MyavA.18629.b

Virulence factor mce family protein (Rv0174 ortholog)

CENTER ID: MyavA.18629.b
ORGANISM: Mycobacterium avium 104
ASSOCIATED DISEASE: Johne's disease, paratuberculosis
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyavA.18629.b.B2.GE37421 mature protein 24 486
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|243243.7.peg.2018
RefSeq: YP_881285.1
UniProt: A0A0H3A2W4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNRRTWIQLS ILALVTVVSC GAMAFNFMKL PATLFGIGEY RVTVDLPQSG GLYQTSVVTY RGTDVGQVKS VDVTATGVRA VLAMRSGIEV PFDVQASVHS RSAIGEQYIE LTPRPGTGDH QSRTLRDGDV ITAGRVEVPV DIGRLLDMTN RALQAIPRDN LRTVIDETNR AVGGLGPELS RIVDGSTALA VAGGHTVDPL ATLIDQSPAV LNSQVQTSGA IATWAERTAA LMAQFKAQDA AVRDLLAHGA SGVEEGRALL DRVTPALPVL FANLVSLGDI AVVYRHDIEQ LLVLLPQGIA AMAAIIVPSS NTKQEYRGAQ LDFNLNLNLP PPCTTGYLPP AQRRSPASVD APDRPAADLY CRVPQDSEFN VRGVRNIPCE SKPWKRAPTV ELCESDEEYV PLNDGYNWKG DPNATTSGQG VPQYPPGQDP RLPPPRGTAP APPPVAVAPY DPATGDYIGP DGRRYTESDL AHPRAKSWQS LLVPQP
NT Sequence
ttgaaccgcc ggacctggat ccagttgtcg attctggcgc tggtgaccgt tgtttcctgc ggcgcaatgg cattcaactt catgaagctg cccgcgaccc tgttcgggat cggcgagtac cgggtcacgg tcgacctgcc gcaatcgggt gggctctacc agacctcggt ggtcacctac cgcggcaccg acgtcggcca ggtcaaatcg gtcgacgtca ccgcgaccgg agtgcgcgcg gtgctggcta tgcggtcggg gatcgaggtg cccttcgacg tccaggcgtc ggtgcacagc cgctcggcga tcggtgagca gtacatcgag ctgaccccgc ggcccggcac cggcgaccac cagtcccgga cgctgcgcga cggcgacgtc atcaccgccg gccgcgtcga ggtaccggtg gacatcgggc ggctgctcga catgaccaac cgtgcgctgc aagcgattcc ccgcgacaac ctgcgcacgg tgatcgacga gaccaaccgg gcggtcggcg gactgggccc cgagttgtcc cgcatcgtcg acgggtccac cgcgctggct gtcgccggcg gccacaccgt cgacccgctc gccacgctga tcgatcaatc tccggcggtg ctcaactcac aggtccagac ctccggggcg atcgccacct gggccgagcg caccgcggcc ctcatggcgc agttcaaggc gcaggatgcc gcggtgcgag acctgttggc gcacggcgct tccggagtcg aggaggggcg cgccctgctg gaccgggtga cgcccgccct gccggtgctg ttcgccaacc tggtgagcct gggcgacatc gccgtcgtct accgccacga catcgaacaa ctgctggtgt tgctgccgca gggcatcgcg gccatggcgg cgatcatcgt gcccagctcc aacaccaagc aggagtaccg gggcgcccag ttggacttca acctcaacct caacctgccg ccgccgtgca cgacgggtta tctgcctccg gcgcaacgcc gttcgcccgc cagcgtcgac gcgccggacc gaccggccgc cgacttgtat tgccgggtgc cgcaggactc ggaattcaac gtccgcggcg tgcgcaacat cccctgtgag agcaagccgt ggaaacgcgc accgaccgtc gaactgtgcg aaagcgacga agaatacgtg ccgctcaacg acggctacaa ctggaagggc gaccccaatg ccaccaccag tggtcaaggc gtgccgcagt atccgcccgg acaggatccg cggctgccgc caccgcgggg caccgccccg gcacccccgc ccgtcgcggt ggcgccctac gacccggcca ccggggatta catcgggccg gacggccgcc gctacaccga gtccgatctg gcgcacccac gcgccaaaag ctggcaatca cttctggttc cgcaaccctg a
Details for MyavA.18629.b.B2.GE37421
HARVESTED ON: 9/27/2013
SEQUENCED ON: 10/3/2013
EXPECTED MW: 51kDa
OBSERVED MW: 51kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 71
PERCENT COVERAGE: 74
Validated AA Sequence
MKLPATLFGI GEYRVTVDLP QSGGLYQTSV VTYRGTDVGQ VKSVDVTATG VRAVLAMRSG IEVPFDVQAS VHSRSAIGEQ YIELTPRPGT GDHQSRTLRD GDVITAGRVE VPVDIGRLLD MTNRALQAIP RDNLRTVIDE TNRAVGGLGP ELSRIVDGST ALAVAGGHTV DPLATLIDQS PAVLNSQVQT SGAIATWAER TAAPHGAVQG AGCRGARPVG ARRFRSRGGA RPAGPGDARP AGAVRQPGEP GRHRRRLPPR HRTTAGVAAA GHRGHGGDHR AQLQHQAGVP GRPVGLQPQP QPAAAVHDGL SASGATPFAR QRRRAGPTGR RLVLPGAAGL GIQRPRRAQH PL
Validated NT Sequence
cttttggcgc gtgggtgcgc cagatcggac tcggtgtagc ggcggccgtc cggcccgatg taatccccgg tggccgggtc gtagggcgcc accgcgacgg gcgggggtgc cggggcggtg ccccgcggtg gcggcagccg cggatcctgt ccgggcggat actgcggcac gccttgacca ctggtggtgg cattggggtc gcccttccag ttgtagccgt cgttgagcgg cacgtattct tcgtcgcttt cgcacagttc gacggtcggt gcgcgtttcc acggcttgct ctcacagggg atgttgcgca cgccgcggac gttgaattcc gagtcctgcg gcacccggca atacaagtcg gcggccggtc ggtccggcgc gtcgacgctg gcgggcgaac ggcgttgcgc cggaggcaga taacccgtcg tgcacggcgg cggcaggttg aggttgaggt tgaagtccaa ctgggcgccc cggtactcct gcttggtgtt ggagctgggc acgatgatcg ccgccatggc cgcgatgccc tgcggcagca acaccagcag ttgttcgatg tcgtggcggt agacgacggc gatgtcgccc aggctcacca ggttggcgaa cagcaccggc agggcgggcg tcacccggtc cagcagggcg cgcccctcct cgactccgga agcgccgtgc gccaacaggt ctcgcaccgc ggcatcctgc gccttgaact gcgccatgag gggccgcggt gcgctcggcc caggtggcga tcgccccgga ggtctggacc tgtgagttga gcaccgccgg agattgatcg atcagcgtgg cgagcgggtc gacggtgtgg ccgccggcga cagccagcgc ggtggacccg tcgacgatgc gggacaactc ggggcccagt ccgccgaccg cccggttggt ctcgtcgatc accgtgcgca ggttgtcgcg gggaatcgct tgcagcgcac ggttggtcat gtcgagcagc cgcccgatgt ccaccggtac ctcgacgcgg ccggcggtga tgacgtcgcc gtcgcgcagc gtccgggact ggtggtcgcc ggtgccgggc cgcggggtca gctcgatgta ctgctcaccg atcgccgagc ggctgtgcac cgacgcctgg acgtcgaagg gcacctcgat ccccgaccgc atagccagca ccgcgcgcac tccggtcgcg gtgacgtcga ccgatttgac ctggccgacg tcggtgccgc ggtaggtgac caccgaggtc tggtagagcc cacccgattg cggcaggtcg accgtgaccc ggtactcgcc gatcccgaac agggtcgcgg gcagcttcat gaagttgaat g
Expected Protein Sequence
MAHHHHHHAF NFMKLPATLF GIGEYRVTVD LPQSGGLYQT SVVTYRGTDV GQVKSVDVTA TGVRAVLAMR SGIEVPFDVQ ASVHSRSAIG EQYIELTPRP GTGDHQSRTL RDGDVITAGR VEVPVDIGRL LDMTNRALQA IPRDNLRTVI DETNRAVGGL GPELSRIVDG STALAVAGGH TVDPLATLID QSPAVLNSQV QTSGAIATWA ERTAALMAQF KAQDAAVRDL LAHGASGVEE GRALLDRVTP ALPVLFANLV SLGDIAVVYR HDIEQLLVLL PQGIAAMAAI IVPSSNTKQE YRGAQLDFNL NLNLPPPCTT GYLPPAQRRS PASVDAPDRP AADLYCRVPQ DSEFNVRGVR NIPCESKPWK RAPTVELCES DEEYVPLNDG YNWKGDPNAT TSGQGVPQYP PGQDPRLPPP RGTAPAPPPV AVAPYDPATG DYIGPDGRRY TESDLAHPRA KSWQSLLVPQ P
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgcattca acttcatgaa gctgcccgcg accctgttcg ggatcggcga gtaccgggtc acggtcgacc tgccgcaatc gggtgggctc taccagacct cggtggtcac ctaccgcggc accgacgtcg gccaggtcaa atcggtcgac gtcaccgcga ccggagtgcg cgcggtgctg gctatgcggt cggggatcga ggtgcccttc gacgtccagg cgtcggtgca cagccgctcg gcgatcggtg agcagtacat cgagctgacc ccgcggcccg gcaccggcga ccaccagtcc cggacgctgc gcgacggcga cgtcatcacc gccggccgcg tcgaggtacc ggtggacatc gggcggctgc tcgacatgac caaccgtgcg ctgcaagcga ttccccgcga caacctgcgc acggtgatcg acgagaccaa ccgggcggtc ggcggactgg gccccgagtt gtcccgcatc gtcgacgggt ccaccgcgct ggctgtcgcc ggcggccaca ccgtcgaccc gctcgccacg ctgatcgatc aatctccggc ggtgctcaac tcacaggtcc agacctccgg ggcgatcgcc acctgggccg agcgcaccgc ggccctcatg gcgcagttca aggcgcagga tgccgcggtg cgagacctgt tggcgcacgg cgcttccgga gtcgaggagg ggcgcgccct gctggaccgg gtgacgcccg ccctgccggt gctgttcgcc aacctggtga gcctgggcga catcgccgtc gtctaccgcc acgacatcga acaactgctg gtgttgctgc cgcagggcat cgcggccatg gcggcgatca tcgtgcccag ctccaacacc aagcaggagt accggggcgc ccagttggac ttcaacctca acctcaacct gccgccgccg tgcacgacgg gttatctgcc tccggcgcaa cgccgttcgc ccgccagcgt cgacgcgccg gaccgaccgg ccgccgactt gtattgccgg gtgccgcagg actcggaatt caacgtccgc ggcgtgcgca acatcccctg tgagagcaag ccgtggaaac gcgcaccgac cgtcgaactg tgcgaaagcg acgaagaata cgtgccgctc aacgacggct acaactggaa gggcgacccc aatgccacca ccagtggtca aggcgtgccg cagtatccgc ccggacagga tccgcggctg ccgccaccgc ggggcaccgc cccggcaccc ccgcccgtcg cggtggcgcc ctacgacccg gccaccgggg attacatcgg gccggacggc cgccgctaca ccgagtccga tctggcgcac ccacgcgcca aaagctggca atcacttctg gttccgcaac cctgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat