MysmA.00145.a
Aspartyl-tRNA synthetase
| CENTER ID: | MysmA.00145.a |
| ORGANISM: | Mycobacterium smegmatis ATCC 700084 / mc(2)155 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIC |
Ordering Clones & Proteins
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MysmA.00145.a.A1.GE28570 | Full length( MysmA.00145.a ) | 1 | 598 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Structures
4O2D
DEPOSITED: 12/17/2013
DETERMINATION: XRay
CLONE: MysmA.00145.a.A1.GE28570
PROTEIN: MysmA.00145.a.A1.PS00571
Publications by SSGCID
Increasing the structural coverage of tuberculosis drug targets.
Abendroth J, Abramov A, Armour B, Barrett LK, Baugh L, Begley DW, Buchko GW, Choi R, Clifton MC, Dieterich SH, Dranow DM, Edwards TE, Fairman JW, Ferrell M, Fox D, Gardberg AS, Hewitt SN, Lorimer D, Lyons-Abbott S, Mundt E, Muruthi MM, Myers J, Myler PJ, Napuli AJ, Phan I, Sekar A, Serbzhinskiy D, Stacy R, Staker BL, Stewart LJ, Taylor BM, Thompkins K, Tran N, Van Voorhis WC, Zhang Y
Tuberculosis (Edinb) - 2014
volume 95, issue 2, pages 142-8
PMID: 25613812; PMCID: PMC4361283
PDB:
3GVC,
3GVG,
3GWC,
3H7F,
3H81,
3HE2,
3HWI,
3HWK,
3HZG,
3ICO,
3KHP,
3LLS,
3MOY,
3MPZ,
3MYB,
3NDN,
3NDO,
3NF4,
3NG3,
3NJD,
3NWO,
3O0M,
3O38,
3OC6,
3OC7,
3OEC,
3OI9,
3OKS,
3OL3,
3OL4,
3OME,
3P0T,
3P2Y,
3P4I,
3P4T,
3P5M,
3P85,
3PE8,
3PGX,
3PK0,
3PPI,
3PXX,
3PZY,
3Q1T,
3Q8N,
3QBP,
3QDF,
3QH4,
3QHA,
3QIV,
3QK8,
3QKA,
3QLJ,
3QMJ,
3QRE,
3QUA,
3QUV,
3QXI,
3QXZ,
3QYR,
3R0O,
3R1I,
3R1J,
3R20,
3R2N,
3R4T,
3R6H,
3R6O,
3R7K,
3R8C,
3R9P,
3R9Q,
3R9R,
3R9S,
3R9T,
3RD5,
3RD7,
3RD8,
3RFQ,
3RIH,
3RR2,
3RR6,
3RRP,
3RRV,
3RSI,
3RV2,
3S55,
3S82,
3SBX,
3SF6,
3SLL,
3SVK,
3SVT,
3SWO,
3SWT,
3SWX,
3SX2,
3T3W,
3T7C,
3TAV,
3TCR,
3TDE,
3TJR,
3TL3,
3TLF,
3TRR,
3TSC,
3TX2,
3TZQ,
3TZU,
3U0A,
3U0B,
3UCX,
3UVE,
4DI1,
4DIE,
4DQ8,
4DXL,
4ED4,
4EGE,
4EGF,
4EMD,
4EO9,
4EYE,
4F3W,
4F47,
4FFC,
4GK6,
4HDT,
4HR3,
4I1Y,
4IJN,
4IV6,
4IZ9,
4J5I,
4KAM,
4LGV,
4O2D,
5EJ2
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_127662 |
| PATRIC ID: | fig|246196.19.peg.2965 |
| RefSeq: | YP_887321.1 |
| UniProt: | A0QWN3 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLRTHAAGSL RPADAGQTVT LAGWVARRRD HGGVIFIDLR DASGVSQVVF REGDVLAAAH RLRAEFCVAV TGVVEVRPEG NENPEIPTGQ IEVNATELTV LGESAPLPFQ LDEQAGEEAR LKYRYLDLRR EGPGNALRLR SKVNAAARSV LAEHDFVEIE TPTLTRSTPE GARDFLVPAR LQPGSFYALP QSPQLFKQLL MVAGMERYYQ IARCYRDEDF RADRQPEFTQ LDMEMSFVEA DDVIAISEQV LKAVWATIGY DLPLPLPRIS YEEAMRRFGS DKPDLRFGIE LVECTEYFKD TTFRVFQAPY VGAVVMPGGA SQPRRTLDGW QEFAKQRGHK GLAYVLVGED GTLGGPVAKN LSDAERDGLV AHVGANPGDC IFFAAGPAKG ARALLGATRI EIAKRLDLID PNAWAFTWVV DFPMFEAADE ATAAGDVAVG SGAWTAMHHA FTAPKPDSVD TFDSDPGNAL SDAYDIVCNG NEIGGGSIRI HRRDIQERVF AMMGIDHDEA QEKFGFLLDA FSYGAPPHGG IAFGWDRITA LLAGVDSIRE VIAFPKSGGG VDPLTDAPAP ITPQQRKESG IDAKPREDKP KEDAKSKA
NT Sequence
GTGCTGCGCA CTCATGCCGC CGGTTCGTTG CGGCCCGCCG ACGCCGGTCA GACGGTGACG TTGGCGGGAT GGGTGGCGCG TCGCCGTGAT CACGGCGGCG TCATCTTCAT CGACCTGCGC GACGCGTCCG GGGTGTCGCA GGTGGTGTTC CGCGAGGGCG ACGTCCTCGC GGCCGCGCAC CGGCTGCGCG CCGAGTTCTG CGTCGCGGTG ACCGGCGTCG TCGAGGTCCG TCCCGAGGGC AACGAGAACC CCGAGATCCC CACCGGGCAG ATCGAGGTCA ACGCCACCGA GCTGACCGTG CTGGGCGAGA GCGCGCCGTT GCCGTTCCAG CTCGACGAGC AGGCCGGCGA GGAAGCGCGC CTGAAGTACC GCTACCTGGA CCTGCGCCGC GAAGGCCCCG GCAACGCACT GCGGCTGCGG TCGAAGGTCA ACGCCGCCGC ACGGTCCGTG CTCGCCGAAC ACGACTTCGT CGAGATCGAG ACCCCGACGC TGACGCGATC GACGCCCGAG GGCGCCCGCG ACTTCCTGGT GCCCGCGCGC CTGCAGCCCG GCTCGTTCTA CGCGCTGCCG CAGAGCCCGC AGCTGTTCAA GCAGCTGCTG ATGGTCGCGG GCATGGAGCG CTACTACCAG ATCGCACGCT GCTACCGCGA CGAGGACTTC CGCGCCGATC GTCAGCCCGA GTTCACCCAG CTCGACATGG AGATGAGCTT CGTCGAGGCC GACGACGTCA TCGCGATCTC CGAGCAGGTC CTCAAGGCCG TGTGGGCCAC GATCGGCTAC GACCTGCCGC TGCCGCTTCC GCGGATCAGC TACGAAGAGG CCATGCGCCG CTTCGGCTCC GACAAGCCCG ATCTGCGCTT CGGCATCGAA CTCGTCGAGT GCACCGAGTA CTTCAAAGAC ACCACGTTCC GCGTTTTCCA GGCGCCGTAC GTCGGCGCCG TCGTGATGCC CGGCGGCGCG TCGCAGCCCC GCCGCACGCT CGACGGCTGG CAGGAGTTCG CCAAGCAGCG CGGGCACAAG GGCCTGGCGT ACGTGCTCGT CGGCGAGGAC GGAACCCTCG GCGGGCCGGT GGCCAAGAAC CTGTCCGACG CCGAACGCGA CGGGCTGGTC GCGCACGTCG GCGCCAACCC GGGTGACTGC ATCTTCTTCG CGGCAGGTCC CGCCAAGGGT GCCCGCGCGC TGCTCGGCGC CACCCGCATC GAGATCGCCA AGCGCCTCGA CCTCATCGAC CCGAACGCCT GGGCGTTCAC CTGGGTGGTC GACTTCCCGA TGTTCGAGGC CGCCGACGAG GCCACGGCCG CCGGTGACGT CGCCGTCGGC TCGGGCGCCT GGACCGCGAT GCACCACGCG TTCACCGCGC CCAAGCCCGA CTCCGTCGAC ACGTTCGACT CCGATCCCGG CAACGCGCTG TCCGACGCCT ACGACATCGT GTGCAACGGC AACGAGATCG GTGGCGGCTC GATCCGTATC CACCGCCGTG ACATCCAGGA GCGGGTGTTC GCGATGATGG GCATCGATCA CGACGAGGCC CAGGAGAAGT TCGGGTTCCT GCTCGACGCG TTCTCCTACG GCGCCCCGCC GCACGGCGGC ATCGCGTTCG GCTGGGATCG CATCACCGCA CTGCTCGCCG GGGTCGATTC GATCCGCGAG GTCATCGCGT TCCCCAAGTC CGGTGGCGGG GTCGACCCGC TGACCGACGC GCCCGCACCG ATCACGCCGC AGCAGCGCAA GGAATCCGGG ATCGACGCCA AACCCAGGGA AGACAAGCCG AAGGAAGACG CCAAGTCAAA GGCG
Details for MysmA.00145.a.A1.GE28570
| HARVESTED ON: | 2/17/2010 |
| SEQUENCED ON: | 12/12/2013 |
| EXPECTED MW: | 67kDa |
| OBSERVED MW: | 70kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Few (1-10) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 98 |
| PERCENT COVERAGE: | 64 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVLRTHAAG SLRPADAGQT VTLAGWVARR RDHGGVIFID LRDASGVSQV VFREGDVLAA AHRLRAEFCV AVTGVVEVRP EGNENPEIPT GQIEVNATEL TVLGESAPLP FQLDEQAGEE ARLKYRYLDL RREGPGNALR LRSKVNAAAR SVLAEHDFVE IETPTLTRST PEGARDFLVP ARLQPGSFYA LPQSPQLFKQ LLMVAGMERY YQIARCYRDE DFRADRQPEF TQLDMEMSFV EADDVIAISE QVLKAVWATI GYDLPLPLPR ISYEEAMRRF GSDKPDLRFG IELVECTEYF KDTTFRVFQA PYVGAVVMXX GASQPRRTLD GWQEFAKQRG HKGLAYVLVG EDGTLGGPVA KNLSDAERDG AGRARRRQPG
Validated NT Sequence
ttgacttggc gtcttccttc ggcttgtctt ccctgggttt ggcgtcgatc ccggattcct tgcgctgctg cggcgtgatc ggtgcgggcg cgtcggtcag cgggtcgacc ccgccaccgg acttggggaa cgcgatgacc tcgcggatcg aatcgacccc ggcgagcagt gcggtgatgc gatcccagcc gaacgcgatg ccgccgtgcg gcggggcgcc gtaggagaac gcgtcgagca ggaacccgaa cttctcctgg gcctcgtcgt gatcgatgcc catcatcgcg aacacccgct cctggatgtc acggcggtgg atacggatcg agccgccacc gatctcgttg ccgttgcaca cgatgtcgta ggcgtcggac agcgcgttgc cgggatcgga gtcgaacgtg tcgacggagt cgggcttggg cgcggtgaac gcgtggtgca tcgcggtcca ggcgcccgag ccgacggcga cgtcaccggc ggccgtggcc tcgtcggcgg cctcgaacat cgggaagtcg accacccagg tgaacgccca ggcgttcggg tcgatgaggt cgaggcgctt ggcgatctcg atgcgggtgg cgccgagcag cgcgcgggca cccttggcgg gacctgccgc gaagaagatg cagtcacccg ggttggcgcc gacgtgcgcg accagccccg tcgcgttcgg cgtcggacag gttcttggcc accggcccgc cgagggttcc gtcctcgccg acgagcacgt acgccaggcc cttgtgcccg cgctgcttgg cgaactcctg ccagccgtcg agcgtgcggc ggggctgcga cgcgccgcnn gncatcacga cggcgccgac gtacggcgcc tggaaaacgc ggaacgtggt gtctttgaag tactcggtgc actcgacgag ttcgatgccg aagcgcagat cgggcttgtc ggagccgaag cggcgcatgg cctcttcgta gctgatccgc ggaagcggca gcggcaggtc gtagccgatc gtggcccaca cggccttgag gacctgctcg gagatcgcga tgacgtcgtc ggcctcgacg aagctcatct ccatgtcgag ctgggtgaac tcgggctgac gatcggcgcg gaagtcctcg tcgcggtagc agcgtgcgat ctggtagtag cgctccatgc ccgcgaccat cagcagctgc ttgaacagct gcgggctctg cggcagcgcg tagaacgagc cgggctgcag gcgcgcgggc accaggaagt cgcgggcgcc ctcgggcgtc gatcgcgtca gcgtcggggt ctcgatctcg acgaagtcgt gttcggcgag cacggaccgt gcggcggcgt tgaccttcga ccgcagccgc agtgcgttgc cggggccttc gcggcgcagg tccaggtagc ggtacttcag gcgcgcttcc tcgccggcct gctcgtcgag ctggaacggc aacggcgcgc tctcgcccag cacggtcagc tcggtggcgt tgacctcgat ctgcccggtg gggatctcgg ggttctcgtt gccctcggga cggacctcga cgacgccggt caccgcgacg cagaactcgg cgcgcagccg gtgcgcggcc gcgaggacgt cgccctcgcg gaacaccacc tgcgacaccc cggacgcgtc gcgcaggtcg atgaagatga cgccgccgtg atcacggcga cgcgccaccc atcccgccaa cgtcaccgtc tgaccggcgt cggcgggccg caacgaaccg gcggcatgag tgcgcagcac catcgaacca ggaccctggg tctgagcttc cagggtaccc atatgatggt gatggtgatg agccat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVLRTHAAG SLRPADAGQT VTLAGWVARR RDHGGVIFID LRDASGVSQV VFREGDVLAA AHRLRAEFCV AVTGVVEVRP EGNENPEIPT GQIEVNATEL TVLGESAPLP FQLDEQAGEE ARLKYRYLDL RREGPGNALR LRSKVNAAAR SVLAEHDFVE IETPTLTRST PEGARDFLVP ARLQPGSFYA LPQSPQLFKQ LLMVAGMERY YQIARCYRDE DFRADRQPEF TQLDMEMSFV EADDVIAISE QVLKAVWATI GYDLPLPLPR ISYEEAMRRF GSDKPDLRFG IELVECTEYF KDTTFRVFQA PYVGAVVMPG GASQPRRTLD GWQEFAKQRG HKGLAYVLVG EDGTLGGPVA KNLSDAERDG LVAHVGANPG DCIFFAAGPA KGARALLGAT RIEIAKRLDL IDPNAWAFTW VVDFPMFEAA DEATAAGDVA VGSGAWTAMH HAFTAPKPDS VDTFDSDPGN ALSDAYDIVC NGNEIGGGSI RIHRRDIQER VFAMMGIDHD EAQEKFGFLL DAFSYGAPPH GGIAFGWDRI TALLAGVDSI REVIAFPKSG GGVDPLTDAP APITPQQRKE SGIDAKPRED KPKEDAKSKA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgctgcgc actcatgccg ccggttcgtt gcggcccgcc gacgccggtc agacggtgac gttggcggga tgggtggcgc gtcgccgtga tcacggcggc gtcatcttca tcgacctgcg cgacgcgtcc ggggtgtcgc aggtggtgtt ccgcgagggc gacgtcctcg cggccgcgca ccggctgcgc gccgagttct gcgtcgcggt gaccggcgtc gtcgaggtcc gtcccgaggg caacgagaac cccgagatcc ccaccgggca gatcgaggtc aacgccaccg agctgaccgt gctgggcgag agcgcgccgt tgccgttcca gctcgacgag caggccggcg aggaagcgcg cctgaagtac cgctacctgg acctgcgccg cgaaggcccc ggcaacgcac tgcggctgcg gtcgaaggtc aacgccgccg cacggtccgt gctcgccgaa cacgacttcg tcgagatcga gaccccgacg ctgacgcgat cgacgcccga gggcgcccgc gacttcctgg tgcccgcgcg cctgcagccc ggctcgttct acgcgctgcc gcagagcccg cagctgttca agcagctgct gatggtcgcg ggcatggagc gctactacca gatcgcacgc tgctaccgcg acgaggactt ccgcgccgat cgtcagcccg agttcaccca gctcgacatg gagatgagct tcgtcgaggc cgacgacgtc atcgcgatct ccgagcaggt cctcaaggcc gtgtgggcca cgatcggcta cgacctgccg ctgccgcttc cgcggatcag ctacgaagag gccatgcgcc gcttcggctc cgacaagccc gatctgcgct tcggcatcga actcgtcgag tgcaccgagt acttcaaaga caccacgttc cgcgttttcc aggcgccgta cgtcggcgcc gtcgtgatgc ccggcggcgc gtcgcagccc cgccgcacgc tcgacggctg gcaggagttc gccaagcagc gcgggcacaa gggcctggcg tacgtgctcg tcggcgagga cggaaccctc ggcgggccgg tggccaagaa cctgtccgac gccgaacgcg acgggctggt cgcgcacgtc ggcgccaacc cgggtgactg catcttcttc gcggcaggtc ccgccaaggg tgcccgcgcg ctgctcggcg ccacccgcat cgagatcgcc aagcgcctcg acctcatcga cccgaacgcc tgggcgttca cctgggtggt cgacttcccg atgttcgagg ccgccgacga ggccacggcc gccggtgacg tcgccgtcgg ctcgggcgcc tggaccgcga tgcaccacgc gttcaccgcg cccaagcccg actccgtcga cacgttcgac tccgatcccg gcaacgcgct gtccgacgcc tacgacatcg tgtgcaacgg caacgagatc ggtggcggct cgatccgtat ccaccgccgt gacatccagg agcgggtgtt cgcgatgatg ggcatcgatc acgacgaggc ccaggagaag ttcgggttcc tgctcgacgc gttctcctac ggcgccccgc cgcacggcgg catcgcgttc ggctgggatc gcatcaccgc actgctcgcc ggggtcgatt cgatccgcga ggtcatcgcg ttccccaagt ccggtggcgg ggtcgacccg ctgaccgacg cgcccgcacc gatcacgccg cagcagcgca aggaatccgg gatcgacgcc aaacccaggg aagacaagcc gaaggaagac gccaagtcaa aggcgaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa