MymaA.00145.a

Aspartyl-tRNA synthetase AspS

CENTER ID: MymaA.00145.a
ORGANISM: Mycobacterium marinum ATCC BAA-535 / M
ASSOCIATED DISEASE: Cutaneous ulcerations, deep abscesses
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MymaA.00145.a.A1.GE29952 Full length( MymaA.00145.a ) 1 593
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
OrthoMCL: OG5_127662
PATRIC ID: fig|216594.6.peg.2308
RefSeq: YP_001850462.1
UniProt: B2HNA9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MFVLRSHAAG ALRDSDAGRQ VTLAGWVARR RDHGGVIFID LRDASGIAQV VFREADVLAQ AHRLRAEFCV AVTGVVEIRP EGNANPEIAT GDIEVNASSL TVLGECAPLP FQLDEPAGEE LRLKYRYLDL RRDGPASAIR LRSKVNAAAR AVLAGHDFVE IETPTITRST PEGARDFLVP ARLHPGMFYA LPQSPQLFKQ LLMVAGMERY YQIARCYRDE DFRADRQPEF TQLDMELSFV DVEDIIAVSE EILTALWALI GYRIPTPIPR ISYADAMRRF GSDKPDLRFG LELVECAEFF KDTTFRVFQA PYVGAVVMPG GASQPRRTLD GWQEWAKQRG HRGLAYVLVG EDGTLGGPVA KNLTDAERDG LAAHVGASPG DCIFFSAGPV KASRALLGAA RIEIAERLNL IDPSAWEFVW VVDPPLFEPA EDATASGDVA VGSGAWTAVH HAFTAPKPEL EDRIESDPGS VLADAYDIVC NGHEIGGGSI RIHRRDIQER VFAVMGLSKA EAEEKFGFLL EAFTFGAPPH GGIAFGWDRV STLLAGADSI RDVIAFPKTG GGVDPLTEAP APITAQQRKE AGIDAKPKQV EGT
NT Sequence
GTGTTTGTGC TGCGTAGCCA CGCCGCTGGG GCATTACGGG ATAGCGATGC CGGGCGACAG GTGACGCTGG CCGGTTGGGT GGCCCGCCGC CGCGACCATG GCGGCGTCAT CTTCATCGAC CTGCGCGACG CCTCCGGCAT TGCCCAGGTT GTATTCCGCG AAGCCGACGT GTTGGCCCAG GCGCATCGGC TGCGTGCCGA GTTCTGCGTC GCGGTCACCG GTGTCGTCGA GATCCGTCCC GAAGGCAACG CCAACCCCGA GATCGCGACC GGCGACATCG AGGTCAACGC CTCTTCATTG ACGGTGCTCG GCGAATGCGC GCCACTGCCG TTCCAGCTCG ACGAGCCCGC GGGGGAGGAG TTGCGGCTGA AATACCGCTA CCTCGACCTG CGCCGCGACG GTCCGGCATC GGCGATTCGG TTGCGTTCCA AGGTCAATGC CGCCGCCCGC GCGGTGCTGG CCGGACACGA CTTCGTCGAG ATCGAAACAC CGACGATCAC CCGCTCCACC CCGGAGGGCG CCCGCGACTT TCTGGTGCCC GCCCGGCTGC ACCCGGGCAT GTTCTACGCG CTGCCGCAAA GCCCGCAGCT GTTCAAACAG CTGCTCATGG TGGCCGGGAT GGAGCGCTAC TACCAGATCG CCCGGTGCTA CCGCGACGAG GACTTCCGGG CCGATCGCCA GCCCGAGTTC ACCCAGCTCG ACATGGAATT GAGCTTTGTC GACGTCGAGG ACATCATCGC GGTCTCCGAG GAGATCCTGA CCGCGCTGTG GGCGCTGATC GGCTACCGGA TTCCGACCCC CATCCCACGG ATCAGCTACG CCGACGCGAT GCGCCGGTTC GGGTCCGACA AACCCGACCT GCGGTTCGGA CTCGAACTCG TGGAGTGCGC AGAGTTCTTC AAAGACACCA CATTCCGGGT GTTCCAAGCG CCCTACGTCG GCGCGGTGGT GATGCCCGGC GGAGCCTCGC AGCCGCGGCG CACGCTGGAT GGCTGGCAAG AGTGGGCCAA ACAGCGCGGT CACCGCGGGC TGGCCTACGT GCTGGTCGGC GAGGACGGGA CGCTGGGCGG CCCGGTGGCC AAGAACCTGA CCGATGCCGA GCGTGACGGA CTGGCCGCTC ACGTGGGCGC CAGTCCGGGG GACTGCATCT TCTTCTCGGC GGGACCGGTG AAGGCGTCGC GGGCGCTGCT GGGGGCGGCG CGAATCGAGA TCGCCGAGCG GCTCAACCTG ATCGACCCCA GCGCCTGGGA GTTCGTTTGG GTGGTTGATC CGCCCCTGTT CGAGCCCGCC GAGGACGCCA CCGCCTCCGG CGACGTGGCC GTTGGATCCG GCGCGTGGAC GGCGGTGCAT CACGCGTTTA CCGCGCCCAA GCCAGAGCTC GAAGACCGTA TCGAATCGGA CCCCGGCAGC GTGTTGGCCG ATGCCTACGA CATCGTCTGC AATGGCCACG AGATCGGGGG TGGATCGATC CGTATCCATC GTCGCGACAT TCAGGAGCGG GTGTTCGCGG TCATGGGTCT GAGCAAGGCT GAGGCCGAAG AGAAGTTCGG CTTCCTGCTG GAGGCTTTCA CCTTCGGCGC CCCGCCGCAT GGCGGCATCG CGTTCGGGTG GGACCGGGTC AGCACGTTGC TGGCCGGAGC CGATTCGATC CGCGATGTGA TCGCGTTCCC CAAAACCGGT GGCGGTGTCG ACCCGCTTAC CGAGGCCCCC GCGCCGATCA CCGCCCAGCA GCGCAAGGAG GCTGGAATAG ACGCCAAGCC CAAACAAGTT GAGGGGACA
Details for MymaA.00145.a.A1.GE29952
HARVESTED ON: 7/13/2010
SEQUENCED ON: 8/18/2010
EXPECTED MW: 66kDa
OBSERVED MW: 70kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Few (1-10)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 44
Validated AA Sequence
MPGGASQPRR TLDGWQEWAK QRGHRGLAYV LVGEDGTLGG PVAKNLTDAE RDGLAAHVGA SPGDCIFFSA GPVKASRALL GAARIEIAER LNLIDPSAWE FVWVVDPPLF EPAEDATASG DVAVGSGAWT AVHHAFTAPK PELEDRIESD PGSVLADAYD IVCNGHEIGG GSIRIHRRDI QERVFAVMGL SKAEAEEKFG FLLEAFTFGA PPHGGIAFGW DRVSTLLAGA DSIRDVIAFP KTGGGVDPLT EAPAPITAQQ RKEAGIDAKP KQV
Validated NT Sequence
caacttgttt gggcttggcg tctattccag cctccttgcg ctgctgggcg gtgatcggcg cgggggcctc ggtaagcggg tcgacaccgc caccggtttt ggggaacgcg atcacatcgc ggatcgaatc ggctccggcc agcaacgtgc tgacccggtc ccacccgaac gcgatgccgc catgcggcgg ggcgccgaag gtgaaagcct ccagcaggaa gccgaacttc tcttcggcct cagccttgct cagacccatg accgcgaaca cccgctcctg aatgtcgcga cgatggatac ggatcgatcc acccccgatc tcgtggccat tgcagacgat gtcgtaggca tcggccaaca cgctgccggg gtccgattcg atacggtctt cgagctctgg cttgggcgcg gtaaacgcgt gatgcaccgc cgtccacgcg ccggatccaa cggccacgtc gccggaggcg gtggcgtcct cggcgggctc gaacaggggc ggatcaacca cccaaacgaa ctcccaggcg ctggggtcga tcaggttgag ccgctcggcg atctcgattc gcgccgcccc cagcagcgcc cgcgacgcct tcaccggtcc cgccgagaag aagatgcagt cccccggact ggcgcccacg tgagcggcca gtccgtcacg ctcggcatcg gtcaggttct tggccaccgg gccgcccagc gtcccgtcct cgccgaccag cacgtangcc agcccgcggt gaccgcgctg tttggcccac tcttgccagc catccagcgt gcgccgcggc tgcgaggctc cgccnggcat caccaccgcg ccnacgt
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVFVLRSHA AGALRDSDAG RQVTLAGWVA RRRDHGGVIF IDLRDASGIA QVVFREADVL AQAHRLRAEF CVAVTGVVEI RPEGNANPEI ATGDIEVNAS SLTVLGECAP LPFQLDEPAG EELRLKYRYL DLRRDGPASA IRLRSKVNAA ARAVLAGHDF VEIETPTITR STPEGARDFL VPARLHPGMF YALPQSPQLF KQLLMVAGME RYYQIARCYR DEDFRADRQP EFTQLDMELS FVDVEDIIAV SEEILTALWA LIGYRIPTPI PRISYADAMR RFGSDKPDLR FGLELVECAE FFKDTTFRVF QAPYVGAVVM PGGASQPRRT LDGWQEWAKQ RGHRGLAYVL VGEDGTLGGP VAKNLTDAER DGLAAHVGAS PGDCIFFSAG PVKASRALLG AARIEIAERL NLIDPSAWEF VWVVDPPLFE PAEDATASGD VAVGSGAWTA VHHAFTAPKP ELEDRIESDP GSVLADAYDI VCNGHEIGGG SIRIHRRDIQ ERVFAVMGLS KAEAEEKFGF LLEAFTFGAP PHGGIAFGWD RVSTLLAGAD SIRDVIAFPK TGGGVDPLTE APAPITAQQR KEAGIDAKPK QVEG
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgtttgtg ctgcgtagcc acgccgctgg ggcattacgg gatagcgatg ccgggcgaca ggtgacgctg gccggttggg tggcccgccg ccgcgaccat ggcggcgtca tcttcatcga cctgcgcgac gcctccggca ttgcccaggt tgtattccgc gaagccgacg tgttggccca ggcgcatcgg ctgcgtgccg agttctgcgt cgcggtcacc ggtgtcgtcg agatccgtcc cgaaggcaac gccaaccccg agatcgcgac cggcgacatc gaggtcaacg cctcttcatt gacggtgctc ggcgaatgcg cgccactgcc gttccagctc gacgagcccg cgggggagga gttgcggctg aaataccgct acctcgacct gcgccgcgac ggtccggcat cggcgattcg gttgcgttcc aaggtcaatg ccgccgcccg cgcggtgctg gccggacacg acttcgtcga gatcgaaaca ccgacgatca cccgctccac cccggagggc gcccgcgact ttctggtgcc cgcccggctg cacccgggca tgttctacgc gctgccgcaa agcccgcagc tgttcaaaca gctgctcatg gtggccggga tggagcgcta ctaccagatc gcccggtgct accgcgacga ggacttccgg gccgatcgcc agcccgagtt cacccagctc gacatggaat tgagctttgt cgacgtcgag gacatcatcg cggtctccga ggagatcctg accgcgctgt gggcgctgat cggctaccgg attccgaccc ccatcccacg gatcagctac gccgacgcga tgcgccggtt cgggtccgac aaacccgacc tgcggttcgg actcgaactc gtggagtgcg cagagttctt caaagacacc acattccggg tgttccaagc gccctacgtc ggcgcggtgg tgatgcccgg cggagcctcg cagccgcggc gcacgctgga tggctggcaa gagtgggcca aacagcgcgg tcaccgcggg ctggcctacg tgctggtcgg cgaggacggg acgctgggcg gcccggtggc caagaacctg accgatgccg agcgtgacgg actggccgct cacgtgggcg ccagtccggg ggactgcatc ttcttctcgg cgggaccggt gaaggcgtcg cgggcgctgc tgggggcggc gcgaatcgag atcgccgagc ggctcaacct gatcgacccc agcgcctggg agttcgtttg ggtggttgat ccgcccctgt tcgagcccgc cgaggacgcc accgcctccg gcgacgtggc cgttggatcc ggcgcgtgga cggcggtgca tcacgcgttt accgcgccca agccagagct cgaagaccgt atcgaatcgg accccggcag cgtgttggcc gatgcctacg acatcgtctg caatggccac gagatcgggg gtggatcgat ccgtatccat cgtcgcgaca ttcaggagcg ggtgttcgcg gtcatgggtc tgagcaaggc tgaggccgaa gagaagttcg gcttcctgct ggaggctttc accttcggcg ccccgccgca tggcggcatc gcgttcgggt gggaccgggt cagcacgttg ctggccggag ccgattcgat ccgcgatgtg atcgcgttcc ccaaaaccgg tggcggtgtc gacccgctta ccgaggcccc cgcgccgatc accgcccagc agcgcaagga ggctggaata gacgccaagc ccaaacaagt tgaggggaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa