MypaA.18629.a

Putative uncharacterized protein (Rv0174 ortholog)

CENTER ID: MypaA.18629.a
ORGANISM: Mycobacterium paratuberculosis ATCC BAA-968 / K-10
ASSOCIATED DISEASE: Johne's disease
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MypaA.18629.a.B2.GE37491 mature protein 27 489
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|262316.17.peg.2240
RefSeq: NP_961045.1
UniProt: Q73Y46

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLRLNRRTWI QLSILALVTV VSCGAMAFNF MKLPATLFGI GEYRVTVDLP QSGGLYQTSV VTYRGTDVGQ VKSVDVTATG VRAVLAMRSG IEVPFDVQAS VHSRSAIGEQ YIELTPRPGT GDHQSRTLRD GDVITAGRVD VPVDIGRLLD MTNRALQAIP RDNLRTVIDE TNRAVGGLGP ELSRIVDGST ALAVAGGHTV DPLATLIDQS PAVLNSQVQT SGAIATWAER TAALMAQFKA QDAAVRDLLT RGTSGVEEGR ALLDRVTPAL PVLFANLVSL GDIAVVYRHD IEQLLVLLPQ GIAAMAAIIV PSSNTKQEYR GAQLDFNLNL NLPPPCTTGY LPPAQRRSPA SVDAPDRPAA DLYCRVPQDS EFNVRGVRNI PCESKPWKRA PTVELCESDE EYVPLNDGYN WKGDPNATTS GQGVPQYPPG QDPRLPPPRG TAPAPPPVAV APYDPATGDY IGPDGRRYTE SDLAHPRAKS WQSLLVPQP
NT Sequence
atgcttcggt tgaaccgccg gacctggatc cagttgtcga ttctggcgct ggtgaccgtt gtttcctgcg gcgcaatggc attcaacttc atgaagctgc ccgcgaccct gttcgggatc ggcgagtacc gggtcacggt cgacctgccg caatcgggtg ggctctacca gacctcggtg gtcacctacc gcggcaccga cgtcggccag gtcaaatcgg tcgacgtcac cgcgaccgga gtgcgcgcgg tgctggctat gcggtcgggg atcgaggtgc cattcgacgt ccaggcgtcg gtgcacagcc gctcggcgat cggtgagcag tacatcgagc tgaccccgcg gcccggcacc ggcgaccacc agtcccggac gctgcgcgac ggcgacgtca tcaccgccgg ccgcgtcgac gtacccgtcg acatcgggcg gctgctcgac atgaccaacc gtgcgctgca agcgattccc cgcgacaacc tgcgcacggt gatcgacgag accaaccggg cggtcggcgg actgggcccc gagttgtccc gcatcgtcga cgggtccacc gcgctggccg tcgccggcgg ccacaccgtc gacccgctcg ccacgctgat cgatcaatct ccggcggtgc tcaactcaca ggtccagacc tccggggcga tcgccacctg ggccgagcgc accgcggccc tcatggcgca gttcaaggcg caggatgccg cggtgcgaga cctgttgacg cgcggcactt ccggagtgga ggaggggcgc gccctgctgg accgggtgac gcccgccctg ccggtgctgt tcgccaacct ggtgagcctg ggcgacatcg ccgtcgtcta ccgccacgac atcgaacaac tgctggtgtt gctgccacag ggcatcgcgg ccatggcggc gatcatcgtg cccagctcca acaccaagca ggagtaccgg ggcgcccagt tggacttcaa cctcaacctc aacctgccgc cgccgtgcac gacgggttat ctgcctccgg cgcaacgccg ttcgcccgcc agcgtcgacg cgccggaccg acccgccgcc gacttgtatt gccgggtgcc gcaggactcg gaattcaacg tccgcggcgt gcgcaacatc ccctgtgaga gcaagccgtg gaaacgcgca ccgaccgtcg aactgtgcga aagcgacgaa gaatacgtgc cgctcaacga cggctacaac tggaagggcg accccaatgc caccaccagt ggtcaaggcg tgccgcagta tccgccggga caggatccgc ggctgccgcc accgcgcggc accgccccgg cacccccgcc cgtcgcggtg gcgccctacg acccggccac cggggattac atcgggccgg acggccgccg ctacaccgag tccgatttgg cgcacccacg cgccaaaagc tggcaatcac ttctggttcc gcaaccctga
Details for MypaA.18629.a.B2.GE37491
HARVESTED ON: 10/31/2013
SEQUENCED ON: 11/5/2013
EXPECTED MW: 51kDa
OBSERVED MW: 51kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 82
PERCENT COVERAGE: 82
Validated AA Sequence
MAHHHHHHMA FNFMKLPATL FGIGEYRVTV DLPQSGGLYQ TSVVTYRGTD VGQVKSVDVT ATGVRAVLAM RSGIEVPFDV QASVHSRSAI GEQYIELTPR PGTGDHQSRT LRDGDVITAG RVDVPVDIGR LLDMTNRALQ AIPRDNLRTV IDETNRAVGG LGPELSRIVD GSTALAVAGG HTVDPLATLI DQSPAVLNSQ VQTSGAIATW AERTAALMAQ FKAXDAAVRD LLTRGTSGVE EGRALLDRVT PALPVLFANL VSLGDIAVVY RHDIEQLLVL LPQGIAAMAA IIVPSSXTKQ EYRGAXLDFX LXLXLPPPCT XGYLXXXNXX RAXXXXXXRX XXXXIXXXXX XXXXXXXXXP XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX
Validated NT Sequence
ttttgtttaa ctttangaag gaganatacc atggctcacc accaccacca ccatatggca ttcaacttca tgaagctgcc cgcgaccctg ttcgggatcg gcgagtaccg ggtcacggtc gacctgccgc aatcgggtgg gctctaccag acctcggtgg tcacctaccg cggcaccgac gtcggccagg tcaaatcggt cgacgtcacc gcgaccggag tgcgcgcggt gctggctatg cggtcgggga tcgaggtgcc attcgacgtc caggcgtcgg tgcacagccg ctcggcgatc ggtgagcagt acatcgagct gaccccgcgg cccggcaccg gcgaccacca gtcccggacg ctgcgcgacg gcgacgtcat caccgccggc cgcgtcgacg tacccgtcga catcgggcgg ctgctcgaca tgaccaaccg tgcgctgcaa gcgattcccc gcgacaacct gcgcacggtg atcgacgaga ccaaccgggc ggtcggcgga ctgggccccg agttgtcccg catcgtcgac gggtccaccg cgctggccgt cgccggcggc cacaccgtcg acccgctcgc cacgctgatc gatcaatctc cggcggtgct caactcacag gtccagacct ccggggcgat cgccacctgg gccgagcgca ccgcggccct catggcgcag ttcaaggcgc angatgccgc ggtgcgagac ctgttgacgc gcggcacttc cggagtggag gaggggcgcg ccctgctgga ccgggtgacg cccgccctgc cggtgctgtt cgccaacctg gtgagcctgg gcgacatcgc cgtcgtctac cgccacgaca tcgaacaact gctggtgttg ctgccacagg gcatcgcggc catggcggcg atcatcgtgc ccagctccna caccaagcag gagtaccggg gcgccnagtt ggacttcanc ctcnncctca ncctgccgcc gccntgcacg angggttatc tgcnnnnnnn naacgncntt cgcgccnnnn nncnannnnn nnancgancn nnncnnantt nnattgnnng ncnnnnnntc nnnnncannn cnnngnnnng nnnnnntccc nnngnnanca nnnnnnnnaa nnnnnnncnn ncnnnnannn nnnnnannnn nnnannnnng nnnnannngn nnnggnnnnn ncncnnnnnn nnc
Expected Protein Sequence
MAHHHHHHAF NFMKLPATLF GIGEYRVTVD LPQSGGLYQT SVVTYRGTDV GQVKSVDVTA TGVRAVLAMR SGIEVPFDVQ ASVHSRSAIG EQYIELTPRP GTGDHQSRTL RDGDVITAGR VDVPVDIGRL LDMTNRALQA IPRDNLRTVI DETNRAVGGL GPELSRIVDG STALAVAGGH TVDPLATLID QSPAVLNSQV QTSGAIATWA ERTAALMAQF KAQDAAVRDL LTRGTSGVEE GRALLDRVTP ALPVLFANLV SLGDIAVVYR HDIEQLLVLL PQGIAAMAAI IVPSSNTKQE YRGAQLDFNL NLNLPPPCTT GYLPPAQRRS PASVDAPDRP AADLYCRVPQ DSEFNVRGVR NIPCESKPWK RAPTVELCES DEEYVPLNDG YNWKGDPNAT TSGQGVPQYP PGQDPRLPPP RGTAPAPPPV AVAPYDPATG DYIGPDGRRY TESDLAHPRA KSWQSLLVPQ P
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgcattca acttcatgaa gctgcccgcg accctgttcg ggatcggcga gtaccgggtc acggtcgacc tgccgcaatc gggtgggctc taccagacct cggtggtcac ctaccgcggc accgacgtcg gccaggtcaa atcggtcgac gtcaccgcga ccggagtgcg cgcggtgctg gctatgcggt cggggatcga ggtgccattc gacgtccagg cgtcggtgca cagccgctcg gcgatcggtg agcagtacat cgagctgacc ccgcggcccg gcaccggcga ccaccagtcc cggacgctgc gcgacggcga cgtcatcacc gccggccgcg tcgacgtacc cgtcgacatc gggcggctgc tcgacatgac caaccgtgcg ctgcaagcga ttccccgcga caacctgcgc acggtgatcg acgagaccaa ccgggcggtc ggcggactgg gccccgagtt gtcccgcatc gtcgacgggt ccaccgcgct ggccgtcgcc ggcggccaca ccgtcgaccc gctcgccacg ctgatcgatc aatctccggc ggtgctcaac tcacaggtcc agacctccgg ggcgatcgcc acctgggccg agcgcaccgc ggccctcatg gcgcagttca aggcgcagga tgccgcggtg cgagacctgt tgacgcgcgg cacttccgga gtggaggagg ggcgcgccct gctggaccgg gtgacgcccg ccctgccggt gctgttcgcc aacctggtga gcctgggcga catcgccgtc gtctaccgcc acgacatcga acaactgctg gtgttgctgc cacagggcat cgcggccatg gcggcgatca tcgtgcccag ctccaacacc aagcaggagt accggggcgc ccagttggac ttcaacctca acctcaacct gccgccgccg tgcacgacgg gttatctgcc tccggcgcaa cgccgttcgc ccgccagcgt cgacgcgccg gaccgacccg ccgccgactt gtattgccgg gtgccgcagg actcggaatt caacgtccgc ggcgtgcgca acatcccctg tgagagcaag ccgtggaaac gcgcaccgac cgtcgaactg tgcgaaagcg acgaagaata cgtgccgctc aacgacggct acaactggaa gggcgacccc aatgccacca ccagtggtca aggcgtgccg cagtatccgc cgggacagga tccgcggctg ccgccaccgc gcggcaccgc cccggcaccc ccgcccgtcg cggtggcgcc ctacgacccg gccaccgggg attacatcgg gccggacggc cgccgctaca ccgagtccga tttggcgcac ccacgcgcca aaagctggca atcacttctg gttccgcaac cctgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat