MysmA.00213.a

Delta3,5-delta2,4-dienoyl-CoA isomerase (enoyl-CoA hydratase) (Ech)

CENTER ID: MysmA.00213.a
ORGANISM: Mycobacterium smegmatis ATCC 700084 / mc(2)155
ASSOCIATED DISEASE:
CURRENT STATUS: native diffraction-data
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:
IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
MysmA.00213.a.A1.GE28584 Full length( MysmA.00213.a ) 1 271
MysmA.00213.a.B1.GE43075 Full length( MysmA.00213.a ) 1 271
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
MysmA.00213.a.A1.PS00547 Full length( MysmA.00213.a ) 1 271
Structures
8SOY
DEPOSITED: 4/30/2023
DETERMINATION: CryoEM
CLONE: MysmA.00213.a.A1.GE28584
PROTEIN: MysmA.00213.a.A1.PS00547
External Resources
RESOURCE REFERENCE ID
OrthoMCL: OG5_129459
PATRIC ID: fig|246196.19.peg.6184
RefSeq: YP_890568.1
UniProt: A0R5Y0
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MADTYESVTV EVKDHVAQVT LIGPGKGNAM GPAFWEEMPD VFGTLDDDPD VRAIVLTGSG KNFSYGLDLP AMGASLPALD AGAKSRSDFH KRLQKMQGAI TAVADCRTPT IASVHGWCIG GGVDLISAVD IRYASADAKF SVREVKLAIV ADVGSLARLP LILSDGHLRE LALTGKDIDA ARAEKIGLVN DVYDDAEASL AAAHATAAEI AANPPLTVAG VKDVLDAQRT AQVAESLRYV AAWNSAFLPS KDLAEAVTAM FEKRRPEFTG E
NT Sequence
ATGGCTGACA CGTACGAGTC GGTGACCGTC GAGGTCAAAG ACCATGTCGC ACAGGTGACC CTGATCGGGC CCGGCAAGGG CAACGCGATG GGGCCCGCGT TCTGGGAAGA GATGCCCGAC GTGTTCGGCA CCCTCGACGA CGATCCCGAC GTACGCGCCA TCGTGCTGAC CGGCTCCGGC AAGAACTTCA GCTACGGCCT GGACCTGCCC GCCATGGGCG CCTCGCTGCC CGCCCTGGAC GCGGGCGCGA AGTCACGCTC CGACTTCCAC AAGCGCCTGC AGAAGATGCA GGGCGCCATC ACCGCGGTCG CCGACTGCCG CACGCCCACC ATCGCATCGG TGCACGGCTG GTGCATCGGC GGCGGCGTGG ACCTCATCAG CGCCGTGGAC ATCCGTTACG CGAGCGCCGA CGCCAAGTTC TCGGTGCGTG AGGTCAAGCT CGCGATCGTC GCCGACGTGG GCAGCCTCGC GCGTCTGCCG CTGATCCTCA GCGACGGACA TCTGCGCGAA TTGGCGCTCA CCGGCAAGGA CATCGACGCC GCACGGGCCG AGAAGATCGG TCTGGTCAAC GACGTCTACG ACGACGCCGA GGCCTCGCTG GCCGCCGCGC ACGCCACGGC CGCCGAGATC GCCGCCAACC CGCCGCTGAC GGTCGCAGGC GTCAAGGACG TGCTCGACGC GCAGCGCACC GCGCAGGTGG CCGAGAGCCT GCGGTACGTC GCGGCCTGGA ACTCGGCGTT CCTGCCGTCG AAGGACCTCG CCGAGGCGGT GACGGCGATG TTCGAAAAGC GCAGGCCCGA GTTCACCGGC GAG
Details for MysmA.00213.a.A1.GE28584
HARVESTED ON: 2/17/2010
SEQUENCED ON: 2/24/2010
EXPECTED MW: 30kDa
OBSERVED MW: 33kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Few (1-10)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 98
PERCENT COVERAGE: 94
Validated AA Sequence
MGTLEAQTQG PGSMADTYES VTVEVKDHVA QVTLIGPGKG NAMGPAFWEE MPDVFGTLDD DPDVRAIVLT GSGKNFSYGL DLPAMGASLP ALDAGAKSRS DFHKRLQKMQ GAITAVADCR TPTIASVHGW CIGGGVDLIS AVDIRYASAD AKFSVREVKL AIVADVGSLA RLPLILSDGH LRELALTGKD IDAARAEKIG LVNDVYDDAE ASLAAAHATA AEIAANPPLT VAGVKDVLDA QRTAQVXESL RYVAAWNSAF LPSXXLAEAV TAMFEKR
Validated NT Sequence
ccatcatatg ggtaccctgg aagctcagac ccagggtcct ggttcgatgg ctgacacgta cgagtcggtg accgtcgagg tcaaagacca tgtcgcacag gtgaccctga tcgggcccgg caagggcaac gcgatggggc ccgcgttctg ggaagagatg cccgacgtgt tcggcaccct cgacgacgat cccgacgtac gcgccatcgt gctgaccggc tccggcaaga acttcagcta cggcctggac ctgcccgcca tgggcgcctc gctgcccgcc ctggacgcgg gcgcgaagtc acgctccgac ttccacaagc gcctgcagaa gatgcagggc gccatcaccg cggtcgccga ctgccgcacg cccaccatcg catcggtgca cggctggtgc atcggcggcg gcgtggacct catcagcgcc gtggacatcc gttacgcgag cgccgacgcc aagttctcgg tgcgtgaggt caagctcgcg atcgtcgccg acgtgggcag cctcgcgcgt ctgccgctga tcctcagcga cggacatctg cgcgaattgg cgctcaccgg caaggacatc gacgccgcac gggccgagaa gatcggtctg gtcaacgacg tctacgacga cgccgaggcc tcgctggccg ccgcgcacgc cacggccgcc gagatcgccg ccaacccgcc gctgacggtc gcaggcgtca aggacgtgct cgacgcgcag cgcaccgcgc aggtggncga gagcctgcgg tacgtcgcgg cctggaactc ggcgttcctg ccgtcnaang anctcgccga ggcggtgacg gcgatgttcg aaaagcgca
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMADTYESVT VEVKDHVAQV TLIGPGKGNA MGPAFWEEMP DVFGTLDDDP DVRAIVLTGS GKNFSYGLDL PAMGASLPAL DAGAKSRSDF HKRLQKMQGA ITAVADCRTP TIASVHGWCI GGGVDLISAV DIRYASADAK FSVREVKLAI VADVGSLARL PLILSDGHLR ELALTGKDID AARAEKIGLV NDVYDDAEAS LAAAHATAAE IAANPPLTVA GVKDVLDAQR TAQVAESLRY VAAWNSAFLP SKDLAEAVTA MFEKRRPEFT GE
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggctgacacg tacgagtcgg tgaccgtcga ggtcaaagac catgtcgcac aggtgaccct gatcgggccc ggcaagggca acgcgatggg gcccgcgttc tgggaagaga tgcccgacgt gttcggcacc ctcgacgacg atcccgacgt acgcgccatc gtgctgaccg gctccggcaa gaacttcagc tacggcctgg acctgcccgc catgggcgcc tcgctgcccg ccctggacgc gggcgcgaag tcacgctccg acttccacaa gcgcctgcag aagatgcagg gcgccatcac cgcggtcgcc gactgccgca cgcccaccat cgcatcggtg cacggctggt gcatcggcgg cggcgtggac ctcatcagcg ccgtggacat ccgttacgcg agcgccgacg ccaagttctc ggtgcgtgag gtcaagctcg cgatcgtcgc cgacgtgggc agcctcgcgc gtctgccgct gatcctcagc gacggacatc tgcgcgaatt ggcgctcacc ggcaaggaca tcgacgccgc acgggccgag aagatcggtc tggtcaacga cgtctacgac gacgccgagg cctcgctggc cgccgcgcac gccacggccg ccgagatcgc cgccaacccg ccgctgacgg tcgcaggcgt caaggacgtg ctcgacgcgc agcgcaccgc gcaggtggcc gagagcctgc ggtacgtcgc ggcctggaac tcggcgttcc tgccgtcgaa ggacctcgcc gaggcggtga cggcgatgtt cgaaaagcgc aggcccgagt tcaccggcga gaaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa
Details for MysmA.00213.a.B1.GE43075
HARVESTED ON: 4/19/2019
SEQUENCED ON: 4/23/2019
EXPECTED MW: 30kDa
OBSERVED MW: 30kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 86
PERCENT COVERAGE: 86
Validated AA Sequence
MGXXXXXXXX XXFXXXXXXX DVRAIVLXXX GKNFSYGLDL XXXXXSLPAL DAGAKSRSXX HKRLQKMQGA ITAVADCRTP TIASVHGWCI GGGVDLISAV DIRYASADAK FSVREVKLAI VADVGSLARL PLILSDGHLR ELALTGKDID AARAEKIGLV NDVYDDAEAS LAAAHATAAE IAANPPLTVA GVKDVLDAQR TAQVAESLRY VAAWNSAFLP SKDLAEAVTA MFXXXXXEFT GE
Validated NT Sequence
ttactcactt actcgccggt gaactcnnnn nnnnnnnnnt cgaacatcgc cgtcaccgcc tcggcgaggt ccttcgacgg caggaacgcc gagttccagg ccgcgacgta ccgcaggctc tcggccacct gcgcggtgcg ctgcgcgtcg agcacgtcct tgacgcctgc gaccgtcagc ggcgggttgg cggcgatctc ggcggccgtg gcgtgcgcgg cggccagcga ggcctcggcg tcgtcgtaga cgtcgttgac cagaccgatc ttctcggccc gtgcggcgtc gatgtccttg ccggtgagcg ccaattcgcg cagatgtccg tcgctgagga tcagcggcag acgcgcgagg ctgcccacgt cggcgacgat cgcgagcttg acctcacgca ccgagaactt ggcgtcggcg ctcgcgtaac ggatgtccac ggcgctgatg aggtccacgc cgccgccgat gcaccagccg tgcaccgatg cgatggtggg cgtgcggcag tcggcgaccg cggtgatggc gccctgcatc ttctgcaggc gcttgtggan nncggagcgt gacttcgcgc ccgcgtccag ggcgggcagc gaggnnnnna tgnnnnnnag gtccaggccg tagctgaagt tcttgccggn nnnngnnagc acgatggcgc gtacgtcnng ntnnnnnnnn nnnnnnnnga annnnnnnnn cnnnnnnnnn nnnnnnnnnn nccccatnnn nnngc
Expected Protein Sequence
MAHHHHHHMA DTYESVTVEV KDHVAQVTLI GPGKGNAMGP AFWEEMPDVF GTLDDDPDVR AIVLTGSGKN FSYGLDLPAM GASLPALDAG AKSRSDFHKR LQKMQGAITA VADCRTPTIA SVHGWCIGGG VDLISAVDIR YASADAKFSV REVKLAIVAD VGSLARLPLI LSDGHLRELA LTGKDIDAAR AEKIGLVNDV YDDAEASLAA AHATAAEIAA NPPLTVAGVK DVLDAQRTAQ VAESLRYVAA WNSAFLPSKD LAEAVTAMFE KRRPEFTGE
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggctg acacgtacga gtcggtgacc gtcgaggtca aagaccatgt cgcacaggtg accctgatcg ggcccggcaa gggcaacgcg atggggcccg cgttctggga agagatgccc gacgtgttcg gcaccctcga cgacgatccc gacgtacgcg ccatcgtgct gaccggctcc ggcaagaact tcagctacgg cctggacctg cccgccatgg gcgcctcgct gcccgccctg gacgcgggcg cgaagtcacg ctccgacttc cacaagcgcc tgcagaagat gcagggcgcc atcaccgcgg tcgccgactg ccgcacgccc accatcgcat cggtgcacgg ctggtgcatc ggcggcggcg tggacctcat cagcgccgtg gacatccgtt acgcgagcgc cgacgccaag ttctcggtgc gtgaggtcaa gctcgcgatc gtcgccgacg tgggcagcct cgcgcgtctg ccgctgatcc tcagcgacgg acatctgcgc gaattggcgc tcaccggcaa ggacatcgac gccgcacggg ccgagaagat cggtctggtc aacgacgtct acgacgacgc cgaggcctcg ctggccgccg cgcacgccac ggccgccgag atcgccgcca acccgccgct gacggtcgca ggcgtcaagg acgtgctcga cgcgcagcgc accgcgcagg tggccgagag cctgcggtac gtcgcggcct ggaactcggc gttcctgccg tcgaaggacc tcgccgaggc ggtgacggcg atgttcgaaa agcgcaggcc cgagttcacc ggcgagtgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t
Details for MysmA.00213.a.A1.PS00547
PURIFICATION DATe: 4/9/2010
CONCENTRATION: 60.9mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 4
VIAL VOLUME: 200µl
PERCENT IDENTITY: 98
PERCENT COVERAGE: 94
Protocol Notes
notes unavailable
Validated AA Sequence
MGTLEAQTQG PGSMADTYES VTVEVKDHVA QVTLIGPGKG NAMGPAFWEE MPDVFGTLDD DPDVRAIVLT GSGKNFSYGL DLPAMGASLP ALDAGAKSRS DFHKRLQKMQ GAITAVADCR TPTIASVHGW CIGGGVDLIS AVDIRYASAD AKFSVREVKL AIVADVGSLA RLPLILSDGH LRELALTGKD IDAARAEKIG LVNDVYDDAE ASLAAAHATA AEIAANPPLT VAGVKDVLDA QRTAQVXESL RYVAAWNSAF LPSXXLAEAV TAMFEKR
Validated NT Sequence
ccatcatatg ggtaccctgg aagctcagac ccagggtcct ggttcgatgg ctgacacgta cgagtcggtg accgtcgagg tcaaagacca tgtcgcacag gtgaccctga tcgggcccgg caagggcaac gcgatggggc ccgcgttctg ggaagagatg cccgacgtgt tcggcaccct cgacgacgat cccgacgtac gcgccatcgt gctgaccggc tccggcaaga acttcagcta cggcctggac ctgcccgcca tgggcgcctc gctgcccgcc ctggacgcgg gcgcgaagtc acgctccgac ttccacaagc gcctgcagaa gatgcagggc gccatcaccg cggtcgccga ctgccgcacg cccaccatcg catcggtgca cggctggtgc atcggcggcg gcgtggacct catcagcgcc gtggacatcc gttacgcgag cgccgacgcc aagttctcgg tgcgtgaggt caagctcgcg atcgtcgccg acgtgggcag cctcgcgcgt ctgccgctga tcctcagcga cggacatctg cgcgaattgg cgctcaccgg caaggacatc gacgccgcac gggccgagaa gatcggtctg gtcaacgacg tctacgacga cgccgaggcc tcgctggccg ccgcgcacgc cacggccgcc gagatcgccg ccaacccgcc gctgacggtc gcaggcgtca aggacgtgct cgacgcgcag cgcaccgcgc aggtggncga gagcctgcgg tacgtcgcgg cctggaactc ggcgttcctg ccgtcnaang anctcgccga ggcggtgacg gcgatgttcg aaaagcgca
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMADTYESVT VEVKDHVAQV TLIGPGKGNA MGPAFWEEMP DVFGTLDDDP DVRAIVLTGS GKNFSYGLDL PAMGASLPAL DAGAKSRSDF HKRLQKMQGA ITAVADCRTP TIASVHGWCI GGGVDLISAV DIRYASADAK FSVREVKLAI VADVGSLARL PLILSDGHLR ELALTGKDID AARAEKIGLV NDVYDDAEAS LAAAHATAAE IAANPPLTVA GVKDVLDAQR TAQVAESLRY VAAWNSAFLP SKDLAEAVTA MFEKRRPEFT GE
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGCTGACACG TACGAGTCGG TGACCGTCGA GGTCAAAGAC CATGTCGCAC AGGTGACCCT GATCGGGCCC GGCAAGGGCA ACGCGATGGG GCCCGCGTTC TGGGAAGAGA TGCCCGACGT GTTCGGCACC CTCGACGACG ATCCCGACGT ACGCGCCATC GTGCTGACCG GCTCCGGCAA GAACTTCAGC TACGGCCTGG ACCTGCCCGC CATGGGCGCC TCGCTGCCCG CCCTGGACGC GGGCGCGAAG TCACGCTCCG ACTTCCACAA GCGCCTGCAG AAGATGCAGG GCGCCATCAC CGCGGTCGCC GACTGCCGCA CGCCCACCAT CGCATCGGTG CACGGCTGGT GCATCGGCGG CGGCGTGGAC CTCATCAGCG CCGTGGACAT CCGTTACGCG AGCGCCGACG CCAAGTTCTC GGTGCGTGAG GTCAAGCTCG CGATCGTCGC CGACGTGGGC AGCCTCGCGC GTCTGCCGCT GATCCTCAGC GACGGACATC TGCGCGAATT GGCGCTCACC GGCAAGGACA TCGACGCCGC ACGGGCCGAG AAGATCGGTC TGGTCAACGA CGTCTACGAC GACGCCGAGG CCTCGCTGGC CGCCGCGCAC GCCACGGCCG CCGAGATCGC CGCCAACCCG CCGCTGACGG TCGCAGGCGT CAAGGACGTG CTCGACGCGC AGCGCACCGC GCAGGTGGCC GAGAGCCTGC GGTACGTCGC GGCCTGGAAC TCGGCGTTCC TGCCGTCGAA GGACCTCGCC GAGGCGGTGA CGGCGATGTT CGAAAAGCGC AGGCCCGAGT TCACCGGCGA GAAACAGCAC GAACAAGTTC TGCAGCCAAG CTTCTCGAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAA