MypaA.00213.a

EchA21 Enoyl-CoA hydratase/isomerase

CENTER ID: MypaA.00213.a
ORGANISM: Mycobacterium paratuberculosis ATCC BAA-968 / K-10
ASSOCIATED DISEASE: Johne's disease
CURRENT STATUS: diffraction
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MypaA.00213.a.A1.GE29188 Full length( MypaA.00213.a ) 1 274
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MypaA.00213.a.A1.PS00716 Full length( MypaA.00213.a ) 1 274

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|262316.17.peg.263
RefSeq: NP_959183.1
UniProt: Q745P5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAQAYESVTV EKKGHVAQVT LIGPGKGNAM GPAFWSELPE LFETLDADPE VRAIVLTGSG KNFSYGLDVP AMGGSFTPLL SGDALAGPRA VFHREVKRMQ GAITAVADCR TPTIASVYGW CIGGGVDLIS AVDIRYASAD AKFSVREVKL AIVADVGSLA RLPYILNDGH LRELALTGKD IDAARAEKIG LVNDVYADAD ACLAAAHATA AEIAANPPLT VHGIKDVLDQ QRVSAVAESL RYVAAWNAAF LPSKDLTEGI AATFEKRPAQ FTGE
NT Sequence
ATGGCGCAAG CATACGAATC GGTCACCGTG GAGAAGAAGG GCCATGTCGC CCAGGTGACG CTGATCGGCC CGGGCAAGGG TAACGCGATG GGGCCGGCCT TCTGGTCGGA GCTGCCCGAG CTGTTCGAGA CGCTGGACGC CGACCCCGAG GTGCGGGCCA TCGTGCTGAC CGGTTCGGGC AAGAACTTCA GCTACGGGCT GGACGTGCCG GCGATGGGCG GATCGTTCAC CCCGCTGCTG TCGGGCGACG CGCTGGCCGG GCCGCGCGCC GTCTTCCACC GCGAGGTCAA GCGGATGCAG GGCGCGATCA CCGCCGTCGC CGACTGCCGC ACCCCCACCA TCGCGTCGGT GTACGGCTGG TGCATCGGCG GCGGCGTCGA CCTCATCTCG GCGGTCGACA TCCGCTACGC CAGCGCCGAC GCCAAGTTCT CGGTGCGCGA GGTCAAGCTG GCCATCGTCG CCGACGTCGG CAGCCTGGCC CGGCTGCCGT ACATCCTCAA CGACGGCCAC CTGCGCGAGC TGGCGCTGAC CGGCAAGGAC ATCGACGCCG CCCGCGCCGA GAAGATCGGG CTGGTCAACG ACGTGTACGC CGACGCCGAC GCGTGCCTGG CCGCCGCGCA TGCCACGGCC GCGGAGATCG CCGCCAACCC CCCGCTGACC GTGCACGGCA TCAAGGATGT CCTCGACCAG CAGCGCGTTT CGGCCGTCGC CGAGAGCCTG CGCTACGTGG CCGCCTGGAA CGCGGCCTTC CTGCCGTCCA AGGACCTCAC CGAGGGCATC GCGGCGACGT TCGAGAAGCG GCCGGCGCAG TTCACCGGCG AG
Details for MypaA.00213.a.A1.GE29188
HARVESTED ON: 4/27/2010
SEQUENCED ON: 5/1/2010
EXPECTED MW: 31kDa
OBSERVED MW: 31kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MGTLEAQTQG PGSMAQAYES VTVEKKGHVA QVTLIGPGKG NAMGPAFWSE LPELFETLDA DPEVRAIVLT GSGKNFSYGL DVPAMGGSFT PLLSGDALAG PRAVFHREVK RMQGAITAVA DCRTPTIASV YGWCIGGGVD LISAVDIRYA SADAKFSVRE VKLAIVADVG SLARLPYILN DGHLRELALT GKDIDAARAE KIGLVNDVYA DADACLAAAH ATAAEIAANP PLTVHGIKDV LDQQRVSAVA ESLRYVAAWN AAFLPSKDLT EGIAATFEKR PAQFTGE
Validated NT Sequence
ttattactcg ccggtgaact gcgccggccg cttctcgaac gtcgccgcga tgccctcggt gaggtccttg gacggcagga aggccgcgtt ccaggcggcc acgtagcgca ggctctcggc gacggccgaa acgcgctgct ggtcgaggac atccttgatg ccgtgcacgg tcagcggggg gttggcggcg atctccgcgg ccgtggcatg cgcggcggcc aggcacgcgt cggcgtcggc gtacacgtcg ttgaccagcc cgatcttctc ggcgcgggcg gcgtcgatgt ccttgccggt cagcgccagc tcgcgcaggt ggccgtcgtt gaggatgtac ggcagccggg ccaggctgcc gacgtcggcg acgatggcca gcttgacctc gcgcaccgag aacttggcgt cggcgctggc gtagcggatg tcgaccgccg agatgaggtc gacgccgccg ccgatgcacc agccgtacac cgacgcgatg gtgggggtgc ggcagtcggc gacggcggtg atcgcgccct gcatccgctt gacctcgcgg tggaagacgg cgcgcggccc ggccagcgcg tcgcccgaca gcagcggggt gaacgatccg cccatcgccg gcacgtccag cccgtagctg aagttcttgc ccgaaccggt cagcacgatg gcccgcacct cggggtcggc gtccagcgtc tcgaacagct cgggcagctc cgaccagaag gccggcccca tcgcgttacc cttgcccggg ccgatcagcg tcacctgggc gacatggccc ttcttctcca cggtgaccga ttcgtatgct tgcgccatcg aaccaggacc ctgggtctga gcttccaggg tacccatatg atggtgat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAQAYESVT VEKKGHVAQV TLIGPGKGNA MGPAFWSELP ELFETLDADP EVRAIVLTGS GKNFSYGLDV PAMGGSFTPL LSGDALAGPR AVFHREVKRM QGAITAVADC RTPTIASVYG WCIGGGVDLI SAVDIRYASA DAKFSVREVK LAIVADVGSL ARLPYILNDG HLRELALTGK DIDAARAEKI GLVNDVYADA DACLAAAHAT AAEIAANPPL TVHGIKDVLD QQRVSAVAES LRYVAAWNAA FLPSKDLTEG IAATFEKRPA QFTGE
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcgcaagca tacgaatcgg tcaccgtgga gaagaagggc catgtcgccc aggtgacgct gatcggcccg ggcaagggta acgcgatggg gccggccttc tggtcggagc tgcccgagct gttcgagacg ctggacgccg accccgaggt gcgggccatc gtgctgaccg gttcgggcaa gaacttcagc tacgggctgg acgtgccggc gatgggcgga tcgttcaccc cgctgctgtc gggcgacgcg ctggccgggc cgcgcgccgt cttccaccgc gaggtcaagc ggatgcaggg cgcgatcacc gccgtcgccg actgccgcac ccccaccatc gcgtcggtgt acggctggtg catcggcggc ggcgtcgacc tcatctcggc ggtcgacatc cgctacgcca gcgccgacgc caagttctcg gtgcgcgagg tcaagctggc catcgtcgcc gacgtcggca gcctggcccg gctgccgtac atcctcaacg acggccacct gcgcgagctg gcgctgaccg gcaaggacat cgacgccgcc cgcgccgaga agatcgggct ggtcaacgac gtgtacgccg acgccgacgc gtgcctggcc gccgcgcatg ccacggccgc ggagatcgcc gccaaccccc cgctgaccgt gcacggcatc aaggatgtcc tcgaccagca gcgcgtttcg gccgtcgccg agagcctgcg ctacgtggcc gcctggaacg cggccttcct gccgtccaag gacctcaccg agggcatcgc ggcgacgttc gagaagcggc cggcgcagtt caccggcgag aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa
Details for MypaA.00213.a.A1.PS00716
PURIFICATION DATe: 8/3/2010
CONCENTRATION: 27.58mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 12
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Protocol Notes
notes unavailable
Validated AA Sequence
MGTLEAQTQG PGSMAQAYES VTVEKKGHVA QVTLIGPGKG NAMGPAFWSE LPELFETLDA DPEVRAIVLT GSGKNFSYGL DVPAMGGSFT PLLSGDALAG PRAVFHREVK RMQGAITAVA DCRTPTIASV YGWCIGGGVD LISAVDIRYA SADAKFSVRE VKLAIVADVG SLARLPYILN DGHLRELALT GKDIDAARAE KIGLVNDVYA DADACLAAAH ATAAEIAANP PLTVHGIKDV LDQQRVSAVA ESLRYVAAWN AAFLPSKDLT EGIAATFEKR PAQFTGE
Validated NT Sequence
ttattactcg ccggtgaact gcgccggccg cttctcgaac gtcgccgcga tgccctcggt gaggtccttg gacggcagga aggccgcgtt ccaggcggcc acgtagcgca ggctctcggc gacggccgaa acgcgctgct ggtcgaggac atccttgatg ccgtgcacgg tcagcggggg gttggcggcg atctccgcgg ccgtggcatg cgcggcggcc aggcacgcgt cggcgtcggc gtacacgtcg ttgaccagcc cgatcttctc ggcgcgggcg gcgtcgatgt ccttgccggt cagcgccagc tcgcgcaggt ggccgtcgtt gaggatgtac ggcagccggg ccaggctgcc gacgtcggcg acgatggcca gcttgacctc gcgcaccgag aacttggcgt cggcgctggc gtagcggatg tcgaccgccg agatgaggtc gacgccgccg ccgatgcacc agccgtacac cgacgcgatg gtgggggtgc ggcagtcggc gacggcggtg atcgcgccct gcatccgctt gacctcgcgg tggaagacgg cgcgcggccc ggccagcgcg tcgcccgaca gcagcggggt gaacgatccg cccatcgccg gcacgtccag cccgtagctg aagttcttgc ccgaaccggt cagcacgatg gcccgcacct cggggtcggc gtccagcgtc tcgaacagct cgggcagctc cgaccagaag gccggcccca tcgcgttacc cttgcccggg ccgatcagcg tcacctgggc gacatggccc ttcttctcca cggtgaccga ttcgtatgct tgcgccatcg aaccaggacc ctgggtctga gcttccaggg tacccatatg atggtgat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAQAYESVT VEKKGHVAQV TLIGPGKGNA MGPAFWSELP ELFETLDADP EVRAIVLTGS GKNFSYGLDV PAMGGSFTPL LSGDALAGPR AVFHREVKRM QGAITAVADC RTPTIASVYG WCIGGGVDLI SAVDIRYASA DAKFSVREVK LAIVADVGSL ARLPYILNDG HLRELALTGK DIDAARAEKI GLVNDVYADA DACLAAAHAT AAEIAANPPL TVHGIKDVLD QQRVSAVAES LRYVAAWNAA FLPSKDLTEG IAATFEKRPA QFTGE
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGCGCAAGCA TACGAATCGG TCACCGTGGA GAAGAAGGGC CATGTCGCCC AGGTGACGCT GATCGGCCCG GGCAAGGGTA ACGCGATGGG GCCGGCCTTC TGGTCGGAGC TGCCCGAGCT GTTCGAGACG CTGGACGCCG ACCCCGAGGT GCGGGCCATC GTGCTGACCG GTTCGGGCAA GAACTTCAGC TACGGGCTGG ACGTGCCGGC GATGGGCGGA TCGTTCACCC CGCTGCTGTC GGGCGACGCG CTGGCCGGGC CGCGCGCCGT CTTCCACCGC GAGGTCAAGC GGATGCAGGG CGCGATCACC GCCGTCGCCG ACTGCCGCAC CCCCACCATC GCGTCGGTGT ACGGCTGGTG CATCGGCGGC GGCGTCGACC TCATCTCGGC GGTCGACATC CGCTACGCCA GCGCCGACGC CAAGTTCTCG GTGCGCGAGG TCAAGCTGGC CATCGTCGCC GACGTCGGCA GCCTGGCCCG GCTGCCGTAC ATCCTCAACG ACGGCCACCT GCGCGAGCTG GCGCTGACCG GCAAGGACAT CGACGCCGCC CGCGCCGAGA AGATCGGGCT GGTCAACGAC GTGTACGCCG ACGCCGACGC GTGCCTGGCC GCCGCGCATG CCACGGCCGC GGAGATCGCC GCCAACCCCC CGCTGACCGT GCACGGCATC AAGGATGTCC TCGACCAGCA GCGCGTTTCG GCCGTCGCCG AGAGCCTGCG CTACGTGGCC GCCTGGAACG CGGCCTTCCT GCCGTCCAAG GACCTCACCG AGGGCATCGC GGCGACGTTC GAGAAGCGGC CGGCGCAGTT CACCGGCGAG AAACAGCACG AACAAGTTCT GCAGCCAAGC TTCTCGAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AA