MytuD.00145.a

aspartyl-tRNA synthetase

CENTER ID: MytuD.00145.a
ORGANISM: Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE: Tuberculosis
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MytuD.00145.a.A1.GE25249 Full length( MytuD.00145.a ) 1 596
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MytuD.00145.a.A1.PD00422 Full length( MytuD.00145.a ) 1 596
MytuD.00145.a.A1.PD00427 Full length( MytuD.00145.a ) 1 596
MytuD.00145.a.A1.PD00429 Full length( MytuD.00145.a ) 1 596
MytuD.00145.a.A1.PD00487 Full length( MytuD.00145.a ) 1 596
MytuD.00145.a.A1.PW27581 Full length( MytuD.00145.a ) 1 596
External Resources
RESOURCE REFERENCE ID
OrthoMCL: OG5_127662
PATRIC ID: fig|83332.12.peg.2880
RefSeq: NP_217088.1
TubercuList: Rv2572c
MTB Network Portal: Rv2572c
UniProt: P9WFW3
Orthologues for Rv2572c
Species % Identity % Coverage Clones Proteins Structures
bovis 99 99 0 0 0
marinum 90 100 1 0 0
ulcerans 89 100 0 0 0
avium 88 99 0 0 0
leprae 88 99 1 0 0
paratuberculosis 88 99 0 0 0
smegmatis 82 99 1 0 1
abscessus 81 100 0 0 0
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MFVLRSHAAG LLREGDAGQQ VTLAGWVARR RDHGGVIFID LRDASGIAQV VFRDPQDTEV LAQAHRLRAE FCVSVAGVVE IRPEGNANPE IATGEIEVNA TSLTVLGECA PLPFQLDEPA GEELRLKYRY LDLRRDDPAA AIRLRSRVNA AARAVLARHD FVEIETPTIT RSTPEGARDF LVPARLHPGS FYALPQSPQL FKQLLMVAGM ERYYQIARCY RDEDFRADRQ PEFTQLDMEM SFVDAEDIIA ISEEVLTELW ALIGYRIPTP IPRIGYAEAM RRFGTDKPDL RFGLELVECT DFFSDTTFRV FQAPYVGAVV MPGGASQPRR TLDGWQDWAK QRGHRGLAYV LVAEDGTLGG PVAKNLTEAE RTGLADHVGA KPGDCIFFSA GPVKSSRALL GAARVEIANR LGLIDPDAWA FVWVVDPPLF EPADEATAAG EVAVGSGAWT AVHHAFTAPK PEWEDRIESD TGSVLADAYD IVCNGHEIGG GSVRIHRRDI QERVFAVMGL DKAEAEEKFG FLLEAFMFGA PPHGGIAFGW DRTTALLAGM DSIREVIAFP KTGGGVDPLT DAPAPITAQQ RKESGIDAQP KRVQQA
NT Sequence
GTGTTTGTGC TGCGCAGCCA CGCCGCGGGT TTGCTACGGG AAGGCGACGC CGGGCAGCAG GTGACATTGG CCGGCTGGGT GGCTCGCCGC CGCGACCACG GCGGCGTGAT CTTCATCGAT CTGCGGGACG CTTCGGGCAT CGCACAGGTG GTGTTCCGCG ACCCCCAGGA CACTGAGGTG CTGGCGCAGG CTCACCGGCT GCGTGCGGAA TTCTGCGTAT CGGTGGCCGG CGTCGTCGAG ATCCGCCCGG AAGGCAACGC CAACCCGGAG ATCGCCACCG GCGAGATCGA GGTCAACGCC ACGTCGTTGA CCGTGCTGGG CGAATGTGCG CCGCTGCCGT TTCAGCTGGA CGAACCGGCG GGTGAGGAGT TGCGACTGAA GTACCGCTAC CTCGATCTGC GCCGCGACGA CCCCGCTGCG GCGATTCGAC TGCGTTCCCG GGTGAATGCC GCTGCGCGCG CGGTGCTGGC GCGTCACGAC TTCGTCGAGA TCGAGACGCC GACGATCACC CGCTCGACCC CGGAGGGAGC GCGCGACTTC CTGGTGCCGG CCCGACTGCA CCCCGGTTCG TTTTACGCCC TACCGCAGAG CCCGCAGCTG TTCAAGCAGC TGCTGATGGT GGCGGGGATG GAACGCTACT ACCAGATCGC CCGCTGCTAC CGCGACGAGG ACTTCCGCGC CGACCGCCAG CCCGAATTCA CCCAGCTCGA TATGGAGATG AGCTTCGTCG ACGCCGAGGA CATCATCGCG ATCTCCGAGG AAGTTCTGAC CGAGCTGTGG GCGCTGATAG GCTACCGGAT TCCGACGCCC ATCCCGCGGA TCGGCTATGC CGAAGCGATG CGCCGATTCG GTACGGACAA ACCCGACCTG CGGTTCGGGC TGGAGCTCGT CGAATGCACG GATTTCTTCT CCGACACCAC ATTTCGTGTC TTCCAGGCAC CGTATGTGGG CGCGGTGGTG ATGCCTGGCG GGGCGTCGCA GCCGCGGCGC ACGCTGGACG GCTGGCAGGA CTGGGCCAAG CAGCGCGGCC ACCGCGGACT GGCCTACGTG CTGGTCGCCG AAGACGGCAC GCTGGGCGGT CCGGTGGCCA AAAACCTGAC CGAGGCCGAG CGCACCGGAC TGGCCGACCA TGTCGGGGCC AAACCCGGCG ATTGCATCTT CTTCTCGGCC GGTCCGGTCA AATCGTCGCG GGCACTGCTG GGCGCGGCCC GCGTCGAGAT CGCCAACCGG CTGGGCCTGA TTGACCCCGA TGCGTGGGCA TTCGTCTGGG TCGTTGACCC GCCGCTGTTC GAGCCGGCCG ACGAAGCGAC CGCCGCCGGT GAGGTCGCGG TCGGCTCGGG GGCCTGGACC GCGGTGCACC ATGCCTTCAC CGCGCCGAAA CCGGAATGGG AGGACCGCAT CGAATCCGAT ACCGGCAGCG TGCTGGCCGA CGCGTACGAC ATCGTCTGCA ACGGCCACGA GATCGGTGGC GGCTCGGTGC GTATCCACCG CCGTGATATC CAGGAACGGG TGTTCGCGGT GATGGGCCTG GACAAGGCCG AGGCGGAGGA AAAGTTCGGA TTCCTGTTGG AGGCGTTCAT GTTTGGCGCA CCGCCGCACG GCGGAATCGC GTTCGGGTGG GACCGGACCA CCGCGCTGCT GGCCGGGATG GACTCGATCC GTGAGGTGAT CGCGTTCCCG AAGACCGGCG GCGGCGTCGA TCCGCTCACC GACGCGCCGG CGCCCATTAC CGCTCAGCAA CGTAAGGAGT CCGGAATAGA TGCCCAGCCC AAGCGGGTCC AGCAGGCA
Details for MytuD.00145.a.A1.GE25249
HARVESTED ON: 5/6/2008
SEQUENCED ON: 6/16/2009
EXPECTED MW: 67kDa
OBSERVED MW: 68kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 97
PERCENT COVERAGE: 51
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVFVLRSHA AGLLREGDAG QQVTLAGWVA RRRDHGGVIF IDLRDASGIA QVVFRDPQDT EVLAQAHRLR AEFCVSVAGV VEIRPEGNAN PEIATGEIEV NATSLTVLGE CAPLPFQLDE PAGEELRLKY RYLDLRRDDP AAAIRLRSRV NAAARAVLAR HDFVEIETPT ITRSTPEGAR DFLVPARLHP GSFYALPQSP QLFKQLLMVA GMERYYQIAR CYRDEDFRAD RQPEFTQLDM EMSFVDAXDI IAISEEVLXX LWALIGYRIP TPIPRIGYAX AMRRFGTXKP XLXFGLXL
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggtgt ttgtgctgcg cagccacgcc gcgggtttgc tacgggaagg cgacgccggg cagcaggtga cattggccgg ctgggtggct cgccgccgcg accacggcgg cgtgatcttc atcgatctgc gggacgcttc gggcatcgca caggtggtgt tccgcgaccc ccaggacact gaggtgctgg cgcaggctca ccggctgcgt gcggaattct gcgtatcggt ggccggcgtc gtcgagatcc gcccggaagg caacgccaac ccggagatcg ccaccggcga gatcgaggtc aacgccacgt cgttgaccgt gctgggcgaa tgtgcgccgc tgccgtttca gctggacgaa ccggcgggtg aggagttgcg actgaagtac cgctacctcg atctgcgccg cgacgacccc gctgcggcga ttcgactgcg ttcccgggtg aatgccgctg cgcgcgcggt gctggcgcgt cacgacttcg tcgagatcga gacgccgacg atcacccgct cgaccccgga gggagcgcgc gacttcctgg tgccggcccg actgcacccc ggttcgtttt acgccctacc gcagagcccg cagctgttca agcagctgct gatggtggcg gggatggaac gctactacca gatcgcccgc tgctaccgcg acgaggactt ccgcgccgac cgccagcccg aattcaccca gctcgatatg gagatgagct tcgtcgacgc cgangacatc atcgcgatct ccgaggaagt tctgancgan ctgtgggcgc tgataggcta ccggattccg acgcccatcc cgcggatcgg ctatgccnaa gcgatgcgcc gattcggtac gganaaaccn nacctgnggt tcgggctgga nctcgtc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVFVLRSHA AGLLREGDAG QQVTLAGWVA RRRDHGGVIF IDLRDASGIA QVVFRDPQDT EVLAQAHRLR AEFCVSVAGV VEIRPEGNAN PEIATGEIEV NATSLTVLGE CAPLPFQLDE PAGEELRLKY RYLDLRRDDP AAAIRLRSRV NAAARAVLAR HDFVEIETPT ITRSTPEGAR DFLVPARLHP GSFYALPQSP QLFKQLLMVA GMERYYQIAR CYRDEDFRAD RQPEFTQLDM EMSFVDAEDI IAISEEVLTE LWALIGYRIP TPIPRIGYAE AMRRFGTDKP DLRFGLELVE CTDFFSDTTF RVFQAPYVGA VVMPGGASQP RRTLDGWQDW AKQRGHRGLA YVLVAEDGTL GGPVAKNLTE AERTGLADHV GAKPGDCIFF SAGPVKSSRA LLGAARVEIA NRLGLIDPDA WAFVWVVDPP LFEPADEATA AGEVAVGSGA WTAVHHAFTA PKPEWEDRIE SDTGSVLADA YDIVCNGHEI GGGSVRIHRR DIQERVFAVM GLDKAEAEEK FGFLLEAFMF GAPPHGGIAF GWDRTTALLA GMDSIREVIA FPKTGGGVDP LTDAPAPITA QQRKESGIDA QPKRVQQA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgtttgtg ctgcgcagcc acgccgcggg tttgctacgg gaaggcgacg ccgggcagca ggtgacattg gccggctggg tggctcgccg ccgcgaccac ggcggcgtga tcttcatcga tctgcgggac gcttcgggca tcgcacaggt ggtgttccgc gacccccagg acactgaggt gctggcgcag gctcaccggc tgcgtgcgga attctgcgta tcggtggccg gcgtcgtcga gatccgcccg gaaggcaacg ccaacccgga gatcgccacc ggcgagatcg aggtcaacgc cacgtcgttg accgtgctgg gcgaatgtgc gccgctgccg tttcagctgg acgaaccggc gggtgaggag ttgcgactga agtaccgcta cctcgatctg cgccgcgacg accccgctgc ggcgattcga ctgcgttccc gggtgaatgc cgctgcgcgc gcggtgctgg cgcgtcacga cttcgtcgag atcgagacgc cgacgatcac ccgctcgacc ccggagggag cgcgcgactt cctggtgccg gcccgactgc accccggttc gttttacgcc ctaccgcaga gcccgcagct gttcaagcag ctgctgatgg tggcggggat ggaacgctac taccagatcg cccgctgcta ccgcgacgag gacttccgcg ccgaccgcca gcccgaattc acccagctcg atatggagat gagcttcgtc gacgccgagg acatcatcgc gatctccgag gaagttctga ccgagctgtg ggcgctgata ggctaccgga ttccgacgcc catcccgcgg atcggctatg ccgaagcgat gcgccgattc ggtacggaca aacccgacct gcggttcggg ctggagctcg tcgaatgcac ggatttcttc tccgacacca catttcgtgt cttccaggca ccgtatgtgg gcgcggtggt gatgcctggc ggggcgtcgc agccgcggcg cacgctggac ggctggcagg actgggccaa gcagcgcggc caccgcggac tggcctacgt gctggtcgcc gaagacggca cgctgggcgg tccggtggcc aaaaacctga ccgaggccga gcgcaccgga ctggccgacc atgtcggggc caaacccggc gattgcatct tcttctcggc cggtccggtc aaatcgtcgc gggcactgct gggcgcggcc cgcgtcgaga tcgccaaccg gctgggcctg attgaccccg atgcgtgggc attcgtctgg gtcgttgacc cgccgctgtt cgagccggcc gacgaagcga ccgccgccgg tgaggtcgcg gtcggctcgg gggcctggac cgcggtgcac catgccttca ccgcgccgaa accggaatgg gaggaccgca tcgaatccga taccggcagc gtgctggccg acgcgtacga catcgtctgc aacggccacg agatcggtgg cggctcggtg cgtatccacc gccgtgatat ccaggaacgg gtgttcgcgg tgatgggcct ggacaaggcc gaggcggagg aaaagttcgg attcctgttg gaggcgttca tgtttggcgc accgccgcac ggcggaatcg cgttcgggtg ggaccggacc accgcgctgc tggccgggat ggactcgatc cgtgaggtga tcgcgttccc gaagaccggc ggcggcgtcg atccgctcac cgacgcgccg gcgcccatta ccgctcagca acgtaaggag tccggaatag atgcccagcc caagcgggtc cagcaggcaa aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a
Details for MytuD.00145.a.A1.PD00422
PURIFICATION DATe: 2/9/2016
CONCENTRATION: 15.04mg/ml
OBSERVED MW: 68kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25mM Tris, pH 8.0, 200mM NaCl, 1% glycerol, 1mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 41
VIAL VOLUME: 200µl
PERCENT IDENTITY: 97
PERCENT COVERAGE: 51
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVFVLRSHA AGLLREGDAG QQVTLAGWVA RRRDHGGVIF IDLRDASGIA QVVFRDPQDT EVLAQAHRLR AEFCVSVAGV VEIRPEGNAN PEIATGEIEV NATSLTVLGE CAPLPFQLDE PAGEELRLKY RYLDLRRDDP AAAIRLRSRV NAAARAVLAR HDFVEIETPT ITRSTPEGAR DFLVPARLHP GSFYALPQSP QLFKQLLMVA GMERYYQIAR CYRDEDFRAD RQPEFTQLDM EMSFVDAXDI IAISEEVLXX LWALIGYRIP TPIPRIGYAX AMRRFGTXKP XLXFGLXL
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggtgt ttgtgctgcg cagccacgcc gcgggtttgc tacgggaagg cgacgccggg cagcaggtga cattggccgg ctgggtggct cgccgccgcg accacggcgg cgtgatcttc atcgatctgc gggacgcttc gggcatcgca caggtggtgt tccgcgaccc ccaggacact gaggtgctgg cgcaggctca ccggctgcgt gcggaattct gcgtatcggt ggccggcgtc gtcgagatcc gcccggaagg caacgccaac ccggagatcg ccaccggcga gatcgaggtc aacgccacgt cgttgaccgt gctgggcgaa tgtgcgccgc tgccgtttca gctggacgaa ccggcgggtg aggagttgcg actgaagtac cgctacctcg atctgcgccg cgacgacccc gctgcggcga ttcgactgcg ttcccgggtg aatgccgctg cgcgcgcggt gctggcgcgt cacgacttcg tcgagatcga gacgccgacg atcacccgct cgaccccgga gggagcgcgc gacttcctgg tgccggcccg actgcacccc ggttcgtttt acgccctacc gcagagcccg cagctgttca agcagctgct gatggtggcg gggatggaac gctactacca gatcgcccgc tgctaccgcg acgaggactt ccgcgccgac cgccagcccg aattcaccca gctcgatatg gagatgagct tcgtcgacgc cgangacatc atcgcgatct ccgaggaagt tctgancgan ctgtgggcgc tgataggcta ccggattccg acgcccatcc cgcggatcgg ctatgccnaa gcgatgcgcc gattcggtac gganaaaccn nacctgnggt tcgggctgga nctcgtc
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVFVLRSHA AGLLREGDAG QQVTLAGWVA RRRDHGGVIF IDLRDASGIA QVVFRDPQDT EVLAQAHRLR AEFCVSVAGV VEIRPEGNAN PEIATGEIEV NATSLTVLGE CAPLPFQLDE PAGEELRLKY RYLDLRRDDP AAAIRLRSRV NAAARAVLAR HDFVEIETPT ITRSTPEGAR DFLVPARLHP GSFYALPQSP QLFKQLLMVA GMERYYQIAR CYRDEDFRAD RQPEFTQLDM EMSFVDAEDI IAISEEVLTE LWALIGYRIP TPIPRIGYAE AMRRFGTDKP DLRFGLELVE CTDFFSDTTF RVFQAPYVGA VVMPGGASQP RRTLDGWQDW AKQRGHRGLA YVLVAEDGTL GGPVAKNLTE AERTGLADHV GAKPGDCIFF SAGPVKSSRA LLGAARVEIA NRLGLIDPDA WAFVWVVDPP LFEPADEATA AGEVAVGSGA WTAVHHAFTA PKPEWEDRIE SDTGSVLADA YDIVCNGHEI GGGSVRIHRR DIQERVFAVM GLDKAEAEEK FGFLLEAFMF GAPPHGGIAF GWDRTTALLA GMDSIREVIA FPKTGGGVDP LTDAPAPITA QQRKESGIDA QPKRVQQA
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGTTTGTG CTGCGCAGCC ACGCCGCGGG TTTGCTACGG GAAGGCGACG CCGGGCAGCA GGTGACATTG GCCGGCTGGG TGGCTCGCCG CCGCGACCAC GGCGGCGTGA TCTTCATCGA TCTGCGGGAC GCTTCGGGCA TCGCACAGGT GGTGTTCCGC GACCCCCAGG ACACTGAGGT GCTGGCGCAG GCTCACCGGC TGCGTGCGGA ATTCTGCGTA TCGGTGGCCG GCGTCGTCGA GATCCGCCCG GAAGGCAACG CCAACCCGGA GATCGCCACC GGCGAGATCG AGGTCAACGC CACGTCGTTG ACCGTGCTGG GCGAATGTGC GCCGCTGCCG TTTCAGCTGG ACGAACCGGC GGGTGAGGAG TTGCGACTGA AGTACCGCTA CCTCGATCTG CGCCGCGACG ACCCCGCTGC GGCGATTCGA CTGCGTTCCC GGGTGAATGC CGCTGCGCGC GCGGTGCTGG CGCGTCACGA CTTCGTCGAG ATCGAGACGC CGACGATCAC CCGCTCGACC CCGGAGGGAG CGCGCGACTT CCTGGTGCCG GCCCGACTGC ACCCCGGTTC GTTTTACGCC CTACCGCAGA GCCCGCAGCT GTTCAAGCAG CTGCTGATGG TGGCGGGGAT GGAACGCTAC TACCAGATCG CCCGCTGCTA CCGCGACGAG GACTTCCGCG CCGACCGCCA GCCCGAATTC ACCCAGCTCG ATATGGAGAT GAGCTTCGTC GACGCCGAGG ACATCATCGC GATCTCCGAG GAAGTTCTGA CCGAGCTGTG GGCGCTGATA GGCTACCGGA TTCCGACGCC CATCCCGCGG ATCGGCTATG CCGAAGCGAT GCGCCGATTC GGTACGGACA AACCCGACCT GCGGTTCGGG CTGGAGCTCG TCGAATGCAC GGATTTCTTC TCCGACACCA CATTTCGTGT CTTCCAGGCA CCGTATGTGG GCGCGGTGGT GATGCCTGGC GGGGCGTCGC AGCCGCGGCG CACGCTGGAC GGCTGGCAGG ACTGGGCCAA GCAGCGCGGC CACCGCGGAC TGGCCTACGT GCTGGTCGCC GAAGACGGCA CGCTGGGCGG TCCGGTGGCC AAAAACCTGA CCGAGGCCGA GCGCACCGGA CTGGCCGACC ATGTCGGGGC CAAACCCGGC GATTGCATCT TCTTCTCGGC CGGTCCGGTC AAATCGTCGC GGGCACTGCT GGGCGCGGCC CGCGTCGAGA TCGCCAACCG GCTGGGCCTG ATTGACCCCG ATGCGTGGGC ATTCGTCTGG GTCGTTGACC CGCCGCTGTT CGAGCCGGCC GACGAAGCGA CCGCCGCCGG TGAGGTCGCG GTCGGCTCGG GGGCCTGGAC CGCGGTGCAC CATGCCTTCA CCGCGCCGAA ACCGGAATGG GAGGACCGCA TCGAATCCGA TACCGGCAGC GTGCTGGCCG ACGCGTACGA CATCGTCTGC AACGGCCACG AGATCGGTGG CGGCTCGGTG CGTATCCACC GCCGTGATAT CCAGGAACGG GTGTTCGCGG TGATGGGCCT GGACAAGGCC GAGGCGGAGG AAAAGTTCGG ATTCCTGTTG GAGGCGTTCA TGTTTGGCGC ACCGCCGCAC GGCGGAATCG CGTTCGGGTG GGACCGGACC ACCGCGCTGC TGGCCGGGAT GGACTCGATC CGTGAGGTGA TCGCGTTCCC GAAGACCGGC GGCGGCGTCG ATCCGCTCAC CGACGCGCCG GCGCCCATTA CCGCTCAGCA ACGTAAGGAG TCCGGAATAG ATGCCCAGCC CAAGCGGGTC CAGCAGGCAA AACAGCACGA ACAAGTTCTG CAGCCAAGCT TCTCGAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA A
Details for MytuD.00145.a.A1.PD00427
PURIFICATION DATe: 3/2/2016
CONCENTRATION: 20.04mg/ml
OBSERVED MW: 68kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM Tris pH 8.0, 200 mM NaCl, 1% glycerol, 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 21
VIAL VOLUME: 200µl
PERCENT IDENTITY: 97
PERCENT COVERAGE: 51
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVFVLRSHA AGLLREGDAG QQVTLAGWVA RRRDHGGVIF IDLRDASGIA QVVFRDPQDT EVLAQAHRLR AEFCVSVAGV VEIRPEGNAN PEIATGEIEV NATSLTVLGE CAPLPFQLDE PAGEELRLKY RYLDLRRDDP AAAIRLRSRV NAAARAVLAR HDFVEIETPT ITRSTPEGAR DFLVPARLHP GSFYALPQSP QLFKQLLMVA GMERYYQIAR CYRDEDFRAD RQPEFTQLDM EMSFVDAXDI IAISEEVLXX LWALIGYRIP TPIPRIGYAX AMRRFGTXKP XLXFGLXL
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggtgt ttgtgctgcg cagccacgcc gcgggtttgc tacgggaagg cgacgccggg cagcaggtga cattggccgg ctgggtggct cgccgccgcg accacggcgg cgtgatcttc atcgatctgc gggacgcttc gggcatcgca caggtggtgt tccgcgaccc ccaggacact gaggtgctgg cgcaggctca ccggctgcgt gcggaattct gcgtatcggt ggccggcgtc gtcgagatcc gcccggaagg caacgccaac ccggagatcg ccaccggcga gatcgaggtc aacgccacgt cgttgaccgt gctgggcgaa tgtgcgccgc tgccgtttca gctggacgaa ccggcgggtg aggagttgcg actgaagtac cgctacctcg atctgcgccg cgacgacccc gctgcggcga ttcgactgcg ttcccgggtg aatgccgctg cgcgcgcggt gctggcgcgt cacgacttcg tcgagatcga gacgccgacg atcacccgct cgaccccgga gggagcgcgc gacttcctgg tgccggcccg actgcacccc ggttcgtttt acgccctacc gcagagcccg cagctgttca agcagctgct gatggtggcg gggatggaac gctactacca gatcgcccgc tgctaccgcg acgaggactt ccgcgccgac cgccagcccg aattcaccca gctcgatatg gagatgagct tcgtcgacgc cgangacatc atcgcgatct ccgaggaagt tctgancgan ctgtgggcgc tgataggcta ccggattccg acgcccatcc cgcggatcgg ctatgccnaa gcgatgcgcc gattcggtac gganaaaccn nacctgnggt tcgggctgga nctcgtc
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVFVLRSHA AGLLREGDAG QQVTLAGWVA RRRDHGGVIF IDLRDASGIA QVVFRDPQDT EVLAQAHRLR AEFCVSVAGV VEIRPEGNAN PEIATGEIEV NATSLTVLGE CAPLPFQLDE PAGEELRLKY RYLDLRRDDP AAAIRLRSRV NAAARAVLAR HDFVEIETPT ITRSTPEGAR DFLVPARLHP GSFYALPQSP QLFKQLLMVA GMERYYQIAR CYRDEDFRAD RQPEFTQLDM EMSFVDAEDI IAISEEVLTE LWALIGYRIP TPIPRIGYAE AMRRFGTDKP DLRFGLELVE CTDFFSDTTF RVFQAPYVGA VVMPGGASQP RRTLDGWQDW AKQRGHRGLA YVLVAEDGTL GGPVAKNLTE AERTGLADHV GAKPGDCIFF SAGPVKSSRA LLGAARVEIA NRLGLIDPDA WAFVWVVDPP LFEPADEATA AGEVAVGSGA WTAVHHAFTA PKPEWEDRIE SDTGSVLADA YDIVCNGHEI GGGSVRIHRR DIQERVFAVM GLDKAEAEEK FGFLLEAFMF GAPPHGGIAF GWDRTTALLA GMDSIREVIA FPKTGGGVDP LTDAPAPITA QQRKESGIDA QPKRVQQA
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGTTTGTG CTGCGCAGCC ACGCCGCGGG TTTGCTACGG GAAGGCGACG CCGGGCAGCA GGTGACATTG GCCGGCTGGG TGGCTCGCCG CCGCGACCAC GGCGGCGTGA TCTTCATCGA TCTGCGGGAC GCTTCGGGCA TCGCACAGGT GGTGTTCCGC GACCCCCAGG ACACTGAGGT GCTGGCGCAG GCTCACCGGC TGCGTGCGGA ATTCTGCGTA TCGGTGGCCG GCGTCGTCGA GATCCGCCCG GAAGGCAACG CCAACCCGGA GATCGCCACC GGCGAGATCG AGGTCAACGC CACGTCGTTG ACCGTGCTGG GCGAATGTGC GCCGCTGCCG TTTCAGCTGG ACGAACCGGC GGGTGAGGAG TTGCGACTGA AGTACCGCTA CCTCGATCTG CGCCGCGACG ACCCCGCTGC GGCGATTCGA CTGCGTTCCC GGGTGAATGC CGCTGCGCGC GCGGTGCTGG CGCGTCACGA CTTCGTCGAG ATCGAGACGC CGACGATCAC CCGCTCGACC CCGGAGGGAG CGCGCGACTT CCTGGTGCCG GCCCGACTGC ACCCCGGTTC GTTTTACGCC CTACCGCAGA GCCCGCAGCT GTTCAAGCAG CTGCTGATGG TGGCGGGGAT GGAACGCTAC TACCAGATCG CCCGCTGCTA CCGCGACGAG GACTTCCGCG CCGACCGCCA GCCCGAATTC ACCCAGCTCG ATATGGAGAT GAGCTTCGTC GACGCCGAGG ACATCATCGC GATCTCCGAG GAAGTTCTGA CCGAGCTGTG GGCGCTGATA GGCTACCGGA TTCCGACGCC CATCCCGCGG ATCGGCTATG CCGAAGCGAT GCGCCGATTC GGTACGGACA AACCCGACCT GCGGTTCGGG CTGGAGCTCG TCGAATGCAC GGATTTCTTC TCCGACACCA CATTTCGTGT CTTCCAGGCA CCGTATGTGG GCGCGGTGGT GATGCCTGGC GGGGCGTCGC AGCCGCGGCG CACGCTGGAC GGCTGGCAGG ACTGGGCCAA GCAGCGCGGC CACCGCGGAC TGGCCTACGT GCTGGTCGCC GAAGACGGCA CGCTGGGCGG TCCGGTGGCC AAAAACCTGA CCGAGGCCGA GCGCACCGGA CTGGCCGACC ATGTCGGGGC CAAACCCGGC GATTGCATCT TCTTCTCGGC CGGTCCGGTC AAATCGTCGC GGGCACTGCT GGGCGCGGCC CGCGTCGAGA TCGCCAACCG GCTGGGCCTG ATTGACCCCG ATGCGTGGGC ATTCGTCTGG GTCGTTGACC CGCCGCTGTT CGAGCCGGCC GACGAAGCGA CCGCCGCCGG TGAGGTCGCG GTCGGCTCGG GGGCCTGGAC CGCGGTGCAC CATGCCTTCA CCGCGCCGAA ACCGGAATGG GAGGACCGCA TCGAATCCGA TACCGGCAGC GTGCTGGCCG ACGCGTACGA CATCGTCTGC AACGGCCACG AGATCGGTGG CGGCTCGGTG CGTATCCACC GCCGTGATAT CCAGGAACGG GTGTTCGCGG TGATGGGCCT GGACAAGGCC GAGGCGGAGG AAAAGTTCGG ATTCCTGTTG GAGGCGTTCA TGTTTGGCGC ACCGCCGCAC GGCGGAATCG CGTTCGGGTG GGACCGGACC ACCGCGCTGC TGGCCGGGAT GGACTCGATC CGTGAGGTGA TCGCGTTCCC GAAGACCGGC GGCGGCGTCG ATCCGCTCAC CGACGCGCCG GCGCCCATTA CCGCTCAGCA ACGTAAGGAG TCCGGAATAG ATGCCCAGCC CAAGCGGGTC CAGCAGGCAA AACAGCACGA ACAAGTTCTG CAGCCAAGCT TCTCGAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA A
Details for MytuD.00145.a.A1.PD00429
PURIFICATION DATe: 2/28/2016
CONCENTRATION: 20.24mg/ml
OBSERVED MW: 68kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM Tris pH 8.0, 200 mM NaCl, 1% Glycerol, 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 37
VIAL VOLUME: 200µl
PERCENT IDENTITY: 97
PERCENT COVERAGE: 51
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVFVLRSHA AGLLREGDAG QQVTLAGWVA RRRDHGGVIF IDLRDASGIA QVVFRDPQDT EVLAQAHRLR AEFCVSVAGV VEIRPEGNAN PEIATGEIEV NATSLTVLGE CAPLPFQLDE PAGEELRLKY RYLDLRRDDP AAAIRLRSRV NAAARAVLAR HDFVEIETPT ITRSTPEGAR DFLVPARLHP GSFYALPQSP QLFKQLLMVA GMERYYQIAR CYRDEDFRAD RQPEFTQLDM EMSFVDAXDI IAISEEVLXX LWALIGYRIP TPIPRIGYAX AMRRFGTXKP XLXFGLXL
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggtgt ttgtgctgcg cagccacgcc gcgggtttgc tacgggaagg cgacgccggg cagcaggtga cattggccgg ctgggtggct cgccgccgcg accacggcgg cgtgatcttc atcgatctgc gggacgcttc gggcatcgca caggtggtgt tccgcgaccc ccaggacact gaggtgctgg cgcaggctca ccggctgcgt gcggaattct gcgtatcggt ggccggcgtc gtcgagatcc gcccggaagg caacgccaac ccggagatcg ccaccggcga gatcgaggtc aacgccacgt cgttgaccgt gctgggcgaa tgtgcgccgc tgccgtttca gctggacgaa ccggcgggtg aggagttgcg actgaagtac cgctacctcg atctgcgccg cgacgacccc gctgcggcga ttcgactgcg ttcccgggtg aatgccgctg cgcgcgcggt gctggcgcgt cacgacttcg tcgagatcga gacgccgacg atcacccgct cgaccccgga gggagcgcgc gacttcctgg tgccggcccg actgcacccc ggttcgtttt acgccctacc gcagagcccg cagctgttca agcagctgct gatggtggcg gggatggaac gctactacca gatcgcccgc tgctaccgcg acgaggactt ccgcgccgac cgccagcccg aattcaccca gctcgatatg gagatgagct tcgtcgacgc cgangacatc atcgcgatct ccgaggaagt tctgancgan ctgtgggcgc tgataggcta ccggattccg acgcccatcc cgcggatcgg ctatgccnaa gcgatgcgcc gattcggtac gganaaaccn nacctgnggt tcgggctgga nctcgtc
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVFVLRSHA AGLLREGDAG QQVTLAGWVA RRRDHGGVIF IDLRDASGIA QVVFRDPQDT EVLAQAHRLR AEFCVSVAGV VEIRPEGNAN PEIATGEIEV NATSLTVLGE CAPLPFQLDE PAGEELRLKY RYLDLRRDDP AAAIRLRSRV NAAARAVLAR HDFVEIETPT ITRSTPEGAR DFLVPARLHP GSFYALPQSP QLFKQLLMVA GMERYYQIAR CYRDEDFRAD RQPEFTQLDM EMSFVDAEDI IAISEEVLTE LWALIGYRIP TPIPRIGYAE AMRRFGTDKP DLRFGLELVE CTDFFSDTTF RVFQAPYVGA VVMPGGASQP RRTLDGWQDW AKQRGHRGLA YVLVAEDGTL GGPVAKNLTE AERTGLADHV GAKPGDCIFF SAGPVKSSRA LLGAARVEIA NRLGLIDPDA WAFVWVVDPP LFEPADEATA AGEVAVGSGA WTAVHHAFTA PKPEWEDRIE SDTGSVLADA YDIVCNGHEI GGGSVRIHRR DIQERVFAVM GLDKAEAEEK FGFLLEAFMF GAPPHGGIAF GWDRTTALLA GMDSIREVIA FPKTGGGVDP LTDAPAPITA QQRKESGIDA QPKRVQQA
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGTTTGTG CTGCGCAGCC ACGCCGCGGG TTTGCTACGG GAAGGCGACG CCGGGCAGCA GGTGACATTG GCCGGCTGGG TGGCTCGCCG CCGCGACCAC GGCGGCGTGA TCTTCATCGA TCTGCGGGAC GCTTCGGGCA TCGCACAGGT GGTGTTCCGC GACCCCCAGG ACACTGAGGT GCTGGCGCAG GCTCACCGGC TGCGTGCGGA ATTCTGCGTA TCGGTGGCCG GCGTCGTCGA GATCCGCCCG GAAGGCAACG CCAACCCGGA GATCGCCACC GGCGAGATCG AGGTCAACGC CACGTCGTTG ACCGTGCTGG GCGAATGTGC GCCGCTGCCG TTTCAGCTGG ACGAACCGGC GGGTGAGGAG TTGCGACTGA AGTACCGCTA CCTCGATCTG CGCCGCGACG ACCCCGCTGC GGCGATTCGA CTGCGTTCCC GGGTGAATGC CGCTGCGCGC GCGGTGCTGG CGCGTCACGA CTTCGTCGAG ATCGAGACGC CGACGATCAC CCGCTCGACC CCGGAGGGAG CGCGCGACTT CCTGGTGCCG GCCCGACTGC ACCCCGGTTC GTTTTACGCC CTACCGCAGA GCCCGCAGCT GTTCAAGCAG CTGCTGATGG TGGCGGGGAT GGAACGCTAC TACCAGATCG CCCGCTGCTA CCGCGACGAG GACTTCCGCG CCGACCGCCA GCCCGAATTC ACCCAGCTCG ATATGGAGAT GAGCTTCGTC GACGCCGAGG ACATCATCGC GATCTCCGAG GAAGTTCTGA CCGAGCTGTG GGCGCTGATA GGCTACCGGA TTCCGACGCC CATCCCGCGG ATCGGCTATG CCGAAGCGAT GCGCCGATTC GGTACGGACA AACCCGACCT GCGGTTCGGG CTGGAGCTCG TCGAATGCAC GGATTTCTTC TCCGACACCA CATTTCGTGT CTTCCAGGCA CCGTATGTGG GCGCGGTGGT GATGCCTGGC GGGGCGTCGC AGCCGCGGCG CACGCTGGAC GGCTGGCAGG ACTGGGCCAA GCAGCGCGGC CACCGCGGAC TGGCCTACGT GCTGGTCGCC GAAGACGGCA CGCTGGGCGG TCCGGTGGCC AAAAACCTGA CCGAGGCCGA GCGCACCGGA CTGGCCGACC ATGTCGGGGC CAAACCCGGC GATTGCATCT TCTTCTCGGC CGGTCCGGTC AAATCGTCGC GGGCACTGCT GGGCGCGGCC CGCGTCGAGA TCGCCAACCG GCTGGGCCTG ATTGACCCCG ATGCGTGGGC ATTCGTCTGG GTCGTTGACC CGCCGCTGTT CGAGCCGGCC GACGAAGCGA CCGCCGCCGG TGAGGTCGCG GTCGGCTCGG GGGCCTGGAC CGCGGTGCAC CATGCCTTCA CCGCGCCGAA ACCGGAATGG GAGGACCGCA TCGAATCCGA TACCGGCAGC GTGCTGGCCG ACGCGTACGA CATCGTCTGC AACGGCCACG AGATCGGTGG CGGCTCGGTG CGTATCCACC GCCGTGATAT CCAGGAACGG GTGTTCGCGG TGATGGGCCT GGACAAGGCC GAGGCGGAGG AAAAGTTCGG ATTCCTGTTG GAGGCGTTCA TGTTTGGCGC ACCGCCGCAC GGCGGAATCG CGTTCGGGTG GGACCGGACC ACCGCGCTGC TGGCCGGGAT GGACTCGATC CGTGAGGTGA TCGCGTTCCC GAAGACCGGC GGCGGCGTCG ATCCGCTCAC CGACGCGCCG GCGCCCATTA CCGCTCAGCA ACGTAAGGAG TCCGGAATAG ATGCCCAGCC CAAGCGGGTC CAGCAGGCAA AACAGCACGA ACAAGTTCTG CAGCCAAGCT TCTCGAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA A
Details for MytuD.00145.a.A1.PD00487
PURIFICATION DATe: 3/7/2016
CONCENTRATION: 19.9mg/ml
OBSERVED MW: 68kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25mM tris pH 8.0, 200mM NaCl, 1% glycerol, 1mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 29
VIAL VOLUME: 200µl
PERCENT IDENTITY: 97
PERCENT COVERAGE: 51
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVFVLRSHA AGLLREGDAG QQVTLAGWVA RRRDHGGVIF IDLRDASGIA QVVFRDPQDT EVLAQAHRLR AEFCVSVAGV VEIRPEGNAN PEIATGEIEV NATSLTVLGE CAPLPFQLDE PAGEELRLKY RYLDLRRDDP AAAIRLRSRV NAAARAVLAR HDFVEIETPT ITRSTPEGAR DFLVPARLHP GSFYALPQSP QLFKQLLMVA GMERYYQIAR CYRDEDFRAD RQPEFTQLDM EMSFVDAXDI IAISEEVLXX LWALIGYRIP TPIPRIGYAX AMRRFGTXKP XLXFGLXL
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggtgt ttgtgctgcg cagccacgcc gcgggtttgc tacgggaagg cgacgccggg cagcaggtga cattggccgg ctgggtggct cgccgccgcg accacggcgg cgtgatcttc atcgatctgc gggacgcttc gggcatcgca caggtggtgt tccgcgaccc ccaggacact gaggtgctgg cgcaggctca ccggctgcgt gcggaattct gcgtatcggt ggccggcgtc gtcgagatcc gcccggaagg caacgccaac ccggagatcg ccaccggcga gatcgaggtc aacgccacgt cgttgaccgt gctgggcgaa tgtgcgccgc tgccgtttca gctggacgaa ccggcgggtg aggagttgcg actgaagtac cgctacctcg atctgcgccg cgacgacccc gctgcggcga ttcgactgcg ttcccgggtg aatgccgctg cgcgcgcggt gctggcgcgt cacgacttcg tcgagatcga gacgccgacg atcacccgct cgaccccgga gggagcgcgc gacttcctgg tgccggcccg actgcacccc ggttcgtttt acgccctacc gcagagcccg cagctgttca agcagctgct gatggtggcg gggatggaac gctactacca gatcgcccgc tgctaccgcg acgaggactt ccgcgccgac cgccagcccg aattcaccca gctcgatatg gagatgagct tcgtcgacgc cgangacatc atcgcgatct ccgaggaagt tctgancgan ctgtgggcgc tgataggcta ccggattccg acgcccatcc cgcggatcgg ctatgccnaa gcgatgcgcc gattcggtac gganaaaccn nacctgnggt tcgggctgga nctcgtc
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVFVLRSHA AGLLREGDAG QQVTLAGWVA RRRDHGGVIF IDLRDASGIA QVVFRDPQDT EVLAQAHRLR AEFCVSVAGV VEIRPEGNAN PEIATGEIEV NATSLTVLGE CAPLPFQLDE PAGEELRLKY RYLDLRRDDP AAAIRLRSRV NAAARAVLAR HDFVEIETPT ITRSTPEGAR DFLVPARLHP GSFYALPQSP QLFKQLLMVA GMERYYQIAR CYRDEDFRAD RQPEFTQLDM EMSFVDAEDI IAISEEVLTE LWALIGYRIP TPIPRIGYAE AMRRFGTDKP DLRFGLELVE CTDFFSDTTF RVFQAPYVGA VVMPGGASQP RRTLDGWQDW AKQRGHRGLA YVLVAEDGTL GGPVAKNLTE AERTGLADHV GAKPGDCIFF SAGPVKSSRA LLGAARVEIA NRLGLIDPDA WAFVWVVDPP LFEPADEATA AGEVAVGSGA WTAVHHAFTA PKPEWEDRIE SDTGSVLADA YDIVCNGHEI GGGSVRIHRR DIQERVFAVM GLDKAEAEEK FGFLLEAFMF GAPPHGGIAF GWDRTTALLA GMDSIREVIA FPKTGGGVDP LTDAPAPITA QQRKESGIDA QPKRVQQA
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGTTTGTG CTGCGCAGCC ACGCCGCGGG TTTGCTACGG GAAGGCGACG CCGGGCAGCA GGTGACATTG GCCGGCTGGG TGGCTCGCCG CCGCGACCAC GGCGGCGTGA TCTTCATCGA TCTGCGGGAC GCTTCGGGCA TCGCACAGGT GGTGTTCCGC GACCCCCAGG ACACTGAGGT GCTGGCGCAG GCTCACCGGC TGCGTGCGGA ATTCTGCGTA TCGGTGGCCG GCGTCGTCGA GATCCGCCCG GAAGGCAACG CCAACCCGGA GATCGCCACC GGCGAGATCG AGGTCAACGC CACGTCGTTG ACCGTGCTGG GCGAATGTGC GCCGCTGCCG TTTCAGCTGG ACGAACCGGC GGGTGAGGAG TTGCGACTGA AGTACCGCTA CCTCGATCTG CGCCGCGACG ACCCCGCTGC GGCGATTCGA CTGCGTTCCC GGGTGAATGC CGCTGCGCGC GCGGTGCTGG CGCGTCACGA CTTCGTCGAG ATCGAGACGC CGACGATCAC CCGCTCGACC CCGGAGGGAG CGCGCGACTT CCTGGTGCCG GCCCGACTGC ACCCCGGTTC GTTTTACGCC CTACCGCAGA GCCCGCAGCT GTTCAAGCAG CTGCTGATGG TGGCGGGGAT GGAACGCTAC TACCAGATCG CCCGCTGCTA CCGCGACGAG GACTTCCGCG CCGACCGCCA GCCCGAATTC ACCCAGCTCG ATATGGAGAT GAGCTTCGTC GACGCCGAGG ACATCATCGC GATCTCCGAG GAAGTTCTGA CCGAGCTGTG GGCGCTGATA GGCTACCGGA TTCCGACGCC CATCCCGCGG ATCGGCTATG CCGAAGCGAT GCGCCGATTC GGTACGGACA AACCCGACCT GCGGTTCGGG CTGGAGCTCG TCGAATGCAC GGATTTCTTC TCCGACACCA CATTTCGTGT CTTCCAGGCA CCGTATGTGG GCGCGGTGGT GATGCCTGGC GGGGCGTCGC AGCCGCGGCG CACGCTGGAC GGCTGGCAGG ACTGGGCCAA GCAGCGCGGC CACCGCGGAC TGGCCTACGT GCTGGTCGCC GAAGACGGCA CGCTGGGCGG TCCGGTGGCC AAAAACCTGA CCGAGGCCGA GCGCACCGGA CTGGCCGACC ATGTCGGGGC CAAACCCGGC GATTGCATCT TCTTCTCGGC CGGTCCGGTC AAATCGTCGC GGGCACTGCT GGGCGCGGCC CGCGTCGAGA TCGCCAACCG GCTGGGCCTG ATTGACCCCG ATGCGTGGGC ATTCGTCTGG GTCGTTGACC CGCCGCTGTT CGAGCCGGCC GACGAAGCGA CCGCCGCCGG TGAGGTCGCG GTCGGCTCGG GGGCCTGGAC CGCGGTGCAC CATGCCTTCA CCGCGCCGAA ACCGGAATGG GAGGACCGCA TCGAATCCGA TACCGGCAGC GTGCTGGCCG ACGCGTACGA CATCGTCTGC AACGGCCACG AGATCGGTGG CGGCTCGGTG CGTATCCACC GCCGTGATAT CCAGGAACGG GTGTTCGCGG TGATGGGCCT GGACAAGGCC GAGGCGGAGG AAAAGTTCGG ATTCCTGTTG GAGGCGTTCA TGTTTGGCGC ACCGCCGCAC GGCGGAATCG CGTTCGGGTG GGACCGGACC ACCGCGCTGC TGGCCGGGAT GGACTCGATC CGTGAGGTGA TCGCGTTCCC GAAGACCGGC GGCGGCGTCG ATCCGCTCAC CGACGCGCCG GCGCCCATTA CCGCTCAGCA ACGTAAGGAG TCCGGAATAG ATGCCCAGCC CAAGCGGGTC CAGCAGGCAA AACAGCACGA ACAAGTTCTG CAGCCAAGCT TCTCGAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA A
Details for MytuD.00145.a.A1.PW27581
PURIFICATION DATe: 9/22/2009
CONCENTRATION: 6mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 9
VIAL VOLUME: 200µl
PERCENT IDENTITY: 97
PERCENT COVERAGE: 51
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVFVLRSHA AGLLREGDAG QQVTLAGWVA RRRDHGGVIF IDLRDASGIA QVVFRDPQDT EVLAQAHRLR AEFCVSVAGV VEIRPEGNAN PEIATGEIEV NATSLTVLGE CAPLPFQLDE PAGEELRLKY RYLDLRRDDP AAAIRLRSRV NAAARAVLAR HDFVEIETPT ITRSTPEGAR DFLVPARLHP GSFYALPQSP QLFKQLLMVA GMERYYQIAR CYRDEDFRAD RQPEFTQLDM EMSFVDAXDI IAISEEVLXX LWALIGYRIP TPIPRIGYAX AMRRFGTXKP XLXFGLXL
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggtgt ttgtgctgcg cagccacgcc gcgggtttgc tacgggaagg cgacgccggg cagcaggtga cattggccgg ctgggtggct cgccgccgcg accacggcgg cgtgatcttc atcgatctgc gggacgcttc gggcatcgca caggtggtgt tccgcgaccc ccaggacact gaggtgctgg cgcaggctca ccggctgcgt gcggaattct gcgtatcggt ggccggcgtc gtcgagatcc gcccggaagg caacgccaac ccggagatcg ccaccggcga gatcgaggtc aacgccacgt cgttgaccgt gctgggcgaa tgtgcgccgc tgccgtttca gctggacgaa ccggcgggtg aggagttgcg actgaagtac cgctacctcg atctgcgccg cgacgacccc gctgcggcga ttcgactgcg ttcccgggtg aatgccgctg cgcgcgcggt gctggcgcgt cacgacttcg tcgagatcga gacgccgacg atcacccgct cgaccccgga gggagcgcgc gacttcctgg tgccggcccg actgcacccc ggttcgtttt acgccctacc gcagagcccg cagctgttca agcagctgct gatggtggcg gggatggaac gctactacca gatcgcccgc tgctaccgcg acgaggactt ccgcgccgac cgccagcccg aattcaccca gctcgatatg gagatgagct tcgtcgacgc cgangacatc atcgcgatct ccgaggaagt tctgancgan ctgtgggcgc tgataggcta ccggattccg acgcccatcc cgcggatcgg ctatgccnaa gcgatgcgcc gattcggtac gganaaaccn nacctgnggt tcgggctgga nctcgtc
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVFVLRSHA AGLLREGDAG QQVTLAGWVA RRRDHGGVIF IDLRDASGIA QVVFRDPQDT EVLAQAHRLR AEFCVSVAGV VEIRPEGNAN PEIATGEIEV NATSLTVLGE CAPLPFQLDE PAGEELRLKY RYLDLRRDDP AAAIRLRSRV NAAARAVLAR HDFVEIETPT ITRSTPEGAR DFLVPARLHP GSFYALPQSP QLFKQLLMVA GMERYYQIAR CYRDEDFRAD RQPEFTQLDM EMSFVDAEDI IAISEEVLTE LWALIGYRIP TPIPRIGYAE AMRRFGTDKP DLRFGLELVE CTDFFSDTTF RVFQAPYVGA VVMPGGASQP RRTLDGWQDW AKQRGHRGLA YVLVAEDGTL GGPVAKNLTE AERTGLADHV GAKPGDCIFF SAGPVKSSRA LLGAARVEIA NRLGLIDPDA WAFVWVVDPP LFEPADEATA AGEVAVGSGA WTAVHHAFTA PKPEWEDRIE SDTGSVLADA YDIVCNGHEI GGGSVRIHRR DIQERVFAVM GLDKAEAEEK FGFLLEAFMF GAPPHGGIAF GWDRTTALLA GMDSIREVIA FPKTGGGVDP LTDAPAPITA QQRKESGIDA QPKRVQQA
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGTTTGTG CTGCGCAGCC ACGCCGCGGG TTTGCTACGG GAAGGCGACG CCGGGCAGCA GGTGACATTG GCCGGCTGGG TGGCTCGCCG CCGCGACCAC GGCGGCGTGA TCTTCATCGA TCTGCGGGAC GCTTCGGGCA TCGCACAGGT GGTGTTCCGC GACCCCCAGG ACACTGAGGT GCTGGCGCAG GCTCACCGGC TGCGTGCGGA ATTCTGCGTA TCGGTGGCCG GCGTCGTCGA GATCCGCCCG GAAGGCAACG CCAACCCGGA GATCGCCACC GGCGAGATCG AGGTCAACGC CACGTCGTTG ACCGTGCTGG GCGAATGTGC GCCGCTGCCG TTTCAGCTGG ACGAACCGGC GGGTGAGGAG TTGCGACTGA AGTACCGCTA CCTCGATCTG CGCCGCGACG ACCCCGCTGC GGCGATTCGA CTGCGTTCCC GGGTGAATGC CGCTGCGCGC GCGGTGCTGG CGCGTCACGA CTTCGTCGAG ATCGAGACGC CGACGATCAC CCGCTCGACC CCGGAGGGAG CGCGCGACTT CCTGGTGCCG GCCCGACTGC ACCCCGGTTC GTTTTACGCC CTACCGCAGA GCCCGCAGCT GTTCAAGCAG CTGCTGATGG TGGCGGGGAT GGAACGCTAC TACCAGATCG CCCGCTGCTA CCGCGACGAG GACTTCCGCG CCGACCGCCA GCCCGAATTC ACCCAGCTCG ATATGGAGAT GAGCTTCGTC GACGCCGAGG ACATCATCGC GATCTCCGAG GAAGTTCTGA CCGAGCTGTG GGCGCTGATA GGCTACCGGA TTCCGACGCC CATCCCGCGG ATCGGCTATG CCGAAGCGAT GCGCCGATTC GGTACGGACA AACCCGACCT GCGGTTCGGG CTGGAGCTCG TCGAATGCAC GGATTTCTTC TCCGACACCA CATTTCGTGT CTTCCAGGCA CCGTATGTGG GCGCGGTGGT GATGCCTGGC GGGGCGTCGC AGCCGCGGCG CACGCTGGAC GGCTGGCAGG ACTGGGCCAA GCAGCGCGGC CACCGCGGAC TGGCCTACGT GCTGGTCGCC GAAGACGGCA CGCTGGGCGG TCCGGTGGCC AAAAACCTGA CCGAGGCCGA GCGCACCGGA CTGGCCGACC ATGTCGGGGC CAAACCCGGC GATTGCATCT TCTTCTCGGC CGGTCCGGTC AAATCGTCGC GGGCACTGCT GGGCGCGGCC CGCGTCGAGA TCGCCAACCG GCTGGGCCTG ATTGACCCCG ATGCGTGGGC ATTCGTCTGG GTCGTTGACC CGCCGCTGTT CGAGCCGGCC GACGAAGCGA CCGCCGCCGG TGAGGTCGCG GTCGGCTCGG GGGCCTGGAC CGCGGTGCAC CATGCCTTCA CCGCGCCGAA ACCGGAATGG GAGGACCGCA TCGAATCCGA TACCGGCAGC GTGCTGGCCG ACGCGTACGA CATCGTCTGC AACGGCCACG AGATCGGTGG CGGCTCGGTG CGTATCCACC GCCGTGATAT CCAGGAACGG GTGTTCGCGG TGATGGGCCT GGACAAGGCC GAGGCGGAGG AAAAGTTCGG ATTCCTGTTG GAGGCGTTCA TGTTTGGCGC ACCGCCGCAC GGCGGAATCG CGTTCGGGTG GGACCGGACC ACCGCGCTGC TGGCCGGGAT GGACTCGATC CGTGAGGTGA TCGCGTTCCC GAAGACCGGC GGCGGCGTCG ATCCGCTCAC CGACGCGCCG GCGCCCATTA CCGCTCAGCA ACGTAAGGAG TCCGGAATAG ATGCCCAGCC CAAGCGGGTC CAGCAGGCAA AACAGCACGA ACAAGTTCTG CAGCCAAGCT TCTCGAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA A