MythA.18629.a

Virulence factor Mce family protein (Rv0174 ortholog)

CENTER ID: MythA.18629.a
ORGANISM: Mycobacterium thermoresistibile ATCC 19527 / NCTC 10409
ASSOCIATED DISEASE: Respiratory Infection
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MythA.18629.a.B2.GE37660 mature protein 22 453
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|1078020.3.peg.497
RefSeq: WP_003923930.1
UniProt: G7CC11

Orthologues for Rv1971

Species % Identity % Coverage Clones Proteins Structures
fortuitum 63 97 0 0 0
thermoresistibile 60 100 1 0 0
avium 58 94 0 0 0
paratuberculosis 56 100 1 0 0
avium 55 99 1 0 0
fortuitum 28 66 0 0 0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLHRRIKIQL VIFAVIAMTA MALMSTQYMR LPAKLFGIGR YSVIVQLPEA AGLYPRGNVT YRGVEVGIVD SLHLTDTGVD AVLSLKSGID IPSDVTAEVH SQSAIGEQYV MLLPRDGTSP PLKDGDVIPP EQTRVPADIN AILADAVTGL QAVPKDGLQT LIDESYTAVG GLGPELRRIV SASIDLSLDA RDNLDPLVEL IEKSQPVLDS QTDTSQAIRA WASQVATVTD SLRNHDAQLA GVLHQGGPAF ADTRDLIDRL HPTLPSTVAH LATLGDVAHQ YHPAVEQLLV LFPQAIAEAQ ATHLANRNTE QDYRGQYLSF NLNLNLPPPC TTGFLPAQQQ RSPVHEDHPP REPGLLYCRT PQDSMWNVRG AKNYPCITRP GKRAPTAQMC ESDEHYIPLN DGNNWKGDPN ATLSGQDIPQ LPPGTSQTPP AEPGDPTTDP REQTWQSMLL PPR
NT Sequence
atgttgcacc ggagaatcaa gatccagctg gtgatcttcg cggtgatcgc gatgaccgcc atggcgttga tgtcgacgca gtacatgcgg ttgccggcga agctgttcgg catcggccgc tacagcgtga tcgtgcagtt gcccgaggcc gccgggctgt atccccgcgg caacgtcacc taccgcgggg tcgaggtcgg catcgtcgat tccctgcacc tgaccgacac cggggtcgac gcggtgttgt cgctgaaatc gggcatcgac atcccctccg acgtcaccgc cgaggtgcac agccagtccg cgatcggcga gcagtacgtg atgttgttgc cgcgggacgg gacctcacca ccgctgaagg acggcgatgt catcccaccc gagcagaccc gggtacccgc cgacatcaac gccatcctcg ccgacgcggt caccgggctg caggccgtgc ccaaagacgg tctgcagacc ctcatcgacg agtcctacac cgcggtaggc ggtctcggac ccgaactacg ccgcatcgtc agcgcctcga tcgacctgtc gctcgacgcg cgcgacaacc tcgacccgct ggtcgaattg atcgagaagt cccaaccggt gctggactca cagaccgaca cctcccaggc gatccgggcg tgggcctcgc aggtggccac cgtcaccgac tcgctgcgca accacgacgc gcaactggcc ggcgttctgc accagggcgg acccgcgttc gccgacaccc gcgacctgat cgaccgactc caccccacgc tgccgtccac cgtggcgcac ctggccaccc tcggcgacgt ggcacaccaa taccacccgg ccgtcgaaca actgctggtg ctgttccccc aggccatcgc cgaggcccag gccacccacc tggccaaccg caacaccgaa caggactacc gcggccaata cctcagcttc aacctcaacc tcaacctgcc accgccgtgc accaccggat tcctgcccgc acaacaacaa cgctcgcccg tccacgaaga ccatccacca cgcgaacccg gcctgctgta ctgccgcaca ccgcaggact cgatgtggaa cgtgcggggc gccaagaact atccgtgcat caccaggccc ggcaaacgcg cacccacggc gcagatgtgc gagagcgacg aacactacat acccctcaac gacggcaaca actggaaggg cgaccccaac gccaccctgt ccggacaaga catcccccaa ctgccgcccg gcacctccca gacgccgccc gccgagccgg gcgacccgac caccgaccca cgggagcaga catggcagtc gatgctgctg ccgccgcggt ag
Details for MythA.18629.a.B2.GE37660
HARVESTED ON: 1/13/2014
SEQUENCED ON: 1/9/2014
EXPECTED MW: 48kDa
OBSERVED MW: 49kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 85
PERCENT COVERAGE: 92
Validated AA Sequence
MAHHHHHHMA LMSTQYMRLP AKLFGIGRYS VIVQLPEAAG LYPRGNVTYR GVEVGIVDSL HLTDTGVDAV LSLKSGIDIP SDVTAEVHSQ SAIGEQYVML LPRDGTSPPL KDGDVIPPEQ TRVPADINAI LADAVTGLQA VPKDGLQTLI DESYTAVGGL GPELRRIVSA SIDLSLDARD NLDPLVELIE KSQPVLDSQT DTSQAIRAWA SQVATVTDSL RNHDAQLAGV LHQGGPAFAD TRDLIDRLHP TLPSTVAHLA TLGDVAHQYH PAVEQLLVLF PQAIAEAQAT HLANRNTEQD YRGQYLSFNL NLNLPPPCTT GFLPAHNNAR PSTNHPPREP GLLYCRTPXX RXXXAGAXXX XASXXGNXXX XXXCXXXXXX XXLTXXXXGD PXHXRXXXXX XXXXPXXXG
Validated NT Sequence
cnnnnnnntt cnctagatat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcgt tgatgtcgac gcagtacatg cggttgccgg cgaagctgtt cggcatcggc cgctacagcg tgatcgtgca gttgcccgag gccgccgggc tgtatccccg cggcaacgtc acctaccgcg gggtcgaggt cggcatcgtc gattccctgc acctgaccga caccggggtc gacgcggtgt tgtcgctgaa atcgggcatc gacatcccct ccgacgtcac cgccgaggtg cacagccagt ccgcgatcgg cgagcagtac gtgatgttgt tgccgcggga cgggacctca ccaccgctga aggacggcga tgtcatccca cccgagcaga cccgggtacc cgccgacatc aacgccatcc tcgccgacgc ggtcaccggg ctgcaggccg tgcccaaaga cggtctgcag accctcatcg acgagtccta caccgcggta ggcggtctcg gacccgaact acgccgcatc gtcagcgcct cgatcgacct gtcgctcgac gcgcgcgaca acctcgaccc gctggtcgaa ttgatcgaga agtcccaacc ggtgctggac tcacagaccg acacctccca ggcgatccgg gcgtgggcct cgcaggtggc caccgtcacc gactcgctgc gcaaccacga cgcgcaactg gccggcgttc tgcaccaggg cggacccgcg ttcgccgaca cccgcgacct gatcgaccga ctccacccca cgctgccgtc caccgtggcg cacctggcca ccctcggcga cgtggcacac caataccacc cggccgtcga acaactgctg gtgctgttcc cccaggccat cgccgaggcc caggccaccc acctggccaa ccgcaacacc gaacaggact accgcggcca atacctcagc ttcaacctca acctcaacct gccaccgccg tgcaccaccg gattcctgcc cgcacacaac aacgctcgcc cgtccacgaa ccatccacca cgcgaacccg gcctgctgta ctgccgcaca ccgcannctc gatgnngnng tgcgggggcg ncnnantanc ntgcatcacn gnccggcaac gnnnnncnng nnnaaatntg cnannncnac naanannnnn ntnccctnac gangnnnnnt nnnggngacc cnnnncacnn ncgnnannan nccnnnnnnc ntnnnnannc nccnncnnnn nggggaac
Expected Protein Sequence
MAHHHHHHAL MSTQYMRLPA KLFGIGRYSV IVQLPEAAGL YPRGNVTYRG VEVGIVDSLH LTDTGVDAVL SLKSGIDIPS DVTAEVHSQS AIGEQYVMLL PRDGTSPPLK DGDVIPPEQT RVPADINAIL ADAVTGLQAV PKDGLQTLID ESYTAVGGLG PELRRIVSAS IDLSLDARDN LDPLVELIEK SQPVLDSQTD TSQAIRAWAS QVATVTDSLR NHDAQLAGVL HQGGPAFADT RDLIDRLHPT LPSTVAHLAT LGDVAHQYHP AVEQLLVLFP QAIAEAQATH LANRNTEQDY RGQYLSFNLN LNLPPPCTTG FLPAQQQRSP VHEDHPPREP GLLYCRTPQD SMWNVRGAKN YPCITRPGKR APTAQMCESD EHYIPLNDGN NWKGDPNATL SGQDIPQLPP GTSQTPPAEP GDPTTDPREQ TWQSMLLPPR
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgcgttga tgtcgacgca gtacatgcgg ttgccggcga agctgttcgg catcggccgc tacagcgtga tcgtgcagtt gcccgaggcc gccgggctgt atccccgcgg caacgtcacc taccgcgggg tcgaggtcgg catcgtcgat tccctgcacc tgaccgacac cggggtcgac gcggtgttgt cgctgaaatc gggcatcgac atcccctccg acgtcaccgc cgaggtgcac agccagtccg cgatcggcga gcagtacgtg atgttgttgc cgcgggacgg gacctcacca ccgctgaagg acggcgatgt catcccaccc gagcagaccc gggtacccgc cgacatcaac gccatcctcg ccgacgcggt caccgggctg caggccgtgc ccaaagacgg tctgcagacc ctcatcgacg agtcctacac cgcggtaggc ggtctcggac ccgaactacg ccgcatcgtc agcgcctcga tcgacctgtc gctcgacgcg cgcgacaacc tcgacccgct ggtcgaattg atcgagaagt cccaaccggt gctggactca cagaccgaca cctcccaggc gatccgggcg tgggcctcgc aggtggccac cgtcaccgac tcgctgcgca accacgacgc gcaactggcc ggcgttctgc accagggcgg acccgcgttc gccgacaccc gcgacctgat cgaccgactc caccccacgc tgccgtccac cgtggcgcac ctggccaccc tcggcgacgt ggcacaccaa taccacccgg ccgtcgaaca actgctggtg ctgttccccc aggccatcgc cgaggcccag gccacccacc tggccaaccg caacaccgaa caggactacc gcggccaata cctcagcttc aacctcaacc tcaacctgcc accgccgtgc accaccggat tcctgcccgc acaacaacaa cgctcgcccg tccacgaaga ccatccacca cgcgaacccg gcctgctgta ctgccgcaca ccgcaggact cgatgtggaa cgtgcggggc gccaagaact atccgtgcat caccaggccc ggcaaacgcg cacccacggc gcagatgtgc gagagcgacg aacactacat acccctcaac gacggcaaca actggaaggg cgaccccaac gccaccctgt ccggacaaga catcccccaa ctgccgcccg gcacctccca gacgccgccc gccgagccgg gcgacccgac caccgaccca cgggagcaga catggcagtc gatgctgctg ccgccgcggt gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat