVNQVFEDA DATLAAAHAT ATEIAANPPL AVYGVKDVLD QQRASAVAEN LRYVAAWNAA FLPSKDLTEG ISATFAKRPP QFTG
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGGCGAAACA TACGAATCTG TCACCGTCGA GATCAAAGAC CAGGTCGCAC AGGTGACGTT GATCGGGCCG GGCAAGGGTA ATGCAATGGG ACCCGCTTTC TGGTCGGAGC TGCCGGAAGT CTTCGCGGCG CTGGACGCCG ACCGTGATGT GCGGGCGATC GTCATCACCG GGTCGGGCAA GAACTTCAGT TACGGACTGG ACGTGCCGGC GATGGGTGGA ACTTTCGCCC CGCTGATGGC CGAAGGGGCG CTGGCGCGTC CCCGCACCGA CTTTCATGCC GAGGTGCTGC GGATGCAGAA GGCGACCAAT GCGGTCGCCG ATTGCCGGAC CCCGACCATC GCGTCGGTGC ACGGCTGGTG CATTGGCGGC GGCGTCGACC TGATCTCGGC GGTGGACATG CGCTACGCGA GTGCGGATGC GAAGTTCTCG ATACGCGAGG TCAAGCTCGC CATCGTCGCC GACATGGGCA GCCTGGCCCG CCTTCCGATG ATCCTCAACG ACGGTCATCT GCGGGAGCTG GCGCTGACCG GTCGGGATAT CGACGCCGCT CGGGCCGAGA AGATCGGCCT GGTCAACCAG GTGTTCGAAG ACGCCGACGC AACCTTGGCC GCCGCCCACG CCACCGCCAC CGAGATTGCC GCCAATCCCC CGCTGGCGGT GTACGGCGTC AAAGACGTCC TCGACCAGCA GCGCGCGTCG GCCGTCGCGG AAAACCTGCG CTACGTCGCG GCCTGGAATG CGGCGTTTCT GCCGTCCAAG GACCTCACCG AGGGAATATC GGCGACCTTC GCCAAGCGGC CTCCCCAGTT CACCGGGAAA CAGCACGAAC AAGTTCTGCA GCCAAGCTTC TCGAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAA