MyleA.00145.a

Aspartyl-tRNA synthetase

CENTER ID: MyleA.00145.a
ORGANISM: Mycobacterium leprae Br4923
ASSOCIATED DISEASE: Hansen's disease, leprosy
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyleA.00145.a.B1.GE37569 Full length( MyleA.00145.a ) 1 589
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
OrthoMCL: OG5_127662
PATRIC ID: fig|561304.4.peg.874
RefSeq: YP_002503065.1
UniProt: B8ZUJ3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLRSHGAGVL RKSDAGQQVT LAGWVSRRRD HGGVIFIDLR DSSGITQAVF REPDVLAQAH RLRAEFCIAV SGVVEIRPEG NANAEIPTGD IEVNATSLTV LGESAPLPFQ LDEPAGEELR LKYRYLDLRR DGPGSAIRLR SKVNATARAV LARHDFVEIE TPTITRSTPE GARDFLVPAR LRPGTFYALP QSPQLFKQLL MVAGMERYYQ IAHCYRDEDF RADRQPEFTQ LDMEMSFVDA EDVIAISEEI LTELWMLIGY HIPAPIPRIS YADAMRRFGS DKPDLRFGLE LVECTEFFCD TTFRVFQAPY VGAVVMPGGA AQPRRTLDEW QDWAKQRGHR GLAYVLVTDD GTLGGPVAKN FSDAERSRLA SHVGAEPGDC IFFSAGPAKS SRALLGAARG EIANRLGLID PEAWAFVWVV DPPLFERADE ATKVGEMAVG SGAWTAVHHA FTSPKPEFED SIESDPGSVL ADAYDIVCNG HEIGSGSVRI NRRDIQERVF AVMGLEKAEA EEKFGFLLEA FTFGAPPHGG IAFGWDRTNA LLAGMESIRE VIAFPKTGGG VDPLTDAPAS ITAQQRKESG IDTKPKEVE
NT Sequence
GTGCTGCGTA GCCACGGCGC TGGTGTGTTA CGGAAAAGCG ATGCCGGGCA GCAGGTAACA TTAGCTGGCT GGGTGTCTCG CCGTCGAGAC CACGGCGGCG TCATCTTCAT TGATCTGCGC GACTCCTCGG GCATCACACA GGCGGTGTTT CGCGAGCCTG ACGTGTTGGC CCAGGCGCAT CGGCTGCGCG CTGAGTTCTG CATCGCGGTT TCCGGCGTAG TCGAGATTCG CCCCGAGGGT AACGCCAATG CCGAGATTCC CACCGGCGAT ATCGAGGTCA ACGCCACTTC GCTGACCGTG CTCGGTGAGA GTGCGCCGCT ACCATTCCAA CTCGATGAGC CTGCGGGTGA GGAATTGCGG TTGAAGTACC GTTACCTCGA CCTGCGCCGC GACGGTCCTG GATCGGCAAT TCGGTTGCGT TCCAAGGTGA ATGCCACTGC TCGTGCAGTG CTGGCGCGCC ACGATTTCGT TGAGATCGAA ACCCCGACGA TCACCCGCTC AACCCCGGAG GGGGCGCGCG ACTTTTTAGT TCCGGCCCGG TTGCGGCCGG GCACGTTCTA TGCTCTGCCG CAGAGCCCGC AATTGTTCAA ACAGTTGCTG ATGGTGGCCG GGATGGAGCG TTATTACCAG ATTGCCCACT GCTACCGCGA CGAGGATTTC CGTGCCGACC GCCAGCCCGA ATTCACTCAG CTCGACATGG AGATGAGCTT CGTCGATGCC GAGGATGTCA TCGCGATCTC CGAGGAAATC CTAACCGAGC TCTGGATGCT GATAGGCTAC CACATTCCGG CGCCGATCCC GCGGATCAGC TATGCGGATG CTATGCGACG GTTCGGCTCT GACAAGCCCG ACTTGCGGTT CGGGCTGGAG CTTGTTGAAT GCACAGAGTT CTTCTGCGAC ACAACGTTCC GTGTCTTCCA GGCACCGTAT GTCGGTGCGG TAGTGATGCC CGGCGGTGCT GCACAGCCGC GGCGCACACT GGACGAGTGG CAGGACTGGG CCAAACAGCG CGGGCATCGC GGCCTGGCCT ACGTGCTGGT GACCGATGAC GGTACGCTCG GCGGACCGGT GGCCAAGAAC TTCAGCGATG CCGAACGCTC TCGCCTGGCC TCCCACGTAG GCGCCGAACC CGGGGACTGC ATCTTCTTTT CAGCGGGCCC GGCGAAGTCG TCGCGGGCAC TGCTGGGGGC CGCCCGCGGT GAGATCGCCA ACCGGCTGGG TTTGATCGAC CCCGAGGCCT GGGCGTTCGT CTGGGTAGTC GACCCGCCGC TTTTCGAACG CGCCGACGAG GCCACCAAGG TCGGCGAAAT GGCAGTGGGC TCTGGTGCGT GGACTGCGGT GCATCATGCC TTCACATCGC CGAAACCGGA ATTCGAGGAC AGCATAGAGT CCGATCCCGG CAGCGTGTTG GCCGATGCCT ACGACATCGT TTGTAACGGC CACGAGATTG GTAGCGGCTC GGTTCGTATT AACCGCCGTG ATATCCAGGA ACGGGTGTTT GCGGTTATGG GATTGGAAAA GGCTGAGGCG GAGGAGAAAT TCGGGTTCCT TTTGGAGGCT TTTACGTTTG GTGCGCCTCC GCACGGCGGT ATCGCCTTTG GCTGGGATCG GACCAATGCC TTGCTGGCCG GGATGGAGTC GATACGTGAG GTGATTGCGT TCCCCAAGAC CGGCGGTGGT GTCGACCCGC TCACTGATGC GCCGGCTTCG ATAACCGCTC AGCAGCGCAA GGAATCCGGA ATAGACACCA AGCCCAAAGA GGTTGAG
Details for MyleA.00145.a.B1.GE37569
HARVESTED ON: 11/25/2013
SEQUENCED ON: 12/4/2013
EXPECTED MW: 66kDa
OBSERVED MW: 70kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 91
PERCENT COVERAGE: 51
Validated AA Sequence
MRMLCDGSAL TSPTCGSGWS LLNAQSSSAT QRFRVFQAPY VGAVVMPGGA AQPRRTLDEW QDWXKQRGHR GLAYVLVTDD GTLGGPVAKN FSDAERSRLA SHVGAEPGDC IFFSAGPAKS SRALLGAARG EIANRLGLID PEAWAFVWVV DPPLFERADE ATKVGEMAVG SGAWTAVHHA FTSPKPEFED SIESDPGSVL ADAYDIVCNG HEIGSGSVRI NRRDIQERVF AVMGLEKAEA EEKFGFLLEA FTFGAPPHGG IAFGWDRTNA LLAGMESIRE VIAFPKTGGG VDPLTDAPAS ITAQQRKESG
Validated NT Sequence
ttccggattc cttgcgctgc tgagcggtta tcgaagccgg cgcatcagtg agcgggtcga caccaccgcc ggtcttgggg aacgcaatca cctcacgtat cgactccatc ccggccagca aggcattggt ccgatcccag ccaaaggcga taccgccgtg cggaggcgca ccaaacgtaa aagcctccaa aaggaacccg aatttctcct ccgcctcagc cttttccaat cccataaccg caaacacccg ttcctggata tcacggcggt taatacgaac cgagccgcta ccaatctcgt ggccgttaca aacgatgtcg taggcatcgg ccaacacgct gccgggatcg gactctatgc tgtcctcgaa ttccggtttc ggcgatgtga aggcatgatg caccgcagtc cacgcaccag agcccactgc catttcgccg accttggtgg cctcgtcggc gcgttcgaaa agcggcgggt cgactaccca gacgaacgcc caggcctcgg ggtcgatcaa acccagccgg ttggcgatct caccgcgggc ggcccccagc agtgcccgcg acgacttcgc cgggcccgct gaaaagaaga tgcagtcccc gggttcggcg cctacgtggg angccaggcg agagcgttcg gcatcgctga agttcttggc caccggtccg ccgagcgtac cgtcatcggt caccagcacg tangccaggc cgcgatgccc gcgctgtttg gnccagtcct gccactcgtc cagtgtgcgc cgcggctgtg cagcaccgcc nggcatcact accgcaccga catacggtgc ctggaagaca cggaancgtt gtgtcgcaga agaactctgt gcattcaaca agctccagcc cgaaccgcaa gtcgggcttg tcagagccga accgtcgcat agcatccgca tagctgatcc gcgggatngg cgccggaatg tggtagccta tcagcatcca gagctcggtt aggatttcct cggagatcgc gatgacatcc tcggcatcga cgaagctcat ctccatgtcg agctgagtga attcgggctg gcggtcggca cggaaatcct cgtcgcggta gcagtgggca atctggtaat aacgctccat cccggccacc atcagcaact gtttgaacaa ttgcgggctc tgcggcagag catagaacgt gcccggccgc aaccgggccg gaactaaaaa gtcgcgcgcc ccctccgggg ttgagcgggt gatcgtcggg gtttcgatct caacgaaatc gtggcgcgcc agcactgcac gagcagtggc attcaccttg gaacgcaacc gaattgccga tccaggaccg tcgcggcgca ggtcgaggta acggtacttc aaccgcaatt cctcacccgc aggctcatcg agttggaatg gtagcggcgc actctcaccg agcacggtca gcgaagtggc gttgacctcg atatcgccgg tgggaatctc ggcattggcg ttaccctcgg ggcgaatctc gactacgccg gaaaccgcga tgcagaactc agcgcgcagc cgatgcgcct gggccaacac gtcaggctcg cgaaacaccg cctgtgtgat gcccgaggag tcgcgcagat caatgaagat gacgccgccg tggtctcgac ggcgagacac ccagccagct aatgttacct gctgcccggc atcgcttttc cgtaacacac cagcgccgtg gctacgcag
Expected Protein Sequence
MAHHHHHHVL RSHGAGVLRK SDAGQQVTLA GWVSRRRDHG GVIFIDLRDS SGITQAVFRE PDVLAQAHRL RAEFCIAVSG VVEIRPEGNA NAEIPTGDIE VNATSLTVLG ESAPLPFQLD EPAGEELRLK YRYLDLRRDG PGSAIRLRSK VNATARAVLA RHDFVEIETP TITRSTPEGA RDFLVPARLR PGTFYALPQS PQLFKQLLMV AGMERYYQIA HCYRDEDFRA DRQPEFTQLD MEMSFVDAED VIAISEEILT ELWMLIGYHI PAPIPRISYA DAMRRFGSDK PDLRFGLELV ECTEFFCDTT FRVFQAPYVG AVVMPGGAAQ PRRTLDEWQD WAKQRGHRGL AYVLVTDDGT LGGPVAKNFS DAERSRLASH VGAEPGDCIF FSAGPAKSSR ALLGAARGEI ANRLGLIDPE AWAFVWVVDP PLFERADEAT KVGEMAVGSG AWTAVHHAFT SPKPEFEDSI ESDPGSVLAD AYDIVCNGHE IGSGSVRINR RDIQERVFAV MGLEKAEAEE KFGFLLEAFT FGAPPHGGIA FGWDRTNALL AGMESIREVI AFPKTGGGVD PLTDAPASIT AQQRKESGID TKPKEVE
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtgctgc gtagccacgg cgctggtgtg ttacggaaaa gcgatgccgg gcagcaggta acattagctg gctgggtgtc tcgccgtcga gaccacggcg gcgtcatctt cattgatctg cgcgactcct cgggcatcac acaggcggtg tttcgcgagc ctgacgtgtt ggcccaggcg catcggctgc gcgctgagtt ctgcatcgcg gtttccggcg tagtcgagat tcgccccgag ggtaacgcca atgccgagat tcccaccggc gatatcgagg tcaacgccac ttcgctgacc gtgctcggtg agagtgcgcc gctaccattc caactcgatg agcctgcggg tgaggaattg cggttgaagt accgttacct cgacctgcgc cgcgacggtc ctggatcggc aattcggttg cgttccaagg tgaatgccac tgctcgtgca gtgctggcgc gccacgattt cgttgagatc gaaaccccga cgatcacccg ctcaaccccg gagggggcgc gcgacttttt agttccggcc cggttgcggc cgggcacgtt ctatgctctg ccgcagagcc cgcaattgtt caaacagttg ctgatggtgg ccgggatgga gcgttattac cagattgccc actgctaccg cgacgaggat ttccgtgccg accgccagcc cgaattcact cagctcgaca tggagatgag cttcgtcgat gccgaggatg tcatcgcgat ctccgaggaa atcctaaccg agctctggat gctgataggc taccacattc cggcgccgat cccgcggatc agctatgcgg atgctatgcg acggttcggc tctgacaagc ccgacttgcg gttcgggctg gagcttgttg aatgcacaga gttcttctgc gacacaacgt tccgtgtctt ccaggcaccg tatgtcggtg cggtagtgat gcccggcggt gctgcacagc cgcggcgcac actggacgag tggcaggact gggccaaaca gcgcgggcat cgcggcctgg cctacgtgct ggtgaccgat gacggtacgc tcggcggacc ggtggccaag aacttcagcg atgccgaacg ctctcgcctg gcctcccacg taggcgccga acccggggac tgcatcttct tttcagcggg cccggcgaag tcgtcgcggg cactgctggg ggccgcccgc ggtgagatcg ccaaccggct gggtttgatc gaccccgagg cctgggcgtt cgtctgggta gtcgacccgc cgcttttcga acgcgccgac gaggccacca aggtcggcga aatggcagtg ggctctggtg cgtggactgc ggtgcatcat gccttcacat cgccgaaacc ggaattcgag gacagcatag agtccgatcc cggcagcgtg ttggccgatg cctacgacat cgtttgtaac ggccacgaga ttggtagcgg ctcggttcgt attaaccgcc gtgatatcca ggaacgggtg tttgcggtta tgggattgga aaaggctgag gcggaggaga aattcgggtt ccttttggag gcttttacgt ttggtgcgcc tccgcacggc ggtatcgcct ttggctggga tcggaccaat gccttgctgg ccgggatgga gtcgatacgt gaggtgattg cgttccccaa gaccggcggt ggtgtcgacc cgctcactga tgcgccggct tcgataaccg ctcagcagcg caaggaatcc ggaatagaca ccaagcccaa agaggttgag tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat